A method to test wheat leaves for their reactions to inoculation with Septoria species     

E. Ubels 《European journal of plant pathology / European Foundation for Plant Pathology》1979,85(3):143-150

The construction and application is described of a polystyrol humidity box in which wheat leaves, while continuing to function as parts of living plants, can be tested for their reactions toSeptoria spp. in an atmosphere nearly saturated with water, as is required for successful infection. The method is simple, accurate ans inexpensive.Samenvatting Voor dit doel is een z.g. vochtdoos geconstrueerd (Fig. 1A). Het te toetsen blad wordt daar doorheen geleid en in de doos geïnoculeerd met een druppel conidiën-suspensie van de schimmel. Onder het blad staat wat water in de doos (Fig. 1B). Na afsluiting ontstaat in de doos de hoge luchtvochtigheid van bijna 100% die nodig is voor de infectie. Dit wordt op deze wijze eenvoudig en goedkoop gerealiseerd. De bladeren die zo worden getoetst blijven onbeschadigd functioneren aan de plant. Na enkele dagen kan de doos worden geopend en kan de symptoom-ontwikkeling worden afgewacht en gevolgd (Fig. 3A, 3B en 4). De methode leent zich voor nauwkeurig werk en vereist zeer weinig infectiemateriaal. De Technische en Fysische Dienst voor de Landbouw (TFDL), Wageningen, ontwierp en construeerde een statief voor het gebruik van de vochtdozen in serie (Fig. 2).  相似文献   

Preliminary results of using in vitro axillary and adventitious buds in mutation breeding of Chinese gooseberry     

Xiao-Shan Shen  Jue-Zhen Wan  Wei-Yi Luo  Xiao-Ling Ding 《Euphytica》1990,49(1):77-82

Summary Aseptically cultured shoots of Chinese gooseberry exhibited growth disorder and morphological aberrances, and some died after being exposed to sufficient gamma-ray irradiation. The death rate was dose dependant and the LD₅₀ was 80–90 Gy and 50–60 Gy respectively for cv. Hayward and clone 4. All petiole explants irradiated with gamma-ray could form calli as the control, but the rate of differentiation of adventitious shoots of the petiole explants decreased and was dependant on dose. Sensitivity of the shoot or petiole explants to gamma-ray irradiation varied with species. Gamma-ray irradiation did not deter either the 2-node segments from producing axillary shoots M₁, M₂, and M₃ or the advantitious shoots originating in the petiole explants and the M₃ shoots from forming advantitious roots. Therefore, using aseptically cultured axillary or adventitious buds for mutation breeding of Chinese gooseberry is feasible. A bacterium surviving in the explants lessened the efficiency of these two in vitro techniques in mutation breeding of Chinese gooseberry.Abbreviations IAA 3-indole acetic acid - IBA -indole butyric acid - MS Murashige & Skoog (1962)  相似文献   

The effect of phosphine on the absorption and circular dichroic spectra of cytochrome c and cytochrome oxidase     

K.P. Kashi  W. Chefurka 《Pesticide biochemistry and physiology》1976,6(4):350-362

The interaction of phosphine with purified cytochrome c and cytochrome oxidase was studied by absorption and circular dichroic spectrometry. Treatment of cytochrome oxidase with phosphine yielded absorption spectra in both the visible and Soret region that were similar to that produced by dithionite. Striking changes in the circular dichroic spectra were also recorded, sugesting that a valence change of the heme iron was accompanied by a conformation change of the prosthetic group.Phosphine treatment of cytochrome c also yielded spectra similar to those produced by reduction with dithionite suggesting a valence change of the heme iron. However, both the time necessary for complete reduction and the concentration of phosphine necessary for this effect suggest that, in comparison with cytochrome oxidase, cytochrome c was relatively insensitive. Reduction of cytochrome oxidase by phosphine had only a very minor effect on the intrinsic dichroic spectrum suggesting little effect on the polypeptide chains.The persistence of a component after reduction by phosphine and the subsequent reduction of this component by dithionite suggests that phosphine primarily reduced only one comonent of the cytochrome aa₃ complex. The failure of EDTA and fluoride, which are known ligands of cytochrome a₃ to affect the reduction of cytochrome aa₃ by phosphine, as well as the occurrence of a 436-nm isosbestic point of the ferric-ferrous absorption spectra suggests that phosphine reduced primarily cytochrome a. However, it is argued that a slow reduction of cytochrome a₃ cannot be ruled out.  相似文献   

新疆退牧还草工程建设与管理的探讨     

张江玲杨刚  王旭明 《四川草原》2005,(9):41-42

草地是发展畜牧业的重要基础,退牧还草工程是恢复、更新天然草地和保护生态环境的重要手段。本文就新疆实施退牧还草工程中所出现的一些问题进行了探讨。  相似文献   

菇木年龄对香菇产量和营养成份含量的影响     

朱光权  吴学谦  汪奎宏  杜国坚  吴庆其  李海波  苏庆亮  叶剑飞 《中国食用菌》2005,24(5):23-27

用南酸枣、秃瓣杜英、马褂木、麻栎、锥栗、枫香、银荆等7个优良菇木树种的不同年龄树木木屑作为栽培基质，选用126香菇品种做供试菌株，通过香菇总产量、冬菇产量、香菇营养物质含量的对比试验，结果表明，这7种菇木林树种7年树龄木屑栽菇香菇的性能优于或不低于15年成年树龄，用南酸枣、秃瓣杜英、马褂木、麻栎、锥栗、枫香、银荆作为经营短轮伐期菇木林树种是可行的。  相似文献   

转基因苜蓿研究进展   总被引：4，自引：0，他引：4  

胡清泉  玉永雄 《中国草地》2005,27(5):58-62

就苜蓿基因工程在育种上的研究、转基因技术在苜蓿上的进展做了简要介绍。  相似文献   

剡溪山水文化旅游资源开发设想     

邱德玉 《四川畜牧兽医学院学报》2005,3(4):5-8

文化底蕴越是丰厚的旅游资源，开发价值就越高。正是众多文人贤士游剡访古，使浙东剡溪的历史、文化与自然山水完美地结合在一起，形成了绚丽多彩的剡溪山水文化。文化被认为是旅游业的灵魂，是一种潜在的旅游产品。要提高山水旅游产品的文化品位，必须将山水文化的潜在价值转化为旅游产品。本文对剡溪山水文化旅游资源的开发提出了以下设想：再现“唐诗之路”山水旅游美景，并着重于按唐诗及有关文献、传说等增添新景点，沿江、沿山、于水中进行山水、文化全面开发，形成立体式、全景式的剡中山水文化游道；可开发出文化生态、修学、科考、宗教、节庆等剡溪山水文化旅游产品。  相似文献   

双孢蘑菇性亲和性相关分子标记的初步筛选   总被引：4，自引：0，他引：4  

詹才新  凌霞芬  杨家林 《食用菌学报》2002,9(4):1-8

以传统的形态，生理生化分析和最新的DCS－PDMA性亲和性测定方法为基础， 结合群体分离分析和RAPD技术来源于同一双孢蘑菇异核体菌株的12个不育同核原生质体个体进行分析，筛选与性亲和性相关的分子标记。研究结果表明，供试的12个不育同核原生质体个体被分成两大类性亲和性类型，其中一类（A^ ）包括不育同核原生质体个体B、C、D、E、F、G、H、I、J、L，另一类（A^-）则仅仅包括不育同核原生质体个体K和M，同时筛选到一个与性亲和性相关的分子标记OPA16 1500。从而为间地利用双孢蘑菇本身特有的交配型作标记来指导杂交育种工作和进一步将性亲和性基因定位分离克隆奠定了坚实的基础。  相似文献   

以固氮树种发展食用菌专用林的建议     

刘福平 《食用菌学报》2002,9(4):33-39

许多固氮树种速生丰产，萌生能力强，叶片，木屑含氮率较高，可为食用菌栽培提供理想原料，而且固氮树种在绿花荒山，保持水土，提高地力方面作用显著，以固氮树种发展短轮伐期食用菌专用林，具有较高的经济效益和生态效益，本文介绍了银合欢，黑荆，大叶相思，银荆，桤木，南洋楹和马占相思等在食用菌栽培上的应用概况，各地可因地制宜栽培合适的固氮树种作为食用菌专用林。  相似文献   

苹果梨的育种价值   总被引：5，自引：0，他引：5  

闫忠业 《北方果树》2003,(4):1-3

该文指出,苹果梨综合性状优良,是很好的梨育种种质资源;以苹果梨及其后代为亲本已选育出30余个新品种,大大丰富了梨种质资源;苹果梨的大果、耐贮、小果心、抗寒等优良性状在后代品种中有较强的表现;以苹果梨与多倍体品种杂交后代为多倍体的比率较高。并展望了苹果梨在今后育种中的主要研究方向。  相似文献