Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model     

Xiao-ying He  Li-bing Ma  Xiao-ning He  Wan-tong Si  Yue-Mao Zheng 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):145-152

Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a control group. The results showed that the hTERT-bMGEs group had much higher transgenic efficiency and protein expression than the bMGEs group. Furthermore, the nontransgenic and transgenic hTERT-bMGEs were used as donor cells to evaluate the efficiency of SCNT. There were no significant differences in rates of cleavage or blastocysts or hatched blastocysts of cloned embryos from nontransgenic hTERT-bMGEs at passage 18 and 28 groups (82.8% vs. 81.9%, 28.6% vs. 24.8%, 58.6% vs. 55.3%, respectively) and the transgenic group (80.8%, 26.5% and 53.4%); however, they were significantly higher than the bMGEs group (71.2%, 12.8% and 14.8%), (p < 0.05). We confirmed that hTERT-bMGEs could serve as effective target cells for improving development of somatic cell cloned cattle embryos.  相似文献   

Predicting Acceptability of Jaguars and Pumas in the Atlantic Forest,Brazil     

Monica T. Engel  Jerry J. Vaske  Alistair J. Bath  Silvio Marchini 《Human Dimensions of Wildlife》2016,21(5):427-444

Jaguars and pumas are threatened species in Brazil’s Atlantic Forest, especially at the borders of protected areas. This article assessed the influence of emotions, attitudes, existence value, and agency credibility on acceptability of big cats among rural residents living adjacent to two protected areas in this forest. Data from self-administrated questionnaires (n = 326) indicated those with positive attitudes toward big cats (β = .28, p < .001), those who valued the existence of big cats (β = .14, p < .05), those who would feel sorrow if big cats disappeared (β = .21, p < .001), and those who considered the managing agency as credible (β = .16, p = .002) were more accepting of big cats. The model provided theoretical and practical insights into large carnivore conservation. For example, given the significance of agency credibility, a positive relationship between park authorities and residents is crucial for big cat conservation.  相似文献   

Osteopontin expression in healing wounds of horses and in human keloids          下载免费PDF全文

V. Miragliotta  A. Pirone  E. Donadio  F. Abramo  M. P. Ricciardi  C. L. Theoret 《Equine veterinary journal》2016,48(1):72-77

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Physiological but not morphological adjustments along latitudinal gradients in a human commensal species,the Eurasian tree sparrow     

Qian ZHANG  Mo LI  Yuan YIN  Shiyong GE  Danjie LI  Ibrahim M. AHMAD  Ghulam NABI  Yanfeng SUN  Xu LUO  Dongming LI 《Integrative zoology》2023,18(5):891-905

Human commensal species take advantage of anthropogenic conditions that are less likely to be challenged by the selective pressures of natural environments. Their morphological and physiological phenotypes can therefore dissociate from habitat characteristics. Understanding how these species adjust their morphological and physiological traits across latitudinal gradients is fundamental to uncovering the eco-physiological strategies underlying coping mechanisms. Here, we studied morphological traits in breeding Eurasian tree sparrows (ETSs; Passer montanus) among low-latitude (Yunnan and Hunan) and middle-latitude (Hebei) localities in China. We then compared body mass; lengths of bill, tarsometatarsus, wing, total body, and tail feather; and baseline and capture stress-induced levels of plasma corticosterone (CORT) and the metabolites including glucose (Glu), total triglyceride (TG), free fatty acid (FFA), total protein, and uric acid (UA). None of the measured morphological parameters varied with latitude except in the Hunan population, which demonstrated longer bills than those in other populations. Stress-induced CORT levels significantly exceeded baseline levels and decreased with increasing latitude, but total integrated CORT levels did not vary with latitude. Capture stress-induced significantly increased Glu levels and decreased TG levels, independent of site. However, the Hunan population had significantly higher baseline CORT, baseline and stress-induced FFA levels, but lower UA levels, which differed from other populations. Our results suggest that rather than morphological adjustments, physiological adjustments are mainly involved in coping mechanisms for middle-latitude adaptation in ETSs. It is worth investigating whether other avian species also exhibit such dissociation from external morphological designs while depending on physiological adjustments.  相似文献   

The Research on the Construction of Concentrative Residence and Ecological Human Habitat Environment in the Rural Area of Rapidly Urbanizing Region     

LI Chang-hao  ZHU Xiao-dong  LI Yang-fan  WANG Xiang-hu  PAN Tao 《保鲜与加工》2007,(5):1-5

Under the background of the new rural construction in China, it is an important scientific question and an urgent social task to construct the harmonious society and to achieve the sustainable regional development on how to construct concentrative residence and manage the human habitat environment in the fast industrializing and typically urbanizing area. This paper analyzed the problems in the construction of the human habitat envi- ronment in the rural area of southern Jiangsu, discussed the methodologies of planning and design for rural con- centrative residence, researched the human habitat environment improvement technologies, and put forward the resolution of the recycling community for the rural inhabitation, which aimed at demonstrating the practicing in rapidly urbanizing regions.  相似文献   

Human Simulated Intelligence Motion Control for Three-link Acrobot on Horizontal Bar     

ZHANG Hu  LI Zu-shu  GU Jian-gong  CHEN Gui-qiang  TAN Zhi 《保鲜与加工》2007,(3):74-78

The design of multi-controller and multi-mode control law is presented for the three-link Acrobot on horizontal bar by employing Human simulated intelligent control method based on sensory-motor Schema.Imitated the characteristics of gymnast's swing-up and handstand movement,the control process of robot is divided by six sequence phase.Then the sensory cell and motor cell and the group of sensory-motor schema is designed based on the analysis of quality-quantity combination.The simulation results show that the controller ensures the gesture and the energy to the desired goal,farther more the motion control of giant swing backword is realized in the simulation.  相似文献   

The Biological Effects of Low Frequency Electromagnetic Fields on Cells     

LIU Xin~  ZHU Jian~  ZHONG Ji-kang~ 《保鲜与加工》2004,(1):112-115

The biological effects of ELFMES including proliferation and apoptosis of cells as well as the damage of DNA are initially evaluated with the treatment of plant cells and human pulmonary fibroblasts in low frequency magnetic fields with certain intensity and different time intervals in vitro. Cell culture, counting in vitro, fluorescent dyeing analysis and single cell gel electrophoresis are adopted. The results demonstrate that the electromagnetic field with the intensity of 3mT can affect the proliferation and apoptosis of cells significantly.  相似文献   

Slab Defect Diagnosis and Predicting System Based on Rough Set Theory     

CAO Long-han  SUN Ying-kai  CAO Chang-xiu 《保鲜与加工》2001,(1):95-98

In accordance with the special characteristics of state medium-sized construction enterprises and their reform objects of "having a free hand and enlivening", the suitable objective model, the stock cooperative system for state medium-sized construction enterprises has been put forward in this paper by analyzing the system mechanism of stock cooperative system enterprises. Then the causes for it were expounded.  相似文献   

A Study on Human Simulation Intelligent Control Algorithm to a Category of Objects     

Tu Yaqing Huang Shanglian Li Zushu 《保鲜与加工》1993,(6):8-15

Based on the model of Human Simulation Intelligent Controller (HSIC) presented briefly, a detailed algorithm was described for the servo system control by using the method of HSIC. Simulation results obtained proves the good performance of the control algorithm.  相似文献   

Nash Equilibrium Mechanic of Environmental and Human Capital     

LI Jia-ming  JING Xi-ling 《保鲜与加工》2004,(1):135-138

The externality of surrounding is introduced into the 2-capital of economical increasing models, which includes human factors, to explore the influences of circumstances contradiction on the human capital formation. The normal and special nash equilibrium of environmental and human capital is presented through a limited boundary model of overlapping generations. The character of equilibrium trail and its economical meaning are examined. The study on the model shows that laissez faire economy will be either stagnant,growing,or collapsing in the long-run,depending on the returns of the learning technology with respect to the two kinds of capital,the initial state of economy,and the relative price of the environmental and consumption goods.  相似文献