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【目的】研究不同种源黄芩光合特性、采收期内黄芩根产量及品质的动态变化,为黄芩优良种源的选择及最佳采收期的确定提供理论依据。【方法】采用TPS-1便携式光合作用系统,测定6个种源(甘肃、河北、陕西、山西、山东和吉林)黄芩的光合特性;通过五点法取样,测定采收期内(8-10月)黄芩根的产量;并用HPLC法测定黄芩苷含量和单株黄芩苷产量的动态变化。【结果】不同季节、不同种源间黄芩的光合生理特性差异显著,净光合速率、蒸腾速率、水分利用效率均随月份的变化而变化,表现出对气候变化的适应性,其中陕西种源黄芩的光合能力大于其他种源。不同种源黄芩根产量之间也有一定差异,陕西、吉林种源的产量较其他种源高,这与陕西、吉林种源较高的净光合速率和水分利用效率密切相关。采收期内,河北、陕西、山西和山东种源的黄芩苷含量在09-21达到高峰后下降,而甘肃、吉林种源的黄芩苷含量从09-14就开始下降。单株黄芩苷产量以陕西种源最高,甘肃和河北种源最低,陕西种源单株黄芩苷产量在09-21是甘肃种源的3倍,是河北种源的2.4倍。【结论】在吉林省内,为获得高产优质的药材,建议引种陕西、山西种源的黄芩,并在采收期内适时采收,陕西和山西种源黄芩最佳采收时间为09-21左右,吉林当地种源黄芩的最佳采收时间为09-14左右。 相似文献
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目的 通过处方前研究,阐明黄芩苷原料药（质量分数为85.5%）的基本理化性质。方法 采用HPLC法测定黄芩苷含量,分别进行黄芩苷的平衡溶解度、油水分配系数、破坏性试验、吸湿性考察等处方前研究。结果 原料药中黄芩苷在水中的平衡溶解度为0.0326 mg/mL,较甲醇、正丁醇、乙醇等溶剂低;在pH小于5.0的磷酸盐缓冲液中几乎不溶,其平衡溶解度随着pH值的增大呈增大的趋势;黄芩苷在正辛醇/水中的油水分配系数（P值）为1.19,在正辛醇/磷酸盐缓冲液（pH 2～10）中的P值均比正辛醇/水中的低;黄芩苷不耐碱,且易被氧化;在温度25℃、RH 75%条件下吸湿性较小。结论 该研究可为黄芩苷新制剂原料与剂型的选择、处方设计及工艺优化等提供实验依据。 相似文献
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Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells 
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下载免费PDF全文 HU Ge XUE Jiu-zhou LIU Jing ZHANG Tao DONG Hong DUAN Hui-qin YANG Zuo-jun RENXiao-ming MU Xiang 《农业科学学报》2012,12(4):646-654
We studied the effect of baicalin, an extract from Radix Scutellariae (a traditional Chinese medicine) in inducing mouse pulmonary microvascular endothelial cells (MPMVECs) to produce interferons (IFNs). MPMVECs were cultured in vitro in the presence of different concentrations of baicalin (10, 20, and 30 μg mL-1), and the cells and the culture media were harvested at various time intervals. The proteins and mRNA levels (relative to β-actin) of IFN-α/β and IFN-γ were analyzed by RT-PCR and enzyme-linked immunosorbent assay (ELISA). It was observed that baicalin substantially up-regulated the expression of IFN-α/β and IFN-γ. In all baicalin-treated groups, the relative levels of IFN-α/β and IFN-γ mRNAs peaked after 12 h of culturing, and IFN-α/β and IFN-γ proteins peaked after 24 h of culturing. These results suggest that baicalin can effectively induce the expression of IFNs in pulmonary microvascular endothelial cells, and thus potentially act as an antiviral compound. This study may provide background information for developing new antiviral drugs based on baicalin. 相似文献
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ZHANG Tao HUANG Hui-ling ZHANG Shuang LU Ping HU Ge SUO Zhan-wei JIANG Dai-xun MU Xiang 《中国农业科学(英文版)》2009,8(9):1143-1147
To explore the relationship between the heat-clearing and detoxicating functions and the bacteriostatic actions of berberine hydrochloride （Ber. H）, chlorogenic acid （Chlo. A）, and baicalin （Bai）, their concentrations in rabbit body fluid were compared with their minimal inhibitory concentrations （MICs）. Their concentrations in rabbit blood and tissue fluid were determined by reversed-phase high performance liquid chromatography, and their MICs to Escherichia coli were determined by tube dilution method. The results showed that the peak concentrations of Ber. H, Chlo. A, and Bai in rabbit blood were 3.2, 5.03, and 7.63 μg mL^-1, and in rabbit tissue fluid were 0.12, 0.11, and 0.12 μg mL^-1, respectively. Their MICs to E. coli were, respectively, 1.0×10^3, 3.75 × 10^3, and 6.75 ×10^3μg mL^-1, which were far higher than the concentrations in rabbit body fluids. This study indicates that Ber. H, Chlo. A, and Bai have weak bacteriostatic actions and do not reach their effective inhibitory concentrations in rabbit body fluids, and their heat-clearing and detoxicating functions are independent on the bacteriostatic actions. 相似文献
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黄芩苷、连翘酯苷对肺微血管内皮细胞分泌IFN-α、IFN-γ的影响 总被引：1，自引：0，他引：1
为了研究黄芩苷、连翘酯苷对大鼠肺微血管内皮细胞（pulmonary microvascular endothelial cells,PMVECs）分泌IFN-α、IFN-γ的影响,本试验以体外培养的PMVECs为模型,用ELISA法检测这2种中药成分在不同浓度、不同时间点诱导PMVECs分泌IFN-α和IFN-γ的量。结果表明,黄芩苷在10 μg/mL,24 h时诱导PMVECs分泌的IFN-α和IFN-γ的量均最高,连翘酯苷分别在20 μg/mL、12 h时和5 μg/mL、24 h时诱导PMVECs分泌的IFN-α和IFN-γ的量最高。这一结果为体外筛选诱导PMVECs表达干扰素的中药成分提供了参考,为进一步研究中药成分的抗病毒机理奠定了基础。 相似文献
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Santosh Shiwakoti Dennis A. Shannon C. Wesley Wood Nirmal Joshee Agnes Rimando Kathy S. Lawrence 《Journal of plant nutrition》2016,39(9):1240-1249
Greenhouse experiments were conducted to determine the effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizer on biomass yield and flavonoid content of American skullcap (Scutellaria lateriflora). Each experiment was carried out two times and consisted of six levels of each nutrient. The regressions gave maxima for dry matter, baicalein yield, and chrysin yield at 446, 412, and 351 kg N/ha for N fertilizer, respectively. Dry matter yield exhibited linear response to P application. The yield of scutellarein, baicalin, baicalein, and chrysin increased with addition of P. The regression gave maximum for dry matter at 208 kg K/ha for K fertilizer. A linear response to K fertilization was observed for scutellarein concentration. P application had the greatest effect on the flavonoids analyzed, whereas K had least, which may be attributed in part to the presence of K in the fritted clay medium. 相似文献
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本文着重研究了不同植物生长调节剂和碳源对黄芩愈伤组织生长及黄芩素、黄芩苷合成的影响。结果表明．适宜浓度的IAA、NAA、6-BA、2,4-D和蔗糖、葡糖糖、麦芽糖均促进了黄芩细胞的生长。4种植物生长调节剂中IAA、NAA较显著地促进了黄芩苷、黄芩素含量的提高。碳源中仅有40g·L^-1蔗糖的添加显著提高了愈伤组织中黄芩苷含量。3种碳源对促进黄芩素含量提高的影响顺序依次是：蔗糖〉葡糖糖〉麦芽糖。若仅以黄芩苷和黄芩素含量为单独评价指标,则分别选用0．5mg·L^-1·6-BA或1．0mg·L^-1 IAA、0．2mg·L^-1或3．0mg·L^-1NAA较合适;若综合考虑黄芩愈伤组织生长、黄芩苷和黄芩素含量,则3．0mg·L^-1 NAA最为合适．此时愈伤组织干重增殖倍数为4．98,黄芩苷产量为4．81g·L^-1,黄芩素产量为2．72g·L^-1。 相似文献
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The objective of this study was to explore the antibacterial mechanism of baicalin on Escherichia coli. Based on the determination of baicalin on Escherichia coli minimum inhibitory concentration (MIC) and growth curve, the influence of baicalin on Escherichia coli cells permeability was researched by measuring the conductivity and alkaline phosphatase (AKP) content of bacterial solution. The experimental results showed that baicalin had significantly inhibitory effect on Escherichia coli, and its MIC was 6.25 mg/mL.Baicalin could significantly increase the conductivity and AKP content of Escherichia coli bacteria solution.The results suggested that baicalin on antibacterial activity was mainly due to the changes of the cell membrane permeability and the damage of the cell wall. 相似文献
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慢呼抗口服液中黄芩甙的含量测定 总被引：1，自引：0，他引：1
采用美国Waters C18(4.6mm×150mm,5μm)色谱柱分离,以甲醇-水-冰醋酸(50:49:1)为流动相,流速为1mL·min-1,检测波长270nm为条件,建立高效液相色谱法测定慢呼抗口服液中黄芩甙的含量。结果表明,此方法线性关系良好(R=0.9995);平均回收率(n=6)99.06%,RSD=1.01%。该法简便、快捷、准确,可以作为慢呼抗口服液质量控制的有效方法。 相似文献