Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System     

HAO Yu-meng  ZHAO Feng  ZHANG Na  CHEN Dan  LI Qing-zhang 《中国畜牧兽医》2016,43(8):2095-2103

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.  相似文献   

黑龙江农垦齐齐哈尔管理局奶牛养殖小区饲养经营模式及效益的探析     

宋利国  齐楷  张莹 《黑龙江八一农垦大学学报》2014,(3):30-34

对农垦齐齐哈尔管理局奶牛养殖小区的饲养经营模式及效益进行了调查,分析了奶牛小区建设给奶牛群带来的主要变化。通过分析不同模式奶牛小区生产经营状况,确定适合该地区奶牛业发展的奶牛小区经营模式。  相似文献   

红花粕和水飞蓟粕对生长獭兔的营养价值评定     

吴峰洋  李冲  陈赛娟  刘亚娟  谷子林 《动物营养学报》2017,29(2)

本试验旨在通过饲养试验和消化试验来评定红花粕和水飞蓟粕在生长獭兔上的营养价值。选取18只60日龄左右、平均体重(1.73±0.21)kg、健康状况良好的白色獭兔,随机分为3个组,每组6个重复,每个重复1只兔。各组分别饲喂基础饲粮、红花粕饲粮(85%基础饲粮+15%红花粕)和水飞蓟粕饲粮(85%基础饲粮+15%水飞蓟粕)。预试期和正试期各5 d。采用化学分析法测定红花粕和水飞蓟粕的总能(GE)及各营养物质含量,采用全收粪法测定生长獭兔对各营养物质的表观消化率。结果表明:红花粕和水飞蓟粕中的GE、干物质(DM)、粗蛋白质(CP)、粗纤维(CF)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、酸性洗涤木质素(ADL)、粗脂肪(EE)、粗灰分(Ash)、钙(Ca)、磷(P)以及无氮浸出物(NFE)的含量分别为:18.81 MJ/kg、93.76%、23.94%、14.95%、19.92%、11.99%、2.19%、1.64%、4.93%、0.37%、0.57%、49.32%与17.12 MJ/kg、91.94%、23.62%、16.21%、38.57%、22.73%、4.04%、2.07%、5.31%、0.29%、0.68%、42.09%。生长獭兔对红花粕和水飞蓟粕中GE、DM、CP、CF、NDF、ADF、EE、Ash、Ca、P和NFE的表观消化率分别为62.60%、61.72%、62.39%、15.68%、26.30%、14.75%、80.69%、38.35%、59.35%、31.98%、79.61%与63.13%、61.94%、68.01%、15.74%、27.64%、14.98%、79.90%、38.20%、60.44%、32.99%、79.81%。由此可见,生长獭兔对红花粕和水飞蓟粕中的不同营养成分的表观消化率存在一定差异,结合2种原料各营养物质含量总体分析,2种原料均可作为獭兔的蛋白质饲料资源应用,并且对生长獭兔的营养价值相近。  相似文献   

激素对奶牛乳腺上皮细胞乳脂肪合成的调控作用     

曹越  李大彪 《中国畜牧兽医》2021,48(8):2778-2786

乳脂肪是高质量的天然脂肪，其可为人类提供营养和能量，在各种膳食脂肪和油类中，是最容易被消化吸收的。乳脂肪是在乳腺中由从头合成或外源摄取的脂肪酸与甘油酯化形成的一种脂类物质，其含量的高低关系着牛奶品质的优劣和乳制品的加工特性。在奶牛的泌乳周期中，乳腺泌乳功能受多种因素影响，其中内分泌腺分泌的多种激素对奶牛乳腺上皮细胞（BMECs）乳脂的合成具有积极的调控作用。综上所述，作者介绍了氢化可的松、催乳素、胰岛素和生长激素4种泌乳相关激素对BMECs乳脂肪合成的调控机理，即从乳脂合成适宜的激素添加量、激素对乳脂球形态的影响方面初步阐释其调控作用，并从乳脂合成的关键酶及转录因子、激素对乳脂合成相关基因表达量方面深入阐释其作用机理，旨在为研究泌乳相关激素对奶牛乳腺内乳脂肪合成的调控机理提供参考。  相似文献   

不同药物防治羔羊奶结症临床效果分析     

王选慧  ;王治仓  ;任作宝  ;黄静  ;王喜军  ;王丽娟 《中兽医医药杂志》2014,(5):44-45

为寻找羔羊奶结症的有效防治药物，将筛选出的5组药物用于羔羊奶结症的防治试验？结果表明，第l组药物“消维康”口服液对于减少羔羊奶结的发生有明显作用，第2组药物四仙莪棱香附散治疗羔羊奶结效果最佳．  相似文献   

晚稻机收留高茬紫云英迟播技术的应用效果研究   总被引：2，自引：0，他引：2  

鲁艳红  廖育林  周兴  谢坚  周升明  杨曾平  聂军 《湖南农业科学》2014,(20)

通过田间试验,研究了双季稻田晚稻机械收获留高茬后紫云英迟播的应用效果。结果表明,与传统紫云英播种技术相比,晚稻机械收获留高茬紫云英迟播显著缩短了紫云英的生育期,但其盛花期能保持与传统播种技术一致,对下茬早稻作物的种植没有影响;两种播种方法对盛花期鲜草产量、植株养分含量和养分积累量均没有显著影响,说明迟播播种能达到与传统播种一样的肥田目的;迟播对紫云英的种子产量及产量构成因素均没有明显影响,与传统播种技术相比可节约劳动力成本约45元/hm2,且可避免传统播种方法对晚稻植株的伤害。在现代双季稻种植条件下,晚稻机械收获留高茬紫云英迟播技术具有较大的优势和发展前景。  相似文献   

Associations between postpartum diseases and milk yield and changes in body condition between drying off and parturition of dairy cows in Argentina     

P Melendez  F Bargo  G Tuñón  J Grigera 《New Zealand veterinary journal》2020,68(5):297-303

ABSTRACT

Aims: To assess the change in body condition score (BCS) during the early and late dry periods and its association with postpartum diseases and milk yield in grazing dairy cows from central Argentina.

Methods: BCS assessments during the dry period, and cow health and milk production records up to 90 days in milk (DIM), were collated for cows from 28 farms at monthly visits between 2007 and 2008. Cows were categorised into four groups; those in Group 1 (n=7,067) maintained or gained BCS during the early and late dry periods; Group 2 (n=2,615) maintained or gained BCS during the early dry period and lost BCS during the late dry period; Group 3 (n=1,989) lost BCS during the early dry period and maintained or gained BCS during the late dry period; and Group 4 (n=5,144) lost BCS during the early and late dry periods.

Results: Cows in Group 1 had reduced odds of having retained fetal membranes (RFM), metritis, and clinical mastitis up to 90 DIM than cows in Group 2 (p<0.001), but the odds of disease were similar to cows in Group 3. The odds of having RFM or clinical mastitis tended to be lower in cows in Group 1 than cows in Group 4 (p=0.08). The odds of cows being culled or dying during the first 90 DIM were lower for cows in Group 1 than for those in Groups 2, 3, and 4 (p≤0.05). Mean accumulated milk yield up to 90 DIM was higher in cows in Group 1 than Group 2 and Group 4 (p<0.001), but was similar to that of cows in Group 3 (p=0.28).

Conclusions and clinical relevance: Cows that lost BCS during the late dry period had increased odds of being diagnosed with several postpartum diseases and had decreased milk yield compared to cows that maintained or gained BCS during the entire dry period. Loss of BCS during any stage of the dry period was also associated with increased incidence of culling or death during the first 90 DIM. These results should raise awareness among dairy cattle producers of the importance of properly managing cow body condition during the dry period, especially during the late dry period.  相似文献   

液态奶生产新设备和工艺     

郭瑞雪  郭俭 《中国乳业》2021,(12):137-140

液态奶包括巴氏杀菌奶、UHT超高温灭菌奶等。目前国内采用的液态奶杀菌工艺是热处理法。但通过热处理法的液态奶仍可能残留休眠芽孢,给液态奶产品带来潜在风险。本文通过研究目前液态奶杀菌工艺的种类和存在问题,申报了发明专利“一种能延长液态奶保质期的生产方法”和“一种能使液体隔离空气的无菌罐体”,并利用氢医学制作含氢乳制品,实现了提高液态奶品质,延长保质期,获得氢分子医学效应。  相似文献   

中国奶粉进口贸易发展的新态势、问题与建议     

郑国富 《中国乳业》2021,(9):52-59

2001—2020年中国奶粉进口贸易发展呈现规模持续增扩与地位提升,产品结构趋于优化,进口来源有效拓展,进口均价持续攀升等特征。但也存在着进口增势迅猛,冲击国内产业;来源市场集中,均价不断高涨;假洋奶粉泛滥,扰乱市场秩序;走私花样繁多,海关监管困难等问题。提出新时期应积极扶持国产奶粉生产企业转型升级,提升核心竞争力;拓展来源渠道,分散风险集聚;整顿行业乱象,规范市场秩序;从严海关监管,打击非法走私,实现国内奶粉行业可持续健康发展。  相似文献   

产前和产后香味剂印迹训练对仔猪断奶后采食偏好的影响及其机理     

张鑫  李方方  朱宇旌  郑丽莉  张勇 《动物营养学报》2015,(6)

仔猪断奶后受到心理、营养、环境等多方面的刺激,导致应激反应,影响仔猪的采食量。固体饲粮代替母乳是最大的应激源,通过在母猪饲粮中添加香味剂对仔猪产前和产后进行香味剂印迹训练,使仔猪逐渐喜欢这种气味,降低其对添加相同香味剂饲粮的应激反应,并对有相同气味的饲粮有采食偏好,从而提高仔猪的采食量。本文对产前和产后香味剂印迹训练对仔猪断奶后采食偏好的影响及其机理进行综述,为养猪业相关试验研究提供理论依据。  相似文献