稀土对烤烟生长和代谢的影响研究   总被引：2，自引：0，他引：2  

张晓海  刘大永 《西南大学学报(自然科学版)》1997,19(5):431-433

烤烟叶面喷施稀土表明，６００ｍｇ／Ｌ处理，移栽４５天后。烟株干物重、光合强度、三磷酸腺苷、硝酸还原酶、过氧化氢酶分别比对照提高１７．０８％，３４．４６％，３４．４１％，２４．５１％和３４．１１％；移栽８５天后，烟叶的干物重、Ｋ２Ｏ，总糖、总氮和蛋白质含量分别比对照提高４９．６２％，２８．９６％，１２．００％，１４．１３％和２５．８４％。  相似文献   

金边卫矛冷驯化期间SOD和POD同工酶及蛋白的研究     

郭惠红  宋菲  沈昕  胡青  高述民  李凤兰 《北京林业大学学报》2005,27(6):56-61

采用凝胶电泳技术比较观察了金边卫矛冷驯化期间叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）同工酶谱及总蛋白的动态变化，就其变化规律与抗寒性的关系作了探讨,并首次鉴定了叶片中SOD的类型.结果表明:冷驯化期间叶片SOD同工酶未发生谱带数目的变化，但有谱带的增强表达，其中Cu-ZnSOD的变化较FeSOD明显.POD同工酶不仅有酶带的增强表达，而且有一条新的弱酶带出现.SOD、POD同工酶谱带随着冷驯化时间的延长而增强表达，但到达一个高峰后逐渐回落到接近对照水平.这表明植株在冷驯化期间随着SOD、POD同工酶变化而形成的低温适应机制可能是提高植株抗寒力的一个重要原因.金边卫矛在冷驯化期间诱导出4种分子量分别为18.7、19.5、15.7和18 kD的酸性蛋白，脱锻炼后仍然存在，表达量基本维持在低温处理时的水平，推测它们可能是抗冻蛋白，与冷驯化后金边卫矛在冰冻温度下的抗冻性相关.同时发现，叶片中多种蛋白质的含量与未驯化苗相比都有所增加，其中分子量分别为17.7、15.8 kD的碱性蛋白B和C及分子量为15.5 kD的酸性蛋白D的增强表达趋势较为明显，这3种蛋白在脱锻炼后含量均下降到接近对照水平，推测与植物在低温条件下代谢途径的改变有关.   相似文献   

四川省高粱种质资源品质性状分析     

丁国祥  杨乾华 《西南大学学报(自然科学版)》1995,17(1):59-61

本文分析鉴定了５２３份四川高粱种质资源的蛋白质，赖氨酸及单宁含量，并讨论了品质性状的改良问题。  相似文献   

隐性乳房炎对牛乳蛋白质脂肪和乳糖含量的影响   总被引：1，自引：0，他引：1  

宋宣明  吴光权  侯大军  艾晓杰 《西南大学学报(自然科学版)》1993,(3)

对牛乳中主要营养物质含量进行了测定,并用 CMT 法检查了隐性乳房炎。结果表明,隐性乳房炎能显著地降低乳汁中蛋白质,脂肪和乳糖的含量,从而降低了牛乳的品质。  相似文献   

杨树越冬期间储藏蛋白质的动态变化规律   总被引：2，自引：0，他引：2  

于彬  郭彦青  彭方仁 《浙江林学院学报》2007,24(6):692-695

为了探索营养储藏蛋白质在杨树体内的积累机制和生理功能,采用凝胶电泳SDS-PAGE,对1年生797杨Populus deltoids×P.euramericana越冬期间皮层和根部中的蛋白质质量分数及营养储藏蛋白质组分进行了测定分析。结果表明,这些部位中的蛋白质在越冬期间质量分数逐渐增加;皮层是杨树越冬期间储藏蛋白质的主要场所。越冬期间储藏蛋白质组分变化最明显的是36,32,22,15 kD等蛋白;落叶时蛋白谱带最清晰,36和32 kD蛋白质是杨树营养储藏蛋白质的主要类型。图3参11  相似文献   

G protein signalling involved in host recognition and mycoparasitismrelated chitinase expression in Trichoderma atroviride          下载免费PDF全文

Susanne Zeilinger　 Barbara Reithner　 Kurt Brunner　 Valeria Scala　 Isabel Peiβl　 Matteo Lorito　 Robert L Mach 《浙江大学学报(农业与生命科学版)》2004,30(4):448-448

Mycoparasitic species of Trichoderma are commercially applied as biological control agents against various fungal pathogens. The mycoparasitic interaction is host specific and includes recognition, attack and subsequent penetration and killing of the host. Investigations on the underlying events revealed that Trichoderma responds to multiple signals from the host (e.g. lectins or other ligands such as low molecular weight components released from the host's cell wall) and host attack is ac…  相似文献   

Analysis of differential protein expression during the early stage of in vitro tuberization in taro     

Hongmei Du  Ming DingDongmei Tang  Danfeng Huang 《Scientia Horticulturae》2011

Tuberization is a complex and multilevel developmental process. Many important metabolic changes in the early stage of tuberization are crucial to the tuber differentiation and development. In this study, we attempted to identify proteins differentially expressed in the early stage of in vitro tuberization in taro (Colocasia esculenta var. antiquorum) by two-dimensional polyacrylamide gel electrophoresis (2-DE) and mass spectrometry (MS). Protein samples from shoot tips cultured in 8% sucrose media at 0, 2, 4, 6 and 8 d were separated with 2-DE. A total of 13 differentially expressed proteins were analyzed with MS. Four proteins via, glyceraldehyde-3-phosphate dehydrogenase, alcohol dehydrogenase, chloroplast protein synthesis elongation factor (EF-Tu), and ankyrin repeat protein HBP1 were successfully identified during in vitro tuberization of taro. This implies that important metabolic changes, including sucrose metabolism, signal transduction and cell defense, occurred in the early stage of in vitro tuberization in taro.  相似文献   

Protective role of heat-shock protein 70 in gastric mucosal lesion induced by endotoxin in cirrhotic rats with portal hypertensive gastropathy     

LAN Cheng  SUN Xiao-ning  DONG Li-wei  YUAN Su  HUANG Bai-li  WU Ke-li 《园艺学报》2011,27(9):1816-1819

AIM: To investigate the protective role of heat-shock protein 70 (HSP70) in the pathogenesis of gastric mucosal damage in cirrhotic rats with portal hypertensive gastropathy (PHG).METHODS: The rat model of liver cirrhosis with PHG was established by injection with tetrachloride.The animals were divided into normal control group, PHG group, PHG+heat treatment group, PHG+BPI21 group and PHG+endotoxin groups.The endotoxin used in the experiment was at the dose of 3 mg/kg and endotoxin antagonist BPI21 was at the dose of 2 mg/kg.HSP70 was induced by pre-treating the animals with mild whole-body heating.The levels of HSP70 and tumor necrosis factor alpha (TNF-α) in the gastric mucosa were measured by ELISA.Furthermore, the pathological changes of the gastric mucosa were observed under microscope with HE staining.RESULTS: Compared with the normal control rats, the rats in PHG group showed obvious gastric pathological lesion, decrease in HSP70 production and increase in TNF-α level in the gastric mucosa, and increased endotoxin concentration in the plasma.Compared with PHG+endotoxin group, the gastric mucosal lesion in PHG+BPI21 group was significantly attenuated, accompanied by the increase in HSP70 production and decrease in TNF-α level in the gastric mucosa.Heat treatment increased HSP70 production and decreased TNF-α concentration in the PHG rats, thus attenuating the gastric mucosal damage.CONCLUSION: HSP70 alleviates the gastric mucosal lesion induced by endotoxin in cirrhotic rats with PHG and decreases the concentration of TNF-α in gastric mucosa, indicating a protective role of HSP70 in the pathogenesis of gastric mucosal damage in PHG.  相似文献   

十字花科上的链格孢属真菌可溶性蛋白质凝胶电泳分析   总被引：2，自引：0，他引：2       下载免费PDF全文

陈伟群  张天宇 《西北农业学报》1993,2(4):53-58

从国内外收集生于十字花科植物上的3种链格孢属真菌21个菌株,进行菌体可溶性蛋白质的凝胶电泳分析.对可溶性蛋白质图谱资料的模糊聚类分析,在较高的相似性水平上将参试菌株分为3大类群,每一类群所包括的菌株与形态学的鉴定结果完全一致,即1个类群代表1个种.种间蛋白图谱差异明显,而种内菌株间基本一致.各菌株的归类关系与其寄主植物、地理来源无关.表明可溶性蛋白质凝胶电泳在Alternaria种级分类鉴定中是一个有用的工具,可以明确地将各菌株鉴定到种级水平.  相似文献   

牛GM-CSF与金黄色葡萄球菌黏附素FnBPB共表达质粒的构建与原核表达   总被引：1，自引：0，他引：1  

范鑫  郝永清  张爱荣  陈晓杰 《动物医学进展》2011,(10):79-82

采用PCR方法对牛粒细胞-巨噬细胞集落刺激因子(GM-CSF)和金黄色葡萄球菌FnBPB的D区进行特异性的扩增,并通过重叠延伸PCR扩增GM-CSF-FnBPB串联基因,构建了克隆质粒pMD19-GM-CSF-FnBPB。利用表达载体pET-32a(+)对该融合基因片段进行原核表达,SDS-PAGE分析表明,在1mmol/L IPTG诱导浓度下,在39ku处出现了与目的蛋白一致的外源蛋白带,Western blot分析表明,该蛋白具有反应原性,进而证明该融合基因成功在原核细胞中表达。  相似文献