鸭肠炎病毒UL41、UL42基因的克隆与分析     

陈普成  柳金雄  曾青华  姜永萍  田国彬  陈化兰 《中国预防兽医学报》2009,31(12)

依据GenBank登录的鸭肠炎病毒(DEV)核苷酸序列设计引物,利用长片段PCR技术扩增了DEV基因组UL36与UL43基因之间的未知序列,扩增所得片段长度约为15 kb.经EcoRV单酶切,将其中的3.9 kb片段克隆到pUC18中.序列分析表明该3.9 kb EcoR V片段含有2个完整的转录方向相反的与单纯疱疹病毒(HSV)UL41和UL42基因同源的ORF,命名为DEV UL41和ULA2基因.通过氨基酸序列比对发现:DEV UL41基因含有5个高度保守位点,而UL42含有2个,进化树分析表明DEV与疱疹病毒科a疱疹病毒亚科的马立克病毒、火鸡疱疹病毒的进化关系非常相近,为DEV的分类提供了参考依据.  相似文献   

猪增生性肠炎的诊断及防治措施     

王志华  张福国 《北京农业》2012,(3):103

猪增生性炎是一种常见的接触性肠道传染病,对猪增生性肠炎的诊断及防治措施进行了阐述。  相似文献   

鸭病毒性肠炎病毒致鸭胚成纤维细胞发生凋亡作用的研究     

郭宇飞  程安春  汪铭书  贾仁勇  文明  周伟光  陈孝跃 《中国家禽》2007,29(14):9-11

研究通过HE染色镜检发现,鸭病毒性肠炎病毒CH强毒株(DEV-CHv)可致鸭胚成纤维细胞(DEF)出现染色质颗粒化、细胞核变形等显著凋亡特征;TUNEL试验显示,接毒组细胞核内有多量棕黄色DAB显色颗粒,表明细胞出现了凋亡现象;DNA Ladder检测表明,接毒组细胞具有分子量分别为180～200bp及其整数倍的凋亡梯带电泳图谱特征;电镜观察发现,接毒组细胞具有染色质浓缩、边移,胞浆严重空泡化,细胞核严重变形,形成凋亡小体等典型凋亡特征.上述研究结果表明鸭病毒性肠炎病毒具有显著的致鸭胚成纤维细胞发生凋亡的作用.  相似文献   

Enteritis associated with Yersinia pseudotuberculosis infection in a buffalo     

S. HUM  S. SLATTERY  SCJ LOVE 《Australian veterinary journal》1997,75(2):95-97

SUMMARY: A week after transport a 4 month old buffalo calf developed diarrhoea. Its condition gradually deteriorated and it died. Necropsy revealed acute haemorrhagic enteritis and enlarged mesenteric lymph nodes. Haemorrhages and numerous microabscesses were detected in the lamina propria of the small intestine associated with colonies of Gram negative bacteria. Yersinia pseudotuberculosis was isolated from the small intestine and from mesenteric lymph node. Enteritis caused by Y pseudotuberculosis does not appear to have been reported previously in buffalo in Australia.  相似文献   

ULTRASONOGRAPHIC APPEARANCE AND ETIOLOGY OF CORRUGATED SMALL INTESTINE     

Martha L.  Moon  DVM  MS  David S.  Biller  DVM  Laura J.  Armbrust  DVM 《Veterinary radiology & ultrasound》2003,44(2):199-203

An ultrasound pattern of corrugated, and sometimes thickened, bowel wall has been associated with pancreatitis and small intestinal lymphangiectasia. In a retrospective study, records of dogs and cats with an ultrasound diagnosis of corrugated bowel were examined for age, breed, gender, presenting complaint, abdominal radiographic results, and final diagnosis. Eighteen dogs and six cats had an ultrasound diagnosis of corrugated bowel. The final diagnosis was pancreatitis (12 of 24), peritonitis (4 of 24), enteritis (2 of 24), pancreatic neoplasia (2 of 24), diffuse abdominal neoplasia (1 of 24), lymphocytic-plasmacytic enteritis (1 of 24), thrombosis/infarction (1 of 24), and protein-losing enteropathy and acute renal failure (1 of 24). The presence of bowel wall corrugation, although a nonspecific finding, should alert one-to the possibility of pancreatitis, enteritis, peritonitis, neoplasia, or bowel wall ischemia.  相似文献   

雏鹅新型病毒性肠炎病毒的提纯和核酸链型及结构蛋白分析   总被引：6，自引：0，他引：6  

汪铭书  程安春  周毅  陈孝跃 《畜牧兽医学报》2002,33(3):276-279

本文报道雏鹅新型病毒性肠炎病毒（NGVEV）的提纯方法、核酸类型及结构蛋白分析的研究结果。采用氯仿处理－饱和硫酸铵沉淀－透析清除NH4^ 、SO4^2-柱层析分离的技术程序可获得纯将的NGVEV病毒粒子。于电镜下可观察到NGVEV具有典型的腺病毒特征。经吖啶橙染色和S1核酸酶消化鉴定NEVEV的核酸类型为双链DNA。经SDS－PAGE分析，病毒结构蛋白由15种多肽组成，即VP1（116KD）、VP2（104KD）、VP3（88KD）、VP4（68KD）、VP5（60.7KD）、VP6（54KD）、VP7（40.1KD）、VP8（36KD）、VP9（34.5KD）、VP10（24.2KD）、VP11（22KD）、VP12（20KD）、VP13（18KD）、VP14（14.2KD）和VP15（13.4KD)。其中VP4、VP7、VP8、VP9、VP14为主要结构多肽，占蛋白总量的90.2622%。  相似文献   

鸭病毒性肠炎病毒基因组异构体的研究     

柳金雄  陈普成  邬丽  姜永萍  陈化兰 《中国预防兽医学报》2011,33(8)

为研究鸭病毒性肠炎病毒(DEV)基因组异构体的存在形式,本研究在已建立的以pCC1FOS为载体的DEV疫苗株基因文库基础上对DEV全序列进行了测定,并进一步对DEV基因组的异构体进行了研究。对261个重组fosmid中的DEV基因组片段的末端序列进行测序及序列分析,初步证明了DEV基因组有3种异构体,即P型、IS型和ILS型,未发现IL型的存在。并且,以上3种异构体在基因组中所占比例分别为77.1%、8.6%和14.3%。通过对11个重组fosmid的全序列测定,进一步确认了以上3种异构体的存在。该结果为DEV的分类提供了重要实验依据。  相似文献   

猪增生性肠炎研究进展及防控措施     

张兵  吴发兴  沈文正  高睿  李军辉  王立强 《畜牧兽医杂志》2011,30(6):45-48,52

胞内劳森菌为严格细胞内寄生的无芽孢细菌,革兰氏染色阴性,抗酸染色阳性。主要感染3~20周龄猪而引起"猪增生性肠炎",临床表现慢性间歇性下痢或急性出血性下痢,排焦油样黑色粪便或血便。病理变化为小肠后段和结肠前段肠黏膜层增厚隆起,形成特征性分枝状皱折,肠腺上皮剧烈增生,形成畸形排列的分枝状肠腺。本病可进行流行病学、临床特征、病原学、病理学、血清学、分子生物学诊断,并需要与猪痢疾、猪梭菌性肠炎、仔猪副伤寒等病进行临床鉴别。本病的防控,应从猪群管理、引种、消毒、药物预防等方面考虑,接种疫苗是行之有效的措施。  相似文献   

麝鼠肠炎沙门氏菌灭活苗的制作及应用     

赵达卓  鲁承  池鹤松  孙福亮  金燮 《延边大学农学学报》2006,28(4):270-273

通过临床上分离鉴定的麝鼠肠炎沙门氏菌自制灭活苗，经过保护性试验、安全性试验、田间试验等表明，肠炎沙门氏菌灭活苗对防制麝鼠肠炎沙门氏菌病有较好的效果．  相似文献   

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage     

Li-ming Wu  Rui Guo  Lin Hui  Yong-gang Ye  Jing-mei Xiang  Chun-yun Wan  Miao Zou  Rui Ma  Xiao-zhuan Sun  Shi-jin Yang  Ding-zong Guo 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(4):475-483

Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H₂O₂) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 µM H₂O₂, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H₂O₂ treatment. H₂O₂ damaged the bovine IECs in a time-dependent manner, and this effect was attenuated by STC-1 over-expression. Furthermore, over-expression of STC-1 up-regulated Bcl-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggested that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.  相似文献