Preparation and Identification of N-glycolylneuraminic Acid IgY Antibody     

WU Zong-cheng  GAO Yang  HU Pan  LI Yan-song  ZHOU Yu  LIU Zeng-shan  REN Hong-lin  LU Shi-ying 《中国畜牧兽医》2016,43(3):629-636

The experiment was carried out to establish a method for preparation and identification of N-glycolylneuramic acid IgY antibody.Neu5Gc was linked to carrier proteins by carbodiimide(EDC)method.After the immunization of immunogen to laying hens,the IgY antibody was gained.Neu5Gc-IgY was analyzed and identified by ELISA.The results of UV and agarose gel electrophoresis showed that the artificial antigens of Neu5Gc were successfully synthesized.The development of the IgY antibody titer was monitored by indirect ELISA.The result showed that the IgY antibody was generated at 7th day after the first immunization.The titer was gradually increased and reached its peak (1:10 000) at 63th day after the first immunization.The high titer of the IgY antibody maintained through the whole observation period.The sensitivity of anti-Neu5AGc IgY antibody was detected by the competitive blocking ELISA.The linearity about the first chicken of the standard curve showed good,the liner regression equation was y=30.28x+16.923,R²=0.9581.The IgY antibody gained in this study laid the foundation for the establishment of indirect ELISA immunoassay method for detecting Neu5Gc in red meat,milk and tumor tissues.  相似文献   

Effects of iron glycine chelate on laying performance,antioxidant activities,serum biochemical indices,iron concentrations and transferrin mRNA expression in laying hens     

Chao Xie  Hamada A. M. Elwan  Shaaban S. Elnesr  Xinyang Dong  Jie Feng  Xiao‐ting Zou 《Journal of animal physiology and animal nutrition》2019,103(2):547-554

This study was conducted to evaluate the effects of iron glycine chelate (Fe‐Gly) on laying performance, antioxidant enzyme activities, serum biochemical indices and iron concentrations in laying hens. A total of 810 laying hens (Hy‐Line Variety White, 26 weeks old) were randomly assigned to six groups with five replicates of 27 layers. Hens in the control group received diet supplemented with 60 mg Fe/kg as FeSO₄, while hens in other five groups received the diet supplemented with 0, 20, 40, 60 and 80 mg Fe/kg from Fe‐Gly respectively. The results showed that dietary Fe‐Gly treatments significantly influenced (p < 0.01) the laying rate and egg weight of layers, compared with the control group. Concerning to CuZn‐superoxide dismutase (CuZn‐SOD) and total superoxide dismutase (T‐SOD) activity, Fe‐Gly groups (60, 80 mg Fe/kg) were promoted significantly (p < 0.01) compared with 0 mg Fe/kg group. The concentrations of Fe in serum, liver, kidney, spleen and ovary were increased significantly with the level dietary Fe‐Gly raised where Fe‐Gly groups (60, 80 mg Fe/kg) had observably higher Fe concentration than the control (p < 0.01) in serum, kidney and spleen. There was a trend that transferrin mRNA expression was decreased with the increase of Fe as Fe‐Gly in diets, and compared with the control, the expression was lower in the group fed diet with 60 mg/kg Fe as Fe‐Gly. In conclusion, Fe‐Gly (60 mg Fe/kg) had improved laying rate, egg weight, SOD enzyme activity, Fe absorption and protein synthesis in body and promoted iron metabolism in laying hens. Moreover, Fe‐Gly (40 mg/kg Fe) had the similar effect with control group. It revealed that FeSO₄ could be substituted by lower concentration of Fe‐Gly and Fe‐Gly may be superior to FeSO₄ for iron fortification to laying hens.  相似文献   

Effects of Spirulina algae as a feed supplement on nutritional value and flavour components of silkie hens eggs     

Guoshun Chen  Yu Cai  Yingyu Su  Bolan Gao  Hongbin Wu  Jiyou Cheng 《Journal of animal physiology and animal nutrition》2019,103(5):1408-1417

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罗曼褐父母代产蛋前期产蛋规律的分析   总被引：1，自引：1，他引：0  

黄炎坤  韩占兵  张亚丽 《中国畜牧兽医》2008,35(6)

对1249只健康的23周龄的罗曼褐父母代种母鸡的产蛋情况进行观察,试验期67 d(至32周龄),目的在于了解产蛋前期,蛋用种鸡产蛋性能的变化规律。试验结果表明,随鸡群日龄的增大蛋壳颜色逐渐变浅,产蛋率和种蛋合格率逐渐上升,鸡群的死淘率在开始人工授精的前2周最高,双黄蛋率随产蛋率的上升而逐渐减小,双黄蛋平均重随产蛋率上升逐渐增大,到产蛋率达最高峰时开始变小,软壳蛋率和破蛋率随鸡群日龄的增大而逐渐减小,总蛋重及种蛋平均重随鸡群日龄的增大而逐渐增大。  相似文献   

桑叶的营养价值及其在畜禽生产中的应用研究进展     

杨灿  唐小武  欧慧敏  杨海  唐青海  唐姣玉  曹丽敏 《中国畜牧兽医》2019,46(5):1388-1396

桑叶含粗蛋白质、维生素(维生素A、维生素C)、微量元素(铁、锰、铜、锌)等营养组分及黄酮类等多种活性成分,是理想的动物蛋白质资源。研究发现,在猪生产中,添加15%桑叶可能会影响育肥猪的生长,但添加适量的桑叶(6%左右)可延缓宰后肌肉pH降低,增强肌肉的抗氧化能力,同时,桑叶可提高肌肉脂肪沉积,对肌肉氨基酸和脂肪酸组成(增加不饱和脂肪酸的含量)均有影响,并改善肉的风味。在家禽生产中,添加桑叶能降低鸡肉的pH,且可通过改变肌肉脂肪酸组成改善肉品质。此外,桑叶的活性成分已被证实能提高细胞或小鼠的抗氧化能力,具有降血脂、血糖及动脉粥样硬化等作用。本文简述了桑叶的营养价值及其在猪、肉鸡、蛋鸡生产中的应用,旨在为其作为蛋白质资源进一步应用于畜禽生产提供一定参考。  相似文献   

Effects of extruded flaxseed on layer performance,nutrient retention and yolk fatty acid composition     

S. Huang  A. Mustafa 《British poultry science》2018,59(4):463-469

1. This study was conducted to determine the effects of feeding an extruded flaxseed (EF) on layer performance, apparent total tract nutrient retention (ATTR) and egg yolk fatty acid concentrations.

2. Seventy-two White Leghorn laying hens (58-week-old; three per cage) were randomly allotted to one of four dietary treatments: 0%, 7.5%, 15.0% and 22.5% of EF-supplemented diets for 8 weeks.

3. Supplementation with EF had no effect on feed intake, egg production, feed conversion ratio and egg weight. Egg components (yolk, albumen and shell percentages) were similar among treatments, except that shell percentage was greater for layers fed 22.5% EF than those fed 7.5% and 15% EF. The ATTR of dry matter and organic matter were highest for 0% and 7.5% EF, intermediate for 15% EF and lowest for 22.5% EF. Similar reductions on ATTR of crude protein and nitrogen-corrected apparent metabolisable energy were observed for layers fed 22.5% EF relative to those fed 0% or 7.5% EF.

4. Feeding EF at 7.5%, 15.0% and 22.5% of the diet markedly increased (by 92%, 198% and 271%, respectively) egg yolk concentrations of n-3 polyunsaturated fatty acid (PUFA) and reduced saturated fatty acid and n-6 PUFA concentrations.

5. It was concluded that omega-3 labelled eggs (300 mg/60 g of egg) may be produced with low (7.5% of diet) levels of dietary EF without compromising egg production parameters. However, feeding moderate to high levels of EF (i.e. 15% and 22.5% EF) may reduce total tract nutrient and energy utilisation.  相似文献   

Effects of nanocalcium carbonate on egg production performance and plasma calcium of laying hens          下载免费PDF全文

S. Ganjigohari  N. Ziaei  A. Ramzani Ghara  S. Tasharrofi 《Journal of animal physiology and animal nutrition》2018,102(1):e225-e232

This experiment was conducted to evaluate the effects of nanocalcium carbonate (NCC) instead of calcium carbonate (CC) on egg production, egg weight, egg mass, FCR, blood calcium and egg quality characteristics in laying hens. A total of 120 laying hens were used in a 10‐weeks trial, from week 23 to 33 of age. Laying hens were randomly assigned to six treatments with four replications, five hens each. The experimental treatments involved replacing 50% of the CC in the diet by decreasing amounts of NCC and were T1 Basal diet (BD) with 8.06% CC; T2 (6.045% of CC as a negative control); T3 (4.03% of CC replaced by 2.015% NCC); T4 (4.03% of CC replaced by 1.01% NCC); T5 (4.03% of CC replaced by 0.252% NCC) and T6 (4.03 of CC replaced with 0.126%NCC).Egg weight was unaffected by dietary treatments (p > .05). However, the egg production percentage and egg mass in T6 were less than that of other treatments (p < .05). The laying hens in the control group had the best average feed conversion ratio (p < .05). Also, the lowest concentration of calcium in hens’ blood was recorded for birds fed T6 (p < .05). The best egg shell quality (relative egg shell weight and egg shell weight/surface) was observed in T1 (p < .05).Collectively, our results demonstrated that NCC could replace CC at a lower inclusion level but extreme reduction of calcium concentration in diets (to 1.43% Ca in the T6 group) reduced production performance, egg quality characteristics, Tibia thickness and blood calcium of laying hens.  相似文献   

A study assessing hen and progeny performance through dam diet fortification with a Saccharomyces cerevisiae fermentation product     

《The Journal of Applied Poultry Research》2013,22(4):872-877

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Effects of lead contamination in feed on laying performance,lead retention of organs and eggs,protein metabolism,and hormone levels of laying hens     

《The Journal of Applied Poultry Research》2013,22(4):878-884

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当归补血散对高龄蛋鸡生产性能及蛋品质的影响     

李春红  董玉龙  史万玉 《中国饲料》2011,(17)

120只390日龄海兰褐蛋鸡,随机分为4组。以日粮中分别添加0.5%、1%、1.5%剂量的当归补血散饲喂Ⅰ、Ⅱ、Ⅲ组鸡群,Ⅳ组为对照组。连续给药15 d。分别观察各组每天的产蛋率、日产蛋量等生产性能指标;于第8、15、22、30天各组随机收集10枚蛋测蛋品质指标。结果:试验组Ⅱ、Ⅲ的产蛋率比对照组提高了8.80%(P<0.01)、4.99%(P<0.05);日产蛋量均与对照组差异极显著(P<0.01),分别提高了12.12%、6.82%;料蛋比与对照组相比降低了12.46%(P<0.01)、11.42%(P<0.05)。Ⅱ组的蛋形指数与对照组有显著提高(P<0.05);Ⅰ和Ⅱ组的蛋壳厚度与对照组有显著提高(P<0.05)。其余指标与对照组差异不大(P>0.05)。结论:1%剂量可以明显提高高龄蛋鸡的生产性能和蛋品质。  相似文献