白子菜提取物对急性痛风性关节炎大鼠模型抗炎作用的研究          下载免费PDF全文

刘昌盛  陈昉  何颖  李桂元  姚红  童娟 《勤云标准版测试》2016,36(8):26-29,81

目的 探讨白子菜提取物对急性痛风性关节炎大鼠模型抗炎作用。方法 将72只Wistar大鼠随机分为9组（每组8只）：空白组、模型组、秋水仙碱组、白子菜水提取物低、中、高剂量组、白子菜醇提取物低、中、高剂量组。造模5 h后开始连续灌药,7 d后取材,观察各组大鼠关节炎症情况,关节组织单位面积内炎症细胞计数及检测血清白介素-1β（interleukin-1 β,IL-1β）、肿瘤坏死因子-α（tumour necrosis factor-α,TNF-α）浓度。结果 中、高剂量白子菜提取物组与秋水仙碱组在足趾炎症情况显著低于模型组（P<0.05）。在淋巴细胞、中性粒细胞计数方面各药物组显著低于模型组（P<0.05）,各药物组间无明显差异（P>0.05）。秋水仙碱组TNF-α表达水平显著低于模型组（P<0.05）。秋水仙碱组及白子菜醇提取物中剂量组IL-1β表达水平显著低于模型组（P<0.05）。结论 秋水仙碱及中剂量白子菜醇提物对急性痛风性关节大鼠模型具有抗炎免疫作用。  相似文献   

类风湿关节炎治疗药物的分子作用机制研究进展     

王贵霞  张秀英 《东北农业大学学报》2009,40(3)

类风湿关节炎是慢性自体免疫性疾病,文章从作用于细胞因子及其受体、针对T淋巴细胞的清除、影响G蛋白偶联受体信号转导和Ras-丝裂原激活的蛋白激酶信号转导四个方面阐述了消炎免疫药物治疗类风湿性关节炎的分子作用机制。  相似文献   

新关节炎动物模型的研究进展     

刘明强  李培锋  关红 《中国畜牧兽医》2012,39(3):217-219

文章主要论述了近年来新关节炎动物模型的发展概况,具体包括3个方面的内容:新型的转基因关节炎模型的种类及发病机理、免疫复合物关节炎模型的种类及发病机理和关节炎发生发展过程中涉及到的相关细胞因子的研究。  相似文献   

笼养丹顶鹤病毒性关节炎的诊断和治疗     

尚丽元 《吉林林业科技》2017,46(3)

本文对莫莫格保护区三例丹顶鹤病毒性关节炎的发病特点、临床症状、病理变化、诊断及治疗与预防进行阐述,应加强饲养管理,及时接种疫苗,重在预防。  相似文献   

三妙散合舒筋散治疗早期湿热阻络证类风湿性关节炎的疗效观察          下载免费PDF全文

杨晶  袁慧慧  艾娟娟  谭娟  刘鹏 《勤云标准版测试》2017,37(7):774-777

目的 观察三妙散合舒筋散治疗早期湿热阻络证类风湿性关节炎的疗效。方法 将75例患者随机分为对照组和治疗组,对照组给予常规西药治疗,治疗组在对照组基础上给予三妙散合舒筋散治疗,观察比较2组总体疗效、临床症状、血清C反应蛋白（CRP）、红细胞沉降率（ESR）和类风湿因子（RF）变化。结果 治疗组总有效率92.10%,对照组总有效率72.97%,2组比较差异具有统计学意义（P<0.05）;治疗组的临床症状改善方面显著优于对照组（P<0.05）;治疗组的ESR、CRP和RF等血清指标较对照组明显降低,差异具有统计学意义（P<0.05）。结论 三妙散合舒筋散治疗早期湿热阻络证类风湿性关节炎疗效显著,值得临床推广应用。  相似文献   

复方穿山龙颗粒治疗类风湿性关节炎临床观察          下载免费PDF全文

李力  徐照  吉康生  吴春叶  刘静 《勤云标准版测试》2017,37(6):646-648

目的 观察复方穿山龙颗粒治疗类风湿性关节炎的临床疗效及不良反应。方法 将68例类风湿性关节炎患者随机分为治疗组（34例）和对照组（34例）,治疗组给予复方穿山龙颗粒,对照组给予来氟米特片和洛索洛芬钠片,疗程3个月。观察两组临床疗效以及血沉（ESR）、C-反应蛋白（CRP）、类风湿因子（RF）的变化。结果 治疗组临床疗效的总有效率为85.3%,ESR、CRP、RF较治疗前均明显降低（P<0.05）,与对照组比较差异无统计学意义（P>0.05）,但不良反应发生率明显低于对照组,差异有统计学意义（P<0.05）。结论 复方穿山龙颗粒治疗类风湿性关节炎具有疗效确切、不良反应少等优点,适合患者较长时间服用。  相似文献   

Successful treatment of infectious (Salmonella type III: 44) polyarthritis and osteomyelitis in a 4‐week‐old foal     

K. Close  M. Gerard  G. Davidson  M. Schramme 《Equine Veterinary Education》2011,23(3):121-126

This article describes the acute onset of infectious polyarthritis and osteomyelitis in a 4‐week‐old foal. Analysis of synovial fluid obtained from the left femoropatellar and right tarsocrural joints combined with clinical signs consisting of joint effusion and lameness yielded a diagnosis of septic arthritis. Bacterial culture of synovial fluid from the left stifle revealed Salmonella type III: 44. Rapid, sustained clinical improvement was noted following discontinuation of empirical antimicrobial therapy (potassium penicillin and amikacin sulphate) and initiation of treatment with ceftiofur and ampicillin. The importance of combining knowledge of veterinary pharmacology and microbiology so that appropriate antimicrobials may be selected with regard to the local environment in which they are to eradicate infection is emphasised. Despite frequent reference to amikacin sulphate as an effective antimicrobial for treating infections in foals caused by Salmonella, factors are discussed that explain why amikacin may not be clinically effective for treating infectious arthritis caused by Salmonella.  相似文献   

Low dietary silicon supplementation may not affect bone and cartilage in mature,sedentary horses     

Abby Pritchard  Brian D Nielsen  Cara Robison  Jane M Manfredi 《Journal of animal science》2020,98(12)

As osteoarthritis is a major cause of lameness in horses in the United States, improving collagen health prior to onset and increasing collagen turnover within affected joints could improve health- and welfare-related outcomes. Through its positive effects on bone mineral content and density and its role in increasing collagen synthesis, silicon (Si) may slow the development and progression of osteoarthritis, thereby reducing lameness. This study evaluated the hypothesis that Si supplementation would increase cartilage turnover through increased collagen degradation and formation markers, as well as bone formation markers, resulting in reduced lameness severity when compared with controls. Ten mature Standardbred geldings were assigned to either a Si-treated (SIL) or control (CON) group and group-housed on pasture for 84 d. Horses were individually fed to ensure no cross-contamination of Si other than what was present in the environment. For the duration of the study, SIL horses received a Si–collagen supplement at the rate of 0.3 g supplement/(100 kg body weight day). Serum samples were taken weekly for osteocalcin, and plasma samples were taken on days 0, 42, and 84 for plasma minerals. On days 0, 42, and 84, subjective and objective lameness exams were performed, and radiographs and synovial fluid samples were taken from reference and osteoarthritic joints. Plasma minerals were similar in both groups and were lower on day 84 than on day 0 (P < 0.05). Si supplementation, fed at the manufacturer’s recommended rate, did not improve lameness or radiographs when compared with controls, and supplemented horses did not show greater collagen degradation and/or synthesis markers in synovial fluid than controls, indicating that cartilage turnover remained unaffected. However, a minimum beneficial threshold and range for Si supplementation standardized to body weight need to be established.  相似文献   

Bortezomib attenuates LPS-induced inflammatory response of human monocytes through regulating miR-223/NLRP3 signaling pathway     

MO Xiang-tao  ZHANG Yi-shan  LI Yong-jun 《园艺学报》2000,36(8):1482-1487

AIM To investigate the effects of bortezomib (BTZ) on microRNA-223 (miR-223)/nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) signaling pathway and lipopolysaccharide (LPS)-induced inflammatory response of human monocytes. METHODS Monocytes were isolated and purified from peripheral blood of rheumatodid arthritis (RA) patients. The levels of interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) in supernatants of the monocytes were determined by ELISA, and the optimal induction time of LPS and the optimal treatment concentration of BTZ were selected according to the levels of IL-6, IL-1β and TNF-α. The monocytes were divided into control group, LPS induced group and BTZ group. The level of miR-223 in the monocytes was measured by RT-qPCR, and the protein levels of NLRP3, caspase-1, suppressor of cytokine signaling 1 (SOCS1) and SH2 domain-containing inositol phosphatase-1 (SHIP-1) in the monocytes were determined by Western blot. RESULTS The monocytes successfully isolated and purified from the peripheral blood of RA patients were spherical, evenly distributed and regular in shape.And after LPS induction for 24 h, the cells were mostly spindle-shaped and aggregated. According to the levels of IL-6, IL-1β and TNF-α, the optimal induction time of LPS was 24 h, and the optimal concentration of BTZ was 50 nmol/L. Compared with control group, the levels of miR-223, SOCS1 and SHIP-1 in LPS induction group were significantly decreased (P<0.05), and the levels of NLRP3 and caspase-1 were significantly increased (P<0.05). Compared with LPS induction group, the levels of miR-223, SOCS1 and SHIP-1 in BTZ group were significantly increased (P< 0.05), and the levels of NLRP3 and caspase-1 were significantly lowered (P<0.05). CONCLUSION Bortezomib may block the activation of miR-223/NLRP3 signaling pathway, thus reducing the secretion of inflammatory factors in LPS-induced human monocytes.  相似文献   

应用ELISA双抗体夹心法检测鸡病毒性关节炎病毒的研究     

唐雨德  翟春生  周宗安  顾志香  王元伦 《中国兽医学报》1993,(3)

本试验建立了检测鸡病毒性关节炎病毒的ELISA双抗体夹心法.取代反应、阻断试验均为阴性,与NDV、MDV和IBDV无交叉反应,对已知阳性标本的检测均为阳性;其敏感性比琼扩试验高80倍以上,这表明ELISA夹心法具有较高的特异性和敏感性.在人工感染后2～27d,关节滑膜、腱鞘和脾脏中病毒检出率为100％;还从肝脏、法氏囊和脑组织中检测到病毒.  相似文献