The interaction of infectious salmon anaemia virus (ISAV) with the blue mussel,Mytilus edulis     

Sally D. Molloy  Michael R. Pietrak  Deborah A. Bouchard  Ian Bricknell 《Aquaculture Research》2014,45(3):509-518

Integrated multi‐trophic aquaculture (IMTA) is an alternative approach to mono‐culture aquaculture that reduces environmental impacts of commercial aquaculture systems by combining the cultivation of fed species with extractive species. Shellfish play a critical role in IMTA systems by filter‐feeding particulate‐bound organic nutrients. They may also increase or decrease disease risk on farms by serving as reservoirs or barriers for important finfish pathogens such as infectious salmon anaemia virus (ISAV). This study aimed to optimize culture and molecular assays in shellfish tissues and to determine the fate of ISAV in mussels, Mytilus edulis. To determine detection limits, qRT‐PCR and culture assays in both CHSE‐ and ASK cells were optimized in ISAV‐inoculated mussel tissue homogenates. Both qRT‐PCR and culture assays performed in ASK cells had comparable detection limits of 10^2.8 TCID₅₀ mL^?1. The ISAV RNA genome was consistently detected in digestive gland tissue of ISAV‐exposed mussels. Viable ISAV was not detected in mussel tissues by culture analysis in CHSE‐ and ASK cells. The fact that qRT‐PCR analysis resulted in positive cycle threshold (CT) values that corresponded to the detectable range of ISAV in ASK culture assays suggests that little to no viable ISAV particles are present in the mussel tissues.  相似文献   

Trypanosomiasis in an Australian little red flying fox (Pteropus scapulatus)          下载免费PDF全文

JT Mackie  R Stenner  AK Gillett  A Barbosa  U Ryan  PJ Irwin 《Australian veterinary journal》2017,95(7):259-261

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A modelling approach to predict the variation of repeatability and reproducibility of a RT-PCR assay for infectious salmon anaemia virus across infection prevalences and infection stages     

Caraguel C  Stryhn H  Gagné N  Dohoo I  Hammell L 《Preventive veterinary medicine》2012,103(1):63-73

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农林院校大学生文化素质教育的现状调查及对策研究——以河北农业大学为例     

李艳玲  刘洋  刘冀  王子贤 《河北农业大学学报(农林教育版)》2012,14(1):4-7

通过梳理河北农业大学在校本科生文化素质教育现状的问卷调查结果,剖析了农林类院校大学生文化素质教育方面普遍存在的文化类课程设置不足、学生传统文化贫血严重、跨文化意识薄弱等问题。针对现状,提出加强和重视校园文化建设,加强文化类公选课课程体系建设,在中西文化对比中理性传承中国传统文化,重视培养学生的创新人格及大力开展社会实践活动等加强文化素质建设对策。  相似文献   

Molecular testing of adult Pacific salmon and trout (Oncorhynchus spp.) for several RNA viruses demonstrates widespread distribution of piscine orthoreovirus in Alaska and Washington          下载免费PDF全文

M K Purcell  R L Powers  J Evered  J Kerwin  T R Meyers  B Stewart  J R Winton 《Journal of fish diseases》2018,41(2):347-355

This research was initiated in conjunction with a systematic, multiagency surveillance effort in the United States (U.S.) in response to reported findings of infectious salmon anaemia virus (ISAV) RNA in British Columbia, Canada. In the systematic surveillance study reported in a companion paper, tissues from various salmonids taken from Washington and Alaska were surveyed for ISAV RNA using the U.S.‐approved diagnostic method, and samples were released for use in this present study only after testing negative. Here, we tested a subset of these samples for ISAV RNA with three additional published molecular assays, as well as for RNA from salmonid alphavirus (SAV), piscine myocarditis virus (PMCV) and piscine orthoreovirus (PRV). All samples (n = 2,252; 121 stock cohorts) tested negative for RNA from ISAV, PMCV, and SAV. In contrast, there were 25 stock cohorts from Washington and Alaska that had one or more individuals test positive for PRV RNA; prevalence within stocks varied and ranged from 2% to 73%. The overall prevalence of PRV RNA‐positive individuals across the study was 3.4% (77 of 2,252 fish tested). Findings of PRV RNA were most common in coho (Oncorhynchus kisutch Walbaum) and Chinook (O. tshawytscha Walbaum) salmon.  相似文献   

Comparison of alfaxalone and propofol on haematological and serum biochemical variables in cats undergoing radiotherapy with sevoflurane maintenance     

《Veterinary anaesthesia and analgesia》2023,50(2):146-156

ObjectiveTo evaluate effects of repeated alfaxalone or propofol administration on haematological and serum biochemical variables in cats undergoing radiotherapy.Study designProspective, block-randomized, clinical trial.AnimalsA group of 39 client-owned cats.MethodsAfter butorphanol (0.2 mg kg^–1) and midazolam (0.1 mg kg^–1) sedation, cats were randomly assigned to receive either alfaxalone or propofol for induction of anaesthesia and sevoflurane maintenance. Cats were anaesthetized daily with the same induction agent for 10–12 days. Complete blood counts, reticulocytes, Heinz body score and serum biochemistry were performed before the first treatment (T1), at T6, T10 and 3 weeks after the final treatment (T21). Cumulative induction agent dose for each cat at each time point was evaluated for an effect on Heinz body score. Data are shown as mean ± standard deviation; p < 0.05.ResultsAt baseline there were no significant differences in signalment or blood variables between groups. A significant decrease in haematocrit of 2.3% ± 0.77 (p = 0.02) between T1-T6 and T1-T10 [mean 4.1% (± 0.78, p < 0.0001)] was detected, with a significant increase in haematocrit of 2.1% ± 0.80 (p = 0.046) between T6-T21 and 4.0% ± 0.8 (p < 0.001) between T10-T21. Heinz body score significantly increased by 1.86 ± 0.616 (p = 0.013) between T1-T10. In the propofol group, reticulocytes increased significantly between T1-T6 [mean 23,090 μL^–1 ± 7670 (p = 0.02)] and T1-T10 [mean 27,440 μL^–1 ± 7990 (p = 0.007)]. Mean cumulative dose at T10 was 19.65 mg kg^–1 ± 5.3 and 43.4 mg kg^–1 ± 14.4 for alfaxalone and propofol, respectively, with no significant effect on Heinz body formation at any time point.Conclusions and Clinical relevanceHaematocrit decreased in both groups with recovery after 3 weeks. Repeated alfaxalone and propofol administration was not associated with marked haematological or serum biochemistry changes.  相似文献   

Pancytopenia in two horses following administration of potentiated sulfonamide antimicrobials          下载免费PDF全文

L. Cudmore  S. Jalim  M. M. Williamson  A. O. McKinnon 《Equine Veterinary Education》2015,27(10):519-523

Adverse drug reactions to potentiated sulfonamide antimicrobial drug combinations have been reported in many species, including horses. This report describes the occurrence of pancytopenia in 2 horses, one subsequent to an immune mediated reaction and the other bone marrow aplasia following the administration of trimethoprim and sulfadimidine. These cases highlight the importance of judicious use of antimicrobial agents and describe adverse drug reactions in horses receiving potentiated sulfonamide antimicrobials.  相似文献   

Presumed secondary immune‐mediated haemolytic anaemia following elapid snake envenomation and its treatment in four dogs          下载免费PDF全文

HM Ong  A Witham  K Kelers  M Boller 《Australian veterinary journal》2015,93(9):319-326

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Listeriosis in Sheep: Eperythrozoon Ovis Infection Used as a Model to Study Predisposing Factors     

Hallstein Grnstl  John vers 《Acta veterinaria Scandinavica》1980,21(4):523

Three groups of 9 months old lambs, each group consisting of 5 animals, were infected experimentally with Eperythrozoon ovis (Eo), Listeria monocytogenes (Lm) and Eo/Lm, respectively. The animals infected with Eo developed haemolytic anaemia, but otherwise no clinical symptoms were seen. The animals infected with Lm had a period with fever and reduced appetite after infection. These symptoms lasted longer and were more pronounced in the group with the dual infection (Eo/Lm). None of the lambs developed clinical meningo-encephalitis during the experiment.Group Lm developed the highest reciprocal geometrical mean titres against Lm. No titer rise was found in group Eo, while group Eo/Lm had a slight rise towards the end of the experiment. Group Eo/Lm also had the strongest delayed hypersensitivity reaction against Lm.After Eo infection, a fall in packed cell volume, haemoglobin, number of red cells, and plasma glucose and an increase in serum iron were recorded. Serum iron dropped and serum copper increased after infection with Lm.In this experiment the blood changes induced by Eo, i.e. haemolytic anaemia and acidosis, led to a prolonged state of illness in animals infected with Lm, in addition to inhibited development of antibody titres, but not to clinical meningoencephalitis.  相似文献   

An outbreak of equine infectious anaemia in Ireland during 2006: Investigation methodology,initial source of infection,diagnosis and clinical presentation,modes of transmission and spread in the Meath cluster     

S. J. MORE  I. AZNAR  D. C. BAILEY  J. F. LARKIN  D. P. LEADON  P. LENIHAN  B. FLAHERTY  U. FOGARTY  P. BRANGAN 《Equine veterinary journal》2008,40(7):706-708

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