Association between intestinal lymphangiectasia and expression of inducible nitric oxide synthase in dogs with lymphoplasmacytic enteritis     

Takuro NAGAHARA  Koichi OHNO  Itsuma NAGAO  Taisuke NAKAGAWA  Yuko GOTO-KOSHINO  Masaya TSUBOI  James K. CHAMBERS  Kazuyuki UCHIDA  Hirotaka TOMIYASU  Hajime TSUJIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):20

Intestinal lymphangiectasia (IL) is a common complication in dogs. Since nitric oxide (NO) is known to relax the lymphatic vessel, we evaluated inducible NO synthase (iNOS) expression using immunohistochemistry in 13 dogs with lymphoplasmacytic enteritis (LPE) with or without IL. The duodenal iNOS expressing cells were significantly increased in dogs with IL-negative or IL-positive LPE dogs (P=0.025, P=0.007) compared with control dogs. However, there was no significant difference in iNOS expression between IL-positive and IL-negative tissues. Based on these results, there is no clear evidence for the NO overproduction in the pathogenesis of IL in dogs with LPE. Factors other than NO could, thus, contribute to IL in dogs with LPE.  相似文献   

Response to soy: T-cell-like reactivity in the intestine of Atlantic salmon, Salmo salar L     

Bakke-McKellep AM  Frøystad MK  Lilleeng E  Dapra F  Refstie S  Krogdahl A  Landsverk T 《Journal of fish diseases》2007,30(1):13-25

T-cell-mediated hypersensitivity could be central in soybean meal (SBM)-induced intestinal changes in salmon. However, tools for immunohistochemical detection of T cells have been lacking in teleosts, including Atlantic salmon. Application of a specific histochemical protocol allowed demonstration of T-cell-like reactivities in formalin-fixed, paraffin-embedded tissues using an antibody reacting to a conserved region of human CD3epsilon (Dako A0452). Characteristic staining was observed in cells of the thymus as well as distal intestine, skin, gills and spleen. These cells were negative for immunoglobulin M (IgM). Intestinal intraepithelial leucocytes were CD3epsilon positive. During the SBM-induced enteropathy, the mixed inflammatory infiltrate in the lamina propria of the distal intestine included many lymphocytes with a T-cell-like reactivity. Real-time polymerase chain reaction revealed significantly increased expression of a complex polypeptide (CD3pp), CD4 and CD8beta (P < 0.05) in the distal intestine of SBM-fed fish compared to fish meal-fed reference fish. Increased reactivity for extracellular IgM in the lamina propria and a positive material between the epithelial cells at the tips of the folds was observed, possibly due to leakage of IgM through an abrogated epithelial barrier. In conclusion, a T-cell-like response appears to be involved in this example of a food-sensitive enteropathy.  相似文献   

鸭肠炎病毒核衣壳蛋白密码子偏爱性分析     

高颖  代淑燕  张荷  程振涛  周碧君  文明 《山地农业生物学报》2011,30(2):136-140

为给鸭肠炎病毒（DEV）核衣壳蛋白（NP）基因选择良好的宿主表达系统提供参考依据,本研究运用EM-BOSS软件包CHIPS和CUSP程序对DEVNP基因进行了密码子偏爱性分析。结果显示,DEV NP基因的ENC值为51.180,编码NP蛋白的A、I等氨基酸不同密码子的使用频率存在一定的差异;从与DEV NP基因密码子使用频率比值上看,大肠杆菌22个、酵母18个、鸭12个和人20个密码子存在较大的偏爱性。由此可见,编码DEVNP蛋白密码子出现频率较均一,且酵母等真核生物与其密码子偏爱性较为接近,可作为该基因体外表达宿主的首选。  相似文献   

鸭肠炎病毒UL53截段基因的序列特性、原核表达与其抗原性检测     

张顺川  向骏  程安春  汪铭书  李丽娟  李鑫 《中国农业科学》2010,43(21)

【目的】通过选取DEV-UL53基因主要抗原域与进行DEV-UL53截段基因B细胞表位多参数预测相结合的策略,高效表达DEV-UL53截段基因,并通过Westernblot分析重组蛋白的免疫原性。【方法】通过生物信息学软件DNAStarProtean模块对UL53基因编码的gK蛋白进行主要抗原域预测,选取主要抗原域对应的UL53截段基因进行二级结构、蛋白质骨架柔性区域、表面可及性区域预测和在线预测该蛋白的亲水性及跨膜区,并对UL53截段基因进行克隆、亚克隆、原核表达与抗原性分析。【结果】UL53截段基因编码蛋白gK的B细胞表位最可能分布于Ala20—Leu25、Ser40—Met47、Leu68—Ile78、Val124—Phe128、Ile129—Tyr134、Asp176—Ile178,构建的阳性表达质粒转入BL21表达宿主菌经IPTG诱导外源基因获得了良好表达,经Westernblot分析表明该重组蛋白具有良好的抗原性。【结论】实现了UL53截段基因的高效表达,经Westernblot分析表明该重组蛋白具备良好的免疫原性,这为DEV-UL53基因及其编码的蛋白gK功能的深入研究、新型疫苗和诊断试剂的研制及开发等提供可用的试验材料。  相似文献   

Effect of Spray-Dried Plasma Form and Duration of Feeding on Broiler Performance During Natural Necrotic Enteritis Exposure     

《The Journal of Applied Poultry Research》2006,15(4):584-591

The effect of duration of feeding (continuous or discontinued after d 14) and form (granular vs. powder) of spray-dried plasma (SDP) on performance and mortality of broilers using used litter was evaluated with 240 Ross × Ross 308 male broilers (6 broilers per pen, 8 pens per treatment). Dietary treatments were control (no SDP) or SDP as powder or granular included in the pellet and fed continuously (d 0 to 35) or discontinued after d 14. During the experiment, broilers developed necrotic enteritis, and tissue cultures were positive for Escherichia coli and Salmonella, resulting in 50% mortality on control broilers. Addition of SDP to the feed improved (P < 0.05) average daily gain, feed intake, and feed efficiency for each period of the study (d 0 to 14, 15 to 28, 29 to 35, and 0 to 35). Continuous feeding of SDP improved (P < 0.05) average daily gain, feed intake, and feed efficiency from d 15 to 35 compared with broilers fed SDP to d 14. Liveability was improved (P < 0.05) in broilers consuming SDP either for 14 d or continuously throughout the experiment compared with control broilers. Spray-dried granular plasma was more effective than spray-dried powder plasma from d 0 to 14. The results of this experiment confirmed that SDP improved broiler growth rate, feed intake, feed efficiency, and minimized enteric challenge associated with necrotic enteritis with maximal protection afforded by continuous feeding. The response to SDP was independent of age of the broiler.  相似文献   

鸡溃疡性肠炎油乳剂灭活苗的研制     

贾世玉  刁有祥  付兴伦  邢仁增  马凤龙 《中国兽药杂志》1999,33(3):1-3

将大肠梭状芽胞杆菌接种于色氨酸磷酸液体培养基作厌氧培养，３６ｈ 后离心收集细菌，生理盐水稀释使菌体浓度达５×１０９ ＣＦＵ／ｍ ｌ，经甲醛溶液灭活后，与油佐剂乳化制成鸡溃疡性肠炎灭活苗。经检测证明，该疫苗安全、可靠，无毒副作用，有效免疫期可达６个月  相似文献   

鸭病毒性肠炎病毒荧光实时定量PCR检测方法的建立和应用   总被引：1，自引：0，他引：1  

《中国兽医科技》2006,36(6):444-448

  相似文献   

水貂肠炎病毒高免卵黄抗体的制备及应用   总被引：2，自引：0，他引：2  

闫新华  闫喜军  赵传芳 《中国预防兽医学报》2000,22(4):306-307

用水貂肠炎病毒免疫蛋鸡制备卵黄抗体,结果表明,经５次免疫制备的卵黄抗体,于最后一次免疫第１０天ＡＧＰ效价达１：３２,第３０天效价为１：８。该抗体较稳定。４℃保存１２０天,－２０℃保存１８０天效价不变。用该抗体治疗细小病毒肠炎病犬,治愈率达９３．９％,证实用水貂肠炎病毒制备卵黄抗体是成功的,可用于国小病毒肠炎的治疗。  相似文献   

Reintroduction of microflora from necrotic enteritis-resistant chickens reduces gross lesions and improves performance of necrotic enteritis-challenged broilers     

《The Journal of Applied Poultry Research》2017,26(3):449-457

  相似文献   

鸭瘟鸡胚化弱毒株细菌人工染色体的构建与鉴定     

张传健  许梦微  王志胜  侯继波  王继春 《江苏农业学报》2014,(5)

为了构建鸭瘟鸡胚化弱毒株DEV(atten)的细菌人工染色体(BAC),将转移载体质粒pDEVgC-pHA2DNA和DEV(atten)DNA共转染鸡胚成纤维细胞(CEF)进行重组,通过三轮挑斑纯化,以获得纯化的鸭瘟重组病毒。将纯化的鸭瘟重组病毒的DNA电转化到E.Coli DH10B中,对获得的克隆用酶切、PCR方法进行鉴定并测序。提取阳性克隆的DNA转染CEF,以拯救重组病毒。再通过将带有相同同源臂和UL44(糖蛋白质C,gC)基因的片段和阳性克隆的DNA共转染鸡胚成纤维细胞进行重组,以获得gC恢复病毒。采用0.01感染复数(MOI)感染CEF对亲本病毒、恢复病毒进行多步法生长曲线测定,比较其有无差异。结果显示:成功获得了DEV的mini-F重组病毒,命名为DEV(atten)-mini-F,获得2个阳性克隆S1、S2,将S1命名为pDEV(atten),应用pDEV(atten)拯救重组病毒获得gC恢复病毒DEV(atten)ΔgC-R,生长特性的结果显示亲本病毒与恢复病毒间无显著性差异。表明成功构建的DEV(atten)的细菌人工染色体为全基因组的感染性克隆。  相似文献