禽腺病毒血清4型感染鸡组织中NLRP3基因转录水平的实时荧光定量PCR分析     

郭慧芳  李宁  王白玉  乔麒龙  黄庆  李永涛  王增  赵军 《畜牧兽医学报》2021,52(1):195-201

旨在分析禽腺病毒血清4型(FAdV-4)感染鸡组织中NLRP3基因的转录水平,本研究设计鸡NLRP3特异性引物,利用RT-PCR扩增NLRP3基因180 bp片段并克隆至pMD-18T载体,制备重组质粒pMD-18T-NL-RP3.以pMD-18T-NLRP3质粒作为标准品进行荧光定量PCR并建立标准曲线.通过反应条件...  相似文献   

白藜芦醇对脂多糖诱导草鱼肾脏细胞炎症的抑制作用     

陈凯  谭宏亮  习丙文  谢骏  潘良坤 《中国渔业质量与标准》2021,(1)

为评价白藜芦醇(resveratrol)对脂多糖诱导损伤草鱼(Ctenopharyngodon idella)肾脏细胞(CIK)的保护作用,本研究通过脂多糖(LPS)诱导细胞炎症损伤,测定和分析白藜芦醇对CIK细胞抗氧化因子活性和抗氧化、炎症相关基因表达变化的影响。结果表明,与对照组相比,LPS处理导致CIK细胞活力降低(P<0.05),乳酸脱氢酶(LDH)活性升高(P<0.05),降低了细胞内过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性,并导致还原型谷胱甘肽(GSH)浓度降低(P<0.05)和丙二醛(MDA)含量升高(P<0.05)。此外,LPS处理能引起CIK细胞的CAT、TNF-α、IFN-γ、IL-1基因表达显著上调(P<0.05),而对IL-10和SOD基因的转录表达无显著影响(P>0.05)。通过向培养基中添加白藜芦醇(4μg/mL)可以显著减弱LPS对CIK细胞造成的损伤,维系细胞抗氧化能力,抑制TNF-α等炎症相关基因的表达(P<0.05),促进CAT基因的表达(P<0.05)。研究认为白藜芦醇对LPS造成的CIK细胞损伤有明显的干预作用,主要原因可能是由于白藜芦醇对CIK细胞的抗氧化能力的改善以及对促炎因子表达的抑制。本研究可为白藜芦醇应用于鱼类炎症、氧化损伤相关疾病的防治提供理论依据。  相似文献   

Formation of prostaglandins, leukotrienes and paf-acether by macrophages   总被引：1，自引：0，他引：1  

R Roubin  J Benveniste 《Comparative immunology, microbiology and infectious diseases》1985,8(2):109-118

Prostaglandins (PG) and leukotrienes (LT)--arachidonic acid-dependent metabolites--and paf-acether (platelet-activating factor)--an ether phospholipid--are potent mediators of allergic and inflammatory reactions. Their structures, chemical synthesis and biosynthetic pathways have been recently described. These mediators are produced by various cells with proinflammatory activities including the macrophages upon interaction with a specific secretagogue stimulus (phagocytosis of zymosan particles, immune-complexes); in IgE-dependent hypersensitivity reactions; upon interaction with one of these mediators. Formation of these mediators by macrophages depends upon their local environment. Qualitative and/or quantitative variations in their synthesis are observed depending on the tissue they are derived from (alveole or peritoneum) and on the type of inflammation (immunologic specific or not). Their potent biological activities (increase of vascular permeability, smooth muscle contraction, cardiac and vascular effects and/or chemotactism) suggest a role for these mediators in various pathologies.  相似文献   

IN VITRO EVALUATION OF CANINE GRANULOCYTES LABELED WITH ^99mTC-HEXAMETHYLPROPYLENEAMINE OXIME:     

Russell L.  Tucker  DVM  Gregory B.  Daniel  DVM  MS  Sandra L.  Daniel  DVM  MS  PhD  Theresa  Buckman  AHT 《Veterinary radiology & ultrasound》1992,33(4):241-246

Technetium-99m hexamethylpropyleneamine oxine (^99mTc-HMPAO) and Indium-111 oxine (¹¹¹In-oxine) labeled canine gramulocytes were evaluated in vitro over a six hour period. Labeling efficiency for ^99mTC-HMPAO and ¹¹¹In-oxine labeled granulocytes was 39.6%± 8.0% and 60.6%± 17.6% (mean ± SD) respectively. The mean in vitro elution of the radiolabel ranged from 8.7-14.0% for the ^99mTc-HMPAO grannulocytes and from 6.1-9.0% for the ¹¹¹In-oxine granulocytes. Mean cell viability, for the ^99mTc-HMPAO, ¹¹¹In-oxine and non-radiolabeled control granulocytes ranged from 97.8-99.4%, 96.4-98.5% and 98.2-99.0%, respectively. The phagocytic ability of the ^99mTc-HMPAO, ¹¹¹In-oxine and control granulocytes ranged from 47.5-54.1%, 38.9-56.2% and 46.6-57.8% respectively over the six hour study period. Although labeling efficiency using ¹¹¹In-oxine was significantly (P=0.05) better than ^99mTc-HMPAO, there was no significant difference in label retention of the two radiolabels. There was no significant difference in viability or phagocytic function during the six hour study period. Considering the potential cost advantage and the superior imaging qualities of Technetium-99m relative to Indium-111, ^99mTc-HMPAO appears to be a good alternative to ¹¹¹In-oxine as a granulocyte label.  相似文献   

Susceptibility of canine, feline and human erythrocytes to oxidant-mediated injury     

Weiss DJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1988,17(3):75-78

e relative susceptibilities of feline, canine and human erythrocytes to in vitro hydrogen peroxide-induced lipid peroxidation and hemolysis were studied. At 15 minutes following exposure to hydrogen peroxide, cat erythrocytes had higher concentrations of lipid peroxidation by-products and a greater percent hemolysis as compared to dogs and humans. Erythrocytes from cats with induced sterile abscesses had lower concentrations of the antioxidant glutathione, but they did not have detectable concentrations of lipid peroxides nor were they more susceptible to in vitro lipid peroxidation or hemolysis.  相似文献   

Changes in the oxidative stress factors and inflammatory proteins following the treatment of BPH‐induced dogs with an anti‐proliferative agent called tadalafil     

Nooshin Dearakhshandeh  Asghar Mogheiseh  Saeed Nazifi  Mohammad Saeed Ahrari Khafi  Mohammad Abbaszadeh Hasiri  Kamran Golchin‐Rad 《Journal of veterinary pharmacology and therapeutics》2019,42(6):665-672

  相似文献   

β‐carotene attenuates lipopolysaccharide‐induced inflammation via inhibition of the NF‐κB,JAK2/STAT3 and JNK/p38 MAPK signaling pathways in macrophages     

Ruonan Li  Pan Hong  Xin Zheng 《Animal Science Journal》2019,90(1):140-148

β‐carotene is one of the most abundant carotenoids, has potential anti‐inflammatory effect, it has been reported that β‐carotene could suppress LPS‐induced inflammatory responses by inhibiting nuclear factor kappa B (NF‐κB) translocation, but the more detailed molecular mechanisms underlying the anti‐inflammatory action of β‐carotene remain to be fully understood. In this study, we investigated the influence of β‐carotene on the activation of JAK2/STAT3, MAPK, and NF‐κB signaling pathway induced by LPS in RAW264.7 cells and peritoneal macrophages. Cells were treated with different concentrations of β‐carotene for 3 hr after LPS treatment for 24 hr. The mRNA expression and the release of IL‐1β, IL‐6, and TNF‐α were evaluated by RT‐PCR and ELISA, and the level of signaling proteins of JAK2/STAT3, MAPK, and NF‐κB signaling pathway were detected by Western blot. The results showed that β‐carotene significantly suppressed (p < 0.05) LPS‐induced release of IL‐1β, IL‐6, and TNF‐α and their mRNA expression. LPS‐induced JAK2/STAT3, IκB/NF‐κB p65, JNK/p38 MAPK signal activation were significantly attenuated (p < 0.05) by β‐carotene in a dose‐dependent manner. In conclusion, β‐carotene could attenuate LPS‐induced inflammation via inhibition of the NF‐κB, JAK2/STAT3, and JNK/p38 MAPK signaling pathways in macrophages.  相似文献   

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota,metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica     

Chi Huan Chang  Po Yun Teng  Tzu Tai Lee  Bi Yu 《Animal Science Journal》2019,90(6):737-746

This study assessed the effect of probiotics on cecal microbiota, cecal short‐chain fatty acids (SCFAs), and the gene expression of cytokines in young specific‐pathogen‐free (SPF) chickens infected with S. enterica subsp. enterica. One‐day‐old SPF chickens (n = 105) were randomly assigned to one of the three treatment groups: control (Cont) group, Salmonella‐infected (Sal) group, and a Salmonella‐infected group treated with multi‐strain probiotics (ProSal group). All chickens except those in the Cont group were challenged orally with 1 × 10⁸ cfu/ml of Salmonella 4 days after hatching. Chickens in the Sal group exhibited more abundance of Proteobacteria than those in the Cont and ProSal groups. At the genus level, chickens in ProSal group exhibited increased numbers of Lactobacillus and Oscillospira compared with those in the other groups. Chickens in the ProSal group exhibited a significant increase of cecal SCFAs compared with chickens in the Sal group. Chickens in the ProSal group exhibited increased gene expression of anti‐inflammatory cytokines, IL‐10 and TGF‐β4, and decreased expression of the proinflammatory cytokine, IFN‐γ, in the cecal tonsil compared with those in the Sal group. The results of this study indicated that the administration of probiotics can modulate microbiota, SCFAs, and immunomodulatory activity in SPF chickens.  相似文献   

二氢槲皮素与二氢杨梅素抗炎活性对比研究     

王佳奇  刘文丛 《中国兽药杂志》2016,50(7):46-51

构建巨噬细胞验证模型,比较研究二氢槲皮素(Dihydroquercetin,taxifolin,TF)和二氢杨梅素(Dihydromyricetin,DMY)体外抗炎活性。以1 mg/L的脂多糖(LPS)诱导RAW264.7巨噬细胞建立了体外炎症模型;通过细胞毒性试验(MTT)法检测细胞存活率;Griess试剂法测定细胞上清液中NO(一氧化氮)释放量;ELISA法检测上清液中细胞因子IL-1β(白细胞介素-1β)、IL-6(白细胞介素-6)、TNF-α(肿瘤坏死因子α)和PGE2(前列腺素E2)的含量变化;通过RT-PCR法检测IL-1β、IL-6、TNF-α基因表达情况。MTT试验结果表明,二氢槲皮素和二氢杨梅素各剂量组没有表现出对巨噬细胞RAW264.7的细胞毒性。同时,二氢槲皮素高中低剂量组和二氢杨梅素高中低剂量组均能显著抑制脂多糖诱导细胞对NO、PGE2的释放(P0.01),且均表现出剂量依赖性;二氢槲皮素高中低剂量组和二氢杨梅素高中低剂量组均能有效降低IL-1β、IL-6、TNF-α基因的表达,同时能够降低脂多糖诱导细胞产生细胞因子IL-1β、IL-6、TNF-α的含量。二氢槲皮素和二氢杨梅素均具有良好的体外抗炎作用,且二氢槲皮素的抗炎作用较好。  相似文献   

Polysaccharides from Acanthopanax senticosus enhances intestinal integrity through inhibiting TLR4/NF‐κB signaling pathways in lipopolysaccharide‐challenged mice          下载免费PDF全文

Jie Han  Lixia Liu  Ning Yu  Jing Chen  Baoshan Liu  Di Yang  Guoshun Shen 《Animal Science Journal》2016,87(8):1011-1018

To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) on lipopolysaccharide (LPS)‐induced intestinal injury, mice in three treatments were administrated orally with or without ASPS (300 mg/kg body weight) for 14 days, followed by challenge with LPS or saline. At 4 h post‐injection, blood and intestinal samples of six mice / treatment were collected. The results showed ASPS ameliorated LPS‐induced intestinal morphological deterioration, proven by improved villus height (P < 0.05) and villus height : crypt depth ratio (P < 0.05). ASPS also elevated the mucosal barrier of LPS‐challenged mice, supported by reduced plasma diamine oxidase (DAO) activity (P < 0.05) and L‐lactate (P < 0.05), increased mucosal DAO activity (P < 0.05) as well as enhanced intestinal tight junction proteins expression involving occludin‐1 (P < 0.05) and zonula occludens‐1 (P < 0.05). In addition, ASPS decreased LPS‐induced secretion of inflammatory mediators, including tumor necrosis factor (TNF)‐α (P < 0.05) and prostaglandin E₂ (P < 0.05). Also, ASPS down‐regulated messenger RNA expression of toll‐like receptor 4 (TLR4) and its downstream signals, including myeloid differentiation factor 88 (P < 0.05), TNF‐α receptor‐associated factor 6 (P < 0.05), as well as nuclear factor (NF)‐κB p65 (P < 0.05) and its protein expression. These findings suggest that ASPS improves intestinal integrity under inflammation conditions connected with inhibiting TLR4/NF‐κB signaling pathways.  相似文献