TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE2分泌的影响     

TCDCA对IL  刺激下AA大鼠成纤维样滑膜细胞中PGE分泌的影响 《畜牧与饲料科学》2021,42(5):1-5

[目的] 进一步探讨牛磺鹅去氧胆酸（taurochenodeoxycholic acid,TCDCA）在抗炎免疫方面的潜在调节作用。[方法] 以AA大鼠成纤维样滑膜细胞作为研究对象,采用ELISA方法检测TCDCA和IL-1β作用下AA大鼠成纤维样滑膜细胞上清液中PGE₂的含量,分析TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌情况的影响。[结果] TCDCA能够对IL-1β刺激下AA大鼠纤维样滑膜细胞PGE₂的分泌产生下调作用（P<0.05）。[结论] TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂的分泌具有抑制作用,为TCDCA在兽医临床应用提供依据。  相似文献   

Abscisic Acid May Alter the Salicylic Acid‐Related Abiotic Stress Response in Maize     

M. Pál  T. Janda  G. Szalai 《Journal of Agronomy and Crop Science》2011,197(5):368-377

The effect of 0, 0.05 or 0.1 mm abscisic acid treatment on chilling tolerance and salicylic acid‐related responses was investigated in young maize seedlings (Zea mays L., hybrid Norma). Although the pre‐treatment of maize seedlings with abscisic acid slightly decreased the chlorophyll content, it also reduced the level of chilling injury caused by 6 days of cold treatment at 5 °C. Under normal growth conditions, increased levels of bound salicylic acid and of bound ortho‐hydroxycinnamic acid were observed in the leaves during abscisic acid treatment. In the roots, abscisic acid did not affect the free and bound salicylic acid levels, but increased the amount of free and bound ortho‐hydroxycinnamic acid. The activity of glutathione‐S‐transferase increased on the 3rd day of abscisic acid treatment, whereas it did not change when followed by cold stress, compared with the control leaves. In the roots, the activities of glutathione reductase, glutathione‐S‐transferase and ascorbate peroxidase increased during the abscisic acid treatment, and those of glutathione‐S‐transferase and ascorbate peroxidase were also stimulated when abscisic acid pre‐treatment was followed by cold stress, compared with the control roots. Our results suggest that an overlap may exist between the abscisic acid‐induced cold acclimation and the salicylic acid‐related stress response.  相似文献   

5‐Aminolevulinic Acid Activates Antioxidative Defence System and Seedling Growth in Brassica napus L. under Water‐Deficit Stress     

D. Liu  Z. F. Pei  M. S. Naeem  D. F. Ming  H. B. Liu  F. Khan  W. J. Zhou 《Journal of Agronomy and Crop Science》2011,197(4):284-295

The present study assesses the effects of 5‐aminolevulinic acid (ALA, 0, 0.1, 1 and 10 mg l^?1) on the growth of oilseed rape (Brassica napus L. cv. ZS758) seedlings under water‐deficit stress induced by polyethylene glycol (PEG 6000, 0 and ?0.3 MPa). Water‐deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non‐enzymatic antioxidants (glutathione and ascorbic acid) levels. On the other hand, water‐deficit stress enhanced the malondialdehyde (MDA) content, reactive oxygen species (ROS) production, enzymatic antioxidants activities, reduced/oxidized glutathione ratio (GSH/GSSG) and reduced/oxidized ascorbic acid (ASA/DHA) ratio in seedlings. Application of ALA at lower dosages (0.1 and 1 mg l^?1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings, whereas moderately higher dosage of ALA (10 mg l^?1) hampered the growth. The study also indicated that 1 mg l^?1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water‐deficit stress. Lower dosages of ALA (0.1 and 1 mg l^?1) also enhanced GSH/GSSG and ASA/DHA as compared to the seedlings under water‐deficit stress. The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l^?1 ALA treatment under water‐deficit stress. It was also revealed that 1 mg l^?1 ALA treatment alone induced the expression of APX, CAT and GR substantially and under water‐deficit stress conditions ALA treatment could induce the expression of POD, CAT and GR to a certain degree. These results indicated that 0.1–1 mg l^?1 ALA could enhance the water‐deficit stress tolerance of oilseed seedlings through improving the biomass accumulation, maintaining a relative high ratio of GSH/GSSG and ASA/DHA, enhancing the activities of the specific antioxidant enzymes and inducing the expression of the specific antioxidant enzyme genes.  相似文献   

Effect Of Replacing Fish Meal With Blood Meal On Chemical Composition Of Supplement For Nile Tilapia (Oreochromis Niloticus)     

J. G. Kirimi  J. M. Munguti 《East African Agricultural and Forestry Journal》2017,82(1):1-9

The objective of the work was to evaluate the effect on the nutrient content of replacing fish meal (FM) with blood meal (BM) in fish supplement. Three isonitrogenous diets (35% crude protein) were formulated using FM as the main source of animal protein (BM0); 50% replacement of FM with blood meal (BM50); or 100% replacement of FM with BM (BM100). The chemical composition (ash, crude protein, crude fat and crude fibre) and amino acid composition were determined. Replacement of FM with BM did not affect the proximate composition of the diet apart from ash content which decreased with the level of substitution. Substituting fish meal with blood meal reduced the levels of methionine, lysine, isoleucine, leucine, proline, valine and increased the levels of arginine, phenylanine and alanine in the diet. Amino acid indices revealed that BM0 had more amino acids with the highest chemical scores followed by BM50 and BM100. In all the diets, methionine was the most limiting amino acid. The essential amino acid index of the diets reduced with the level of replacement of FM (0.94, 0.88 and 0.77). The study showed substitution of up to 50% FM with BM gave a useful protein diet and 100% gave almost a poor protein diet.  相似文献   

Glycine max and Glycine soja are capable of cold acclimation          下载免费PDF全文

J. Robison  N. Arora  Y. Yamasaki  M. Saito  J. Boone  B. Blacklock  S. Randall 《Journal of Agronomy and Crop Science》2017,203(6):553-561

Soybean has been considered a cold intolerant species; based largely upon seed germination and soil emergent evaluations. This study reports a distinct acquisition of cold tolerance, in seedlings, following short acclimation periods. Diversity in cold responses was assessed in eight cultivars of Glycine max and six accessions of G. soja. All varieties of soybean significantly increased in freezing tolerance following acclimation. This study indicates soybean seedlings are indeed capable of sensing cold and acquiring cold tolerance. Germination rates after cold imbibition were negatively correlated with maturity group, but positively correlated with cold acclimation potential in G. soja. Seed fatty acid composition was varied between the species, with Glycine soja accessions containing about 2‐times more linolenic acid (18:3) than G. max. Furthermore, high levels of linoleic acid (18:2) in seeds were positively correlated with germination rates following cold imbibition in G. soja only. We suggest that domestication has not impacted the overall ability of soybean to cold acclimate at the seedling stage and that there is little variation within the domesticated species for ability to cold acclimate. Thus, this brief comparative study reduces the enthusiasm for the “wild” species as an additional source of genetic diversity for cold tolerance.  相似文献   

土壤中根皮苷和根皮素对桃幼苗的影响   总被引：1，自引：0，他引：1  

张素素  徐少卓  孙申义  王嘉艳  陈学森  沈向  尹承苗  毛志泉 《园艺学报》2017,44(6):1167-1173

以一年生桃嫁接苗为试材,在盆栽条件下研究了苹果园土壤中特征酚酸类物质——根皮苷和根皮素对桃苗生物量、根系活力、根系保护性酶活性和MDA含量的影响。结果表明,根皮苷对桃幼苗的伤害显著,与对照相比,鲜质量、干质量分别降低了16.66%和21.58%,根系活力降低了33.04%,SOD、POD活性分别降低了40.80%和31.10%,MDA含量提高了116.34%。根皮素对桃苗的影响不大。高锰酸钾处理明显缓解了根皮苷对桃苗的伤害,苹果园土壤中的根皮苷含量降低了57.80%,提高了桃苗的生物量、根系活力和保护性酶活性,降低了MDA含量。综上,老龄苹果园土壤实测含量的根皮苷(0.343mg·kg~(-1))可降低桃苗的生物量,适量的高锰酸钾能缓解根皮苷的伤害作用。  相似文献   

哺乳动物Acot2基因的研究进展     

《中国畜牧杂志》2019,(10)

酰基辅酶A硫酯酶2(Acot2)是酰基辅酶A硫酯酶(Acots)家族成员之一,其将酰基辅酶A(CoA)水解成游离脂肪酸和CoA,具有维持细胞水平的游离脂肪酸和酰基CoA(游离脂肪酸的活化形式)的潜力,在脂质代谢中发挥重要作用。本文对Acot2基因的定位、结构特征、生理功能及其调控进行综述,以期对Acot2基因的进一步探究提供参考。  相似文献   

光质对番茄营养与风味品质的影响     

杨忠武  刘翼  金卓君  徐卫红 《中国农学通报》2020,36(34):134-141

以番茄为试验材料,在田间大棚设施条件下,选用白、蓝、黑、红、绿5种不同颜色的遮阳网,探讨不同光质对番茄营养与风味品质的影响。结果表明,在青熟期,黑色光质促进了总氮、蛋白氮以及非蛋白氮的积累。青熟期到转色期,白色光质的番茄有机酸总量较青熟期增加了4.57倍,蓝色和绿色光质提高了番茄的总糖和还原糖含量。从转色期到成熟期,番茄氨基酸含量大小表现为蓝色>绿色>黑色>红色>白色;黑色光质的番茄有机酸总量较转色期增加了27.8%;红色光质提高了番茄红素含量和Vc含量,降低了硝酸盐含量;白色、红色和绿色光质增加了番茄的总糖和还原糖含量。番茄主要风味物质(>30 μg/kg)为水杨酸甲酯、6-甲基-庚烯-2-酮、柠檬烯、反-2-己烯醛。红光使番茄水杨酸甲酯、6-甲基-庚烯-2-酮和己烯醛含量较白光增加了11.6%、20.2%和17.2%。在大田设施条件下,转色期到成熟期可以采用红色光质提高番茄果实营养品质和风味。  相似文献   

酸改性麦麸挂面配方的研究     

冯瑾芮  王燕  姚慧慧  吴卫国  赵传文 《中国农学通报》2020,36(22):116-122

为改善挂面制品的营养保健功能,研发谷物加工副产物的深加工产品,将经过超声波-酸解法改性处理后的麦麸粉添加到面粉中用于制作酸改性麦麸挂面。通过单因素试验及正交试验优化确定酸改性麦麸挂面的最优配方。结果表明,在面粉100 g的基础上,酸改性麦麸粉添加量为6%（相当于面粉质量的百分比）、水添加量为56%、食盐添加量为3.5%、食碱添加量为0.4%、羧甲基纤维素钠添加量为0.4%,此条件下制得的挂面品质良好,且外观、适口性较好。  相似文献   

植物脱落酸信号转导途径的上游信使     

陶怡  王盈阁  李鸿杰  李晚忱  付凤玲 《核农学报》2016,(9):1722-1730

脱落酸(ABA)是一种重要的植物激素,调节多种植物生理过程,并在植物应答逆境胁迫的信号转导中起重要作用。结构分子生物学分析发现,PYL蛋白具有结合ABA的活性中心。在非胁迫条件下,ABA水平较低,2C型蛋白磷酸酶(PP2C)与蔗糖非酵解型蛋白激酶2(SnRK2)结合,催化其去磷酸化而抑制其活性;在胁迫条件下,ABA水平升高,促进PYL与PP2C结合而抑制其磷酸酶活性,SnRK2靠自身磷酸化激活,又催化碱性亮氨酸拉链(b ZIP)、碱性螺旋-环-螺旋(b HLH)等类型转录因子,调控下游抗性相关基因的表达,也可由SnRK2直接催化下游抗性相关蛋白磷酸化。本文概述了在ABA胁迫下,PYL,PP2C和Sn PK2作用机制的相关研究进展,并建议将PYL、PP2C和SnRK2分别称做ABA信号转导途径的直接受体、第二信使和第三信使,建立玉米中复杂的脱落酸信号上淳传递网络。  相似文献