人干细胞因子（SCF）5’旁侧调控序列与其全长cDNA融合克隆的构建及鉴定     

谭运年  谭文斌  彭兴华 《湛江医学院学报》2001,19(1):6-9

目的：为研究人干细胞因子(SCF)5’旁侧1．42kb区域内不同序列对全长cDNA在真核细胞中表达的调控作用，构建了SCF5’旁侧1．42kb的调控序列与其1．2kb的全长cDNA融合克隆。方法：将PCR获得的SCF5'旁侧1．42kb的调控序列与RT-PCR获得的其1．2kb的全长cDNA克隆入pGEM—T载体，筛选正确插入方向，利用合适的限制性内切酶从中切出三个片段依次亚克隆入pUC19克隆载体中。结果：获得了SCF5'旁侧1．42kb的调控序列与其1．2kb的全长cDNA并成功地构建了它们的融合克隆。结论：T-载体在克隆添加了少量具有3’→5’外切核酸酶的PCR产物中仍然有效；在稍大片段的基因克隆操作中，利用分段亚克隆的方法，避开干扰另外的酶切位点，依次分段亚克隆更为可行。  相似文献   

用科学人才观指导高校的人才队伍建设     

桂祖祥  孙福 《安徽农业大学学报》2006,(4):35-37

高等学校要做好人才队伍建设工作,就必须要准确把握科学人才观的深刻内涵,牢固树立和坚决落实科学人才观,转变传统的人才考核、培养、选拔、发展与使用机制,更新观念,改善和优化人才成长、发展空间,使各类人才的活力竞相迸发,从而实现人才强校战略。  相似文献   

林州市农村青年人力资源现状及开发途径     

王庆锋 《信阳农业高等专科学校学报》2007,17(4):79-81,118

林州市的多数农村青年的素质较以前有了很大提高,但与社会主义新农村建设的要求仍有差距。应通过全面提高农村青年的文化教育水平,广泛开展职业技能培训,鼓励进城务工青年返乡创业等途径开发农村青年人力资源。  相似文献   

Temporal variation in Lake Balaton and its fish populations   总被引：4，自引：1，他引：3  

P. Bíró 《Ecology of Freshwater Fish》1997,6(4):196-216

Abstract— Dramatic changes have occurred in Lake Balaton in the last 40–45 years. Variations in water quality have resulted in a decrease in fish and invertebrate diversity. Fish assemblages have changed in response to human-induced eutrophication, environmental perturbations, introduction of exotic fish species, over-fishing and species-habitat interactions. Fish biomass and productivity first increased during the early 1970s with enhanced primary production, then decreased with the reduction of exploitation since the late 1970s and induced intra- and interspecific competition. Previous stable populations began to oscillate, and the majority of stocks became fragile by the mid-1980s. Extinction of native fish and invasion of new alien species alternate. Alien species have transformed the food webs, causing long-lasting ecological changes. Reduced and non-selective exploitation has resulted in higher fish density and lower production rates. Recent studies have revealed differences in the carrying capacity of fish between the littoral zone and the open water areas.  相似文献   

Immunopathology of <Emphasis Type="Italic">Dirofilaria immitis</Emphasis> Infection     

Simón F  Kramer LH  Román A  Blasini W  Morchón R  Marcos-Atxutegi C  Grandi G  Genchi C 《Veterinary research communications》2007,31(2):161-171

Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts, with infections occasionally being reported in humans. Studies have shown that both dirofilarial antigens and those derived from its bacterial endosymbiont Wolbachia, interact with the host organism during canine, feline and human infections and participate in the development of the pathology and in the regulation of the host’s immune response. Both innate and acquired immune responses are observed and the development of the acquired response may depend on the host and, or on its parasitological status. This review aims at illustrating current research on the role of both D. immitis and Wolbachia, in the immunology and immunopathology of dirofilariosis.  相似文献   

As the world gets smaller, the chances of invasion grow     

Jeffrey A. McNeely 《Euphytica》2006,148(1-2):5-15

Summary While the issue of invasive alien species has important biological components, economic factors such as global trade deserve much greater attention for several reasons. First, virtually all of our planet’s ecosystems have a strong and increasing anthropogenic component that is being fed by increasing globalisation of the economy. Second, people are designing the kinds of ecosystems they find productive or congenial, incorporating species from all parts of the world through quicker and more efficient means of transportation. And third, growing travel and trade, coupled with weakening customs and quarantine controls, mean that people are both intentionally and inadvertently introducing alien species that may become invasive. The great increase in the introduction of alien species that people are importing for economic, aesthetic, accidental, or even psychological reasons is leading to more species invading native ecosystems, with disastrous results: they become invasive alien species (IAS) that have significant deleterious effects on both ecosystems and economies. This paper examines some of the important non-biological dimensions of the IAS problem, including historical, economic, cultural, linguistic, health, sociological, management, legal, military, ethical, and political dimensions. These are addressed in terms of the causes, consequences, and responses to the problem of IAS. These dimensions of IAS are fundamental, and successfully addressing the problem will call for greater collaboration between different economic sectors and among a wide range of disciplines. The Convention on Biological Diversity, the negotiations of the World Trade Organisation, and many other international agreements offer important opportunities for addressing the complex global problems of IAS through improved international cooperation.  相似文献   

Systems Analysis of Value Chain of Human Capital and Its Management Process     

ZHENG Jing-li  XIONG Zheng-fang 《保鲜与加工》2005,(5):156-158

The maintenance and gain of enterprise value have something to do with human capital. Human capital impels the realization of enterprise value through their inter-relationship, depending on given organization environment. This article systemically analyses tier value chain, paratactic value chain and network value chain, which are formed in the course of the creating value by human capital, then educes the adjustors of the management and forms according to the environment.  相似文献   

An Evaluating Method of Human Resource Value Based on Rough Set     

XIAO Zhi  LIOU Min  FENG Qi shan  ZHANG Zhi heng 《保鲜与加工》2002,(11):140-142

As the development of knowledge economic , human resource has been the core of the world economic .So searching the reasonable evaluating and managing methods becomes one of the hot issues which have been concerned by people . Traditional evaluating methods of human resource are simple and direct , but they greatly depend on people's subjective judgement and experience . In order to overcome the default , this paper puts forward the method of synthetically evaluation on rough sets, which takes advantage of the rough sets theory, the concept of information entropy and relative analysis to filter the original indexes, then to standardize the importance degree of every index and get the weight of every index. Synthetically valuation model and corresponding arithmetic are summed up. Finally the sample illuminates the validity and feasibility of this method.  相似文献   

Research on the Impact of Green Roof on Indoor Human Thermal Comfort     

GAO Xu-dong  JIN Song  QU Ya-dong 《保鲜与加工》2007,(5):44-48

An experiment is conducted on the impact of green roof on Indoor Human Thermal Comfort. The result shows that the temperatures of the building walls and indoor air fall for certain degrees, and that is good. But unfortunately, the relative humidity of the indoor air rises. Totally speaking, the whole effect is changed from feeling warm to basically acceptable. When the requirement of the indoor environment is high, it is impossible to be satisfied by exploiting green roof solely, other methods, such as using night ventilation, electric fans or air-conditioners, should be taken at the same time.  相似文献   

人肥胖基因在大肠杆菌中的表达及生物学活性检测   总被引：3，自引：0，他引：3  

昔奋攻  赵春江  李宁  吴常信 《农业生物技术学报》2006,14(1):17-21

肥胖基因(ob)编码的瘦蛋白可以反映机体脂肪含量信息,在发育、繁殖、造血及体重调节等方面具有重要功能。利用PCR方法扩增去除信号肽的人肥胖基因cDNA序列,并将位于基因5!端的CCC转变为大肠杆菌(Escherichiacoli)常用密码子CCG,扩增片段经测序证实后,克隆到原核表达载体pET5a,重组质粒转化大肠杆菌BL21(DE3),经0.1mmol/LIPTG诱导,获得分子量约为16kD的特异蛋白表达带,表达量最高占菌体蛋白总含量的55%。重组蛋白纯化后,注射昆明白小白鼠,小白鼠体重明显下降,说明纯化的重组蛋白具有生物学活性。  相似文献