氯霉素人工抗原的合成与鉴定   总被引：2，自引：0，他引：2  

方伟  胡鲲  姜有声  黄宣运  杨先乐 《华中农业大学学报》2009,28(3)

采用重氮化法和碳二亚胺(EDC)法合成了氯霉素人工抗原.经紫外光谱法、电泳鉴定表明偶联成功;采用三硝基苯磺酸法测得载体蛋白ε-NH3+残基利用率分别为31.02%、22.27%,偶联物的结合比为18.9、13.6;制备的抗原分别免疫小白鼠获得多克隆抗体,血清效价可达10-6以上.经比较,碳二亚胺法更适于氯霉素人工抗原的制备.  相似文献   

羊奶粉中氯霉素类药物残留液相色谱-串联质谱法的研究     

吴坤  刘李婷  陈姗姗  刘叶  李媛  李超 《中国乳业》2023,(4):92-95

[目的]研究羊奶粉中氯霉素、甲砜霉素、氟甲砜霉素残留的液相色谱-串联质谱测定方法。[方法]样品经乙腈提取,固相萃取柱净化,以甲醇水作为流动相,采用多反应监测负离子模式进行定性及内标法定量分析。[结果]氯霉素、甲砜霉素、氟甲砜霉素的相关系数均大于0.999,氯霉素检出限为0.1μg/kg,甲砜霉素和氟甲砜霉素检出限为0.2μg/kg;氯霉素加标回收率为100.9%～116.2%,甲砜霉素加标回收率为88.4%～99.6%,氟甲砜霉素加标回收率为89.6%～103.2%。[结论]该方法灵敏度高、准确性好,适于测定羊奶粉中氯霉素、甲砜霉素,氟甲砜霉素的残留量。  相似文献   

电子束降解氯霉素调控因子试验     

崔登来  施惠栋  谢宗传 《核农学报》2003,17(5):373-374

电子束降解氯霉素单体水溶液 ,其降解效果与处理条件有关。处理剂量高、单体浓度低和充氮 ,降解效率高 ;低温处理 ;充 0 5 %H2 O2 且pH调高 ,相对降解效率低  相似文献   

抗氯霉素单克隆抗体的制备及其化学发光酶免疫法的建立     

张启模  杨姝  马玲  马玉媛  郭逸  章金刚  吴健敏 《动物医学进展》2011,(8)

以人工合成的氯霉素-牛血清白蛋白(CAP-HS-BSA)免疫Balb/c小鼠,应用杂交瘤技术获得了一株分泌抗氯霉素单克隆抗体(CAP-McAb)的杂交瘤细胞5D7,并对其进行效价、亲和力和特异性测定。结果显示,其分泌的抗体亚类为IgG1,间接ELISA检测细胞培养上清效价为1∶512,诱生的腹水经纯化后效价为1∶2×105,亲和常数为3.6×108L/mol;应用上述单抗建立的检测氯霉素的化学发光酶免疫分析法(ci-CLIA),其检测范围为0.001μg/L~10μg/L,检出限达0.0025μg/L,IC50为39.6μg/L,且与L-苯丙氨酸、L-酪氨酸、青霉素、四环素、庆大霉素等的交叉反应率均小于0.01%,可满足我国对水产品中氯霉素残留检测的要求。  相似文献   

水产品肌肉组织中氯霉素残留量检测方法的对比分析     

李荣  徐进  艾晓辉  邹世平  伍刚  王鹏 《长江大学学报》2006,(11)

初步比较了酶联免疫法(ELISA)中2种氯霉素试剂盒(荷兰EURO-DIAGNOSTICA试剂盒和德国r-Biopharm试剂盒)检测水产品氯霉素残留量的效果,并对测定水产品氯霉素残留量的气相色谱法(GC-ECD)和液相色谱-质谱法(LC-MS/MS)的技术参数进行了比较分析。结果表明,荷兰EURO-DIAGNOSTICA试剂盒和德国r-Biopharm试剂盒的检测范围分别为0.025~2.0μg.L-1和0.05~4.05μg.L-1,其板内相对标准偏差均小于10%,2种试剂盒检测25个鱼或虾样品的假阳性率分别为12%和20%。LC-MS/MS和GC-ECD的最低检测浓度分别为0.1μg.kg-1和0.27μg.kg-1,当氯霉素添加量为2μg.kg-1时其平均回收率分别为95.2%和70.4%;LC-MS/MS样品前处理较简单,GC-ECD较繁琐。  相似文献   

Methicillin‐resistant Staphylococcus aureus keratitis in a dog     

Kazuki Tajima  Akiko Sinjyo  Toshio Ito  Yoshizumi Noda  Hiroshi Goto  Norihiko Ito 《Veterinary ophthalmology》2013,16(3):240-243

The purpose of this study is to report a case of methicillin‐resistant Staphylococcus aureus (MRSA) keratitis in a dog. A 7‐year‐old intact male American cocker spaniel that had undergone removal of a nictitating gland was referred for severe ulcerative keratitis. Slit‐lamp examination showed swelling of the eyelid, mucopurulent discharge, conjunctival injection and chemosis, diffuse corneal edema and opacity, and a deep ulcer in central cornea. Gram staining of discharge from the eye demonstrated Gram‐positive cocci. Despite topical ofloxacin, oxytetracycline and polymyxin B ophthalmic solution and intravenous cefazolin, there was no improvement. Cultures revealed MRSA that was sensitive only to chloramphenicol, vancomycin, lincomycin, and clindamycin. The antibiotic regimen was changed to topical and systemic chloramphenicol. After 9 days of treatment, although inflammation started to be resolved, the dog developed nonregenerative anemia. The antimicrobial regimen was changed again to topical and systemic vancomycin. Inflammation continued to improve over the next week. MRSA should be considered a potential organism in infectious keratitis, especially when general antibiotics are not effective. Although topical and systemic chloramphenicol and/or vancomycin are effective for treating MRSA keratitis, vancomycin should only be used when culture and susceptibility results indicate it is appropriate and no other options are available. To our knowledge, this is the first detailed case report of MRSA keratitis in a dog.  相似文献   

酶联免疫吸附法检测鸡肝肾中氯霉素残留的分析   总被引：3，自引：0，他引：3  

郝俊虎  王仙琴  闫永利  马昕  李萍 《中国家禽》2004,8(Z1):62-64

试验用六种浓度的氯霉素标准品(0.025ng／mL,0.05ng／mL,0.1ng／mL,0.2ng／mL,0.5ng／mL,2ng/mL)做工作标准曲线,设计鸡肝脏、肾脏两个待测样品,同时分别加六种标准品于肝脏和肾脏中测定样品中氯霉素的回收率。试验结果表明:ELESA方法测定鸡组织中氯霉素的检出限为0.02ng／mL,样品中不同浓度的氯霉素标准品回收率均达84.8％以上。说明该方法灵敏、准确。  相似文献   

鸡蛋中氯霉素残留的检测方法及其消除规律   总被引：5，自引：0，他引：5  

袁玉花  陈杖榴  刘力  曾振灵  沈祥广  黄显会 《中国兽医学报》2004,24(5):485-487

选用处于产蛋高峰期的健康海兰蛋鸡20只,单笼饲养,多剂量内服氯霉素(每千克体质量100 mg,连喂12 d),停药后逐只逐日收集鸡蛋,将蛋白、蛋黄分别匀浆,用乙腈、乙酸乙酯、正己烷萃取及净化后,HPLC检测氯霉素浓度.色谱柱为Waters Nova-Pak C18(3.9 mm×150 mm)柱,检测波长为280 nm,流动相为乙腈/水(20/80),流速为1 mL/min,进样量为50 μL.在上述条件下,氯霉素的色谱峰保留时间约为8.0 min,最低检测限为0.002 μg/g,标准曲线的线性范围为0.002～20 μg/g.氯霉素含量为0.5 μg/g时,在蛋白中的批内、批间变异系数分别为3.52%、3.43%;在蛋黄中的批内、批间变异系数分别为3.83%、3.37%.将氯霉素以0.5 μg/g分别添加到空白蛋白和蛋黄中,测得蛋白、蛋黄中氯霉素的回收率分别为94.89%、83.15%.结果表明,本法是一种简便、灵敏、可靠的检测鸡蛋中氯霉素残留的分析方法.停药当天,蛋白、蛋黄中的氯霉素分别为3.61、14.51 μg/g;停药第5天,分别为0.05、7.17 μg/g;停药第8天,蛋白中已检测不到氯霉素,蛋黄中氯霉素为0.80 μg/g;停药第11天,蛋黄中尚有0.04 μg/g的氯霉素,而停药第12天,蛋黄中已检测不到氯霉素.采用MCPKP药动学参数计算程序处理药时数据,算出蛋白、蛋黄中的消除半衰期分别为0.75、1.64 d.结果表明,氯霉素多分布于蛋黄,在蛋黄中的残留水平高,且在蛋黄中的消除较蛋白缓慢.  相似文献   

氟苯尼考对鸡多杀性巴氏杆菌感染的疗效试验   总被引：1，自引：0，他引：1  

缪小群  宋冶萍  黄显会  曾振灵 《中国兽药杂志》2006,40(8):52-55

以试管肉汤两倍稀释法测定氟苯尼考和氯霉素对鸡多杀性巴氏杆菌的最小抑菌浓度，然后对实验性鸡多杀性巴杆菌感染进行内服或肌注给药3d（每天2次）的治疗试验。结果表明，氟苯尼考和氯霉素对鸡多杀性巴氏杆菌的最小抑菌浓度分别是0．5和1．0μg／mL。以20、40、80mg／kgBW氟苯尼考和40mg／kgBW氯霉素的剂量给鸡内服给药后，对鸡多杀性巴氏杆菌感染的治愈率分别为93．3％、100％、100％及70％；相同剂量肌注给药的治愈率分别为86．7％、100％、100％和83．3％，而感染对照组的死亡率为90．0％。  相似文献   

Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China     

Jingfan Xiao  Qiyao Wang  Qin Liu  Xin Wang  Huan Liu  Yuanxing Zhang 《Aquaculture Research》2008,40(1):13-17

The causative agent was isolated from diseased turbots (Scophthalmus maximus) stricken by a high‐mortality outbreak of bacterial septicaemia occurring in a mariculture farm in Yantai, a northern coastal city of China. Seven pure isolates, namely EH‐15, EH‐103, EH‐107, EH‐202, EH‐203, EH‐305 and EH‐306, belonged to Edwardsiella tarda. The phenotypic features of the cultures were analysed extensively. Three of the isolates showed high 16S rDNA sequence similarities with E. tarda sequence (GenBank accession no. EF467289). However, unlike the E. tarda ATCC 15947, all the isolates, except EH‐15, contained a novel large plasmid sized about 23.7 kb. Furthermore, pathogenicity of the isolates was addressed by experimental challenges with fish models. The isolates exhibited strong virulence to swordtail fish with LD₅₀ ranging between 3.8 × 10³ and 3.8 × 10⁵ CFU g^?1, and EH‐202 displaying the lowest LD₅₀ value among them. Antibiotic susceptibilities of E. tarda isolates were assayed. Compared with E. tarda ATCC 15947, the isolates displayed strong resistance to chloramphenicol, and the probable dominant chloramphenicol resistance determinant was cat III. Depicting the main biological properties of turbot‐borne E. tarda strains in China, the study provided useful information for further unveiling their pathogenic mechanisms.  相似文献