泥胡菜水提物的体外抗菌作用试验     

隆雪明  游思湘  刘湘新  扶亚祥 《动物医学进展》2007,28(11):37-40

采用试管预试法和圆形滤纸层析法对泥胡菜的化学成分进行分析,结果表明,泥胡菜含有生物碱、有机酸、蛋白质、多肽、氨基酸、酚性成分、鞣质、糖类等化学成分;用纸片法和试管法对泥胡菜水提取液进行体外抗菌作用试验,结果表明,泥胡菜水提取液对金黄色葡萄球菌、巴氏杆菌、链球菌、沙门菌、大肠埃希菌都有较强的抗菌作用.抑菌圈直径分别为19.66 mm±0.53 mm、13.45 mm±0.21 mm、15.84 mm±1.23 mm、12.78 mm±0.34 mm、12.43 mm±0.52 mm;泥胡菜水提取液对金黄色葡萄球菌、巴氏杆菌、链球菌、沙门菌、大肠埃希菌的最低抑菌浓度(MIC)分别为0.063、0.125、0.25、0.125、0.25 g/mL.最低杀菌浓度(MBC)分别为0.063、0.25、0.5、0.125、0.25 g/mL.  相似文献   

青檀的综合开发利用研究   总被引：2，自引：0，他引：2  

李金昌  王秀滨 《中国水土保持》1996,(5):37-38

青檀的综合开发利用研究李金昌，王秀滨，邢示辉（安徽省泾县水电局，２４２５０２）青檀耐旱、耐瘠、适应性强，在黄红壤侵蚀区生长良好，其根系发达，固土效果显著，树皮是生产宣纸的主要原料，经济价值高，枝干可用于细木加工，枝条尾稍可作薪柴。栽培实践证明，在南方...  相似文献   

Defining the validity of a biochemical index of soil quality   总被引：2，自引：0，他引：2  

M. C. Leirós  C. Trasar-Cepeda  F. García-Fernández  F. Gil-Sotres 《Biology and Fertility of Soils》1999,30(1-2):140-146

 The native soils of Galicia (NW Spain) exhibit a biochemical equilibrium such that total soil N is a function of five biochemical and microbiological parameters: microbial biomass C, mineralized N, phosphomonoesterase, β-glucosidase and urease activities. To investigate whether the ratio of the total N calculated from biochemical soil properties (Nc) and the total N as measured by the Kjeldahl method (Nk; Nc/Nk) can be used as an index of soil quality, we determined these variables and consequently the ratio in three kinds of disturbed soils: an artificially Cu-contaminated soil, two lignite mine soils, and a number of arable soils. In none of the studied soils did the individual biochemical parameters respond consistently to the factors influencing soil quality, but in all cases soil degradation was reflected by the Nc/Nk value, which differed more or less markedly from 100%. Nc/Nk can therefore be used for the rapid evaluation of soil degradation, since it distinguishes among biochemically balanced soils, soils in a transient state of high microbiological and biochemical activity and degraded soils. It can also serve as a reliable basis for the rapid calculation of the "ecological dose" (ED₅₀) of soil pollutants. The use of Nc/Nk as an objective index of the biochemical quality of soils is recommended. Received: 20 December 1998  相似文献   

淹水土壤中久效磷的降解及消失动态     

王寿祥  刘义宁 《核农学报》1990,4(1):52-56

本试验在实验室模拟土壤淹水条件下,研究微生物对久效磷残留及降解的影响。结果表明:在未经灭菌的土壤中,久效磷及其降解物的消失比在灭菌土壤中快,相应的回归方程为C_WT=5.4502e~(-0.0254)t,C_YT=5.8509—0.0423t,残留半衰期分别为27.3和69.2天。久效磷在淹水土壤中降解为甲醇可抽提态和不可抽提态残留物,对甲醇可抽提态残留物经TLC和红外光谱分析表明,其降解物为磷酸三酯、N-去甲基久效磷、O,O-二甲基乙烯基磷酸酯,0-去甲基久效磷,以及O,C-去甲基久效磷,但主要为磷酸三酯,其含量由母液中的5％增加到28—83％,这种降解过程,在未灭菌土壤中比在灭菌土壤中快。  相似文献   

不同利用方式下红壤内在性质的演化   总被引：7，自引：0，他引：7  

陈永强  俞劲炎 《土壤通报》2004,35(2):149-151

对六个不同利用方式的红壤退化试验小区的理化、生物学性质进行了分析,结果表明:随着植被的退化,水土流失加剧,土壤容重增加,孔度减小,结构性变差;从常绿阔叶林→草地→裸露地,有机质、全氮、土壤微生物量及其酶活性也都随之下降,而CEC含量和多酚氧化酶变化却不稳定。  相似文献   

紫色土退化旱地的肥力恢复与重建   总被引：38，自引：1，他引：38  

朱波  陈实  游祥  彭奎  张先婉 《土壤学报》2002,39(5):743-749

常规耕作的水土流失造成紫色土坡耕地土层浅薄化、土壤养分贫瘠化、土壤干旱化及土壤结构劣化等土壤肥力退化问题。 1 984～ 1 996年长期田间试验结果表明 ,聚土免耕通过垄沟网状结构可保持水土 ,其多年平均径流与土壤侵蚀模数分别为 65 7.7m3hm- 2 和5 30 .0tkm- 2 ,而常规耕作的相应值分别为 1 75 4 .0m3hm- 2 和 31 2 2 .0tkm- 2 ,通过聚土与改土结合 ,聚土免耕活土层厚度平均增加 1 1 .8cm ,聚土免耕耕作技术还通过垄沟强化培肥降低土壤容重 ,增加土壤有机质及N、P、K含量 ,改善土壤理化性状 ,恢复土壤肥力。聚土免耕作物产量比平作高 1 5 %～ 30 %。 1 996～ 1 998年秸秆覆盖实验结果表明 ,秸秆覆盖增加土壤有机质和养分含量 ,改善并维持土壤结构。聚土免耕与秸秆覆盖结合有利于土壤养分活化、土壤结构形成与维护 ,是紫色土退化旱地肥力恢复与重建的关键技术。  相似文献   

低丘红壤旱地水分问题及其解决途径研究进展   总被引：10，自引：0，他引：10  

李成亮  何圆球 《土壤通报》2002,33(4):306-309

阐述了低丘红壤旱地的季节性干旱和土壤物理性退化等导致的水分问题 ,总结了一些实际可行的减缓水分问题的有效措施 ,如 :农林复合生态系统的构建、植被覆盖、合理轮作、以肥调水、灌溉系统和改良土壤结构等。并在回顾有关研究的基础上 ,对低丘红壤区水分问题的研究和解决途径提出展望  相似文献   

金沙江干热河谷区退化土壤结构的分形特征研究   总被引：25，自引：5，他引：25  

宫阿都  何毓蓉 《水土保持学报》2001,15(3):112-115

分形理论是近年来兴起的一种新的研究工具。分析结果表明 :土壤粒径分布的分形维数能客观地反映退化土壤的结构状况和退化程度 ,可作为退化土壤结构评价的一项综合性定量指标。退化土壤的分形维数与土壤颗粒组成和微团聚体组成之间存在着明显的相关性。土壤中 <0 .0 0 1mm粘粒含量越高 ,>0 .2 5 mm团粒含量越低 ,则分形维数越高 ,结构性越差。对于同一土壤退化系列 ,分形维数越高 ,土壤退化越严重。  相似文献   

中药有效成分对小鼠2-细胞胚胎体外发育的作用     

高建明  孙玉成  陈武  于同泉  穆祥  杨柳  路萍 《广西农业生物科学》2006,25(Z1):203-204

如何获得更多具有活力的可操作的桑椹胚、囊胚一直是胚胎体外培养的研究热点。近二十年来,虽经采用改进培养液成分、改善培养条件、建立体细胞与胚胎共培养体系和添加生长因子、细胞因子等手段,胚胎质量有所提高,但体外受精卵的囊胚发育率仍远低于体内受精,与体内受精囊胚相比,体外受精囊胚的细胞总数和内细胞团细胞数量明显减少,胚胎移植妊娠率和产仔率较低,这些问题均严重影响了胚胎工厂化生产和胚胎产业化的发展,以及育种工作的进程。因此,探索开辟胚胎体外培养生长发育的新途径,提高其发育率和移植妊娠率显得尤为必要。目前,国内外有关中草药有效成分对体外胚胎发育作用的研究甚少。本研究以ICR小鼠2-细胞胚胎为试验材料,将中药有效成分黄芩苷(Baicalin,Bai)、川芎嗪(Ligustrazine,Lig)和小檗碱(Berberine,BR)添加于mCZB基础液中,并以添加双抗的mCZB基础液为试验对照组,比较其对胚胎体外发育的影响。结果表明,体外培养120 h的胚胎孵化率,Bai组(80.6%)、Lig组(78.3%)及BR组(75.9%)极显著高于对照组(51.8%)(P<0.01)。孵化胚胎细胞计数,BR组、Lig组和Bai组(分别为87.2±8.6、83.9±7.7和81.9±6.2),与对照组(77.4±5.6)差异极显著(P<0.01)。说明3种中药有效成分均能显著促进小鼠早期胚胎的体外发育及胚胎细胞增殖。为进一步探讨Bai、Lig、BR对胚胎体外培养发育的效果和验证其胚胎质量,将不同组别培养至桑椹、囊胚期的胚胎进行两种程序常规冷冻,解冻后继续培养,观察其冷冻效果,并将各组解冻后发育至囊胚期胚胎移植。结果表明:冷冻解冻桑椹胚体外培养8~14 h囊胚发育率,程序一中Bai组(65.8%)和Lig组(81.1%)分别显著(P<0.05)和极显著(P<0.01)地高于对照组(48.6%)和BR组(46.7%);程序二中Bai组(80.6%)、Lig组(78.3%)极显著高于对照组(55.2%)和BR组(47.6%)(P<0.01)。冷冻解冻囊胚体外培养6~8 h囊胚发育率,程序一中各组间无显著差异(P>0.05);程序二中,中药有效成分各组间差异不显著(P>0.05),但均显著高于对照组(P<0.05)。两种冷冻程序效果差异不显著(P>0.05)。移植妊娠率和产仔率,Bai组(56.3%,63.3%)、Lig组(52.6%,67.3%)和BR组(55.0%,60.5%)均高于对照组(46.2%,52.8%)。说明3种中药有效成分对于提高小鼠2-细胞胚胎体外发育质量具有一定促进作用,并有利于提高冷冻解冻后移植胚胎的发育潜力。  相似文献   

Occurrence of Polycyclic Musks in Sewage Sludge and their Behaviour in Soils and Plants. Part 1: Behaviour of Polycyclic Musks in Sewage Sludge of Different Treatment Plants in Summer and Winter (5 pp)     

Josef Müller  Walter Böhmer 《Journal of Soils and Sediments》2006,6(4):231-235

Background, Aim and Scope   Part 1: Behaviour of Polycyclic Musks in Sewage Sludge of Different Treatment Plants in Summer and Winter Part 2: Investigation of Polycyclic Musks in Soils and Plants -  Preamble. In Part 1 of the study, screening tests were performed to investigate the occurrence of PCMs in sewage sludges. For a preliminary risk assessment, further information is needed about their behaviour in the terrestrial environment. Hence, Part 2 examined the adsorption of PCMs to soil, their dissipation and leaching in soil and their uptake by plants. Background, Aim and Scope   Polycyclic Musks (PCMs) enter the environment via the waste water system. Because of their persistence, they can accumulate in different matrices like sewage sludge or biota. By the use of sewage sludge as a fertilizer, PCMs are transferred to agricultural soils. Therefore, in Part 1 of the study, screening tests were performed to investigate the occurrence of PCMs in sewage sludge. For a preliminary risk assessment, further information is needed about their behaviour in the terrestrial environment. Hence, Part 2 of the study examined the adsorption of PCMs to soil, their dissipation and leaching in soil, and their uptake by plants. Materials and Methods: In the screening study, samples of activated sewage sludge were taken both in summer and in winter at 21 treatment plants. In order to get an overview of the contamination situation, sampling covered different types of treatment plants (in rural, urban, industrial areas). Analytical methods for the determination of HHCB, AHTN, ADBI, ATTN, AHDI and ATII in the sludge samples were developed and applied. Results: The analytical screening of PCMs showed their presence in activated and dried sewage sludge samples. HHCB and AHTN represented about 95% of the PCMs investigated. Their concentrations in the activated sludge samples varied between 2.9 and 10.4 mg/kg dry mass (dm) and 1.1 to 4.2 mg/kg dm, respectively. Although different types of sewage treatment plants were investigated, similar PCM levels were found, showing the widespread input of these compounds into domestic waste water. Discussion: PCM concentrations in activated sludge varied widely. The variation drops substantially when concentrations are related to the varying dry mass. In dehydrated sludge, PCM concentrations were up to 24 mg/kg dm for HHCB and up to 6.9 mg/kg dm for AHTN. These high values are comparable to those obtained in other investigations analysing PCMs. If the degradation of organic mass during anaerobic decomposition is included in the evaluation, the figures obtained are comparable to those for activated sludge. Elimination in sewage sludge was higher in summer than in winter. Therefore, the contamination of the sludges in winter reached higher levels compared to the summer. Conclusions: The results show that PCMs are widespread contaminants in sewage sludge. Recommendations and Perspectives: PCM should be considered in a risk assessment as potential contaminants of sewage sludge destined for agricultural use. Due to the high PCM levels in sewage sludge, further investigations into the degradation and elimination behaviour in sewage sludge have to be carried out, including that involving PCM metabolites such as lactone derivatives.  相似文献