Application of a putative fatty-acid binding protein to discriminate serologically the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from other Meloidogyne species     

Christophe Tastet  Florence Val  Michel Lesage  Lionel Renault  Laurent Marché  Michel Bossis  Didier Mugniéry 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(8):821-832

Two major proteins, Mcf-A67 and Mcf-B66, were identified by mini two-dimensional polyacrylamide gel electrophoresis in order to distinguish the two European quarantine root-knot nematodes, Meloidogyne chitwoodi and M. fallax, from eight other species. These quarantine proteinic markers have been microsequenced after enzymatic digestion. The internal amino acid sequences exhibit similarities to members of a family of low molecular weight intracellular lipid-binding proteins. Moreover, to explore a simple, rapid, and inexpensive way to identify the two quarantine nematodes, dot blot hybridizations were performed using an antiserum (A67) produced from the longest amino-acid sequence of the protein Mcf-A67. Although several proteins stained on the M. chitwoodi and M. fallax western blot membranes, the two nematodes were easily distinguished from other root-knot nematodes, on dot blot assays with soluble proteins extracted from a single female. Because of its specificity and sensitivity, the use of the A67 antiserum to improve the diagnosis of the two European quarantine root-knot nematodes is discussed.  相似文献   

大麦云纹斑病菌对杀菌剂的抗性检测及同工酶谱类型   总被引：2，自引：0，他引：2  

刘波  蔡德华  苗容  S. J. Kendall  D. W. Hollomon 《植物保护学报》1996,23(3):247-252

1993～1994年大麦云纹斑病菌(Rhynchosporium secalis)对杀菌剂多菌灵、三唑醇的抗药性监测表明,该菌对两种杀菌剂的敏感程度发生了变化,并且首次在田间发现了大麦云纹斑病菌的多抗菌株。这类菌株对多菌灵、乙霉威及三唑醇都具有抗药性,且抗性程度很高。与1990～1991年得到的资料相比,随着杀菌剂选择压力的增加,大田群体中三唑醇高抗菌株比例逐年增加。对该菌同工酶分析结果表明,α—酯酶有7种表现型,磷酸葡萄糖变位酶有2种表现型,过氧化氢酶有3种表现型,无论对去甲基抑制剂类(DMI)表现抗性或敏感菌株,无论调节酶还是非调节酶,其酶谱表现型的变异基本相似。这表明大麦云纹斑病菌对DMI类抗药性突变存在于多个菌系的基因型背景群体中,而不是只起源于单一的广泛分布的菌系。  相似文献   

马铃薯纺锤块茎类病毒的检测和防治   总被引：5，自引：1，他引：5  

崔荣昌  李芝芳  李晓龙  王国学 《植物保护学报》1992,19(3):263-269

马铃薯纺锤块茎类病毒病(potato spindle tuber viroid,PSTVd)是一种严重为害马铃薯生产的病害,降低产量20%—30%。防治的主要措施是应用无类病毒的种薯。由于目前还没有脱掉类病毒的有效措施,只能从未被饱和侵染的群体中鉴定筛选出未被侵染的个体,再脱掉其它病毒,作为核心繁殖材料。1987年以来,利用自制的电泳设备,以往复聚丙烯酰胺凝胶电泳法(return-polyacrylamide gel electrophoresis,R-PAGE)检测类病毒,筛选出未感病的个体,再用茎尖组织培养法脱掉其它病毒。经用马铃薯卷叶病毒等8种病毒酶标抗体鉴定筛选,获得既无类病毒也无主要马铃薯病毒的克新1、2、3和4号等主栽马铃薯品种的核心种。并已提供给省内外的良种场繁殖推广。1989和1990年抽样检测克山良种场繁殖的原种、一级和二级良种,未检测到类病毒。  相似文献   

Specificity of polycllonal and monoclonal antibodies for the identification of Xanthomonas campestris pv. campestris     

A. A. J. M. Franken  J. F. Zilverentant  P. M. Boonekamp  A. Schots 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):81-94

Polyclonal and monoclonal antibodies (PCAs and MCAs), produced to whole cells and flagellar extracts ofXanthomonas campestris pv.campestris (Xcc), respectively, were tested for specificity. In immunofluorescence microscopy (IF) the three PCAs tested, reacted at low dilutions with all Xcc strains, some other xanthomonads and non-xanthomonads. At higher dilutions most cross-reactivity with non-xanthomonad strains disappeared. However, the cross-reactivity with strains ofX. c. pv.vesicatoria (Xcv),X. c. pv.amoraciae (Xca) andX. c. pv.phaseoli var. fuscans (Xcpf) remained.Six MCA-producing cell clones viz. 20H6, 2F4, 18G12, 10C5, 17C12 and 16B5 were selected for specificity tests with an enzyme immunoassay (EIA), IF and a dot-blot immunoassay (DBI). None of the MCAs reacted with all Xcc strains in IF and EIA. In DBI, only MCAs 17C12 and 16B5 reacted with all Xcc strains. All six MCAs tested, cross-reacted in one of either tests with other pathovars ofX. campestris, such as Xcv or Xca. The MCAs were also tested in immunoblotting experiments using total bacterial extracts, cell envelope and flagellar extracts. MCAs 20H6, 2F4, 18G12 and 10C5 reacted with the lipopolysaccharide (LPS) of Xcc. MCAs 16B5 and 17C12 reacted with a 39 kilodalton and a 29 kilodalton protein, respectively.It is concluded that the PCAs and MCAs discussed in this study may be used for routine identification and differentiation of (a group of) Xcc strains. The significance of the cross-reactions with other pathovars ofX. campestris needs to be determined by testing seed lots.  相似文献   

条锈菌侵染早期小麦叶片RNA和rRNA的合成   总被引：1，自引：0，他引：1  

商鸿生  张慧 《植物病理学报》1995,25(3):215-220

 采用示踪方法研究了条锈菌侵染早期小麦叶片的RNA和rRNA合成。结果表21h期间,表现不亲和性反应的叶片总RNA合成有所增加,而亲和性反应寄主叶片则低rRNA合成未受侵染影响。在接种后39~45h期间,只有呈亲和性反应的寄主叶片RNA和大幅度增加。对rRNA各组分合成的测定表明条锈菌侵染所影响的只是大分子量rRNA,胞质和叶绿体rRNA的合成也存在差别。文中讨论了上述变化的病理学意义。  相似文献   

The induction of 1,3-β-glucanases and other enzymes in groundnut leaves infected with Cercospora arachidicola     

S. Roulin  A. J. Buchala 《Physiological and Molecular Plant Pathology》1995,46(6)

Infection of groundnut leaves with the early leaf spot pathogen Cercospora arachidicola leads to a marked increase in extracellular 1,3-β-glucanase activity, limited to the infected tissue. Three isoforms of low molecular weight and extreme pI values, typical of pathogenesis-related proteins, were induced. These β-glucanases, when acting together, were capable of degrading the pathogen cell wall in vitro. Glucanases from homogenates of infected leaf tissue were partially purified by ion-exchange chromatography to give enzymes with molecular weights of 35, 32 and 20 kDa and pI values of 3·8, 3·6 and > 9, respectively. They were electrophoretically identical to the β-glucanases found in the intercellular washing fluid. Treatment of groundnut plants with 200 μM mercuric chloride induced the accumulation of identical extracellular β-glucanases. During the course of the infection an increase in peroxidase activity was also observed, but chitinase activity remained more or less constant.  相似文献   

樟子松松针锈病病原菌——春孢子形态及酯酶同工酶变异     

薛煜  程东升  韩晓芸  孙立夫  时春玲 《东北林业大学学报》1996,(6)

樟子松松针锈病病原菌——鞘锈菌（Ｃｏｌｅｏｓｐｏｒｉｕｍ）的分类历来很乱。传统的分类方法都是依冬孢子形态和冬孢子寄主来进行的。但近年的研究表明１种鞘锈菌的春孢子可以侵染２种或２种以上的冬孢子寄主，所以按冬孢子寄主来划分鞘锈菌的种是不合理的。文中在病害症状观察和春孢子表面形态观察的基础上，首次对樟子松３种不同形态的春孢子的酯酶同工酶进行了分析，从而为鞘锈菌的分子分类学研究奠定了基础。  相似文献   

猴丰1号新菌株的选育及其酯酶同工酶分析   总被引：2，自引：0，他引：2  

丁凤珍  金建康 《食用菌学报》1996,3(2):5-8

采用子实体组织分离与系统筛选法,从福建猴头的变异株中选育出了定名为“猴丰1号”的新菌株。该菌株菌丝体生长势强,发菌迅速;子实休菇形大,产量高。鲜菇产量比出发株福建猴头增加69.3％,比著名的常山99菌株增加16.8％。酯酶同工酶分析显示:猴丰1号的酯酶谱型与福建猴头相似,但有1条酶带发生了变异。由此说明,它确是源于福建猴头的自然变异株。  相似文献   

Milk Protein Polymorphism in Kangayam Cattle     

Jeichitra V  Kandasamy N  Panneerselvam S 《Tropical animal health and production》2003,35(2):147-153

This paper reports the milk protein polymorphism, the allele frequencies of variants and the possible linkages among various combinations of milk protein phenotypes in the Kangayam cattle of south India. Milk samples from 156 Kangayam cows were typed by starch gel and polyacrylamide gel electrophoresis for caseins and whey proteins, respectively. All the four milk protein components studied, _s1-casein, -casein, -lactoglobulin and -lactalbumin, exhibited polymorphism with high allele frequencies of 0.9231±0.0151 for _s1-casein C, 0.9263±0.0148 for -casein A, 0.9135±0.0159 for -lactoglobulin B and a relatively high frequency of 0.6218±0.0275 for -lactalbumin A. The mean heterozygosity estimated over all the four milk protein loci was 0.2420. Genetic equilibrium was observed among all the loci studied, except -lactalbumin. Linkage analysis confirmed the non-independence between _s1- and -caseins and between caseins and -lactalbumin phenotypes.  相似文献   

狗牙根品种及辐射诱变M1代变异植株的过氧化物同工酶分析   总被引：2，自引：0，他引：2  

王文恩  包满珠  张俊卫 《中国草地学报》2007,29(1):71-76

采用聚丙烯酰胺凝胶电泳法对辐射诱变M1代变异植株及其对照、‘Tifway’和‘Tifgreen’共18个狗牙根材料的过氧化物同工酶进行了分析。结果表明：供试材料共出现了97条酶带，单个材料最多为8条，最少只有2条；材料HN009和HN010、HN012和HN013的POD酶带特征完全相同；HN015和HN016的P013酶带在P8位点上有强弱不同，HN004和HN018的POD酶带在P5和P6位点上有强弱不同，它们的酶带数和位点均相同；其它各材料之间酶带总数不同，在各个区域分布的位点及酶谱特征明显不同。除HN010和其对照HN009的酶谱特征相同外，其它形态变异植株和其对照之间的酶谱特征均存在明显差异。  相似文献