Cell direct reprogramming：a new technique for treating diabetes     

REN Li-wei  YANG Xiao-fei  LI Fu-Rong 《园艺学报》2014,30(12):2284-2288

Diabetes is characterized by an absolute or relative deficiency in β-cell mass, which cannot be reversed with existing therapeutic strategies. The restoration of the endogenous islet β-cells can stabilize the level of blood glucose. The islet β-cells can be obtained from the directional differentiation of stem cells, but the process is complex and has the risk of teratomas generation. Cell direct reprogramming, one terminal differentiated cell can transdifferentiate into another kind of terminal differentiated cell, which is other than directional differentiation from stem cells. Direct reprogramming gives rise to the generation of islet β-cells from one terminal differentiated cell, may be preferable for diabetes therapy because of its unique advantage.  相似文献   

粮食污染物残留免疫分析技术研究与示范     

张少波  张文生  李文辉  陈嘉东  许艇  郑妙  张小松  刘志平  肖治理  刘剑锋  万红艳  黄金萍  田巍  葛俊  赵娜 《粮食储藏》2017,(4):5-14

随着人们对粮食安全问题的重视,政府部门不断加强对粮食安全的监测工作。针对粮食生产、加工、储运过程中的化学污染物安全性问题,研究粮食污染物快速监测技术,研究污染物免疫分析方法通过利用抗原、抗体的特异性反应来检测农药含量,设计并筛选吡虫啉、多溴联苯醚、功夫菊酯、氰戊菊酯4种半抗原,制备相关高特异性的抗体,分析吡虫啉、多溴联苯醚、功夫菊酯与气相色谱法(GC)相比,稻谷、大米、玉米样品通过相关性分析均高度相关,操作简便、快速。  相似文献   

甜菜DAMD-PCR体系的建立及优化   总被引：2，自引：2，他引：0  

兴旺  童乐怡  孙燕红  马龙彪  吴则东 《中国农学通报》2017,33(23):6-9

为了建立甜菜DAMD扩增体系,以期利用DAMD引物应用于甜菜品种指纹图谱的构建及分子标记辅助育种。本实验利用单因素变量的方法对甜菜DAMD体系进行优化。同时选用12个甜菜品种,利用优化的体系对25条DAMD引物进行扩增。获得甜菜的最适DAMD体系:总体积为20μL,包含模板DNA 10~80 ng、0.75 U的DNA聚合酶、0.2μL的d NTPs(2.5 mmol/L each)以及2.0μL的引物(10μmol/L)。同时25条引物均扩增出了清晰条带,除了个别引物多态性较差外,其余引物多态性都非常的丰富,其中引物62H(-)就可以把实验中用到的12个甜菜品种全部区分开。由此可见,DAMD引物的扩增效率很高,并且扩增结果稳定,条带清晰,非常适合甜菜品种指纹图谱的构建及遗传多样性分析。  相似文献   

氨基修饰二氧化硅对Cr^3+和直接桃红B12的吸附     

李琼  胡育  宋连香  刘素君  王应红 《中国沼气》2020,(1):49-52

该试验以硅烷偶联剂KH-550为改性剂,乙醇为溶剂合成氨基二氧化硅。利用红外光谱、扫描电镜、原子吸收光谱、可见分光光度法对改性前后SiO 2进行表征,研究其对铬离子及染料直接桃红B12的吸附情况。结果表明:25℃恒温振荡20 min,改性SiO 2吸附重金属离子Cr^3+效果明显,当其用量为0.04 g时,吸附率达99.01%,而相同条件下未改性SiO 2对Cr^3+吸附率只有25.13%;将其用于染料直接桃红B12的吸附,改性后SiO 2吸附率达91.97%,相同条件下未改性SiO 2的吸附率仅为34.23%。  相似文献   

机械化直播油菜的优点及高产栽培技术     

顾宗福  孙华  邵慧霞  仲嘉  穆晓伟 《安徽农业科学》2016,44(14):27-28

针对近年来油菜生产机械化程度低、用工多、成本高、效益低、面积逐年萎缩的情况,通过多年试验与总结,探索与完善了机械化直播油菜的高产栽培技术,实现了省工节本、提高机械化水平的目的,也为大面积油菜生产提供技术参考。  相似文献   

调环酸钙25%悬浮剂对直播稻抗倒伏和产量性状的影响     

郑先福  ;郑昊  ;骞天佑  ;刘彦涛  ;万翠  ;禹震洋 《农药科学与管理》2014,(9):59-62

为了研究新型植物生长调节剂调环酸钙在直播稻上的应用效果,本研究设计了调环酸钙25%悬浮剂在直播稻武运粳8号上的田间试验,用来了解其对直播稻的抗倒伏性状和产量性状的影响。试验结果表明:在直播稻武运粳8号的分蘖期使用调环酸钙25%悬浮剂12~15g/667m2均能够显著降低水稻的株高,缩短茎基部节间长度,提高水稻的抗倒伏能力,同时也能显著提高水稻产量。  相似文献   

近自然生态条件下千年桐竞争关系的研究     

胡明娇  刘玉玲  刘钢  彭辉武  李祥  潜伟平  刘江华  刘小艳 《江西林业科技》2014,(3):9-11,62

以近自然生态条件下千年桐直播造林林分为研究对象，研究其种内种间竞争关系。结果表明：直播造林林分中千年桐胸径＜8 cm时，千年桐种内竞争较弱，胸径在8～13 cm时，其竞争指数增长特别快，种内竞争非常大；当胸径＞13 cm时，种内竞争指数逐渐减少；其种间竞争中与杉木的竞争最为强烈。千年桐种内竞争与距离的关系表现为当其距离＜1.0 m时，其竞争指数最高，种内竞争最为激烈；距离在2.0～3.5 m时，种内竞争指数较为稳定；当其距离超过3.5 m后，千年桐种内竞争指数逐渐变小，竞争强度逐渐减弱。  相似文献   

施氮量对油后直播棉花产量、品质及生物量的影响   总被引：1，自引：0，他引：1  

李飞  郭利双  李彩红  郭莉莉  刘冰蕾  张志刚  梅正鼎 《核农学报》2020,34(9):2088-2094

为研究施氮量对油后直播棉花生物量累积特征及其与产量、品质的关系,于2016-2017年在湖南常德设置了施氮量分别为0、90、180、270、360 kg·hm^-2氮肥水平试验。结果表明,在施氮量为0~360 kg·hm^-2范围内,施氮量越大棉株营养器官生物量累积越多,而棉株生殖器官生物量则在施氮量为270 kg·hm^-2时累积最多;皮棉产量、成铃数和单铃重均在施氮量为270 kg·hm^-2时达到最大;0~270 kg·hm^-2范围内,衣分随施氮量增加而增加。在施氮量为270 kg·hm^-2条件下,纤维长度和断裂比强度最高;适宜施氮量有利于纤维长度、断裂比强度的提高和马克隆值的优化,对伸长率和整齐度基本无影响。综上所述,适宜施氮量可提高棉花的产量、品质,洞庭湖棉区适宜的施氮量为180~250 kg·hm^-2。本研究结果为环洞庭湖棉区的氮肥合理运筹提供了技术参考。  相似文献   

Assessment of plasma anti-elastin antibodies for use as a diagnostic aid for chronic progressive lymphoedema in Belgian Draught Horses     

《Veterinary immunology and immunopathology》2015,163(1-2):16-22

Diagnosis of chronic progressive lymphoedema (CPL) in draught horses, including the Belgian Draught Horse, is mainly based on clinical evaluation of typical lower limb lesions. A deficient perilymphatic elastic support, caused by a pathological elastin degradation in skin and subcutis, has been suggested as a contributing factor for CPL. Elastin degradation products induce the generation of anti-elastin Ab (AEAb), detectable in horse serum by ELISA. For a clinically healthy group of draught horses, a significantly lower average AEAb-level than 3 clinically affected groups (mild, moderate and severe symptoms) was demonstrated previously. To improve CPL-diagnosis, we evaluated the AEAb-ELISA as an in vitro diagnostic aid in individual horses. Test reproducibility was assessed, performing assays independently in 2 laboratories on a total of 345 horses. Possible factors associated with AEAb-levels (age, gender, pregnancy, test lab and date of blood collection) were analyzed using a mixed statistical model. Results were reproducible in both laboratories. AEAb-levels in moderately and severely affected horses were significantly higher than in healthy horses. Nevertheless, this was only demonstrated in barren mares, and, there was a very large overlap between the clinical groups. Consequently, even when a high AEAb cut-off was handled to obtain a reasonable specificity of 90%, a very low sensitivity (21%) of AEAb for CPL-diagnosis was obtained. Results on the present sample demonstrate that the described ELISA procedure is of no use as a diagnostic test for CPL in individual horses.  相似文献   

Seroprevalence,associated risk factors analysis and first molecular characterization of chlamydia abortus among Egyptian sheep     

《Comparative immunology, microbiology and infectious diseases》2021

Chlamydia abortus is one of the most common abortive agents worldwide in sheep. Few studies have been reported C. abortus infection among sheep in Egypt but the available data is scarce. The objective of the present study was to determine the seroprevalence of C. abortus among sheep, the associated risk factors and its molecular characterization. The present study was conducted on 675 sheep in six Governorates at Northern Egypt. Data analysis confirmed the presence of antibodies against C. abortus in 93 out of 675 sheep. The logistic regression model was fitted to identify the associated risk factors with C. abortus infection. The results revealed that C. abortus increased significantly in ewes (OR = 4.04, 95 %CI: 1.44−11.28) during autumn season (OR = 3.6, 95 %CI: 1.64–8.28), in ewes with a history of abortion (OR = 1.4, 95 %CI: 0.87–2.50) and in farm where no lambing pen (OR = 2.2, 95 %CI: 1.30–3.94) or abscence of post abortion measures (OR = 1.96, 95%CI: 1.23-3.12). In addition, age, flock size and exchange of breeding ram had no significant effect on prevalence of chlamydiosis. Also, PCR assay was confirmed presence of C. abortus as accusative pathogen in aborted ewe and the genetic characterization of Egyptian C. abortus strain revealed 100 % identity with another strain from Iraq. A control program should be applied to reduce economic losses and risk of human infection.  相似文献