内39号葡萄株系的离体快繁技术研究   总被引：7，自引：0，他引：7  

曹孜义  杨德龙  梁庆丰  于作文 《果树学报》2002,19(6):427-429

利用一个新育鲜食葡萄内39号株系,在原离体快繁技术基础上,改进取材时间和繁殖技术,同时配合试管苗绿枝扦插,经过9个月的繁殖,生产出2.32万株生产用苗。逐月统计了实际生产数,表明葡萄离体快繁技术确实可缩短育种年限,用于育种实践。  相似文献   

Human and bovine respiratory syncytial virus vaccine research and development     

Meyer G  Deplanche M  Schelcher F 《Comparative immunology, microbiology and infectious diseases》2008,31(2-3):191-225

Human (HRSV) and bovine (BRSV) respiratory syncytial viruses (RSV) are two closely related viruses, which are the most important causative agents of respiratory tract infections of young children and calves, respectively. BRSV vaccines have been available for nearly 2 decades. They probably have reduced the prevalence of RSV infection but their efficacy needs improvement. In contrast, despite decades of research, there is no currently licensed vaccine for the prevention of HRSV disease. Development of a HRSV vaccine for infants has been hindered by the lack of a relevant animal model that develops disease, the need to immunize immunologically immature young infants, the difficulty for live vaccines to find the right balance between attenuation and immunogenicity, and the risk of vaccine-associated disease. During the past 15 years, intensive research into a HRSV vaccine has yielded vaccine candidates, which have been evaluated in animal models and, for some of them, in clinical trials in humans. Recent formulations have focused on subunit vaccines with specific CD4+ Th-1 immune response-activating adjuvants and on genetically engineered live attenuated vaccines. It is likely that different HRSV vaccines and/or combinations of vaccines used sequentially will be needed for the various populations at risk. This review discusses the recent advances in RSV vaccine development.  相似文献   

茶树扦插繁育综合技术研究   总被引：1，自引：0，他引：1  

徐泽  李中林  胡翔  彭萍  侯渝嘉 《西南园艺》2005,33(1):4-6

选用适宜西南茶区种植的名优绿茶良种早白尖5号、南江1号、渝茶一号为试验材料,按照4因素3水平正交试验设计进行短穗扦插繁育试验。结果表明,茶树品种间发根能力强弱顺序为早白尖5号>南江1号>渝茶一号,茶苗出圃率顺序为早白尖5号>渝茶一号>南江1号。配套综合扦插技术早白尖5号宜采用扦插土消毒、苗床遮荫、促根剂处理插穗、地膜覆盖,南江1号宜采用扦插土消毒、苗床遮荫、覆盖50cm高的天膜,渝茶一号宜采用扦插土消毒、苗床遮荫、促根剂处理插穗。  相似文献   

油菜病毒病的发生及防治对策   总被引：4，自引：0，他引：4  

夏宝远 《河北农业科学》2009,13(3):46-47

油菜是安徽省主要油料作物,病毒病是油菜的重要病害。随着油菜种植面积的扩大,油菜病毒病的发生越来越严重。总结了油菜病毒病的发生特点、流行规律及防治对策。  相似文献   

Identification of recombinant baculovirus and determination of virus titer with fluorescence quantitative PCR assay     

TONG Xia-sheng  MENG Zhe-feng△ 《园艺学报》2007,23(8):1623-1626

AIM: To develop a real-time PCR assay based on TaqMan technology for the identification of recombinant baculovirus and determination of virus physical titers in Bac-to-Bac system.METHODS: The recombinant baculovirus containing human IL-18 gene was produced using Bac-to-Bac system.A 10-fold serially diluted primary viral stock was used for plaque assay and DNA extraction.Bacmid (baculovirus plasmid) was 10-fold serially diluted and served as standards.Real-time PCR amplification of the IL-18 gene was performed in triplicate for each diluted recombinant virus.At the same time,plaque assays were performed using overlay agarose method.RESULTS: The standard linear range (101 to 108 copies) for quantitation was achieved with the standard curve.We also find that the“vg/mL”titer value is generally about 10 times than“pfu/mL”titer of the same recombinant virus stock.CONCLUSION: A TaqMan real-time PCR method is established to identify the recombinant baculovirus and determine the“vg/mL”titer of virus.The method is rapid and quantitative over a wide range of virus titers.  相似文献   

南蛇藤人工播种繁育试验研究   总被引：1，自引：0，他引：1  

张文庆 《中国林副特产》2003,(3):36-37

南蛇藤为药用植物，还可以用于工业、纺织业，是一种多用的经济植物．也是极有开发前途的绿化树种．近两年来对该植物进行了人工栽培繁育的试验研究，收到了显著的效果，其种子发芽率可速95%以上，种子催芽处理后播种对成苗率和苗高生长有极显著的影响．  相似文献   

三倍体毛白杨封顶蘸根扦插技术研究     

贾小明  张焕玲  周锋利 《陕西林业科技》2003,(4):17-21

本文主要讨论了石蜡封顶、激素(NAA、BA等)“蘸根”抑上促下技术对三倍体毛白杨扦插成活率、生根及生长特性的影响。研究表明：石蜡封顶可明显提高三倍体扦插成活率及扦插苗质量，处理较对照扦插成活率提高了29．4％，苗高提高了18．4％，胸径提高了17．4％。生长索可促进皮部生根，但对愈伤组织生根作用不明显。生长素中低比例的细胞分裂素可明显促进愈伤组织根的形成，含量高则起反作用。本文通过流水浸条、石蜡封顶、激素(NAA BA(2：1)(浓度250～750mg／kg))蘸根相结合的措施，将三倍体扦插成活率提高到65．4％的平均水平，最高达82．3％。  相似文献   

柿树离体繁殖研究     

孔祥生  马爱国  张益民  张妙霞 《河南科技大学学报(农学版)》1998,(3)

以成年柿树休眠芽为外植体，研究了影响柿树离体繁殖的有关因素。结果表明：附加ZT1～2mg／L、IAA0．05～0．1mg／L、叶酸1mg／L、D-泛酸钙1mg／L、LH100mg／L、PVP500mg／L的改良MS（KNO3和NH4NO3减半）培养基对柿芽的增殖和生长最为适宜。在继代培养中，适当缩短每代培养时间可加快新梢的增殖速度。IAA诱导生根的效果优于IBA，用生长素溶液浸新梢基部后转入1／2MS中对生根更有利。在较低温度下，以腐殖土作基质，试管苗的移栽成活率可达68．6％。  相似文献   

细叶野牡丹扦插繁殖技术研究     

林秀香 《西南林学院学报》2009,29(1):42-44

不同扦插基质、扦插季节和插条部位育苗比较试验结果表明：不同处理间扦插生根和根系生长均存在极显著影响，以红壤：谷壳灰=4：1为基质的扦插效果最好，生根率平均达98．15％，生根数为13．83条，最长根为11．01cm；四季均可扦插，但最佳季节是春季。不同部位不同季节处理组合间比较，最好的处理组合为春季＋顶部枝条＋红壤：谷壳灰=4：1，生根率平均达95．37％，生根数为15．67条。  相似文献   

放牧对冷蒿繁殖特性的影响     

祁永  杜丽霞  韩建国 《草地学报》2007,15(2):168-172

重度放牧对冷蒿(Artemisia frigida)的有性生殖具有严重的阻碍作用,严重影响其有性生殖;冷蒿生殖枝分化率、生殖枝花序数、种子产量、种子成熟度和发芽率之间在同一牧压下表现并不一致,在不同牧压下存在一定的平衡关系;放牧强度起调节作用是冷蒿对放牧的适应机制.  相似文献