脂肪酸钙对放牧肉牛超数排卵效果及雌二醇和孕酮水平的影响     

和占星  朱芳贤  和协超  毛华明  张继才  王喆  杨世平  文际坤 《云南农业大学学报(自然科学版)》2008,23(1):73-78,83

以探讨添加脂肪酸钙对放牧肉牛(婆罗门和BMY)超数排卵效果及血中雌二醇(E2)和孕酮(P4)水平的影响为目的。将48头供体分为Ⅰ(放牧或对照组)、Ⅱ(放牧 浓缩饲料500 g/d)和Ⅲ(放牧 浓缩饲料500 g/d 脂肪酸钙100 g/d)3组,从同期发情处理开始到冲胚结束试验期32 d,并对供体牛在8个处理时段进行采血,用放射免疫分析法(R IA)分析E2和P4水平。试验结果表明:Ⅰ,Ⅱ,Ⅲ组供体牛超数排卵的排卵率分别为75.4%,74.4%和81.7%(P>0.05),受精率分别为81.4%,55.6%和93.2%(P>0.05);获胚(卵)数分别为7.0枚,6.3枚和7.3枚(P>0.05),可用胚数分别为3.9枚,2.6枚和4.7枚,Ⅲ组显著高于Ⅱ组(P<0.05);E2平均水平分别为0.57 pg/mL,0.47 pg/mL和0.63 pg/mL(P>0.05),P4平均水平分别为3.53 ng/mL,5.03 ng/mL和3.74 ng/mL,Ⅱ组高于Ⅰ,Ⅲ组(P<0.01)。表明适量添加脂肪酸钙能改善全放牧肉牛的营养、增强体质,促进排卵与受精,增加超数排卵的可用胚数;能使供体牛的E2和P4水平比对照组有所提高;添加效果冬、春季优于夏、秋季。  相似文献   

促卵泡素和促黄体生成素对儋州鸡生殖激素调控规律的研究     

王鹏  管庆丰  华蕊  蔡克奇  杨慧  侯冠彧  吴科榜 《中国畜牧兽医》2017,44(10):3029-3034

为研究促卵泡素（FSH）和促黄体生成素（LH）对儋州鸡体内其他生殖激素的调控规律,本试验通过改变FSH和LH在儋州鸡血液中的浓度,并采用双抗体一步夹心法酶联免疫吸附试验（ELISA）对处理前后儋州鸡血液中FSH、LH、催乳素（PRL）、孕酮（P）、雌二醇（E₂）的浓度进行测定。结果发现,注射外源性FSH和LH分别能提高儋州鸡血液中FSH和LH浓度;当儋州鸡血液中FSH或LH浓度显著升高时则均能引起PRL浓度显著降低（P < 0.05）,但当FSH和LH浓度同时显著升高时,PRL浓度显著升高（P < 0.05）;当儋州鸡血液中FSH浓度显著升高时,E₂及P浓度显著提升（P < 0.05）,且在高浓度LH的协同下提升幅度更大;当儋州鸡血液中LH浓度显著升高时E₂及P浓度升高但不显著（P > 0.05）。本研究结果表明,儋州鸡血液中FSH或LH浓度的提高均能降低PRL的浓度,并能不同程度的提升E₂及P的浓度,但FSH与LH浓度同时提高则能通过协同作用刺激E₂及P浓度的大幅提升,当E₂及P浓度过高时能通过刺激PRL的释放,负反馈调节血液中FSH与LH,并恢复血液中E₂及P浓度。  相似文献   

Effects of estradiol‐17β on survival,growth performance,gonadal structure and sex ratio of the tiger puffer,Takifugu rubripes (Temminck & Schlegel, 1850), fingerlings          下载免费PDF全文

Yuqin Ren  Qin Zhou  Yongxin Liu  Yufen Wang  Qinglin Wang  Xiufeng Jiang  Qinghai Yu  Hongtao Zhang 《Aquaculture Research》2018,49(4):1638-1646

Systematic research was performed to assess the survival, growth performance, gonadal structure and sex ratio during and after sex reversal induced by estradiol‐17β in tiger puffers. The fish of 20 days after hatching (dah) were immersed in different doses (0.2, 2, 20, 100 ppb) for 60 days. There were significant differences in survival rates among groups (p < .05). With the increase in the estradiol‐17β dose, the survival rates of tiger puffers decreased, revealing a linear regression relationship with log₁₀ (estradiol + 1; p < .05). The growth tendency of fish in the low‐dose group was similar to those in the control group over the same period. The growth in the 20 ppb dose group was significantly slower than in the low‐dose and control groups in fish of same age (p < .05), but was significantly faster than in the 100 ppb dose group (p < .05) after 60 days. Intersex fish were categorized into intersex I, II and III types, according to the feminization degree from low to high. Phenotypic intersex puffer fish were found in the 2, 20 and 100 ppb dose groups. Intersex ratios in 2, 20 and 100 ppb were 10%, 43.3% and 46.7%, respectively, the ratios except the ones in 2 ppb group being significantly higher than those in the control group (p < .05). Moreover, 100.0% male induction for intersex II and III types occurred in 20 and 100 ppb groups. Together, these results indicated that estradiol‐17β at a dose of 20 ppb was most effective for pseudo‐female production.  相似文献   

外源性17-β雌二醇对养殖中华鲟血液若干生化指标的影响     

姚德冬  张晓雁  罗静波  柴毅 《淡水渔业》2016,(5):14-18

通过对12尾雌性中华鲟(Acipenser sinensis)背部肌肉注射不同浓度(0、0.1、0.2、0.4 mg/kg·BW)17-β雌二醇(estradiol),并在0、60、120和180 d尾部静脉采血,测定血清中钙(Ca2+)、雌二醇(E2)、睾酮(T)、白蛋白(ALB)、卵黄蛋白原(VTG)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)以及胆碱酯酶(CHE)的含量。结果显示,注射雌二醇后,Ca2+和VTG含量均先上升后下降,E2和ALP含量不断上升,T和CHE则含量不断下降。ALT和ALB含量上下波动。此结果表明,雌二醇对养殖中华鲟卵巢发育具有一定的促进作用,但浓度过高会对其肝脏造成损伤,0.1 mg/kg·BW是较为适宜的浓度。  相似文献   

Temporal effects of estradiol (E) on luteinizing hormone-releasing hormone (LHRH) and LH release in castrated male sheep     

L.S. Lubbers  S.M. Hileman  D.E. Kuehl  S.A. Ferreira  G.L. Jackson 《Domestic animal endocrinology》1998,15(6):511-524

We tested the hypothesis that rapidly expressed inhibitory effects of estradiol (E) on luteinizing hormone (LH) release in the male are attributable, in part, to suppression of luteinizing hormone-releasing hormone (LHRH) release. Hypophyseal-portal cannulated, castrated male sheep were infused with E (15 ng/kg/hr) or vehicle. Portal and jugular blood samples were collected at 10-min intervals for 4 hr before, and for either 12 hr (E, n = 4; vehicle, n = 4) or 24 hr (E, n = 8; vehicle, n = 3) after the start of infusion. In animals sampled for 16 hr, temporal changes in both LHRH and LH were assessed. In animals sampled for 28 hr, only LH data were analyzed. Before either the 12-hr or 24-hr infusion, LHRH and/or LH mean concentrations, pulse amplitude and interpulse interval (IPI) did not differ between E- and vehicle-infused animals. In animals sampled for 16 hr, no effects of time or steroid × time interactions were detected for mean LHRH and LHRH pulse amplitude; however, both were greater (P < 0.01) in vehicle-infused than in E-infused males. LHRH IPI was unaffected by infusion. In contrast, both mean LH and LH pulse amplitude declined (P < 0.01) within 4–8 hr after the start of E infusion, whereas mean LH IPI was unaffected. In animals sampled for 28 hr, an effect of time (P < 0.01) and a steroid × time interaction (P < 0.01) was detected for mean LH, and there was an effect of time (P < 0.01) on LH pulse amplitude. Mean LH IPI was not affected. Our results show that in male sheep E rapidly reduces LH release in the absence of a detectable change in LHRH release.  相似文献   

Plasma vitellogenin levels in male common carp Cyprinus carpio and crucian carp Carassius cuvieri of Lake Kasumigaura     

TAKERU  MATSUMOTO  MAKITO  KOBAYASHI  YOSHIAKI  NIHEI  TOYOJI  KANEKO  HARUHISA  FUKADA  KAORI  HIRANO  AKIHIKO  HARA  SHUGO  WATABE 《Fisheries Science》2002,68(5):1055-1066

In order to investigate the possible influence of estrogenic environmental endocrine disruptors on the reproductive activity of fish in Lake Kasumigaura, plasma levels of vitellogenin (VTG), a biomarker of estrogen exposure, were measured in wild and cultured male common carp Cyprinus carpio and wild crucian carp Carassius cuvieri . Testicular histology and plasma steroid hormone levels were also examined. Fish were collected from June 1998 to August 1999. Plasma VTG levels in most fish examined were below a detection limit (40 ng/mL) throughout the sampling period, and a small amount of VTG (43.5 ng/mL–1680 ng/mL) compared to that in females was detected in some fish. Active spermatogenesis in the testis and increased levels of plasma sex steroids were observed in most of the fish examined. Thus, no marked influence of estrogenic chemicals was detected in the reproductive activity of male common carp and crucian carp in Lake Kasumigaura collected from the sampling sites in the present investigation.  相似文献   

Testosterone increases urinary free felinine, N-acetylfelinine and methylbutanolglutathione excretion in cats (Felis catus)     

Hendriks WH  Rutherfurd-Markwick KJ  Weidgraaf K  Ugarte C  Rogers QR 《Journal of animal physiology and animal nutrition》2008,92(1):53-62

Two days after castration, urinary free felinine plus N-acetylfelinine decreased 24% in male cats, but, by day 5, the concentration had not decreased to that routinely found in males that have been castrated for several months. In a second experiment, three groups of castrated adult male cats received different subcutaneous injections: control (carrier), testosterone, testosterone plus estradiol. A fourth group of intact adult female cats received a testosterone injection. Urine was collected and analysed for free felinine, N-acetylfelinine and 3-methylbutanolglutathione. Baseline blood testosterone and estradiol concentrations were low during the pre-period, but increased sharply after hormone injections. The concentration of all three urinary metabolites increased as a result of testosterone injections with estradiol not modulating the effect. The effect of testosterone was not gender dependent. The concentration of free felinine, N-acetylfelinine and 3-methylbutanolglutathione in the urine remained low in the placebo control group throughout the study. The relative molar contribution of free felinine to the total amount of felinine containing compounds increased due to testosterone treatment, while the contribution of 3-methylbutanolglutathione and N-acetylfelinine decreased. Testosterone increases free felinine, N-acetylfelinine and 3-methylbutanolglutathione excretion in castrated adult male and intact female cats, whereas estradiol does not modulate this effect.  相似文献   

不孕奶牛用复方缩宫素乳剂处理后外周血中雌二醇和孕酮水平的变化     

杨利国  刘荣军  王元兴 《南京农业大学学报》1998,(2)

选择患持久黄体、卵巢静止和慢性子宫内膜炎的奶牛各１０头，从每种疾病类型中各选半数牛于宫内灌注复方缩宫素乳剂，其余患牛用生理盐水处理作为对照。各组患牛于处理当天和处理后第１，３，５，７，１０，１４和２１ｄ采血，用放射免疫测定方法分析血中１７β－雌二醇（Ｅ２）和孕酮（Ｐ４）水平。结果发现，持久黄体患牛在处理后血中Ｅ２水平不断升高，Ｐ４水平逐渐降低；卵巢静止患牛在处理后血中Ｅ２和Ｐ４水平均升高；慢性子宫内膜炎患牛在处理前血中Ｅ２和Ｐ４水平介于前两种病牛之间，处理后血中Ｅ２水平逐渐升高，Ｐ４水平逐渐降低。这些结果证明，复方缩宫素乳剂具有溶解黄体和诱导发情作用  相似文献   

雌二醇对大鼠子宫内嗜酸性粒细胞数量分布的影响     

王艺娟陈耀星  李国平王子旭 剂海霞 《中国兽医科技》2008,38(2):142-145

采用放射免疫法检测了15只处于发情周期不同时期（发情前期、发情期、间情期）的Wistar雌性大鼠和25只不同处理组（假手术组、卵巢摘除组、卵巢摘除＋20μg／kg雌二醇组、卵巢摘除＋100μg／kg雌二醇组、卵巢摘除＋500μg／kg雌二醇组）的大鼠血清中雌二醇浓度，并采用瑞氏一姬姆萨染色方法计数子宫内嗜酸性粒细胞（EOS）的数量。结果显示，大鼠血清雌二醇浓度在发情期最高，间情期最低。子宫内EOS数量在发情期最高，间情期最低。卵巢摘除后，血清中雌二醇浓度降低；补充雌二醇后，血清雌二醇浓度明显升高（P〈0．05）。卵巢摘除后子宫内EOS数量也明显降低；补充雌二醇后，EOS数量显著增加（P〈0.05）。提示，随着血清雌二醇浓度的升高，子宫内EOS数量呈递增趋势，也就是说，外源性雌二醇对子宫内EOS数量有直接的影响。  相似文献   

苯甲酸雌二醇对L-谷氨酸单钠损毁弓状核雌性大鼠血脂代谢的影响     

钟平  张兆强  蔡波  刘古锋  刘锡仪 《湛江医学院学报》2009,27(1):9-10

目的观察苯甲酸雌二醇（EB）对L广谷氨酸单钠（MSG）损毁弓状核雌性大鼠体质量、脂肪湿质量及血脂代谢的影响。方法皮下注射MSG建立新生SD雌性大鼠弓状核损毁模型。造模大鼠于30日龄时分别肌肉注射EB0．24mg／kg（MSG＋EBL组）、0．48mg／kg（MSG＋EBH组）,隔天1次,共30次;停止注射后15d处死大鼠,检测体质量、脂肪湿质量、血脂、血清雌二醇（E2）。结果模型组大鼠体质量、脂肪湿质量／体质量、血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL—C）都明显升高（P〈0．05）,而E2、高密度脂蛋白胆固醇（HDL-C）显著降低（P〈0．05）。EB处理后大鼠的体质量、脂肪湿质量／体质量、TC、TG、LDL-C均显著回降（P〈0．05）,而E2、HDL—C显著回升（P〈0．05）;MSG＋EBH组与MSG＋EBI组比较,其各项指标差异均无统计学意义（P〉0．05）。结论EB对弓状核损毁的雌性大鼠血脂代谢紊乱有治疗作用。  相似文献