腾冲县中缅边境地区森林防火体系建设对策   总被引：1，自引：1，他引：0  

何怡 《林业调查规划》2010,35(1):84-87

腾冲县中缅边境地区森林资源极为丰富,但森林火灾隐患大,是森林防火的重点区域,森林防火工作中存在着边境不利的自然条件、火源管理难度大、防火基础设施落后等问题.为提高森林防火的综合防控能力,提出加强林火监测系统、防火基础设施、林区防火公路、林区生物防火隔离带和森林火灾扑救系统建设等森林防火体系建设对策.  相似文献   

猪伪狂犬病病毒gE抗体胶体金免疫层析检测方法的建立及应用   总被引：1，自引：0，他引：1  

廖文军  吴健敏  谭攀  覃绍敏  陈凤莲  马玲  张伟 《动物医学进展》2010,31(9)

以纯化的抗人红细胞单链抗体(ScFv)-猪伪狂犬病病毒(PRV)gE蛋白双功能融合蛋白为诊断抗原和胶体金标记物,以羊抗猪IgG包被硝酸纤维膜作为质控带,制作检测猪伪狂犬病毒gE抗体的双抗原胶体金试纸条。利用方阵滴定试验筛选出金标抗原最佳工作浓度为17.6μg,检测线诊断抗原最佳标记量为1.76μg,血清最佳稀释度为1∶10,作用时间15 min,与猪瘟病毒(CSFV)、猪细小病毒(PPV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪乙型脑炎病毒(JEV)、猪布鲁菌(Brucella)阳性血清和PRVgE缺失疫苗接种的猪免疫血清检测线均不出现红色条带,与PRV标准阳性血清反应检测线出现红色条带。试纸条操作简单,肉眼于15 min内可判定结果;试纸条在室温保存6个月,其特异性和敏感性没有明显变化;与美国IDEXX和法国LSI gE-ELISA抗体检测诊断试剂盒检测结果比较,1 164份猪血清的符合率均为90.55%。制备的胶体金试纸条具有操作简便、敏感性和特异性较高的特点,可用于PRV野毒感染的快速筛查。  相似文献   

同时检测AFB_1和ZEA免疫层析试纸条的研制     

张甄  郑丽娜  裴世春 《黑龙江八一农垦大学学报》2010,22(2):69-73,79

采用柠檬酸三钠还原法制备胶体金,将其与抗AFB1和ZEA单克隆抗体进行偶联,并把偶联物、AFB1-STI、ZEA-OVA以及硝酸纤维素膜(NC膜)等组装成免疫层析试纸条,同时进行检测条件的最优化设计。胶体金偶联AFB1和ZEA抗体最适pH同为7.0,最适抗体浓度分别为5.0和2.5μg.mL-1,NC膜上包被AFB1-STI和ZEA-OVA的浓度分别1.0和2.0mg.mL-1,为该方法可同时定性检测样品中AFB1和ZEA,其最低检测限分别达到5μg.kg-1和50μg.kg-1;与黄曲霉毒素M1、脱氧血腐镰刀菌烯醇的交叉反应率小于1%。该试纸条具有可同时检测两种真菌毒素的特点,适用于同一样品中多种毒素的快速检测。  相似文献   

Ca对AZ31镁合金CO2激光焊接接头组织和性能的影响     

严红革  陈琼  陈吉华  俞照辉  田津  石金磊 《湖南农业大学学报(自然科学版)》2010,37(9):55-59

采用添加填充材料的CO2激光焊接工艺对AZ31镁合金薄板进行焊接,研究了不同Ca含量对焊缝金属的显微组织和力学性能的影响.结果表明：当激光功率为1 500 W,焊接速度为3 m/min时, AZ31镁合金CO2激光焊接的焊缝金属中添加一定量的σCa＜0.19%元素,可以得到成分相对较均匀、组织细小的焊缝.当填充带材为AZC04(焊缝中σCa=0.19%)时,焊缝区晶粒相对细小,析出相细小弥散,此时焊接接头的力学性能最优,焊接效率基本可达到100%.  相似文献   

Phosphorus release from forest harvesting on an upland blanket peat catchment     

Michael Rodgers  Mark O’Connor  Mark Gerard Healy  Connie O’Driscoll  Zaki-ul-Zaman Asam  Mika Nieminen  Russell Poole  Markus Müller  Liwen Xiao 《Forest Ecology and Management》2010

The aim of this study was to investigate the release of phosphorus (P) to receiving waters resulting from harvesting 34-year-old lodgepole pine trees in an upland peat catchment. The study site was within a 25.3-hectare (ha) area, and was drained by a stream that received flows from ploughed furrows, mainly, via collector drains, and discharged directly to the salmonid Shrahrevagh River, Burrishoole, Co. Mayo, Ireland. The study site was divided into two parts: the upstream part was left intact and the downstream part was harvested in early Autumn 2005 following implementation of forest guidelines. Good management practices such as proper use of brash mats and harvesting only in dry weather were implemented. Two instrumented stations were established – one just upstream (US) and the other just downstream (DS) of the clearfelled area. The measurement of P concentrations at the two stations commenced in May 2005, two months before the harvesting started. The daily mean P concentration at the DS station increased from about 6 μg L⁻¹ of total reactive phosphorus (TRP) during pre-clearfelling to 429 μg L⁻¹ in August 2006. By October 2009, four years after clearfelling, the P concentrations at the DS station had returned to pre-clearfelling levels. In the first three years after harvesting, up to 5.15 kg ha⁻¹ of TRP was released from the harvested catchment to the receiving water; in the second year alone, 2.3 kg ha⁻¹ of TRP was released. Linear regression can be used to describe the relationship between TRP load and water discharge. About 80% of the total phosphorus (TP) in the study stream was soluble and more than 70% of the P release occurred in storm events, indicating that traditional buffer strips with widths of 15–20 m might not be efficient for P immobilization. The P concentrations were affected by antecedent weather conditions and highest concentrations occurred during storm events following prolonged drought periods. The water extractable phosphorus (WEP) contents in the soil were significantly higher below windrow/brash material than in brash-free areas, and whole-tree harvesting should be studied as one of the means to decrease P export from blanket peats.  相似文献   

CPA-核酸试纸条检测鸭肉源性成分方法的建立和优化     

冯涛  杨韩  张颖洁  李素芳  潘家荣 《核农学报》2016,(11):2151-2159

为建立简单、有效的动物源性成分定性检测方法,根据交叉引物恒温扩增技术(CPA)原理,以鸭属线粒体D-loop基因序列设计引物和探针,优化反应体系与恒温扩增条件,并配合核酸试纸条显色,建立鸭肉源性成分的快速检测方法。结果表明,建立的鸭D-loop基因CPA扩增体系中交叉引物、内引物、外引物分别为0.6、0.3、0.1μmol·L-1,Mg SO4为8 mmol·L-1。在63℃、60min恒温扩增条件下,单一鸭源性DNA成分检出限为0.1 ng·μL-1,对混合肉制品中鸭肉成分的检出比例为1%(相当于1ng·μL-1)。本研究建立的鸭源性成分检测方法为肉制品掺假鉴定提供了有效的手段。  相似文献   

Cryopreservation of strip spawned sperm using non‐programmable freezing technique in the blue mussel Mytilus galloprovincialis          下载免费PDF全文

Shiwen Liu  Tong Xu  Qing Liu  Xiaoxu Li 《Aquaculture Research》2016,47(12):3888-3898

In this study, a non‐programmable freezing technique has been developed with the strip spawned blue mussel (Mytilus galloprovincialis) sperm. The key parameters optimized including (1) cryoprotectant agents (CPAs); (2) cooling temperature; (3) thawing temperature; (4) sugar and amino acid supplementation and (5) sperm to egg ratio. The fertilization rate and/or integrity of plasma membrane and acrosome were used as sperm quality assessment indicators. The highest post‐thaw sperm fertilization rate of 95% was achieved, when sperm were cryopreservated in 8% dimethyl sulfoxide at 7.8 cm above the liquid nitrogen surface and thawed in a 60°C seawater bath. The addition of glucose, sucrose or trehalose in dimethyl sulfoxide did not, whereas 0.8% glycine did significantly improve the post‐thaw sperm fertilization rates. The fluorescent evaluation has demonstrated that the addition of glycine significantly improved the post‐thaw sperm acrosome integrity, revealing a positive role of glycine in the improvement of post‐thaw sperm quality in blue mussels.  相似文献   

Artist canvas as a new standard for the cotton‐strip assay     

Matthew G. Slocum  Joshua Roberts  Irving A. Mendelssohn 《植物养料与土壤学杂志》2009,172(1):71-74

  相似文献   

保护性耕作对坡耕地粮豆草等高带状种植作物生长与水土保持效果的影响     

赵旭  王汉宁  李玲玲  崔同霞 《干旱地区农业研究》2013,31(3):7-12

为有效防治坡耕地水土流失,提高坡耕地水分利用率,选用多年生作物苜蓿及小麦、鹰嘴豆、马铃薯 等一年生作物作为供试作物,设置不同带状系统研究了各种种植方式和耕作方式对作物生长、土壤水分、WUE及 水土流失的影响。结果表明,(1)各处理保持水分的能力依次为：免耕+秸秆覆盖(NTS)>免耕(NT)>传统(T);(2) 保护性耕作具有良好的防止土壤侵蚀能力,依次为：NTS>NT>T;(3)免耕+秸秆覆盖和免耕方式与传统耕作相比, 均能提高半干旱地区土壤水分和作物对水分的利用效率,减少土壤侵蚀量。从不同处理保持水土效应来看,从优 至劣的顺序为NTS_PL>T_PL>NTS_CL>T_CL>NTS_WL>T_WL。由此可见,免耕秸秆覆盖是提高水分利用效率及控制水土流失的有效措施,粮草豆隔带种植,结合保护性耕作措施不但具有蓄水保土,减免侵蚀的生态作用,而且还能提高土地利用的经济效益,宜结合生产推广利用。  相似文献   

玉米宽窄行深旋免耕精量播种机田间试验及效果   总被引：8，自引：7，他引：1  

楚杰  路海东  薛吉全  赵明 《农业工程学报》2014,30(14):34-41

为了解决目前玉米播种机械播种质量不高,良好农艺措施与农机不配套等问题,研发了一种基于宽窄行条带深旋免耕技术的玉米精量播种机。该文对该机作业后的土壤质量、土壤含水率、玉米出苗、群体光照、根系生长和产量等性状进行了分析。结果表明:采用该机播种后,土壤耕层容重显著降低(P0.05),土壤蓄水保水能力提高,玉米的播种质量明显提高,群体整齐度增加,群体透光性明显改善,具有显著的增产作用。与普通播种机械相比,该机播种后的玉米出苗率平均提高6.2个百分点,玉米株高变异系数降低8.33%,玉米根系生长量增加15.79%,玉米生育后期的群体光能截获量明显增加,产量增加11.15%。实现了优良农艺措施与农机的有效结合。  相似文献