Protein Phosphorylation and Protein Patterns in Developing Endosperms of Barley     

T. Yupsanis  P. R. Shewry 《Plant Breeding》1993,111(1):73-77

The SDS-soluble protein-fractions of barley endosperms (2 and 3 weeks after anthesis) were compared using 1-D SDS-PAGE and 2-D IEF/SDS-PAGE. Similar procedures have been followed after in vivo phosphorylation of isolated endosperm with ³²PO₄.Protein patterns were similar when 1-D SDS-PAGE was used. On the other hand, 2-D IEF/SDS-PAGE revealed several major differences, the most important being the appearance of a major protein of Mr about 40,000 in the 3-weeks old endosperm. By contrast, the patterns of phosphorylated proteins, with both methods, were relatively simple. Only two phosphorylated bands showed up in the 3-weeks old endosperm: a highly labelled band of Mr about 65,000, and a band of about 75,000. In the case of 2-weeks old endosperm, where the phosphorylation was more active, these and several others were also labelled, but the Mr 75,000 band was labelled more intensely.  相似文献   

马铃薯A病毒(PVA)运动相关蛋白质的研究进展     

吴兴泉  陈士华 《安徽农业科学》2007,35(8):2244-2245

综述了马铃薯A病毒(PVA)运动相关蛋白的结构、功能方面的研究进展.  相似文献   

Cloning and Sequencing of a 21 kDa Outer Membrane Protein Gene of <Emphasis Type="Italic">Leptospira interrogans</Emphasis> serovar Canicola strain Hond Utrecht IV     

Cheema PS  Srivastava SK  Chaudhuri P  Amutha R  Singh S  Sankar M  Thangapandian E 《Veterinary research communications》2007,31(5):521-527

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洋葱苯丙氨酸解氨酶基因AcPAL2的克隆与表达分析     

梁毅  刘小义  张洪伟  谭武平 《园艺学报》2015,42(3):505-510

利用简并PCR技术和RACE技术克隆得到了一条洋葱的苯丙氨酸解氨酶(PAL)基因全长cDNA序列。该cDNA序列全长2349 bp,编码长685个氨基酸残基的多肽序列,命名为AcPAL2。Blast分析表明该序列与虎眼万年青Galtonia saundersiae、野蕉Musa balbisiana的相似性均较高。Real-time PCR表达及花青素含量分析表明,红皮洋葱该基因表达量最大,而黄皮和白皮洋葱表达量极低;在红皮洋葱中该基因在膨大初期大量表达,并迅速降低至一定程度后趋于相对平稳表达,且与花青素的积累过程相一致。  相似文献   

Progress of ELMO family on malignant tumor invasion and metastasis     

GUO Cheng-ming  PENG Hui-yun  LUO Wen  YU Qiong-fang  GAO Dian 《园艺学报》2015,31(12):2296-2300

It is one of the main characters of malignant tumors that malignant tumor cells invade surrounding tissues and metastasize to distant tissues. Multiple factors are involved in this complicated dynamic process. Metastasis is the major factor influencing recurrence and prognosis. Therefore, it is important to explore the mechanism of invasion and metastasis for reducing recurrence rate and mortality of malignant tumors. Engulfment and cell mobility (ELMO) family is one kind of conserved protein in evolutional process. It includes 3 members, ELMO1, ELMO2 and ELMO3. The members of ELMO family play an important role in cell phagocytosis and cell migration, and they also have close correlation with malignant tumor cell invasion and metastasis. In this paper, we review the progress of the relationship between ELMO family and malignant tumor invasion and metastasis.  相似文献   

干旱处理对华北绣线菊和毛果绣线菊光合作用及相关蛋白表达的影响     

刘计璇  胡冰  仉茜  苏青  张娇  刘慧民 《园艺学报》2015,42(5):917-923

以抗旱力弱的毛果绣线菊与抗旱力强的华北绣线菊为试验材料,采用不同程度干旱处理,在测定其光合作用变化的基础上,采用蛋白质组学技术研究两种绣线菊光合作用相关蛋白表达的变化。结果表明:干旱胁迫两种绣线菊光合能力降低,光补偿点和光饱和点降低,说明利用弱光能力提高,华北绣线菊适应弱光环境的能力强于毛果绣线菊。不同水平干旱处理分别鉴定出涉及能量代谢与运输的相关蛋白20种。干旱胁迫下毛果绣线菊中卡尔文循环途径相关酶、光系统Ⅱ中多数放氧复合蛋白和捕光色素复合蛋白、参与光合磷酸化的酶发生显著上调和下调表达;华北绣线菊中参与卡尔文循环代谢相关酶、PSⅠ中反应中心蛋白和参与光合电子传递的蛋白、PSⅡ中捕光色素复合蛋白、参与光合磷酸化与氧化磷酸化的蛋白发生明显的上调和下调表达。干旱胁迫使抗旱力不同的毛果绣线菊和华北绣线菊光合作用相关蛋白均受到影响,毛果绣线菊中多是能量代谢相关蛋白发生显著变化,华北绣线菊中多是能量运输相关蛋白发生显著变化。  相似文献   

An Effect of Various Nitrogen Forms Used as Fertilizer on Lupinus albus L. Yield and Protein, Alkaloid and α-Galactosides Content     

D. Ciesio&#;ka  M. Muzquiz  C. Burbano  P. Altares  M. M. Pedrosa  W. Wysocki  W. Folkman  M. Popenda  K. Gulewicz 《Journal of Agronomy and Crop Science》2005,191(6):458-463

The influence of various forms of nitrogen supplied to the ground as N₂, NH, NO, [NH + NO], –NH₂, and Nd (N‐deficiency) on Lupinus albus L. cvs. Butan (sweet) and Bac (bitter) yield and protein, alkaloid and α‐galactoside biosynthesis in their seeds were studied. The experiments were performed in a greenhouse on perlite using, in all cases, the same dose of P, K, Mg and micronutrients (B, Zn, Mn, Cu, Mo, Fe). The Nd treatment contained only macro‐ and micronutrients without any nitrogen support. We found that different nitrogen forms used as a fertilizer had a significant influence on the development and yield structure of both lupin cultivars. It was found that the plants developed most favourably when nitrogen was supplied as N₂ and [NH + NO]. For the other forms, the anomalies like necrosis, chlorosis, leaves with small surface of assimilation were noticed. However, these observations were not always reflected by all parameters of yield structure. The forms of nitrogen had a pivotal influence on yield of generative and vegetative parts of lupin. Moreover, significant differences in protein, alkaloid and α‐galactoside content in seeds were observed.  相似文献   

Electrophoretic Characterization of Quinoa Seed Proteins     

D. J. Fairbanks    K. W. Burgener    L. R. Robison    W. R. Andersen  E. Ballon 《Plant Breeding》1990,104(3):190-195

Quinoa (Chenopodium quinoa Willd) is an important domesticated food crop of the Andean highlands with potential as an alternative crop elsewhere. Among its most attractive characteristics are the quantity and favorable amino acid balance of the seed proteins. The objective of this study was to characterize quinoa seed proteins by electrophoretic mobility, solubility fractionation, and genetic variability from a wide genetic base. Electrophoretic profiles of denatured albumin, globulin, prolamin, and glutelin solubility fractions demonstrated that quinoa seed polypeptides could be classified as either albumin or globulin with most predominant polypeptides in the globulin fraction. Insignificant amounts of protein were present in the prolamin fraction and all polypeptides in the glutelin fraction had identical electrophoretic mobilities to albumins and globulins. Three globulin polypeptides of 34.3, 35.6, and 36.2 kilodaltons in size were highly variable within and among the accessions examined and appear to be coded by at least two loci. Two-dimensional peptide mapping revealed that these three polypeptides were homologous. These highly variable markers could be used for identification and classification of germplasm and elucidation of systematics and genetic variability within the quinoa germplasm pool. All other major polypeptides were electrophoretically invariant among the accessions examined.  相似文献   

Relationship Between Gluten Strength and Glutenin Proteins in Durum Wheat Cultivars   总被引：6，自引：0，他引：6  

J. M. Carrillo    J. F. Vazquez  J. Orkellana 《Plant Breeding》1990,104(4):325-333

An electrophoretic study of gliadin and glutenin proteins, mainly low-molecular-weight (LMW) Glutenin Subunits, was undertaken to investigate possible assoeiations between these proteins and gluten strength. Thirty-eight durum wheat cultivars having different origins and currently grown in Spam were analysed. Different electrophoretic methods were used to analyse the seed storage proteins. Protein grain content was estimated and gluten strength was measured by the SDS-sedimentation test. New patterns for LMW glutenins were observed. Besides the known patterns of LMW-1, associated with γ-gliadin 42, and LMW-2 associated with γ-gliadin 45, six cultivars had LMW-2^? associated with γ-gliadin 43, one cultivar showed LMW-2* associated with γ-gliadin 44, and another cultivar, null for γ-42 and γ-45, had LMW-1^?. Significant differences for gluten strength were found among groups of cultivars with different LMW patterns. High molecular weight glutenins were found in general to be poor indicators of viscoelastic properties, although band 20 showed a negative influence on quality. The results are discussed in relation to development ol cultivars with good gluten strength.  相似文献   

Identification of cultivars of pasture legumes (Centrosema macrocarpum,C. pubescens and C. sp.n.) by acid polyacrylamide gel electrophoresis of cotyledons storage proteins     

A. Hussain  H. Ramirez  W. M. Roca  W. Bushuk 《Euphytica》1988,39(2):105-107

Summary An electrophoretic procedure was developed for storage proteins which can discriminate cultivars of forage legumes, Centrosema macrocarpum, C. pubescens and C. sp.n. Proteins extracted from cotyledons were separated in 10% polyacrylamide gel containing 10% sucrose. Electrophoretic patterns are presented to illustrate the results that can be obtained with the procedure described. Cultivars of all three species were distinguishable based upon the variation in their acid soluble seed proteins.Joint publiccation of the Department of Food Science, University of Manitoba (No 101) and Centro Internacional de Agricultura Tropical-CIAT.  相似文献