牛胎儿成纤维细胞分离与体外培养   总被引：2，自引：1，他引：2  

徐小明  窦忠英  华进联  葛秀国 《中国畜牧杂志》2004,40(10):18-21

本研究以 4 5d牛胎儿为材料 ,使用 0 .2 5 %胰酶 + 0 .0 4 %EDTA消化液 ,分离得到牛胎儿成纤维细胞 ,体外培养传代至第 2 8代。本实验描绘出牛胎儿成纤维细胞生长曲线 ,并发现α MEM是一种适合牛胎儿成纤维细胞生长的培养基。分离得到的牛胎儿成纤维细胞传至第 1 8代时核型仍然正常 ,冷冻、解冻后细胞可继续传代  相似文献   

Effect of sweet potato vines on performance parameters and some carcass characteristics of rabbits          下载免费PDF全文

A. C. K. Klinger  L. P. da Silva  G. S. P. de Toledo  B. Galarreta  F. Goulart 《Journal of animal physiology and animal nutrition》2018,102(1):152-156

Conventional feedstuffs in Brazil are expensive, which has led to the search for less conventional cheaper and locally available feedstuffs. Thus, this study was carried out to determine the dietary effect of dry sweet potato vines (SPV) on the performance and some carcass characteristics of rabbits. A total of twenty‐seven weaned White New Zealand rabbits (14 male and 13 female) 35 days old with an average initial weight of 755 g were allocated into three treatments. Nine rabbits were assigned to each treatment in a completely randomized design. Three diets were formulated to similar protein and energy levels: 0SPV – diet without inclusion of dry sweet potato vines; 10SPV – diet with 10% alfalfa hay replaced by dry sweet potato vines; and 15SPV – diet with 15% of alfalfa hay replaced by dry sweet potato vines. The experiment lasted for 49 days during which data for feed intake and body weight were recorded. The daily feed intake ranged from 73.17 to 78.02 g; daily weight gain from 22.32 to 23.17 g; feed conversion ratio (FCR) from 3.16 to 3.49 and final live weight ranged from 1839.44 to 1880.55 g. None of the evaluated performance parameters as well as carcass weight, heart weight and percentages of fat and protein in meat were significantly affected by any of the tested dietary treatments. However, the liver weight was statistically lower in animals fed the diets containing 10% of SPV, mainly due to lower glucose content. Glycogen within the tissue, did not differ significantly among treatments. It was therefore concluded that up to 15% of SPV can successfully be included in the diet of rabbits as a cheaper replacement for alfalfa hay without adversely affecting performance.  相似文献   

规模化养殖场群体动物中草药服用方法的改进及其应用     

吕玉卓  王忙生 《现代畜牧兽医》2014,(1):38-40

利用中草药防治动物疾病已经成为养殖业研究的重要课题，但对动物服用中草药的难度较大，该文改进了传统的人工胃管灌服方法的缺陷，采用将中草药与精料共煮后饲喂，方法简便、价格低廉、节省人力，对规模化养殖场群体动物的中草药应用具有良好的推广价值。  相似文献   

花生副产物在畜牧生产中的综合利用     

郭爱红  ;张号杰 《饲料与畜牧》2014,(11):13-15

花生在生产过程中产生大量副产物,其营养价值较高,是重要的饲料加工原料。文章主要针对花生生产过程中副产物营养价值及在畜牧生产中的利用进行综述。  相似文献   

心房钠尿肽基因敲除小鼠的鉴定     

杨扬  李斌  叶杰  亓翠玲  何晓东  李江超  章倩倩  黄韧  张钰  王丽京 《动物医学进展》2015,(1):71-74

研究心房钠尿肽基因敲除小鼠(ANP-/-小鼠)的生物学及病理学特性。ANP-/-小鼠与C57BL/6小鼠饲养于SPF级环境中,观察小鼠的繁育状况及其生物学特性,选取合适时间处死小鼠,通过HE染色观察脏器形态及组织结构。ANP-/-小鼠的繁殖周期比C57BL/6长,产仔率较低;ANP-/-小鼠肝脏、肺脏、脾脏较C57BL/6小鼠的有明显炎症反应,但是C57BL/6小鼠未见明显的病理改变。提示ANP基因的缺失可能与炎症发生有关。说明ANP-/-小鼠繁殖较慢、产仔率低,且正常繁殖的普通小鼠不会引起器官的病理学改变。但作为一种在心血管、代谢疾病上的动物模型,并与炎症甚至肿瘤有可能的未知联系的转基因动物,其很有研究和探索价值。  相似文献   

How to Assess Stress in Reptiles     

《Journal of Exotic Pet Medicine》2014,23(3):240-243

Stress, as an adaptive response of any animal to a stimulus that presents a threat to homeostasis, can occur in reptiles. Yet, many veterinarians fail to recognize the signs of stress in reptiles. In this article, evaluation of reptile stress has been discussed with a focus on the analysis of behavioral changes, neuroendocrine correlation, and biochemical and physiologic effects in reptiles. If stress can be assessed and treated properly in captive reptiles, their health and well-being can be optimized.  相似文献   

动物生理学实验教学单独设课之重要性     

陈晓光  吕琼霞  刘玉梅  邓雯 《畜牧与饲料科学》2014,(2):29-31

在高校的动物生理学教学中,实验教学是其中的一个重要组成部分。作为课堂教学的重要辅助和补充,动物生理实验教学效果对课堂教学具有极大的驱动作用。为突出动物生理实验教学的重要性,对动物生理学实验施行单独设课,这是考查理论教学效果、促进实验课教学质量的一种重要措施。  相似文献   

“虚拟仿真”背景下畜牧兽医研究生实践教学改革探索研究     

余燕  聂国兴  徐之勇  王莉  王秋霞  胡东方  马金友 《现代农业科技》2020,(7)

研究生教育位于国民教育体系的顶端，是国家高等教育发展水平的重要标志。为培养 既具有责任担当精神，又具有宽厚的专业知识和创新能力，还具有较高的英语水平和较强的计算机网络应用能力的高层次畜牧兽医人才，本研究对我校畜牧兽医专业研究生的培养现状进行了分析总结，提出了基于“虚拟仿真”背景下人才培养的新模式，总结了虚拟仿真研究生科研平台建设的优势和使用效果，为其它专业研究生的培养提出参考。  相似文献   

Phosphorus availability and farm structural factors: examining scarcity and oversupply in north‐east Germany          下载免费PDF全文

T. F. H. Theobald  K. Daedlow  J. Kern 《Soil Use and Management》2015,31(3):350-357

Assessing factors influencing phosphorus (P) availability in soils is important in preventing its overexploitation and excessive application in agricultural systems. Despite high historical P applications in the federal state of Brandenburg (Germany), county data on soil test P (STP) reveal considerable disparities in soil available P. In addition, negative soil balances as a result of small mineral P and manure inputs have been observed, raising questions about the factors leading to this situation. Our work focused on identifying possible causes operating at the farm management level by conducting a letter survey in two administrative districts of Brandenburg, the counties Barnim and Uckermark, linking farm management factors (ownership type, farm size, land tenure, animal husbandry with or without grassland and its intensity, presence of a biogas plant and organic production) to farmers’ self‐indicated levels of STP. Small‐ to medium‐sized individual farms tended to have (very) high STP, while large partnership farms and companies/cooperatives were sensitive to factors resulting in low STP. Farms with low shares of land ownership, the presence of grassland, extensive cattle farming and stockless organic farming had lower STP. On the other hand, biogas plants, partly in combination with intensive livestock (cattle) farming, were associated with larger STP. It was concluded that more care should be devoted to the design of agricultural policies and that further (inter‐ and transdisciplinary) research on this topic is needed.  相似文献   

Rabbit Behavior     

《Veterinary Clinics of North America: Exotic Animal Practice》2021,24(1):53-62

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