鸡新城疫病毒B_(95)株的RT-PCR检测方法研究     

申之义  苗儒  王俊平  焦静波  信洪一  李平安 《中国动物检疫》2003,(4)

本试验设计了一对B95 株的特异引物NDV -P1/P2,探讨了对其进行RT-PCR检测的可行性。结果证明 ,用该引物可以扩增出一条310bp的特异核酸带 ,敏感性为0.0043ng/ul。  相似文献   

丛生竹防霉处理研究     

许斌  张齐生 《竹子研究汇刊》2006,25(4):28-30,31

通过选用多种防霉剂,采用不同的处理方法来对丛生竹进行防霉处理,分别进行室内存放1 a后检验和恒温恒湿箱快速检验来判定防霉效果,对几种处理方法的优缺点进行比较,从中筛选出针对不同使用场合处理的优良防霉剂。  相似文献   

不同包被液对ELISA检测五号病病毒抗体的比较试验     

杨傲冰  杜伟贤 《广东畜牧兽医科技》1995,20(2):7-9

０．０５ＭｐＨ９．６碳酸盐缓冲液和０．０５ＭＰＨ１３ＮａＯＨ溶液作为包被液，分别用于间接ＥＬＩＳＡ检测乙型五号病病毒抗体。结果表明，用０．０５ＭＰＨ１３ＮａＯＨ溶泡包被可完全灭活五号病病毒抗原而用０．０５ＭＰＨ９．６碳酸盐缓冲液却有８７％的五号病病毒抗原未被杀灭；用于检测时，以０．０５ＭＰＨ１３ＮａＯＨ溶液为包被液在敏感性及检测结果的梯度方面都优于用０．０５ＭＰＨ９．６碳酸盐缓冲液。  相似文献   

妊娠黄牛的血液动力流变学观察     

巩忠福  黄虎祖等 《青海畜牧兽医杂志》1995,25(6):25-26

首次应用XXG－A型心血管功能测试仪以无创伤检测技术对16例妊娠黄牛22项血液动力流变学指标做了检测，并首次对怀孕黄牛的血液动力流变学特征做了报道。  相似文献   

Development of a semi-selective medium and an immunofluorescence colony-staining procedure for the detection of Clavibacter michiganensis subsp. sepedonicus in cattle manure slurry     

N. J. M. Roozen  J. W. L. Van Vuurde 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(5):321-334

Various compounds and basal media were tested for their suitability to create a semi-selective medium for isolation ofClavibacter michiganensis subsp.sepedonicus (Cms) from cattle manure slurry containing c. 10⁸ colony forming units (cfu) per ml.Plating efficiency of Cms in yeast glucose mineral medium (YGM) was 104% compared with yeast peptone glucose medium. Nalidixic acid, polymyxin B sulphate and the experimental disinfectant S-0208 inhibited colony growth of cattle slurry bacteria as compared with Cms in YGM. The optimal concentration of these inhibitors in combination was determined by modified agar diffusion tests and by pour plating in 24-well tissue culture plates. The semi-selective medium YGMI consisted of YGM supplemented with nalidixic acid (2 mg/l), polymyxin B sulphate (30 mg/l) and S-0208 (125 mg/l). Plating efficiency varied for Cms between 50.9 and 69.6%, for cattle slurry bacteria between 1.8 and 2.5% and for saprophytes from potato heel end extracts between 11.5 and 27.4%.Differentiation of Cms colonies from other colonies was based on their small and bluish colony morphology in pour plates and on immunofluorescence colony-staining (IFC). IFC of a pure culture of micro colonies of Cms in YGM was possible after one day incubation (colonies c. 5 cells). Green background fluorescence in the agar gels was prevented by addition of Tween 20 (0.1%) to the washing buffer and the use of 1% agar gels. IFC of macro colonies of Cms in YGMI, visible with 4x objective magnification, was possible after 4 days. The detection level of the target organism in artificially inoculated cattle slurry in YGMI based on colony morphology varied between 1.4×10³ and 2.3×10⁴ cfu per ml of cattle slurry. Miniaturized plating combined with IFC, using wells in tissue culture plates (=16 mm), proved suitable for detection, but was c. 30 times les sensitive. The recovery of Cms was negatively correlated with the number of saprophytic colonies in the agar plates (R ²=0.74).  相似文献   

非线性方程组拟牛顿法中线性搜索的一种改进     

李董辉  张忠志 《湖南农业大学学报(自然科学版)》1996,23(4)

改进了Ｇｒｉｅｗａｎｋ（１９８６）提出了关于求解非线性方程组的一种线性搜索方式。在理论上保证了线性搜索的实现,使得算法是适定的,而且,在改进的线性搜索条件下,Ｂｒｏｙｄｅｎ算法仍具有全局收敛性和局部超线性收敛性。  相似文献   

苹果退绿叶斑病毒组织印迹的免疫法检测与定位     

王乔春 《果树学报》1996,(Z1)

用组织培养技术将富士苹果茎尖外植体建立在ＭＳ培养基中,形成试管苗。取４—６周龄的茎与愈伤组织,徒手切取约１ｍｍ厚的横切面,印迹在硝化纤维膜上。印迹组织经封闭后,与ＣＬＳＶ碱性磷酸酶标抗体反应。对反应结果进行显色。感染ＣＬＳＶ的印迹组织呈紫色反应,正常组织无显色反应。结果表明,该方法十分容易检测印迹组织中的ＣＬＳＶ。带毒愈伤组织较茎的显色反应敏感,显色反应时间仅为茎的一半（１５ｍｉｎ）。ＣＬＳＶ在愈伤组织中有两种分布情况：一是所有组织均有显色反应;二是呈不均匀分布。ＣＬＳＶ在茎组织中有三种分布情况：第一,除髓部外,表皮、皮层及维管系统中均有分布;第二,不均匀分布,ＣＬＳＶ集中分布于表度组织中,在皮层及韧皮部仅有少量分布;第三,“嵌合”分布,即同一种组织中部分组织带毒,部分为正常组织。  相似文献   

Serum 5'nucleotidase activity in rats: a method for automated analysis and criteria for interpretation     

Carakostas MC  Power RJ  Banerjee AK 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(4):109-113

A manual kit for determining serum 5'nucleotidase (5'NT, EC 3.1.3.5) activity was adapted for use with rat samples on a large discrete clinical chemistry analyzer. The precision of the method was good (within-run C.V. = 2.14%; between-run C.V. = 5.5%). A comparison of the new automated method with a manual and semi-automated method gave regression statistics of y = 1.18X -3.66 (Sy. x = 4.54), and y = 0.733X + 1.97 (Sy. x = 1.69), respectively. Temperature conversion factors provided by the kit manufacturer for human samples were determined to be inaccurate for converting results from rat samples. Analysis of components contributing to normal variation in rat serum 5'NT activity showed age and sex to be major factors. Increased serum 5'NT activity was observed in female rats when compared to male rats beginning at about 5 to 6 weeks of age. An analysis of variance of serum 5'NT, alkaline phosphatase, and GGT activities observed over a 9-week period in normal rats suggests several advantages for 5'NT as a predictor of biliary lesions in rats.  相似文献   

柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布   总被引：3，自引：0，他引：3  

彭军  周常勇  唐科志  谭万忠 《果树学报》2005,22(6):744-747

柑橘裂皮病是由柑橘裂皮病类病毒(Citrusexocortisviroid,CEVd)引起的一种重要的柑橘病害。分别采用快速微量核酸提取法和SDS-KAc抽提法在表现症状的Etrog香橼中提取核酸,采用一步RT-PCR法对CEVd进行检测,结果显示SDS-KAc法可以消除带毒样品中非特异性条带。从嫁接接毒的铜水72-1锦橙植株的接穗部取嫩叶、老叶、皮,从砧木部茎杆上取老皮以及根皮分别提取总核酸进行RT-PCR检测,分析CEVd在甜橙体内的分布情况。初步检测结果表明在接穗的嫩叶、老叶、皮,砧木部茎杆的老皮和根皮上都可以检测到CEVd,以接穗部叶和皮检出稳定性高。  相似文献   

应用PCR技术检测空肠弯曲菌及结肠弯曲菌的研究     

王振国  刘金华  罗雁菲  蔡阳  肖成蕊  刘和平  徐宝梁  王岸英 《中国家禽》2005,9(Z1):133-135

本研究根据空肠弯曲菌及结肠弯曲菌的16s rRNA基因相同保守序列来设计引物,建立了一种PCR检测方法,该方法具有较强的特异性及灵敏性,其对空肠弯曲菌及结肠弯曲菌的最低检测限度为5 CFU/mL,它无需增菌培养过程可以直接对样品进行检验,在对鸡肉样品空肠弯曲菌及结肠弯曲菌的检测中取得了较好的效果.  相似文献