CTX-M-Type Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from Cases of Bovine Mastitis in Japan     

Nobukazu SAISHU  Hiroichi OZAKI  Toshiyuki MURASE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1153-1156

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Improvement of Preimplantation Development of In Vitro-Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium     

Nobutada SAKAGAMI  Osamu NISHINO  Satoshi ADACHI  Hidenobu UMEKI  Hiroko UCHIYAMA  Kyoko ICHIKAWA  Kazuhisa TAKESHITA  Etsushi KANEKO  Kiyoshi AKIYAMA  Shuji KOBAYASHI  Hiromichi TAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1403-1405

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Estimation of the Optimal Timing of Fertilization for Embryo Development of In Vitro-Matured Bovine Oocytes Based on the Times of Nuclear Maturation and Sperm Penetration     

Keisuke KOYAMA  Sung-Sik KANG  Weiping HUANG  Yojiro YANAGAWA  Yoshiyuki TAKAHASHI  Masashi NAGANO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):653-659

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Effect of bovine colostrum intake on growth,reproductive parameters and survival in red kids     

H. Abdou  H. Marichatou  J.‐F. Beckers  I. Dufrasne  M. Issa  J.‐L. Hornick 《Journal of animal physiology and animal nutrition》2014,98(5):845-852

The aim of this study is to evaluate the efficacy of frozen Azawak colostrum supplementation on body weight (BW), average daily gain (ADG), reproductive parameters (mean age at first parturition, fertility, fecundity, prolificacy) and mortality rate among red kids. The study was conducted at the goat farm secondary centre of Maradi in Niger from September 2010 to September 2011. The control animals (n = 20) were left with their mother, while the treatment animals (n = 20) received in addition 50 ml/animal/day of bovine colostrum at birth and 15 ml/animal/day from d2 to d15. Weight was measured weekly from birth to d365. Mortalities were also recorded over the same period. For reproductive parameters, observations began at weaning (d197). Growth rate was higher (p < 0.001) in supplemented animal, and the treatment effects on ADG were observed up to 150 day after the end of supplementation. A similar long‐lasting trend was also observed in relation to the mortality rate (25% for ColG vs. 55% for ConG; p = 0.05). The age at first kidding tended to be lower in the treated group (13.8 ± 0.7 vs. 14.1 ± 0.8 month; p < 0.1). In conclusion, mild bovine colostrum supplementation induces a long‐lasting positive impact on growth rate and to a lower extent on reproduction parameters and survival rate.  相似文献   

牛结核γ-干扰素ELISA检测方法在检疫工作中的应用     

周培校  李静  张鲁安  李杰  曹瑞  张喜悦  李岩 《中国动物检疫》2014,31(1):67-71

为评价牛γ-干扰素ELISA检测方法检测牛结核的效果及国产试剂盒的检测效果,本试验首先将国产试剂盒与Prionics试剂盒对42份相对阳性的样品和105份相对阴性的样品进行对比研究。然后对5个规模化牛场的3000头奶牛首先进行国产单纯结核菌素颈部皮内变态反应试验,3天后选取皮内变态反应阳性和可疑及部分阴性牛共418头,进行牛γ-干扰素试验。结果国产试剂盒对阳性和阴性样品的检测敏感性和特异性分别为95.2%和100%,与Priobics试剂盒的符合率为99.3%。表明国产试剂盒与进口试剂盒的检测能力一致,牛γ-干扰素检测方法准确可靠。5个牛场的3000头奶牛单纯结核菌素颈部皮内变态反应试验阳性为138头,可疑105头。γ-干扰素试验对418头奶牛的检测,其中阳性74头,与颈部皮内变态反应（可疑牛暂时视为阴性）的符合率为60.5%。  相似文献   

牛细小病毒病毒样颗粒的组装及其免疫原性评价     

张越  常继涛  于力 《中国预防兽医学报》2014,36(12)

为研究牛细小病毒(BPV)VP2衣壳蛋白自组装病毒样颗粒(VLPs)以及VLPs的免疫原性.本研究以BPV-1型C7-5中国分离株的基因组序列为模板扩增VP2蛋白的编码基因,将其克隆至腺病毒穿梭载体pShuttle-CMV中,利用E.coliB J5183内同源重组将VP2基因插入腺病毒骨架质粒pAdEasy-1中,获得携带BPVVP2基因的重组腺病毒质粒(pAd-BPV-VP2).该重组质粒经Pac Ⅰ线性化后转染Ad293细胞,获得重组腺病毒rAd-BPV-VP2,第8代时滴度为107.25 TCID50/mL.间接免疫荧光、westemblot以及电镜观察结果显示,BPV VP2蛋白可以在腺病毒系统中稳定表达,并有效组装VLPs.将rAd-BPV-VP2肌肉注射免疫BALB/c小鼠,结果显示,针对BPV的血清IgG和中和(VN)抗体分别于加强免疫后2周和8周达最高水平,高峰抗体可持续至15周,VN抗体的最高滴度可达1:4 000.此外,与对照小鼠血清相比,免疫小鼠血清中的IL-2、IL-4及IFN-γ含量显著升高(p＜0.05).结果表明,rAd-BPV-VP2免疫小鼠后可以诱导机体产生针对BPV的特异性体液和细胞免疫.本研究为BPV的免疫预防及载体的开发奠定了基础.  相似文献   

Acute Escherichia coli Mastitis in Dairy Cattle: Diagnostic Parameters Associated with Poor Prognosis     

Seiichi HAGIWARA  Kouichiro MORI  Hiroyuki OKADA  Shin OIKAWA  Hajime NAGAHATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1431-1436

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Evaluation of feeding spray-dried bovine plasma protein on production performance of laying hens exposed to high ambient temperatures     

《The Journal of Applied Poultry Research》2014,23(3):393-402

The purpose of this study was to evaluate feeding 2 levels of spray-dried bovine plasma protein (SDP) on production performance of laying hens subjected to high ambient temperatures. Two groups of 96 Hy-Line W-98 hens (38 wk of age) were housed in each of 2 environmentally controlled chambers. At 40 wk of age, all hens were fed 3 diet treatments consisting of (1) a control diet (0% SDP); (2) the control diet supplemented with 0.75% SDP; and (3) the control diet supplemented with 1.50% SDP. Hens in each chamber (8 cages of 4 hens per cage) were ad libitum fed 1 of each diet for 5 wk. The heat stress (HS) chamber was maintained at 21°C (wk 1), 29°C (wk 2), and 35°C (wk 3 to 5). The thermoneutral chamber was maintained at 21°C during wk 1 to 5. A significant main effect of week was observed for hens maintained in the HS chamber for egg production, egg weight, egg mass, and feed consumption, which resulted in acute heat stress causing a reduction in these parameters. Hens fed the 1.50% SDP diet in the HS chamber produced greater (P < 0.05) egg mass on average than hens fed the control or 0.75% SDP diet (wk 1 to 5). During the second week of acute HS (wk 4), hens fed the control and 1.50% SDP diets had greater (P < 0.05) egg production than those fed the 0.75% SDP diet. During wk 5, hens in the HS chamber that were fed the 1.50% SDP diet produced more (P < 0.05) eggs than those fed the control diet. Therefore, based on the results of this study, acute HS negatively affected short-term production performance. In addition, feeding hens an SDP-supplemented diet may have a slight positive effect on production performance when maintained in acute HS conditions.  相似文献   

High‐level exogenous trans10, cis12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells     

Hongfang Wang  Hongyun Liu  Jianxin Liu  Ke Zhao  Chong Wang  Weiren Yang 《Animal Science Journal》2014,85(7):744-750

Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75–150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P < 0.05), but did not have inhibiting action on cell proliferation (P > 0.05). Treatment with 150 μmol/L trans10, cis12 CLA for 48 h resulted in a 17.1% reduction (P < 0.0001) of medium chain fatty acids (MCFA, C14 < C < C16), a 26.5% reduction (P < 0.0001) of unsaturated fatty acids (UFA) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A (acetylCoA) carboxylase (ACC) (P = 0.046), fatty acid synthase (FAS) (P = 0.017) and stearoylCoA desaturase1 (SCD1) (P = 0.002). Another finding was that trans10, cis12 CLA elevated expression of diacylglycerol acyltransferase2 (DGAT2) (P = 0.020) and long chain acylCoA synthetases (ACSL) (P = 0.032). In conclusion, higher trans10, cis12 CLA, not low trans10, cis12 CLA, inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS, ACC, SCD1, DGAT2 and ACSL.  相似文献   

Bispectral index analysis during cardiac arrest and cardiopulmonary resuscitation in a propofol‐anesthetized calf     

Francesco Aprea VMD  DECVAA  MRCVS  Olga Martin‐Jurado Dr Vet Med  Simon Jenni Dr Vet Med  Martina Mosing Dr Vet Med  DECVAA 《Journal of Veterinary Emergency and Critical Care》2014,24(2):221-225

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