Molecular and biological characterisation of Cryptosporidium in pigs   总被引：1，自引：0，他引：1  

Morgan UM  Buddle JR  Armson A  Elliot A  Thompson RC 《Australian veterinary journal》1999,77(1):44-47

OBJECTIVE: Genetic and biological characterisation of 12 isolates of Cryptosporidium from pigs and comparing them with Cryptosporidium isolates from humans and cattle. DESIGN: Cryptosporidium isolates from pigs were compared with those obtained from human and cattle using rDNA sequence analysis. The infectivity of two of the porcine isolates was determined in neonatal mice and the clinical history of the infected pigs recorded. RESULTS: Pig-derived isolates of Cryptosporidium exhibited two distinct genotypes; a porcine genotype and a bovine genotype, which is common to cattle and other livestock. The porcine genotype did not produce any infection in neonatal mice whereas the bovine genotype did. CONCLUSION: Two distinct genetically and biologically differing strains of Cryptosporidium appeared to be associated with acute diarrhoea in pigs. Whether Cryptosporidium was a primary or secondary pathogen is unclear but warrants further investigation. As the bovine genotype is known to infect humans, the results suggest that pigs can act as reservoirs of cryptosporidial infections for humans and other live-stock. The zoonotic potential of the pig-adapted genotype is uncertain and requires further study.  相似文献   

Monitoring of atrazine treatment on soil bacterial,fungal and atrazine-degrading communities by quantitative competitive PCR     

Martin-Laurent F  Piutti S  Hallet S  Wagschal I  Philippot L  Catroux G  Soulas G 《Pest management science》2003,59(3):259-268

We report the development of quantitative competitive (QC) PCR assays for quantifying the 16S, 18S ribosomal and atzC genes in nucleic acids directly extracted from soil. QC-PCR assays were standardised, calibrated and evaluated with an experimental study aiming to evaluate the impact of atrazine application on soil microflora. Comparison of QC-PCR 16S and 18S results with those of soil microbial biomass showed that, following atrazine application, the microbial biomass was not affected and that the amount of 16S rDNA gene representing 'bacteria' increased transitorily, while the amount of 18S rDNA gene representing fungi decreased in soil. In addition, comparison of atzC QC-PCR results with those of atrazine mineralisation revealed that, in response to atrazine treatment, the amount of atzC gene increased transitorily in soil pre-treated with atrazine, suggesting that accelerated atrazine biodegradation in soil could be due to a transient increase in the size of the atrazine mineralising community.  相似文献   

PET/CT FOLLOWING INTENSITY-MODULATED RADIATION THERAPY FOR PRIMARY LUNG TUMOR IN A DOG     

ELIZABETH A. BALLEGEER  LISA J. FORREST  ROBERT JERAJ  T. ROCKWELL MACKIE  R. JEROME NICKLES 《Veterinary radiology & ultrasound》2006,47(2):228-233

A primary lung tumor in a dog treated with intensity-modulated radiation therapy was imaged approximately 6 weeks and 1-year posttreatment with combined positron emission tomography (PET) and computed tomography, utilizing the radiotracers 18F-fluorodeoxyglucose and 18F-fluorothymidine. These two tracers allowed discrimination of tumor from inflammation, and demonstrated spread of tumor along airways over time after treatment. Fusion of functional imaging with anatomic imaging is a useful tool, particularly in the field of oncology, with the potential for PET markers that delineate tumor from normal or reactive tissue, and potential or actual response to therapy.  相似文献   

18S rRNA／rDNA序列分析技术在瘤胃原虫分类学研究中的应用     

钟霞  毛华明  邓卫东 《中国畜牧兽医》2007,34(12):56-58

作者指出了反刍动物瘤胃原虫分类学研究中的难点，介绍了原虫分类学中新兴的分子生物学指标(18S rRNA／rDNA序列同源性)，综述了18S rRNA／rDNA序列分析技术在瘤胃原虫分类研究中的应用。  相似文献   

广西沼泽型水牛IL-18和TNF-α基因的克隆与序列分析     

阳玉梅  郑小龙  黄维义  石云良  代华龙  张为宇 《畜牧与兽医》2007,39(12):58-60

从健康沼泽型水牛静脉无菌采血,分离外周血单核细胞(PBMCs),用刀豆素A(ConA)诱导培养3,8和12 h后,提取细胞总RNA,以Oligo(dT)18为引物合成第一链cDNA,根据牛的白细胞介素18(IL-18)和绵羊的肿瘤坏死因子α(TNF-α)基因序列设计2对特异性引物,通过PCR方法扩增出水牛IL-18和TNF-α基因,将其克隆到pMD18-T载体,测序后进行序列分析。结果试验中所克隆到的IL-18和TNF-α基因序列的开放阅读框(ORF)分别为602 bp和715 bp,与GenBank所登录的水牛IL-18和TNF-α核苷酸序列同源性为99.1%和99.7%,其推导的氨基酸序列同源性分别是99.0%和98.7%。表明成功从水牛外周血中克隆到了水牛IL-18和TNF-α基因。  相似文献   

鸡白细胞介素-6活性MTT检测方法的建立     

周全  程国富  胡薛英  李秀梅  周诗其  谷长勤 《中国兽医学报》2006,26(5):522-524

采用白细胞介素-6依赖细胞株B9建立了鸡白细胞介素-6活性的MTT检测方法。每孔培养细胞数在2.5×103~4×104范围内D值与细胞数显示有良好的线性关系,MTT的最佳保留时间为4 h,最低检测限为0.1 U/mL。应用该方法检测了健康艾维茵肉鸡25例,血清chIL-6活性为(4.33±0.75)U/mL,而25例葡萄球菌病患鸡血清chIL-6的活性为(14.05±6.87)U/mL,与健康肉鸡比较差异极显著(P<0.01),为该方法进一步临床应用奠定了基础。  相似文献   

Regulation of uterine function by cytokines in cows: Possible actions of tumor necrosis factor-α, interleukin-1α and interferon-τ     

Kiyoshi OKUDA  Ryosuke SAKUMOTO 《Animal Science Journal》2006,77(3):266-274

When animals do not become pregnant, regression of the corpus luteum (CL) is essential for normal cyclicity because it allows the development of a new ovulatory follicle. Luteal regression is caused by a pulsatile release of prostaglandin (PG) F_2α from the uterus in the late luteal phase in most mammals including cattle. Although it has been proposed in ruminants that pulsatile PGF_2α secretion is generated by a positive feedback loop between luteal and/or hypophyseal oxytocin and uterine PGF_2α, the bovine endometrium may possess other mechanisms for initiation of luteolytic PGF_2α secretion. There is increasing evidence that several cytokines mainly produced by immune cells modulate CL and uterine function in many species. Tumor necrosis factor‐α (TNF‐α) stimulates PGF_2α output from bovine endometrium not only at the follicular phase but also at the late luteal phase. Administration of TNF‐α at a high concentration prolongs luteal lifespan, whereas administration of a low concentration of TNF‐α accelerates luteal regression in cows. The data obtained from the authors’ previous in vitro and in vivo studies strongly suggest that TNF‐α is a crucial factor in regulating luteolysis in cows. The authors’ recent study has shown that interleukin‐1α mediates PG secretion from bovine endometrium as a local regulator. Furthermore, interferon‐τ (IFN‐τ) suppresses the action of TNF‐α on PGF_2α synthesis by the bovine endometrium in vitro, suggesting that IFN‐τ plays a luteoprotective role by inhibiting TNF‐α‐induced PGF_2α production in early pregnancy. The purpose of the present review is to summarize current understanding of the endocrine mechanisms that regulate uterine function by cytokines during the estrous cycle and early pregnancy in cows.  相似文献   

黑皮中熟西瓜新品种开杂18号的选育     

安水新  霍治邦  李相涛  刘宏  张存松  孟宪敏 《中国瓜菜》2006,(5):18-19

开杂18号是开封市农林科学研究所选育的黑皮、中熟西瓜新品种。全生育期103 d,果实发育期33 d,具有果个大、果皮黑且韧、品质优、易坐果、抗性强、适应范围较广等突出优点。  相似文献   

黑龙江省猫旋毛虫18S rRNA基因分子克隆及序列分析   总被引：1，自引：0，他引：1  

李冬梅  王秀荣  董小波  路义鑫  宋铭忻 《中国兽医杂志》2007,43(5):5-7

本文利用GenBank中发表的(Trichinella spiralis)18S rRNA序列为参考设计引物,对分离自黑龙江省猫体内的旋毛虫及本地毛形线虫(Trichinella nativa)的18S rRNA基因进行扩增,克隆后测序,序列分析结果表明:猫旋毛虫与旋毛形线虫基因同源性更高。  相似文献   

马白细胞介素18成熟蛋白(mEIL-18)基因在大肠埃希氏菌中的高效表达与纯化   总被引：2，自引：0，他引：2  

童铁钢  刘光亮  白宇  肖一红  王群  李少英  孟庆文  吴东来 《畜牧兽医学报》2007,38(3):307-312

用RT—PCR从经ConA刺激的马外周血单个核细胞（PBMC）中扩增出马白细胞介素18（Equine interleukin-18，EIL-18）前体蛋白（precursor EIL-18，pEIL-18）基因的cDNA，然后克隆至载体pCR2．1-TOPO中，鉴定并命名为pCR2．1-pEIL-18。自pCR2．1-pEIL-18中扩增马白细胞介素18成熟蛋白（mature EIL-18，mEIL-18）基因，并将其亚克隆至原核表达载体pET-28a（＋）中。将筛选出的阳性克隆进行测序、诱导表达并纯化其表达产物。结果表明，mEIL-18基因全长474bp，含1个开放阅读框，编码157个氨基酸的成熟蛋白；表达产物以可溶性和包涵体两种形式存在，经SDS-PAGE和Western—blot分析，重组蛋白相对分子量约为20ku，且具有免疫生物学活性。mEIL-18基因在大肠杆菌中高效表达并在非变性和变性条件下采用Ni^＋亲和层析纯化方法获得了高纯度的重组mEIL-18，为探究马白细胞介素18的生物学活性及其应用奠定了基础。  相似文献