应用盐溶蛋白电泳法对绒泡菌目黏菌的鉴定     

王晓丽  吕冬霞  李艳双  李玉 《吉林农业大学学报》2007,29(4):375-377

对煤绒菌等4种绒泡菌目黏菌显型原质团进行了盐溶蛋白的电泳分析。结果表明:煤绒菌、圈绒泡菌的特征峰分别为4个,针箍菌特征峰5个,扁绒泡菌特征峰2个。特征谱带重复性强,可以作为在原质团水平上鉴定黏菌种类的一种方法。  相似文献   

干制羊肉基因组DNA不同提取方法的比较     

陈传君  金鹭  林华  胡滨  韩国全  陈世界  张婧  安微 《核农学报》2020,34(12):2762-2768

为了研究干制加工羊肉基因组DNA的最佳提取方法,本试验采用传统酚-氯仿法、磁珠法、改良CTAB法、离心柱法分别提取干制处理后的羊肉基因组DNA,并对4种方法提取的羊肉基因组DNA浓度、纯度、完整性以及提取所需时间、PCR扩增效果等进行比较。结果表明,采用磁珠法提取DNA的效果更好,DNA浓度为118.87 ng·μL^-1,A₂₆₀/A₂₈₀值为1.89,而且此方法具有提取时间短、效率高、污染小等特点。本研究结果为干制加工羊肉基因组DNA的大批量提取和检测提供了参考依据。  相似文献   

JS018菌有机磷农药降解酶的纯化   总被引：1，自引：0，他引：1  

江玉姬  邓优锦  刘新锐  胡方平  谢宝贵  刘福阳  黎志银 《江西农业大学学报》2006,28(3)

实验对一株能高效降解几种有机磷农药菌株JS018的降解酶进行分离和纯化。经分析该降解酶主要位于细胞间质,最适盐析硫酸铵饱和度为60%~70%,粗酶液经硫酸铵沉淀、Sephadex G-100凝胶过滤和DEAE-52柱层析得到较高纯度的酶液。通过PAGE和SDS-PAGE电泳测定该酶分子量约为37KDa,N末端16个氨基酸残基测序的结果为:GAPFQNDVPPACYRFR。  相似文献   

我国尼罗罗非鱼和奥利亚罗非鱼养殖群体的遗传渐渗   总被引：19，自引：5，他引：19  

李思发 《水产学报》1995,19(2):105-111

用ＬＫＢ平板电泳仪，４．４％聚丙烯酰胺凝胶对南京罗非鱼良种场尼罗罗非鱼，奥利亚罗非鱼养殖群体肌，肝，脑，心，眼中的１０种同工酶进行电泳分析的结果表明，奥利亚罗非鱼的群体未见的多态位点，平均杂合度为０，是超“纯”的养殖群体，尼罗罗非鱼群体中有２０％的尼奥杂交鱼，其体形酷似尼罗，难以肉眼鉴别，由此可见，我国尼罗罗非鱼养殖群体中已存在遗传渐渗问题，须注意防杂和提纯。在剔除了杂交鱼后，该场尼罗罗非鱼群体的  相似文献   

金针菇航天种菌丝诱变效应的研究   总被引：2，自引：0，他引：2  

周庆红  杨寅桂  范淑英  肖旭峰 《中国食用菌》2010,29(5):37-39,62

本实验以2个金针菇航天诱变菌种(航金1号和航金2号)和地面对照菌种(江山白)为材料,进行了拮抗实验、菌丝生长速度实验、抗杂性实验和酯酶同工酶分析实验研究,结果表明,金针菇航天种与对照之间有拮抗现象,但无拮抗线。航天搭载菌株的菌丝生长速率比对照快,其中航金1号生长速度最快。航金1号和航金2号均表现出较强的抗杂性,能抑制并盖过霉菌菌丝生长。通过酯酶同工酶聚丙烯酰胺凝胶电泳分析发现,航金1号和航金2号与对照相比都出现了特异谱带,说明经过航天诱变的菌株发生了一定的突变。  相似文献   

菜粉蝶颗粒体病毒DNA限制性内切酶图谱分析     

苏金  付仓生  尤崇杓 《植物保护学报》1989,16(3):163-167

采用“酶-SDS-酚”法提取的菜粉蝶(Pieris rapae)颗粒体病毒京农801株(PrGV-JN801)DNA,A260/A280=1.85,经琼脂糖凝胶电泳呈现一条带。三种限制性内切酶消化PrGV-JN 801 DNA,其结果分别为:Hind Ⅱ,12个片段;EcoR Ⅰ,15个片段;Bam HI,12个片段。三种酶切所得限制性片段累加分子量的平均值75.04×10~6道尔顿为PrGV-JN 801DNA的分子量。  相似文献   

棉花品种抗黄萎病性与体内生化成分相关分析   总被引：12，自引：0，他引：12  

夏正俊  顾本康  吴蔼民  李经仪  陈轶林  陈小波 《植物保护学报》1994,21(4):305-310

对棉花不同抗感黄萎病性品种生化成分分析结果表明:棉花品种在无菌土中体内可溶性还原糖含量相应地高于接菌土中的含量。蕾期抗耐品种平均含量为2.276％,高于感性品种的1.417％,而铃期趋势相反。叶片单宁含量高于根部。在无菌土中,抗耐品种叶片单宁含量平均为3.58％,高于感病品种的3.28％;而在接菌条件下趋势相反。根部棉酚含量显著高于叶部,但两者极显著相关。在无菌土和接菌土中,抗性品种棉酚含量均高于耐性品种,而耐性品种又高于感性品种。棉株体内棉酚含量与棉花品种抗黄萎病性显著正相关。同工酶谱测定显示,过氧化物酶在抗感品种的无病症叶片中,酶带特征基本一致,叶片显症后表现主酶带区加宽,主酶带数增多,变化趋势与症状严重度正相关。在显症条件下,抗性品种酯酶同工酶活性略强于感性品种;与无症叶相比,显症叶的SOD酶带数由原来的4—6条减少至3—4条,抗性品种的酶带数少于感性品种1—2条。  相似文献   

Demonstration of Heterogeneous Genotypes of Taylorella equigenitalis Isolated from Horses in Six European Countries by Pulsed-Field Gel Electrophoresis     

Kagawa S  Klein F  Corboz L  Moore JE  Murayama O  Matsuda M 《Veterinary research communications》2001,25(7):565-575

Forty-six isolates of Taylorella equigenitalis were analysed by pulsed-field gel electrophoresis (PFGE) after separate digestion of the genomic DNA with ApaI and with NotI. The isolates had been obtained from horses in six European countries and were classified into 18 genotypes. In Belgium, 2 genotypes were detected in 2 isolates, in England 9 among 15, in Finland 2 in 2, in France 2 among 10, in Sweden 3 among 5, and in Switzerland 3 among 12. Two English isolates and 4 French isolates gave identical PFGE profiles to those of Kentucky 188 from the United States. A common genotype was found in 5 isolates from Belgium and England and also in 10 isolates from France and Switzerland. The analysis of genomic DNA from 12 isolates of T. equigenitalis obtained from male horses in France, Sweden and Switzerland gave no evidence of a sex-related difference in the genomic DNA. Genomic DNA from 11 streptomycin (STM)-susceptible isolates obtained in Sweden and Switzerland were classified into four genotypes by PFGE. Each of the six genotypes determined among the 17 isolates from these two countries had single phenotypes for resistance or susceptibility to STM.  相似文献   

Application of a putative fatty-acid binding protein to discriminate serologically the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from other Meloidogyne species     

Christophe Tastet  Florence Val  Michel Lesage  Lionel Renault  Laurent Marché  Michel Bossis  Didier Mugniéry 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(8):821-832

Two major proteins, Mcf-A67 and Mcf-B66, were identified by mini two-dimensional polyacrylamide gel electrophoresis in order to distinguish the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from eight other species. These quarantine proteinic markers have been microsequenced after enzymatic digestion. The internal amino acid sequences exhibit similarities to members of a family of low molecular weight intracellular lipid-binding proteins. Moreover, to explore a simple, rapid, and inexpensive way to identify the two quarantine nematodes, dot blot hybridizations were performed using an antiserum (A67) produced from the longest amino-acid sequence of the protein Mcf-A67. Although several proteins stained on the M. chitwoodi and M. fallax western blot membranes, the two nematodes were easily distinguished from other root-knot nematodes, on dot blot assays with soluble proteins extracted from a single female. Because of its specificity and sensitivity, the use of the A67 antiserum to improve the diagnosis of the two European quarantine root-knot nematodes is discussed.  相似文献   

大麦云纹斑病菌对杀菌剂的抗性检测及同工酶谱类型   总被引：2，自引：0，他引：2  

刘波  蔡德华  苗容  S. J. Kendall  D. W. Hollomon 《植物保护学报》1996,23(3):247-252

1993～1994年大麦云纹斑病菌(Rhynchosporium secalis)对杀菌剂多菌灵、三唑醇的抗药性监测表明,该菌对两种杀菌剂的敏感程度发生了变化,并且首次在田间发现了大麦云纹斑病菌的多抗菌株。这类菌株对多菌灵、乙霉威及三唑醇都具有抗药性,且抗性程度很高。与1990～1991年得到的资料相比,随着杀菌剂选择压力的增加,大田群体中三唑醇高抗菌株比例逐年增加。对该菌同工酶分析结果表明,α—酯酶有7种表现型,磷酸葡萄糖变位酶有2种表现型,过氧化氢酶有3种表现型,无论对去甲基抑制剂类(DMI)表现抗性或敏感菌株,无论调节酶还是非调节酶,其酶谱表现型的变异基本相似。这表明大麦云纹斑病菌对DMI类抗药性突变存在于多个菌系的基因型背景群体中,而不是只起源于单一的广泛分布的菌系。  相似文献