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Adults from spider crab, Maja brachydactyla, Balss, 1922 were kept in captivity for up to 2 years. Haemolymph of ten females was extracted monthly for monitoring several metabolites and the presence of Vibrionacea bacteria. A neurotoxic marker and hepatic metabolic and digestive enzyme capacities were contrasted between wild specimens and those reared in captivity. As a result, no differences were observed in hepatosomatic index (HSI) between males and females, but captive animals presented lower HSI than the wild ones. After 2 years of confinement, no changes in quantities of haemolymph microflora and metabolites were observed and this was considered indicative of adaptation of the broodstock to confinement. Spider crab also showed a large plasticity in their hepatopancreatic digestive capabilities suggested by the adaptation to the food supplied in captivity. Moreover, captivity conditions did not seem to negatively alter their antioxidant defences, xenobiotic capacity or neuromuscular activity, as no enhanced oxidative stress damage was shown in either males or females. These results indicate that although the confinement conditions used in this study do not mimic those from the wild, they could be considered as adequate for rearing M. brachydactyla in captivity, at least with regard to the set of parameters assessed. 相似文献
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Invertebrate uptake of lipids in the vicinity of Atlantic salmon (Salmo salar) aquaculture sites in British Columbia 
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下载免费PDF全文 Samples of invertebrates, primary producers, seawater and cores were taken surrounding several aquaculture sites in coastal British Columbia, Canada. Analysis of dissolved organic carbon (DOC) showed a significant decrease (P = 0.027) over 1 km and an overall average of 290 ± 40 μM at 1 m depth. At 10 m, the decrease was not significant and DOC averaged 81 ± 3 μM. The essential fatty acid 22:6ω3 (docosahexaenoic acid, DHA) was significantly lower nearer to the farm sites for all mollusc taxa as well as for mussels alone (P < 0.01). However, mussel wet weight as well as the zooplankton fatty acid marker (ZFA; Σ 20:1ω9, 22:1ω11, 22:1ω9) and 20:1ω9 individually, decreased significantly with distance from the farms (P < 0.03). Principal components analysis showed a similar trend with DHA being higher in molluscs further from the farm. Mussels had significantly higher amounts of DHA compared with other molluscs indicating their potential in multi‐trophic, co‐culturing systems. Bacterial fatty acid markers increased in molluscs with distance from the farms; however, 18:1ω7, which is representative of methane‐oxidizing bacteria, remained higher closer to the farm. In addition, DHA proportions in mussels showed a significant breakpoint at 339 m from the farm suggesting co‐cultured mussels would benefit from placement further than 339 m from farms where DHA would be optimized. 相似文献
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Norhan E. Saleh Rania F. Ismail Alaa El‐Din H. Sayed Eman H. Zaghloul Hosam Saleh 《Aquaculture Research》2020,51(9):3506-3519
Four hundred and fifty Nile tilapia (Oreochromis niloticus), each weighing ca. 4 g, were randomly allocated into five equal groups in triplicate. The first group acts as control (A0). A1, A2, A3 and A4 groups were fed on rations supplemented with diatom, Amphora coffeaeformis, at 2.5, 5.0, 7.5 and 10%, respectively, for 63 days. Assessment of fish growth, biochemical composition, erythrocyte alterations, gonadal maturation, serum biomarkers, and liver and intestinal pathogens was carried out to identify fish welfare. Fish were challenged by Aeromonas hydrophila injection for an additional 14 days; then, mortality rate and some blood parameters were monitored. Results demonstrated that Amphora sp. insignificantly impacted fish growth, but it induced significant depletion in lipid content. Blood smear investigation indicated erythrocyte alterations in A4 group compared with A0 group. Male gonadosomatic index and relative fecundity demonstrated an increment in A3 and A4 groups respectively. A boost in fish immunity of A3 group was demonstrated through serum biomarkers and pathogenic bacterial identification in liver and intestine compared with other groups. Cumulative mortality of stressed fish was 90% in the A0 group and decreased to 30% in the A3 group. Blood analysis of stressed fish indicated higher tolerance in the A3 group. In conclusion, Amphora sp. can be used as an immunostimulant for increasing disease resistance under stress conditions. 相似文献
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Tenderness is one of the most important qualities in meat. A proteomic approach is a suitable way to ensure meat tenderness. Thirty-six tenderloin samples from yak were classified as exhibiting high (n = 12) or low (n = 12) tenderness and were evaluated using label-free proteomics for the identification of the proteins and pathways most influential in tenderness variability. Between the two groups, proteomic changes were mainly caused by 33 differentially expressed proteins as displayed in reference patterns in heat maps. The expression of ENO2, SUCLG2, ETFDH, PGM1, TNNT3, TNNT1, HSDL2, GPI, ALAD, and COL1A1 proteins was very different between yak meats with high and low tenderness, and therefore, they are candidate biomarkers of yak meat tenderness. Furthermore, bioinformatics analyses revealed that the identified proteins are related to pentose phosphate, glycolysis, the citrate cycle, fatty acid metabolism, and the calcium signaling pathway. 相似文献
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Planktivorous vendace (Coregonus albula) utilise algae‐derived fatty acids for biomass increase and lipid deposition 下载免费PDF全文
下载免费PDF全文 Ursula Strandberg Minna Hiltunen Sami J. Taipale Sean Yeung Paula Kankaala 《Ecology of Freshwater Fish》2018,27(2):533-541
Spatial and seasonal variation in the abundance and composition of phytoplankton and other basal resources (bacteria, terrestrial detritus) influence the availability of essential polyunsaturated fatty acids (PUFA) for upper trophic levels in lake food webs. We studied accumulation, composition and content of fatty acids in planktivorous vendace (Coregonus albula) muscle tissue during the summer feeding period (May to late September) in six boreal lakes. Vendace muscle fatty acid content increased from ~17.7 mg/g DW to ~28.1 mg/g DW from spring to summer, corresponding to a ~59% increase in total fatty acids. PUFA accounted for 45%–65% of all fatty acids, and the most abundant fatty acid was docosahexaenoic acid (DHA, 22:6n‐3). The DHA content remained relatively constant throughout the sampling period (~7–8 mg/g DW), but the proportion of DHA decreased from ~40% in spring to ~30% in summer and autumn. The decrease in DHA proportion was due to accumulation of other fatty acids, primarily C18 PUFA (predominantly synthetised by algae) and monounsaturated fatty acids. A similar fatty acid accumulation pattern was observed in lipid storing copepods, an important prey for planktivorous vendace. The abundance of bacterial and terrestrial biomarker fatty acids in vendace muscle and prey items was low (<5% and <0.1% respectively). The increase in weight and the deposition of internal energy stores in vendace were achieved predominantly by algae‐based food chain. This has ecological implications, as vendace requires sufficient lipid stores for reproduction in autumn and survival over winter. 相似文献
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Mahak Singh Rongsennangba Talimoa Mollier Renphamo Nzanthung Patton Nungshitula Pongener Lahar Jayoti Bordoloi Rakesh Kumar Rahul Katiyar Meraj Haider Khan Dipjayoti Rajkhowa Vinay Kumar Mishra 《Reproduction in domestic animals》2023,58(1):27-38
The reactive oxygen species (ROS) which are produced during storage of boar semen are causing oxidative stress and leads to poor fertility. Also, tropical and sub-tropical weather condition adversely impacts the physicomorphological quality and fertility of boar sperm. The aim of this study was to examine the effects of feeding linseed oil to boar on its seminal attributes, sperm kinetics, biomarkers of antioxidant, fatty acid profile of seminal plasma (SP) and sperm and in vivo fertility. Six Hampshire crossbreed boars were fed with 90 ml linseed oil (LIN) whereas six Hampshire crossbreed boars were fed 90 ml canola oil (CON) for 16 weeks. Sperm quality was evaluated (60 ejaculates for each group; a total of 120 ejaculates) for motility, livability, abnormal morphology, acrosomal membrane integrity, hypo-osmotic swelling test (HOST) and sperm kinetic parameters by computer assisted semen analysis (CASA) at 0 h and at 72 h of storage at 17°C. Biomarkers of antioxidant (glutathione peroxidase; GPx, catalase; CAT, total antioxidant capacity; TAC) and malondialdehyde (MDA) were measured in SP and serum. Gas chromatography–mass spectrometry (GC–MS) was used for the estimation of fatty acid composition of SP and sperm. Boars fed with linseed oil had higher semen volume (p < .01) and more total sperm numbers (p < .01). Feeding linseed oil to boar enhanced seminal attributes (p < .05) at 0 h as well as at 72 h of storage. Linseed oil feeding (p < .01) improved biomarkers of antioxidants and significantly (p < .01) lowered the lipid peroxidation in serum and SP. Linseed oil feeding (p < .05) increased the proportion of alpha linolenic (ALA), arachidonic and docosahexaenoic (DHA) fatty acids in SP. The ratio of n-6 to n-3 fatty acids in sperm increased significantly (p < .01) in treatment group. Farrowing rate was significantly (p < .05) higher in treatment group. In conclusion, feeding linseed oil to boar improved the in vivo fertility, enhanced antioxidant capacity and increased the DHA content of SP and sperm. 相似文献
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Carretón E Corbera JA Juste MC Morchón R Simón F Montoya-Alonso JA 《Veterinary parasitology》2011,176(4):313-316
Cardiopulmonary biomarkers are biological parameters that can be objectively measured and quantified as indicators of pathogenic processes (heartworm disease) or as indicators of response to therapeutic intervention. To determine levels of cardiopulmonary biomarkers in canine dirofilariasis, measurements of cardiac troponin T, cardiac troponin I, myoglobin, and D-dimer concentrations were performed for dogs with and without evidence of adult heartworm infection. The results showed that levels of cardiac troponin T were undetectable in all dogs studied while levels of cardiac troponin I were higher in dogs infected with Dirofilaria immitis. In healthy dogs, levels of myoglobin and D-dimer were below detection limits of the instrument and were significantly higher in heartworm-infected dogs, notably in microfilaremic dogs. The results suggest the possibility of using troponin I and myoglobin as markers for cardiac damage and the D-dimer as a supportive tool for a diagnosis of pulmonary thromboembolism in dogs with cardiopulmonary dirofilariasis. 相似文献