2株桑树内生菌发酵产物的降血糖作用     

叶晶晶  周晓玲  张升祥  孙凌云  赵春晓  王桂花  张进  李娟  殷浩  崔为正 《蚕业科学》2011,37(6):1046-1052

通过体外α-葡萄糖苷酶抑制试验和糖尿病模型小鼠动物试验,检验桑树内生菌菌株Stenotrophomonas maltophilia Y1和Micrococcus luteus Y2发酵产物的降血糖作用。结果表明,Y1和Y2菌株发酵液提取物对α-葡萄糖苷酶均具有显著的体外抑制作用,并呈现一定的剂量依赖性,半数抑制浓度(IC50)分别为2 307.56 mg/L和111.58 mg/L,在试验最大剂量下的抑制率分别达到59.17%和69.21%,均高于对照药物拜糖平(抑制率为53.06%)。动物试验表明,饲喂Y1菌株发酵液浓缩产物的糖尿病模型小鼠的空腹血糖(FBG)值和餐后血糖(PBG)值均低于模型组,分别存在显著和极显著差异,而对正常小鼠的血糖无明显影响,也未表现出毒副作用;调查饲喂发酵产物后糖尿病模型小鼠的体质量、进食量、饮水量、排尿量等指标,均得到明显改善。试验结果显示,2株桑树内生菌菌株的发酵液提取物均表现出与1-脱氧野尻霉素(DNJ)相同的体外抑制α-葡萄糖苷酶活性的作用,Y1菌株发酵产物表现出与DNJ相同的降低糖尿病模型小鼠血糖及改善糖尿病症状的作用特点。  相似文献   

油菜种子内生菌的检测及杀菌消毒处理方法   总被引：2，自引：0，他引：2  

张志元  罗永兰  官春云 《湖北农业科学》2005,(1):52-55

采用组织分离法检测油菜种子内生菌的带菌率和栖息部位,并进行杀灭内生菌高效杀菌剂的筛选。结果表明:油菜各品种带菌率都较高,供试的8份材料中,带菌率最低的是品系“084”,其菌落出现频率也达70.8%,带菌率最高的为甘油5号,菌落出现频率高达100%。油菜种子的内生菌群主要为Alternariabrassicae(Berk)Sacc.、A.brassicola(Sehw)Witts、Seclerotiniasclerotiorum(Lid)Barg等和多种内生细菌。油菜内生真菌既可栖息在种皮,也可栖息在胚中,而内生细菌则只栖息于胚中。采用不同杀菌剂37℃下浸种7h,发现500倍50%地菌杀净可湿性粉剂对油菜种子内生菌有很强的杀菌作用。  相似文献   

深色有隔内生真菌S12菌株遗传转化体系的建立及GFP标记菌株的获得     

赵楠  闫思远  裴瑞瑞 《西北农林科技大学学报(自然科学版)》2024,52(4):127-135

【目的】建立深色有隔内生真菌（dark septate endophyte,DSE）的遗传转化体系,获得绿色荧光蛋白(GFP)标记的DSE转化子,为研究DSE在植物根系的侵染定殖行为和侵染定殖规律奠定基础。【方法】以前期从健康枸杞根系分离的1株DSE菌株枝状枝孢菌（Cladosporium cladosporioides）S12菌株为供试材料,确定潮霉素B对其的最低抑制质量浓度,然后研究菌龄（13,15,17,19和21 h）、酶解时间（1.0,1.5,2.0,2.5,3.0和3.5 h）、崩溃酶质量浓度（10,15,20,25,30和35 mg/mL）和渗透压稳定剂种类（NaCl、KCl、CaCl2和MgSO4）对S12菌株原生质体制备的影响。采用聚乙二醇（PEG）介导原生质体转化法将PDL2质粒（含hph和gfp基因）转入S12菌株,对所获转化子进行表型、GFP荧光、PCR及拮抗活性（以尖孢镰刀菌枸杞专化型Fusarium oxysporum f. sp.Lycium barbarum LR-1菌株为靶标菌）检测,筛选与野生型S12菌株无明显差异且带有GFP标记的转化子。【结果】S12菌株在YPD液体培养基中培养17 h,以0.7 mol/L NaCl为稳渗剂,用30 mg/mL的崩溃酶酶解2.5 h,原生质体制备数量最多,为4.53×10⁶ mL^-1。通过PEG介导,将PDL2质粒（含hph和gfp基因）转入S12菌株,可得47株转化子,转化效率2.35株/μg,从中筛选出10株生长速度和产孢量与野生型S12菌株无明显差异的转化子。荧光观察、PCR和拮抗活性检测结果表明,外源基因已成功整合到S12菌株中,成功建立了S12菌株的遗传转化体系,并获得了GFP标记菌株,有6株转化子对LR-1菌株的抑菌率与野生型S12菌株无明显差异。【结论】成功建立了遗传稳定性良好的S12菌株遗传转化体系,筛选出了6株抑菌效果与野生型菌株相当的转化子。  相似文献   

Endophyte Effects on Nutrient Acquisition in Tall Fescue Grown in Andisols     

M. Hasinur Rahman  S. Saiga 《Journal of plant nutrition》2013,36(12):2141-2158

ABSTRACT

An experiment was conducted to evaluate the effect of endophyte Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon, and Hanlin on the mineral content of tall fescue (Lolium arundinaceum Schreb., SJ Darbyshire) ecotypes (Fukaura, Koiwai, and Showa) grown on two Andisols (Black Andisol and Red Andisol) with different fertilities. Black Andisol, with a naturally low content of phosphorus, was high in other nutrients, while Red Andisol, with a naturally high content of phosphorus, was low in other nutrients. Shoot dry weight was significantly higher in endophyte-infected (E+) than endophyte-free (E?) plants grown in Black Andisol. On the other hand, in Red Andisol, only Fukaura showed higher shoot dry matter production in E+ plants as compared to E- plants. In general, greater concentrations of phosphorus and manganese were found in endophyte-infected (E+) plants than non-infected (E?) plants. Accumulation of all nutrients in E+ plants was remarkably higher than in E? plants. Excluding the plant ecotype effect, the nutrient concentration and accumulation pattern for plants in the two soils was different in E+ and E? plants. Plants growing in Black Andisol had greater concentrations of phosphorus (P), copper (Cu), and zinc (Zn) in E+ plants than those of E? plants whereas for plants in Red Andisol the reverse was true. Significantly higher concentration of manganese (Mn) was found in E+ plants irrespective of soils. Regardless of endophyte and ecotypes, plants grown in Black Andisol showed significantly higher values for both nutrient concentration and accumulation than those in Red Andisol. Apart from the plant ecotype Showa, all other ecotypes had the greatest accumulation and concentration of phosphorus in E+ plants. In two types of soil and among the three plant ecotypes, endophyte infection had different effects on nutrients acquisition. Giving emphasis on nutrient accumulation, endophyte played an overall negative role on plants grown in Red Andisol. There may be a metabolic cost of harboring endophytes offset its benefit in Red Andisol. Since the same species of endophyte infected all three ecotypes, our results show that the host/endophyte interaction depends on plant ecotype as well as the ability of the soil to supply nutrients.  相似文献   

内生短短芽胞杆菌011菌发酵滤液抑菌活性研究   总被引：2，自引：0，他引：2  

王宇婷  易有金  夏菠  杨建奎  曾静 《植物保护》2013,39(4):29-33

为明确短短芽胞杆菌011菌株在植物病害生物防治中的应用潜力,采用平皿打孔法测定011菌发酵滤液抗菌谱及其活性稳定性;采用孢子萌发法测定011菌发酵滤液对番茄早疫病菌最低抑菌浓度MIC及EC50;并显微观察其对番茄早疫病菌菌丝及孢子抑制作用.结果表明:011菌发酵滤液对8种植物病原真菌均有抑制作用,其中对番茄早疫病菌抑制作用较强,抑菌带宽度为5.5 mm;对番茄早疫病菌菌丝及孢子均有致畸作用;对番茄早疫病菌最低抑菌浓度MIC为30％,有效抑菌中浓度EC50为9.64％;其活性具有较好的热稳定性(100℃处理1h活性保持在95％0),耐酸性及抗紫外线(pH2.0处理及紫外20 W,30 cm照射120 min,活性保持不变).  相似文献   

钦州湾红树植物根部内生真菌初步研究   总被引：8，自引：0，他引：8  

王桂文  李海鹰 《广西林业科学》2003,32(3):121-124

通过多年对钦州湾区域不同位点的红树林根际取样研究，发现暗色有隔内生真菌和丛枝菌根真菌在红树植物的根系中普遍存在。报道这两类真菌在红树植物根部侵染情况的初步观察结果，并分析讨论红树林根部真菌存在的可能性和可能作用。  相似文献   

带内生真菌的野大麦草粉对3种草坪草种子萌发及生长的影响     

脱浩东  杨松  张兴旭  李春杰  南志标 《草业科学》2011,28(12):2107-2111

以多年生黑麦草(Lolium perenne)、高羊茅(Festuca arundinacea)和草地早熟禾(Poa pratensis)为研究对象,用种子萌发法和盆栽法测定了野大麦(Hordeum brevisubulatum)内生真菌(Neotyphodium)对这3种草坪草种子萌发及生长的影响。结果表明,在种子萌发试验中,野大麦草粉对3种草坪草的生长均有一定的抑制作用,而且内生真菌显著增强了野大麦草粉对多年生黑麦草苗长、须根数和对草地早熟禾苗长的抑制作用（P<0.05）;显著降低了野大麦草粉对多年生黑麦草和高羊茅的发芽率及高羊茅苗长的抑制作用（P<0.05）。在盆栽试验中,野大麦草粉对3种草坪草的生长均有一定的促进作用,而且内生真菌显著增强了野大麦草粉对高羊茅和草地早熟禾的苗长和发芽率的促进作用（P<0.05）。  相似文献   

Biomass and tiller growth responses to competition between Ky31 and MaxQ Festuca arundinacea cultivars and response of Ky31 to exogenously applied liquid preparation of Neotyphodium coenophialum under glasshouse conditions     

S. M. Mersch  A. B. Cahoon 《Grass and Forage Science》2012,67(2):299-304

Tall fescue (Festuca arundinacea) forms a mutualistic symbiosis with the fungal endophyte Neotyphodium coenophialum which produces ergot alkaloids toxic to grazing animals. One approach to reduce pathological effects has been to introduce an exotic endophyte that does not produce toxic compounds yet still confers host‐plant benefits. We measured biomass accumulation and competition between two tall fescue cultivars with different endophytes grown in close proximity and tested if compounds from endophytes can diffuse from one plant to another. Ky31 (tall fescue with natural endophyte) and MaxQ (tall fescue with introduced exotic endophyte) were grown separately and together in a pot experiment under greenhouse conditions. The cultivars had comparable biomass production in monoculture, but when grown in mixed‐culture MaxQ had a significantly lower tiller number (2.92 ± 0.15 tillers/plant) and size (0.048 g ± 0.003) than Ky31 (4.06 ± 0.16 tillers/plant) (0.072 ± 0.002 g). Aqueous endophyte preparations were made and applied topically to E+ and E‐ Ky31. E‐ plants receiving doses of this preparation produced significantly more tillers (3 ± 0.18 tillers/plant) and larger tillers (0.074 ± 0.006 g) than untreated control plants (2.2 ± 0.13 tillers/plant) (0.0428 ± 0.0072 g). These observations suggested that a diffusible compound might induce a growth benefit conferred by the tall fescue endophyte.  相似文献   

披碱草内生真菌共生体中麦角生物碱的组织分布与季节动态     

徐瑞  南志标  周雁飞  李春杰 《草业学报》2012,21(3):84-92

采用高效液相色谱(HPLC)检测了2009年4,6,8和10月采集的雾灵山、沁源、五台山和百望山等4个侵染了Neotyphodium属内生真菌的披碱草种群的叶片、茎、叶鞘和根以及10月采集的种子中2种麦角生物碱(麦角酰胺和麦角新碱)的含量。结果表明,在4,6和8月,4个披碱草种群的麦角酰胺主要分布在叶片(5.037~ 30.037 mg/kg)和叶鞘(6.259~22.255 mg/kg),10月,麦角酰胺主要集中在种子(5.045~8.971 mg/kg)和叶片(3.119~6.658 mg/kg);各种群的叶片、茎秆和叶鞘中麦角酰胺在4或6月含量(13.831~30.037 mg/kg)最高,8或10月含量(3.119~6.658 mg/kg)显著减小(P<0.05),根中8月麦角酰胺(0.552~0.576 mg/kg)显著低于其他月份(P<0.05)。麦角新碱含量的分布与麦角酰胺相似,4,6和8月主要分布于叶片(2.206~8.226 mg/kg)和叶鞘(0.268~5.490 mg/kg)中,10月主要集中于种子(1.647~5.664 mg/kg)和叶片(1.353~2.251 mg/kg)中;各种群的叶片、茎秆和叶鞘中麦角新碱在4或6月(5.102~8.226 mg/kg)最高,8或10月含量(1.353~2.251 mg/kg)显著减小(P<0.05),根中麦角新碱在8月含量(0.128~0.283 mg/kg)低于其他月份。不同生长时期,4个披碱草种群整株中麦角酰胺和麦角新碱的含量均不同。麦角生物碱的总含量中麦角酰胺显著高于麦角新碱(P<0.05),麦角酰胺和麦角新碱呈极显著线性正相关(P<0.01)关系,相关系数为r=0.827。  相似文献   

短短芽胞杆菌对植物病原真菌的抑菌活性和抑菌机理     

周金伟  周红丽  易有金  柏连阳  李高阳 《植物保护学报》2016,43(4):600-607

为探讨短短芽胞杆菌菌株011对植物病原真菌的防控效果,采用平皿打孔法测定菌株011粗提液对14种植物病原真菌的抑制作用,并以番茄早疫病为研究对象,研究了其抑菌机理。结果显示,菌株011粗提液对14种病原真菌均有抑菌活性,其中对番茄早疫病菌的抑菌活性最强,抑菌率达45.9%;菌株011粗提液能导致番茄早疫病菌菌丝生长异常,抑制孢子萌发;对番茄早疫病菌菌丝生长的EC50为71.4 mg/m L,对孢子萌发的EC50和MIC分别为0.12 mg/m L和0.8 mg/m L;该粗提液能引起番茄早疫病菌菌丝的脂质过氧化程度和细胞内过氧化氢含量上升,菌丝细胞膜的蛋白质合成量下降,当粗提液浓度高于50 mg/m L时,菌丝细胞膜的麦角甾醇合成量下降;该粗提液还能降低番茄早疫病菌胞外果胶酶和纤维素酶的活性。研究表明,菌株011粗提液可作为生防制剂应用于植物病原真菌的防治。  相似文献