miRNA-7 inhibits proliferation of human nasopharyngeal 5-8F carcinoma cells via EGFR/PI3K/Akt pathway     

SUN Dong-xun  HUANG Dong-dong  JIN Qiao-zhi  CHEN Wu-bing  CAI Zhi-yi 《园艺学报》2014,30(10):1807-1812

AIM:To investigate the relationship of microRNA-7 (miRNA-7) over-expression and epidermal growth factor receptor (EGFR)/phosphatidylinositol kinase-3 (PI3K)/protein kinase B (PKB, also called Akt) pathway in human nasopharyngeal carcinoma 5-8F cells. METHODS:The 5-8F cells were transfected with miRNA-7 mimics (carrying by Lipofectamine 2000). The expression of miRNA-7 was detected by real-time PCR. The cell proliferation was analyzed by CCK-8 assay. The cell colony-forming capability was determined by cell colony formation test. The expression of EGFR/PI3K/Akt at mRNA and protein levels was examined by real-time PCR and Western blotting. RESULTS:The expression level of miRNA-7 was significantly increased in 5-8F cells compared with negative control (NC) group and control group (P<0.01). The proliferation of NPC 5-8F cells was decreased extremely after tansfected with the miRNA-7 mimics (P<0.01), so did the result of the cell colony-formation test. The expression of EGFR/PI3K/Akt at mRNA and protein levels was significantly down-regulated compared with NC group and control group (P<0.01). CONCLUSION: Over-expression of miRNA-7 significantly inhibits the proliferation and colony-forming ability of nasopharyngeal carcinoma 5-8F cells by down-regulation of EGFR/PI3K/Akt pathway.  相似文献   

Attenuated Salmonella carrying pGRIM-19-si-survivin co-expression plasmid inhibits growth of prostate cancer subcutaneous xenografts in vivo     

ZHAO Xiao-hui  LU Li-li  GE He  LI Bing  ZHAO Xue-jian  LIU Yan-bo 《园艺学报》2014,30(6):961-967

AIM：To explore the effects of pGRIM-19-si-survivin co-expression plasmid carried by human attenuated Salmonella on prostate cancer subcutaneous xenograft growth in nude mice. METHODS：Prostate cancer xenograft model was established in nude mice. Co-expression plasmids carried by attenuated Salmonella were introduced by intraperitoneal injection. The xenograft volumes were monitored timely. Immunohistochemical staining, RT-PCR and TUNEL assay were applied to investigate the related mechanisms that pGRIM-19-si-survivin inhibited tumor growth in vivo. RESULTS：Compared with psi-survivin and pGRIM-19 carried by attenuated Salmonella (control groups), the tumor volumes were reduced markedly in pGRIM-19-si-survivin plasmid group. The mean shrinkage rates were 2.36 and 3.02 times. pGRIM-19-si-survivin co-expression plasmid carried by attenuated Salmonella inhibited survivin expression but strengthened GRIM-19 expression obviously (P<0.05). The mRNA expression of apoptosis-related proteins such as Bcl-xL, Stat3, cyclin D1 and c-Myc was inhibited, and the vascular endothelial growth factor (VEGF) mRNA and Ki67 protein were also inhibited, but the caspase-3 mRNA expression was up-regulated (P<0.05) with significant cell apoptosis. CONCLUSION： pGRIM-19-si-survivin co-expression plasmid carried by human attenuated Salmonella inhibits the growth of prostate cancer subcutaneous xenografts in nude mice by promoting cell apoptosis and inhibiting prostatic cancer proliferation.  相似文献   

Cloning and molecular characterization of an ovarian-derived (brain-like) P450 aromatase cDNA and development of a competitive RT-PCR assay to quantify its expression in the fathead minnow (Pimephales promelas)     

S. Halm  M. Rand-Weaver  J.P. Sumpter  C.R. Tyler 《Fish physiology and biochemistry》2001,24(1):49-62

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Extrapituitary gonadotropin-releasing hormone (GnRH) binding sites in goldfish     

Hamid R. Habibi  Debananda Pati 《Fish physiology and biochemistry》1993,11(1-6):43-49

In teleosts, as in other vertebrates, the secretion of pituitary gonadotropin (GTH) is mediated by the hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH). Recent findings in teleosts indicate that GnRH receptors are not restricted to the pituitary gonadotropes and are also associated with somatotropes as well as being present in a number of other tissues. In the present study, we provide novel information on GnRH binding in a number of extrapituitary tissues in goldfish. However, we do not intend to provide full characterization of GnRH binding sites in various extrapituitary tissues in goldfish as this would clearly be outside the scope of this paper. In this study we examined GnRH binding in a number of extrapituitary tissues in goldfish and observed specific binding in ovary, testis, brain, liver and kidney. No specific GnRH binding was observed in muscle, skin, gut, gill and heart. In general, the present findings together with the results of other studies carried out in our laboratory demonstrate that mature goldfish ovary and testis contain two classes of GnRH binding sites, high affinity/low capacity and low affinity/high capacity sites with binding characteristics similar to those of the pituitary GnRH receptors. The brain of goldfish was also found to contain two classes of GnRH binding sites, a super-high affinity/low capacity and a low affinity/high capacity sites. Furthermore, study of goldfish liver and kidney demonstrated the presence of a single class of GnRH binding sites with characteristics different from those of pituitary, ovary, testis and brain. Overall, it is evident that goldfish contains a family of GnRH binding sites which can be classified into four groups based on binding affinities: 1) A class of high affinity binding sites present in the pituitary, ovary and testis, 2) a class of super high affinity sites so far only detected in the brain, 3) a class of intermediate-affinity GnRH binding sites in the liver and kidney, and 4) a class of low affinity binding sites present in all the tissues containing specific GnRH binding sites except for liver and kidney.  相似文献   

Distribution of three GnRHs in the brain and pituitary of the wild Japanese flounder Paralichthys olivaceus   总被引：1，自引：1，他引：1  

Ky Xuan Pham  Masafumi Amano  Noriko Amiya  Yutaka Kurita  Kunio Yamamori 《Fisheries Science》2006,72(1):89-94

ABSTRACT:   Wild adult maturing and immature female Japanese flounder Paralichthys olivaceus were collected in June 2004 and January 2005, respectively, to clarify a possible role of gonadotropin-releasing hormones (GnRHs) in reproduction. Levels of salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and sea bream GnRH (sbGnRH) in the brain and pituitary were examined by time-resolved fluoroimmunoassay. Three forms of GnRHs were detected in the discrete brain at various levels. In the pituitary of both maturing and immature fish, sbGnRH was abundant together with a pronounced amount of sGnRH, whereas cGnRH-II was almost below the detectable limit. In maturing fish, levels of sbGnRH were high in the telencephalon, hypothalamus and pituitary, while levels of sbGnRH of immature fish were very low in these regions. These results indicate that sbGnRH is mainly responsible for gonadotropin secretion, and that sbGnRH in the anterior part of the brain is associated with gonadal maturation in the Japanese flounder.  相似文献   

Biochemistry and physiology of a family of eel natriuretic peptides   总被引：1，自引：0，他引：1  

Yoshio Takei  Richard J. Balment 《Fish physiology and biochemistry》1993,11(1-6):183-188

Three natriuretic peptides with similar structures were isolated from eels and their amino acid sequences determined; atrial natriuretic peptide (ANP) from atria, ventricular natriuretic peptide (VNP) from ventricles, and C-type natriuretic peptide (CNP) from brains. All three hormones were circulating in eel blood, and their plasma levels invariably decreased when eels were transferred from fresh water (FW) to seawater (SW). Eel ANP and VNP inhibited drinking in FW and SW eels. Eel ANP inhibited water and Na⁺ absorption by the intestine of SW eels. The potency of these ANP effects was 2–3 orders greater than those of other hormones which are known to have similar effects. Eel ANP and VNP induced antidiuresis but not antinatriuresis in FW eels. Eel ANP increased plasma cortisol level in SW eels but not in FW eels. The antidiuretic effect and the stimulation of cortisol secretion in eels are opposite to the ANP effects reported in mammals. These data suggest that ANP plays a complex role in the eel osmoregulation.

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Résumé Trois peptides natriurétiques présentant des structures similaires ont été isolés chez l'anguille et leurs séquences en acides aminés a été déterminées; le peptide atrial natriurétique (ANP) dans l'atria, le peptide ventriculaire natriurétique (VNP) dans les ventricules et le peptide natriurétique de type C dans le cerveau. Ces trois hormones sont présents dans le sang d'anguille et leur niveau plasmatique decroit invariablement quand les anguilles sont transférées d'eau douce en eau de mer. L'ANP et le VNP d'anguille inhibent l'action de boire chez les anguilles d'eau douce et d'eau de mer. L'ANP d'anguille inhibe l'absorption d'eau et de Na⁺ par l'intestin d'anguille en eau de mer. Ces effets de l'ANP sont 2 à 3 fois plus grands que ceux observés avec d'autres hormones qui sont connues pour avoir des effets similaires. L'ANP et le VNP d'anguille induisent l'antidiurèse mais pas l'antinatriurèse chez les anguilles d'eau douce. L'ANP d'anguille augmente chez ce poisson les niveaux de cortisol plasmatique en eau de mer mais pas en eau douce. L'effet antidiurétique et la stimulation de la sécrétion de cortisol chez l'anguille sont contraire aux effets de l'ANP observés chez les mammifères. Ces résultats suggèrent que l'ANP joue un role complexe dans l'osmorégulation de l'anguille.

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COMPUTED TOMOGRAPHY OF THE LLAMA HEAD: TECHNIQUE AND NORMAL ANATOMY     

John T.  Hathcock  DVM  MS  D. G. Pugh  DVM  MS  Robert E.  Cartee  DVM  MS  Linda  Hammond  DVM 《Veterinary radiology & ultrasound》1995,36(4):290-296

Computed tomography was performed on the head of 6 normal adult llamas. The animals were under general anesthesia and positioned in dorsal recumbency on the scanning table. The area scanned was from the external occipital protuberance to the rostral portion of the nasal passage, and the images are presented in both a bone window and a soft tissue window to allow evaluation and identification of the anatomy of the head. Computed tomography of the llama head can be accomplished by most computed tomography scanners utilizing a technique similar to that used in small animals with minor modification of the scanning table.  相似文献   

Activities of mesencephalic nucleus neurons during fictive swimming of the carp Cyprinus carpio     

Yoshihiko  BABA  Yasuyuki  KAKE  Masayuki  YOSHIDA  Kazumasa  UEMATSU 《Fisheries Science》2003,69(3):581-588

ABSTRACT:   Fish swim by undulatory contractions of the axial trunk musculature. It has been presumed that a descending signal from the brainstem activates central pattern generators in the spinal cord to make the swimming rhythm. In the carp the electrical or chemical stimulation of a mesencephalic nucleus, the nucleus fasciculi longitudinalis medialis (Nflm), could initiate swimming. However, it has not been established what signals originate from the Nflm in order to make the fish swim. The activity of the Nflm neurons was therefore examined electrophysiologically during fictive swimming in paralyzed carp. Three types of neuronal activities closely related to the swimming rhythms were obtained. The first type was a continuous tonic firing throughout an episode of fictive swimming. Neurons involved in this tonic activity may project to the spinal cord and contribute to the activation of spinal neurons to initiate fish swimming. The second type was accompanied by continual phasic firings occurring in rhythm with the activity of spinal motoneurons. Supposing that the swimming rhythm originates in the spinal cord, the synchronous activity in the brain neurons may suggest that in the nucleus there is a relay neuron conveying the rhythm information from the cord to other neurons there or in the brain. The third type exhibited reduced firing rates during fictive swimming. It is possible that the neurons engaged in this activity may be inhibitory and suppress the activities of other neurons in the nucleus and spinal cord during rest or during decelerating swimming.  相似文献   

QUANTIFICATION OF CEREBRAL VENTRICULAR VOLUME IN ENGLISH BULLDOGS     

Charles H.  Vite  DVM  Erik K.  Insko  PhD  MD  Helena M.  Schotland  MD  Karen  Panckeri  BA  Joan C.  Hendricks  PhD  VMD 《Veterinary radiology & ultrasound》1997,38(6):437-443

Quantitative measurement of cerebral ventricle volume of eight English bulldogs was performed using magnetic resonance (MR) imaging. The mean ventricular volume was 14.8 ml. with a range of 8.6 m1.-38.1 ml. The mean ventricular volume of two beagles was 2.2 ml with a range of 0.7 m1.3.7 ml. The percent of intracranial volume occupied by ventricle was found to be significantly larger in bulldogs (14.0%; S.D. = 7.9%) than in beagles (Range = 1.048%). The relationship between the percent of intracranial volume occupied by ventricle and measurements of body weight, age, sex, and various measures of skull anatomy of the bulldog was also determined. The relationship between ventricular volume and neurologic dysfunction was examined. There was a possible trend between high percent of intracranial volume occupied by ventricle and low body weight. This study will serve as a pilot study for examining the relationship between ventricular volume and neurologic disease in bulldogs.  相似文献   

Effect of long non-coding RNA MEG3 on invasion and migration of colorectal cancer cells     

ZHU Dong-liang  YIN Xiao-ping  WANG Fang-yuan 《园艺学报》2015,31(2):296-300

AIM: To investigate the expression of long non-coding RNA maternally expressed gene 3(MEG3) in colorectal cancer(CRC) cells, and to observe the effect of MEG3 on the invasion and migration of CRC cells. METHODS: The levels of MEG3 in human normal colon cell NCM460 and CRC cells SW48 and LoVo were detected by real- time PCR. MEG3 was over-expressed by plasmid transfection, and the effects of MEG3 on the invasion and migration of SW48 and LoVo cells were analyzed by Transwell assay and wound healing assay. The expression of matrix metalloproteinase(MMP) family proteins was determined by Western blotting. RESULTS: The level of MEG3 was down-regulated in CRC cells compared with normal colon cell NCM460. The invasion and migration of CRC cells were reduced after MEG3 over-expression. Transwell invasion and migration assays showed that the numbers of transmembrane SW48 and LoVo cells were smaller in MEG3 over-expression group than control group(CONCLUSION: The expression of MEG3 is down-regulated in CRC cells. Over-expression of MEG3 inhibits the invasion and migration of CRC cells. TIMP-2, MMP-2 and MMP-9 might play an important role in this regulation.  相似文献