Haematological and biochemical studies in broiler chicks fed ochratoxin and inoculated with inclusion body hepatitis virus,singly and in concurrence     

Sandhu  B.S.  Singh  B.  Brar  R.S. 《Veterinary research communications》1998,22(5):335-346

Day-old broiler chicks, which had been shown to be negative for maternal antibodies against inclusion body hepatitis (IBH) virus and for viral antigen in cloacal swabs, were divided into four groups of 20 chicks each. One group was fed ochratoxin-A at 0.5 ppm from 3 to 38 days of age, another group was inoculated with 1 ml of IBH virus containing 10^6.5 EID₅₀ per 0.2 ml. A third group was given both ochratoxin-A and infected with IBH virus. The fourth group served as the control. Anaemia was observed in all three treated groups but the changes were more pronounced in the combined group. The biochemical changes also suggested a cumulative damaging effect by ochratoxin-A and IBH virus.  相似文献   

Epidemiological investigation of infectious salmon anaemia (ISA) outbreaks in Norway 2003-2005     

Lyngstad TM  Jansen PA  Sindre H  Jonassen CM  Hjortaas MJ  Johnsen S  Brun E 《Preventive veterinary medicine》2008,84(3-4):213-227

Epidemiological information was summarized from 32 outbreaks of infectious salmon anaemia (ISA) on salmon farming sites in Norway in 2003–2005. Virus isolates from the outbreak sites were genotyped, and the genotyping was used to assess possible associations between outbreak sites due to adjacent location, sharing fish farming authorisation, sharing smolt suppliers or sharing broodfish origin of the fish. The ISA outbreaks were distributed along most of the Norwegian coast and showed a variable clinical picture. The virus genotypes clustered into three genogroups. Pairs of outbreak sites matched for adjacent location or registered under the same authorisation, all shared genogroup, which was a significantly higher number of corresponding genogroups than expected by chance. For outbreak sites sharing smolt suppliers, corresponding genogroups appeared in 7 out of 12 matched pairs, which was not significant. An evaluation of broodfish origin associated with genogroups did not support transmission linked to broodfish origin. In conclusion, genotyping of virus isolates from ISA outbreaks supports associations between adjacent outbreaks. This is consistent with horizontal transmission. The present study failed to find evidence for vertical transmission (patterns of genogroups related to smolt suppliers or broodfish companies were not identified).  相似文献   

用地高辛探针对江苏省鸡传染性贫血病进行流行病学调查   总被引：5，自引：0，他引：5  

张知良  邹尧坤 《中国兽药杂志》2000,34(4):8-9

用地高辛标记的核酸（ＤＮＡ）探针对的扬州、江都、连云港、苏州、无锡等５蛭９个养鸡场（户）随机取样１０６份（分别为１２￣６０日龄的肉鸡、蛋鸡和草鸡,采集胸腺、法氏囊和脾脏组织,抽提ＤＮＡ）进行检测ＣＡＶ感染情况,ＣＡＶ阳性鸡２２份阳必率为２０．７％,各鸡场阳性率人５￣６０％不等。结果表明,在处的一些地区鸡群中ＣＡＶ的感染已非常普遍,有的地区甚至十分严重,控制该病的发生已刻不容缓。  相似文献   

Expression of iron regulatory proteins in full-term swine placenta     

Kandasamy Rajamanickam  Venkatasubramanian Leela  Gopalakrishnan Suganya  Sabiha Hayath Basha  Manoharan Parthiban  Natesan Pazhanivel  Angappan Mangala Gowri 《Reproduction in domestic animals》2020,55(8):931-942

In swine, even though the pregnant sows were with iron abundance, the inborn iron reserve of piglets was compromised. This indicates the insufficiency of molecular machinery involved in local placental iron flux. Here, we investigated the expression of iron regulatory proteins like hepcidin and ferroportin and also their association with iron reserve, inflammation and oxidative stress in placenta of full-term pregnant sows (n = 6). Amplification and sequencing of placental DNA confirmed the presence of hepcidin (MN579557) and ferroportin (MN565887) sequences and their 100% identity with existing GenBank data. Real-time amplification of placental mRNA revealed significant higher expression of hepcidin (p < .05) than ferroportin. Western blot analysis of placental tissues revealed specific bands for both hepcidin (~8 kDa) and ferroportin (~62 kDa) molecules. Immunohistochemistry revealed the immunoreactivity for both proteins in the cytoplasm and membrane of trophoblastic cells of the placenta. Hepcidin and ferroportin expressions were positively associated with placental non-haem iron reserve (p < .0001; p = .033), lipid peroxidation (p = .0060; p < .0001) and reactive oxygen species level (p = .0092; p = .0292). Hepcidin expression was positively associated with interleukin – 6 (p = .0002) and interferon gamma (p < .0001) expressions but ferroportin expression was negatively associated with interleukin-6 (p = .0005), interleukin-1β (p = .0226) and interferon gamma (p = .0059) expressions. This indicates hepcidin and ferroportin may have a role in controlling the local placental iron flux by acting as a molecular bridge between iron trafficking and inflammation.  相似文献   

Assessment of infectious salmon anaemia virus prevalence for different groups of farmed Atlantic salmon, Salmo salar L., in New Brunswick     

McClure CA  Hammell KL  Dohoo IR  Nerette P  Hawkins LJ 《Journal of fish diseases》2004,27(7):375-383

Abstract Infectious salmon anaemia (ISA) virus (ISAV) has been causing disease in New Brunswick since 1996. As a control measure, all fish in an outbreak cage are killed. The objective of this study was to compare ISAV prevalence in cages experiencing an outbreak with healthy cages from the same farm, neighbouring farms and distant farms. Atlantic salmon from five different groups were tested using an RT-PCR test. Groups included moribund fish from a cage experiencing an outbreak (A), healthy fish from an outbreak cage (B), healthy fish from a negative cage from a farm experiencing an outbreak in a different cage (C), healthy fish from a negative farm near an outbreak farm (D), and healthy fish sampled at a negative farm located in an area with only negative farms (E). Apparent prevalences (standard error) for the different groups (A-E) were 0.94 (+/-0.026), 0.41 (+/-0.062), 0.29 (+/-0.040), 0.08 (+/-0.037) and 0.08 (+/-0.037), respectively. All groups were significantly different (P < 0.002) from each other except for groups B and C and groups D and E. Because the prevalence of the virus was significantly higher in the outbreak cage (B) compared with other sites, early harvest of outbreak cages will remove one source of virus. However, ISA negative cages (C) that remain on the positive farm may potentially act as a viral reservoir.  相似文献   

Mortality patterns in infectious salmon anaemia virus outbreaks in New Brunswick, Canada     

Hammell KL  Dohoo IR 《Journal of fish diseases》2005,28(11):639-650

Mortality levels attributed to infectious salmon anaemia viral (ISAV) infections were examined at the net pen and site level in the 1996 smolt year class in three areas of New Brunswick, Canada. The year class in this region was the first known to have potential exposure to ISAV beginning at the time of seawater transfer. There was considerable variability in mortality patterns among net pen groups of fish. Net pen outbreak definitions were based on at least seven high mortality days in which there were at least 100 per 100 000 fish per day or >5% cumulative mortality for the study period. There were 106 net pen outbreaks in a study population consisting of 218 net pens. Although the number of new cases decreased as water temperature decreased, overall mortality levels at the study sites did not decrease noticeably. The median peak daily mortality rate during outbreaks was 492 per 100 000 fish per day, with 10% of cases experiencing >5200 mortalities per 100 000 fish per day. The median duration of outbreaks in net pens for which the fish were not slaughtered during the outbreak was 33 days and the median total loss in those outbreaks was 6600 per 100 000 fish.  相似文献   

Post-haemorrhagic anaemia in sea bass,Dicentrarchus labrax (L.), caused by blood feeding of Ceratothoa oestroides (Isopoda: Cymothoidae)   总被引：1，自引：0，他引：1  

Horton T  Okamura B 《Journal of fish diseases》2003,26(7):401-406

The effects of the fish parasitic isopod, Ceratothoa oestroides (Risso), on haematological parameters of its cage-cultured sea bass host, Dicentrarchus labrax (L.), were studied. Analyses of blood parameters (cell counts, haemoglobin content and haematocrit) were carried out on parasitized and unparasitized sea bass from a fish farm in Turkey. Parasitized fish had significantly lowered erythrocyte counts, haematocrit and haemoglobin values and significantly increased leucocyte counts. Blood feeding by C. oestroides thus produces a post-haemorrhagic anaemia and the fish appear to mount an immune response to the presence of parasites.  相似文献   

An evaluation of current diagnostic tests for the detection of infectious salmon anaemia virus (ISAV) following experimental water-borne infection of Atlantic salmon,Salmo salar L     

Snow M  Raynard RS  Murray AG  Bruno DW  King JA  Grant R  Bricknell IR  Bain N  Gregory A 《Journal of fish diseases》2003,26(3):135-145

  相似文献   

Myelodysplasia, hypophosphataemia, vitamin D and iron deficiency in an alpaca.     

S L Murray  K W Lau  A Begg  K Jacobs 《Australian veterinary journal》2001,79(5):328-331

A pregnant 2-year-old alpaca was presented for evaluation of progressive weight loss, decreased appetite and lethargy that developed in winter. Haematologic and serum biochemical analyses revealed marked anaemia, leukopenia, severe hypophosphataemia and mild hypocalcaemia. Evaluation of bone marrow core biopsies and aspirates revealed an increased proportion of immature haematopoietic cells, without sufficient numbers of blast cells to be termed an acute myeloid leukaemia (AML). 1 The haematological and bone marrow findings were suggestive of myelodysplastic syndrome (MDS). The anaemia, leukopenia, lethargy and weight loss remained refractory to medical therapy and the alpaca was euthanased on humane grounds.  相似文献   

Evaluation of prognostic indicators for canine primary immune‐mediated haemolytic anaemia and application of a scoring system for the determination of prognosis          下载免费PDF全文

SS Ke  GA Anderson  SL Connolly 《Australian veterinary journal》2015,93(4):93-98

  相似文献