全文获取类型
收费全文 | 510篇 |
免费 | 41篇 |
国内免费 | 68篇 |
专业分类
林业 | 6篇 |
农学 | 34篇 |
基础科学 | 6篇 |
11篇 | |
综合类 | 235篇 |
农作物 | 106篇 |
水产渔业 | 1篇 |
畜牧兽医 | 102篇 |
园艺 | 2篇 |
植物保护 | 116篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 16篇 |
2021年 | 16篇 |
2020年 | 26篇 |
2019年 | 15篇 |
2018年 | 18篇 |
2017年 | 19篇 |
2016年 | 27篇 |
2015年 | 24篇 |
2014年 | 48篇 |
2013年 | 41篇 |
2012年 | 36篇 |
2011年 | 29篇 |
2010年 | 33篇 |
2009年 | 29篇 |
2008年 | 25篇 |
2007年 | 14篇 |
2006年 | 29篇 |
2005年 | 17篇 |
2004年 | 13篇 |
2003年 | 17篇 |
2002年 | 14篇 |
2001年 | 9篇 |
2000年 | 17篇 |
1999年 | 12篇 |
1998年 | 10篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1995年 | 12篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 5篇 |
1987年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有619条查询结果,搜索用时 31 毫秒
131.
132.
在直播稻上进行30%爱苗乳油防治纹枯病、稻曲病、紫秆病试验,结果表明:用30%爱苗乳油1500倍液在水稻破口期和齐穗期用药2次效果最好,稻曲病防效达83%以上,紫秆病防效达70%以上,纹枯病防效达77%以上,且具有一定的增产作用. 相似文献
133.
A stable Leifsonia xyli subsp. xyli GFP‐tagged strain reveals a new colonization niche in sugarcane tissues 下载免费PDF全文
M. C. Quecine T. M. Silva G. Carvalho S. Saito M. Mondin N. S. Teixeira‐Silva L. E. A. Camargo C. B. Monteiro‐Vitorello 《Plant pathology》2016,65(1):154-162
Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is one of the most economically important diseases of sugarcane worldwide. Because knowledge on the interaction of Lxx with its host at the microscopic level is limited, the development of tools to monitor Lxx during the colonization process could shed new light on the processes that control disease development. In this investigation, a transformation protocol was optimized and a mutant Lxx strain engineered that stably expressed the gfp gene in sugarcane tissues. In vitro, the growth of the mutant did not differ from that of the wild type. Also, plants inoculated with both strains showed comparable growth and development when analysed 180 days after inoculation (dai). Fluorescence microscopy of roots, stalks, meristems and leaf tissues of Lxx‐GFP‐inoculated plants was performed at 180 dai. In the leaves, Lxx‐tagged cells were observed within the xylem vessels as has been described before but, in addition, they were found in a new niche within the host tissues, in the mesophyll and in the bundle sheath cells surrounding the vascular system. This finding indicates that Lxx is able to move from the xylem to the parenchyma of the leaf cells. This first report of an Lxx mutant expressing a heterologous gene revealed that colonization of sugarcane by this pathogen is not limited to the xylem vessels as commonly reported. 相似文献
134.
135.
玉米抵御鞘腐病菌侵染的生理机制 总被引:3,自引:2,他引:1
为深入探讨玉米抗鞘腐病的机制,以高抗品种浚单20、9058及中抗品种郑单958、郑58为材料,测定了接种层出镰孢后苯丙氨酸解氨酶、过氧化物酶、超氧化物歧化酶、多酚氧化酶、过氧化氢酶、木质素含量及抗病相关基因PR-1、PR-2a、LOX、MPI和GAPc的表达量.结果显示:接种后不同抗性材料叶鞘部位的防御酶活性均上升,多数在6h达到峰值,且高抗材料酶活性增幅较大;供试材料的木质素含量均上升,且高抗材料的含量高于中抗材料;PR-1、MPI、GAPc和PR-2a等抗病相关基因的表达呈上升趋势,而LOX的表达量变化趋势不明显.研究表明玉米对鞘腐病的抗性与防御酶活性、木质素含量及其抗病相关基因的表达量呈正相关. 相似文献
136.
137.
Solitary osteochondromas are a relatively infrequent cause of lameness in the horse, most commonly occurring in the caudal distal radial metaphysis, with sparse reports in other locations. Their presence adjacent to a synovial sheath frequently results in intra-thecal tendinous laceration and resultant synovial effusion. Surgical excision in combination with arthroscopic debridement of the tendinous pathology offers the best prognosis for soundness. This report details the successful treatment of a caudodistal tibial osteochondroma using a combination of open surgical excision and tarsal sheath tenoscopy. 相似文献
138.
水稻纹枯病菌核的浮沉特性与超微结构的关系研究 总被引:1,自引:0,他引:1
研究水稻纹枯病菌核的浮沉现象与超微结构的关系及不同生态条件下的菌核结构的差异性,结果表明,菌核具有浮水性,沉水性和转变性等特点,电镜扫描显示菌核的浮沉特性与菌核内外层组织解剖结构有密切关系。外层空细胞腔的厚薄决定菌核的浮沉。浮核外层较厚。沉核外层较薄,用化学药剂处理可使菌核浮或沉,甚至多次反复转变,此外,不同生态条件下形成的菌核其形成结构存在明显差异。 相似文献
139.
Peter Charles Irons 《Australian veterinary journal》2020,98(1-2):48-52
The sheath and prepuce are anatomically simple yet vital parts of the mating apparatus of the bull. They are also susceptible to a range of insults, and pathology of these structures is a common cause of infertility in both Bos taurus and Bos indicus bulls. Significant individual and breed-based variation occurs in the looseness of the skin, the presence and development of preputial muscles, the relative position of the opening to the cranial preputial muscles, the length of the prepuce, and the prominence of the umbilicus. Individual and breed-based susceptibility to different conditions is associated with these anatomical variations. Regardless of the cause, pathology of the prepuce generally results in sepsis, prolapse, stenosis or adhesions or a combination. Clinical examination should be aimed at determining the extent of these processes and the amount of healthy prepuce which would remain following successful treatment. The inclusion of evaluation of sheath conformation in the form of a sheath score is recommended. Current scoring systems do not reflect the diversity of predisposing factors to morbidity and may therefore fail to accurately reflect the risk of morbidity. A more detailed scoring system has been proposed. Further refinement of this system is outlined, consisting of a composite of scores for depth, umbilical skin fold, prepuce eversion, and distance from cranial muscle to opening (the ‘Y’ factor). Validation against actual bull performance is indicated to develop a valid and useful risk-management tool for use in evaluating sheath conformation. 相似文献
140.