正交试验优化天然虾青素的提取工艺研究   总被引：2，自引：1，他引：1  

徐竞 《南方水产》2008,4(2):69-74

研究了负压空化法提取虾壳中虾青素的各项工艺条件对虾青素提取效果的影响,通过正交试验确定了负压空化法提取最佳工艺参数为提取溶剂是浓度80%的乙醇溶液,提取时间为35min,料液比为1：20,通气量为0.2m^3·h^-1。同时将负压空化法与常用破壁提取方法的提取效果和效率进行了比较,综合结果表明,负压空化法优越于其它方法,是一种能够适应工业生产的新型提取方法。  相似文献   

Astaxanthin Reduces Stemness Markers in BT20 and T47D Breast Cancer Stem Cells by Inhibiting Expression of Pontin and Mutant p53     

Yong Tae Ahn  Min Sung Kim  Youn Sook Kim  Won Gun An 《Marine drugs》2020,18(11)

Astaxanthin (AST) is a product made from marine organisms that has been used as an anti-cancer supplement. It reduces pontin expression and induces apoptosis in SKBR3, a breast cancer cell line. Using Western blotting and qRT-PCR analyses, this study revealed that in the T47D and BT20 breast cancer cell lines, AST inhibits expression of pontin and mutp53, as well as the Oct4 and Nanog cancer stem cell (CSC) stemness genes. In addition, we explored the mechanism by which AST eradicates breast cancer cells using pontin siRNAs. Pontin knockdown by pontin siRNA reduced proliferation, Oct4 and Nanog expression, colony and spheroid formation, and migration and invasion abilities in breast cancer cells. In addition, reductions in Oct4, Nanog, and mutp53 expression following rottlerin treatment confirmed the role of pontin in these cells. Therefore, pontin may play a central role in the regulation of CSC properties and in cell proliferation following AST treatment. Taken together, these findings demonstrate that AST can repress CSC stemness genes in breast cancer cells, which implies that AST therapy could be used to improve the efficacy of other anti-cancer therapies against breast cancer cells.  相似文献   

法夫酵母虾青素提取工艺的优化研究   总被引：12，自引：3，他引：12  

倪辉  何国庆  杨远帆  吴光斌  陈申如  蔡联生 《农业工程学报》2004,20(2):204-208

从破壁方法、浸提溶剂及提取条件等方面对法夫酵母虾青素提取工艺进行了优化研究。用单因子试验对破壁方法及浸提溶剂进行选择，结果表明二甲亚砜(DMSO)法是法夫酵母破壁提取虾青素的最佳方法，丙酮是理想的提取溶剂。用析因试验对破壁提取的条件进行了分析研究，结果表明破壁的温度、破壁的时间、浸提溶剂的添加量及浸提温度等都会对法夫酵母破壁提取虾青素产生显著影响，但以破壁的温度及丙酮的添加量影响最大。通过最速上升和中心组合设计试验，优化得到适宜的提取条件为：二甲亚砜加量25 mL/g(以干菌体计，下同)、破壁温度75.6℃、破壁时间20 min、丙酮添加量77.4 mL/g、浸提温度为40℃，优化后提取液中虾青素的浓度为3.145 μg/mL，比未优化时增加了27.02%。  相似文献   

Effects of dietary astaxanthin source and light manipulation on the skin colour of Australian snapper Pagrus auratus (Bloch & Schneider, 1801)     

Mark A Booth  Rebecca J Warner-Smith  Geoff L Allan  & Brett D Glencross 《Aquaculture Research》2004,35(5):458-464

Two experiments were conducted with Australian snapper Pagrus auratus (Bloch and Schneider, 1801). The first was aimed at determining the dietary level of astaxanthin that improved skin redness (CIE a^*values) of farm‐reared snapper. Farmed snapper (ca. 600 g) fed a commercial diet without carotenoids were moved to indoor tanks and fed the same diet supplemented with 0, 36 or 72 mg astaxanthin kg^?1 (unesterified form as Carophyll Pink?) for nine weeks. Skin redness (CIE a^* values) continued to decrease over time in fish fed the diet without astaxanthin. Snapper fed the diet containing 72 mg astaxanthin kg^?1 were significantly more red than fish fed the diet with 36 mg astaxanthin kg^?1 three weeks after feeding, but skin redness was similar in both groups of fish after 6 and 9 weeks. The second experiment was designed to investigate the interactive effects of dietary astaxanthin source (unesterified form as Carophyll Pink? or esterified form as NatuRose?; 60 mg astaxanthin kg^?1) and degree of shading (0%, 50% and 95% shading from incident radiation) on skin colour (CIE L^*a^*b^*) and skin and fillet astaxanthin content of farmed snapper (ca. 800 g) held in 1 m³ floating cages. After 116 days, there were no significant interactions between dietary treatment and degree of shading for L^*, a^* or b^* skin colour values or the concentration of astaxanthin in the skin. Negligible amounts of astaxanthin were recovered from fillet samples. The addition of shade covers significantly increased skin lightness (L^*), possibly by reducing the effect of melanism in the skin, but there was no difference between the lightness of fish held under either 50% or 95% shade cover (P>0.05).  相似文献   

Blood appearance, metabolic transformation and plasma transport proteins of 14C-astaxanthin in Atlantic salmon (Salmo salar L.)     

G.H. Aas  B. Bjerkeng  T. Storebakken  B. Ruyter 《Fish physiology and biochemistry》1999,21(4):325-334

The time of appearance in blood, and transport of astaxanthin, and catabolic transformation of astaxanthin to idoxanthin were investigated in Atlantic salmon (Salmo salar) that had been force-fed a single dose of ¹⁴C-astaxanthin. In addition to the LPs, a major protein, associated with radiolabeled astaxanthin was detected. The maximum level of radiolabeled carotenoids in blood was attained 30 h after administration of ¹⁴C-astaxanthin. Radioactive idoxanthin (combined 3,4-cis and 3,4- trans glycolic isomers of idoxanthin) appeared after 6 h and a stable level was obtained after 18 h. LPDP and LP, separated by ultracentrifugation, contained on average 89 and 11% of the total radioactivity in plasma, respectively. During the 168 h experiment, maximum radioactivity in LP appeared after 22 h. Separation of plasma by ultracentrifugation on a discontinuous NaCl/KBr-gradient and an iodixanol-gradient confirmed that most of the radiolabeled carotenoids were present in the HDPF that did not contain LPs (58%), whereas HDL and LDL contained 36 and 6% of the radioactivity, respectively. Of the recovered radioactivity, astaxanthin in the HDPF comprised 82%, idoxanthin 5% and unidentified compounds 12%, whereas HDL contained 78% astaxanthin, 22% idoxanthin and no unidentified compounds. Proteins from the fractions with the high density and high radioactivity (iodixanol-gradient) were separated by PAGE under non-denaturing conditions and showed a radioactive band with parallel migration length to BSA and salmon albumin. These results show that astaxanthin is rapidly converted to idoxanthin and that the majority of astaxanthin in the plasma is associated with a protein other than LPs, presumably albumin. The identity of this protein requires verification.  相似文献   

Effect of dietary bile extracts on serum response of astaxanthin in rainbow trout (Oncorhynchus mykiss): a preliminary study     

M.M. Mendes-Pinto  G. Choubert  & R. Morais 《Aquaculture Nutrition》2004,10(6):353-357

Effects of porcine bile extracts added at three different dietary concentrations 0, 10 and 20 g kg^?1 were studied on astaxanthin serum concentration in rainbow trout (mean weight 200 ± 7 g). Astaxanthin from micro‐algae Haematococcus pluvialis and synthetic astaxanthin (CAROPHYLL® pink) were incorporated in diets of rainbow trout at a rate of 100 mg astaxanthin kg^?1 of feed. Fish were hand fed twice a day. After 5 days of feeding there was a significant effect of the pigment source on the ratio (total blood astaxanthin per unit body weight to cumulative astaxanthin intake per unit body weight). Trout receiving synthetic astaxanthin showed a significantly (P < 0.05) higher ratio than trout fed algal astaxanthin. Increasing dietary bile extract did not lead to produce any effect on this ratio. The power of the statistical analysis is discussed. Therefore, the interaction (pigment source × dietary bile concentration) showed no more effect.  相似文献   

虾青素合成关键酶基因bkt植物表达载体的构建 及对玉米的遗传转化     

徐静  曲延英  杨庆利  禹山林  檀琮萍  侯艳华  秦松 《中国农学通报》2006,22(8):69-69

虾青素是一种具有极强抗氧化活性的类胡萝卜素，具有广泛的应用价值。β-胡萝卜素酮化酶（Bkt）是由玉米黄素合成虾青素生物合成途径中的关键酶。采用La Taq DNA聚合酶用PCR的方法从pET-28a（+）bkt中扩增得到bkt基因，用bkt基因替换pBI221中的GUS基因形成含有CaMV 35S启动子和NOS终止子的bkt基因表达盒，然后插入植物表达载体pCAMBIA1301的多克隆位点，最终获得带有选择标记和报告基因的植物表达载体pCAMBIA1301-bkt。通过农杆菌LBA4404介导将其转化进入玉米自交系齐319，转化后的愈伤经GUS组织化学染色分析表明bkt基因已经转入玉米胚性愈伤组织  相似文献   

虾青素对日本沼虾血细胞密度及吞噬活力的影响   总被引：1，自引：0，他引：1  

谢剑华  管越强  王静波 《河北渔业》2008,(1):8-10,13

从雨生红球藻粉中提取虾青素，以60mg/kg的浓度添加到日本沼虾（Macrobrachium nipponemis）的配合饵料中，在实验室条件下饲养三周，研究虾青素对日本沼虾血淋巴密度及吞噬活力（吞噬百分比和吞噬指教）的影响。结果显示添加虾青素组的日本沼虾不论是血细胞密度还是吞噬活力都显著高于对照组，表明虾膏素可显著提高日本沼虾的免疫力。  相似文献   

Astaxanthin Activates Nuclear Factor Erythroid-Related Factor 2 and the Antioxidant Responsive Element (Nrf2-ARE) Pathway in the Brain after Subarachnoid Hemorrhage in Rats and Attenuates Early Brain Injury     

Qi Wu  Xiang-Sheng Zhang  Han-Dong Wang  Xin Zhang  Qing Yu  Wei Li  Meng-Liang Zhou  Xiao-Liang Wang 《Marine drugs》2014,12(12):6125-6141

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Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia)     

Konstantin Chekanov  Elena Lobakova  Irina Selyakh  Larisa Semenova  Roman Sidorov  Alexei Solovchenko 《Marine drugs》2014,12(8):4504-4520

We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no adverse effect on the growth of the green BM1 cells. Under stressful conditions (high light, nitrogen and phosphorus deprivation), green vegetative cells of H. pluvialis BM1 grown in BG11 medium formed non-motile palmelloid cells and, eventually, hematocysts capable of a massive accumulation of the keto-carotenoid astaxanthin with a high nutraceutical and therapeutic potential. Routinely, astaxanthin was accumulated at the level of 4% of the cell dry weight (DW), reaching, under prolonged stress, 5.5% DW. Astaxanthin was predominantly accumulated in the form of mono- and diesters of fatty acids from C16 and C18 families. The palmelloids and hematocysts were characterized by the formation of red-colored cytoplasmic lipid droplets, increasingly large in size and number. The lipid droplets tended to merge and occupied almost the entire volume of the cell at the advanced stages of stress-induced carotenogenesis. The potential application of the new strain for the production of astaxanthin is discussed in comparison with the H. pluvialis strains currently employed in microalgal biotechnology.  相似文献