皱纹盘鲍（Haliotis discus）脓疱病的研究   总被引：19，自引：0，他引：19  

刘金屏  聂丽苹  李太武  丁明进  宋协民  赵蓉 《中国水产科学》1995,2(2):78-84

１９９３年夏季，大连水产养殖公司槽式养殖的鲍（Ｈａｌｉｏｔｉｓ）发生脓疱病，死亡率高达５０－６０％，经分离纯化得９３０１和９３０２二个菌株，镜检结果表明，两个菌株均匀极生单鞭毛的革兰氏阴性短杆形细菌。在人工感染试验中，健康鲍足肌分别注射两个菌株的菌液，感染率均高达１００％，病症与自然发病的安全相同，因此，证明９３０１和９３０２菌株即为脓疱病的病原菌，药敏试验表明，两个菌株对氯霉素，复方新诺明高度敏  相似文献   

Negligible risk associated with the movement of processed rainbow trout, Oncorhynchus mykiss (Walbaum), from an infectious haematopoietic necrosis virus (IHNV) endemic area     

S E LaPatra  W N Batts  K Overturf  G N Jones  W D Shewmaker  & J R Winton 《Journal of fish diseases》2001,24(7):399-408

  相似文献   

栉孔扇贝大规模死亡致病病原的研究   总被引：4，自引：2，他引：4  

王崇明 《水产学报》2004,28(5):547-553

对2000-2002年山东沿海6个疫区患病栉孔扇贝进行电镜观察，在消化腺、外套膜、肾和肠的结缔组织细胞和间质细胞中发现一种球形病毒．并引起相直的病理学变化。该病毒具囊膜，直径为130～170nm，核衣壳直径为90～140nm。病毒分离纯化后．观察到的病毒囊膜表面覆有长20nm的纤突。病毒在细胞质中的囊泡样结构内完成装配，其内未发现包涵体存在。从发病疫区栉孔扇贝组织中分离出病毒毒种对键康扇贝进行人工感染。结果显示，各感染实验组发病扇贝表现出与自然海区发病扇贝相同的临床症状。病毒注射组死r二率为?5％，病毒浸浴组死亡率为68．7％．灭活病毒注射组和空白对照组死亡率皆为12．5％。病毒注射、浸浴组与灭活病毒注射组、空白对照组死亡率差异显著。电镜复检结果显示，各感染实验组发病扇贝组织中分布有大量病毒粒子，与自然海区发病扇贝组织所观察到的病毒粒子在形态特征和病理学特征上完全一致。以上结果证明，病毒是导致栉孔扇贝大规模死亡的直接病原。  相似文献   

Reduction of transport stress of ayu by obligated schooling   总被引：3，自引：0，他引：3  

KEI'ICHIRO IGUCHI  FUMINARI ITO  KOGI OGAWA  NAOTO MATSUBARA  TAIGA YODO  TSUGIKO YAMASAKI 《Fisheries Science》2002,68(4):849-853

We investigated stress responses of ayu Plecoglossus altivelis under the hypothesis that overcrowding during transport can be a stressor and the stress predisposes fish to disease by decline in immunocompetence. The extent of stress response was determined under different fish densities. As inferred from the serum concentration of cortisol 1 day after the treatment, the density at more than 23 kg/m³, which was within the range of normal transporting procedures, acutely evoked intense stress responses. To examine a possible way to reduce the stress response of fish under crowding conditions, we compared two fish groups established in water tanks with circulating (whirling) and turbulent currents at the same density. The cortisol levels 1 day after the treatment were 2.5-fold higher in turbulent current than in whirling current. Fish in whirling current formed a school, while those in turbulent current aggregated with each other. Schooling of fish induced by water flow may reduce their stress responses during transport. A transport system with a directional water flow should keep fish in good condition without reducing the number of fish contained.  相似文献   

Virion glycosylation governs integrity and infectivity of infectious pancreatic necrosis virus     

Fridholm H  Everitt E 《Journal of fish diseases》2011,34(9):663-675

The possible importance of the O-linked glycosylation in virion stability and infectivity of infectious pancreatic necrosis virus (IPNV) was analysed. Enzymatic treatment with O-glycosidase of radiolabelled virions under different ionic conditions, to allow for possible alternative exposure of glycosidic enzyme cleavage sites, did not alter the specific infectivity of virions re-isolated after rate-zonal centrifugation in glycerol gradients. As an alternative method to assess the significance of carbohydrates in IPNV integrity, periodate oxidation in the presence of an aldehyde quencher was chosen. Following re-isolation of viruses, a 3-5 (10)log-unit reduction in specific infectivity was revealed and, at higher concentrations, a total disruption or virion aggregation was observed. The loss of infectivity of intact virions was not because of a lack of attachment to cells. Additionally, re-evaluation of reading values from UV-spectra of purified IPNV yielded a specific infectivity of 3 × 10(11) TCID(50)-units mg(-1) of protein and a ratio of 40 virions per TCID(50)-unit in the CHSE-214 cell system.  相似文献   

Production of polyclonal antiserum against recombinant VP28 protein and its application for the detection of white spot syndrome virus in crustaceans     

Yoganandhan K  Syed Musthaq S  Narayanan RB  Sahul Hameed AS 《Journal of fish diseases》2004,27(9):517-522

The VP28 gene of white spot syndrome virus (WSSV) was cloned into pRSET B expression vector. The VP28 protein was expressed as a protein with a 6-histidine taq in Escherichia coli GJ1158 with NaCl induction. Antiserum was raised against this recombinant-VP28 protein in rabbits and it recognized VP28 protein in naturally and experimentally WSSV-infected shrimp, marine crabs, freshwater prawns and freshwater crabs. The antiserum did not recognize any of the other known WSSV structural proteins. Various organs such as eyestalks, head muscle, gill tissue, heart tissue, haemolymph, tail tissue and appendages were found to be good materials for detection of WSSV using the antiserum and detection of WSSV was successful in experimentally infected Penaeus monodon and P. indicus at 12 and 24 h post-infection (p.i.), respectively. The antiserum was capable of detecting WSSV in 5 ng of total haemolymph protein from WSSV-infected shrimp.  相似文献   

鲤鱼单极虫病组织病理研究   总被引：1，自引：0，他引：1  

肖国华  张卫平 《河北渔业》1992,(4):14-17

本文报道了1989年以来,造成王快水库等网箱养鲤大批死亡的单极虫病,病鱼出现烂鳃、肛门红肿、腹部膨大,腹腔内有大量粘液等症状;显微观察的结果为:各组织器官发生病变,肠上皮细胞严重坏死,平滑肌纤维发生玻璃样变及解体,鳃丝充血,鳃小片融合,肝细胞核溶解、坏死。  相似文献   

多糖和益生菌对暗纹东方纯免疫功能的调节   总被引：12，自引：0，他引：12  

华雪铭 《水产学报》2006,30(3):377-382

2003年春以来，山东、辽宁等地区养殖的仿刺参出现急性口围肿胀症，其症状是口围肿胀，体表溃烂，排脏，管足附着力下降，脱落沉至池底，死亡率较高。从病参口围、体表及体壁分离出优势菌10株，经人工感染试验证实其中菌株KW21、KW22、KW23、NB13和NB14是引起仿刺参出现急性口围肿胀症的病原菌。通过16S rRNA基因序列同源性及系统发育分析表明，菌株KW21为Mahnomonas dokdonens／s；菌株KW22和菌株NB13具有相近的亲缘关系，两者均与Vibrio splendidus有较高的相似性（99％，98％）；菌株KW23为Vibrio tapetis；菌株NB14为Vibrio sp．。5株病原菌均为革兰氏阴性菌，电镜下观察菌株KW21棒杆状；菌株KW22、KW23、NB13和NB14分别为杆状、弧状、卵圆形和卵圆形，且都具有单极生鞭毛。药物敏感性试验结果表明，诺氟沙星、氧氟沙星和红霉素对5株菌均有较强的抑菌作用。Vibrio tapetis，Vibrio splendidus和Marinomonas dokdonensi作为仿刺参的病原菌属首次报道。  相似文献   

夏大豆优质高产栽培技术     

张智 《农林科学实验》2014,(11):45-46

介绍了夏大豆优质高产栽培技术,主要包括种子选择及处理、播种、田间管理、肥水运筹、病虫草害防治等内容,以供种植户参考。  相似文献   

Assessment of the potential relationship between egg quality and infectious bronchitis virus infection in Australian layer flocks     

KA Hewson  T Robertson  PA Steer  JM Devlin  AH Noormohammadi  J Ignjatovic 《Australian veterinary journal》2014,92(4):132-138

  相似文献