血清可溶性细胞间粘附分子在大肠癌患者组织中的检测及其意义     

黄汉周 《湛江医学院学报》2005,23(3):255-256

目的:探讨血清可溶性细胞间粘附分子1(sICAM-1)的检测在大肠癌患者中的临床意义。方法:采用双抗体夹心ELISA法检测50例大肠癌、30例大肠良性病变及30例健康体检者血清sICAM-1水平,并比较大肠癌患者术前、术后,转移、无转移sICAM-1水平变化。结果:大肠癌患者血清sICAM-1水平明显高于良性病变患者及正常人(P<0.01);大肠癌患者术后血清sICAM-1水平较术前明显下降(P<0.01);有转移的大肠癌患者血清sICAM-1水平高于无转移的患者(P<0.01)。结论:血清sICAM-1水平与大肠癌的发生、发展密切相关,可作为大肠癌新的检测指标之一。  相似文献   

Obituary-Roger Brereton Marshall     

Bill Manktelow 《New Zealand veterinary journal》2013,61(6):252-253

Roger Marshall was born in Christchurch on 27 May 1933 and died at his home in Palmerston North on 24 October 2001. He graduated BVSc from the University of Sydney in 1958 and began his professional career in clinical veterinary practice in Morrinsville. Wishing to specialise in veterinary microbiology, he later enrolled in the postgraduate course for the Diploma in Microbiology at the University of Otago under Professor JAR Miles. On gaining the Diploma in 1963, Roger was appointed a foundation lecturer in veterinary microbiology in the newly formed Faculty of Veterinary Science at Massey University.  相似文献   

Integrin Beta1 Mediates Hepatocellular Carcinoma Cells Adhesion to Type IV Collagen Coated Surfaces     

《保鲜与加工》2003,(9):82-84

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草鱼源致病性维氏气单胞菌的鉴定及其黏附特性分析     

李槿年  马彤彤  房慧  韩雨希  刘心媛 《安徽农业大学学报》2019,46(4):603-609

采用表型鉴定与细菌16S rRNA基因序列分析对从患病草鱼体内分离的病原菌株16-1进行分类鉴定,综合该菌株的表型特征与16S rRNA基因序列,确定其为维氏气单胞菌。随后,对该菌株的细胞黏附特性及其携带的黏附素基因进行检测与分析。结果显示,分离菌株能以聚集性方式黏附于鲤鱼上皮瘤细胞（EPC）周围,平均黏附菌数随共孵育时间延长而增加,90 min达到峰值,说明该分离株具有良好的细胞黏附性。黏附因子检测表明,该菌株同时携带ompAI、ompAII和ahl13种黏附素基因,这些黏附素基因在分离菌株与不同来源参考株之间的核苷酸序列和氨基酸序列同源性分别介于95.8%～99.5%和95.0%～100%（ompAI）,96.3%～99.1%和97.9%～100.0%（ompAII）,78.6%～99.6%和75.5%～99.4% （aha1）,说明所携带的ompAI和ompAII黏附素基因在不同来源的维氏气单胞菌之间具有较高的保守性。  相似文献   

水性聚氨酯漆料干燥速率及漆膜性能影响规律研究     

张家祖  徐康  袁少飞  王洪艳  张建  张文标  李琴 《中南林业科技大学学报(自然科学版)》2019,(1):134-140

以4种水性聚氨酯漆料为研究对象,系统研究了漆料和基材类型、涂饰工艺对水性漆表干、实干时间和漆膜硬度、附着力及耐磨性的影响规律。结果表明:漆料的表实干时间及漆膜性能很大程度受成膜树脂本身性能影响;基材影响漆料表实干时间、漆膜硬度和附着力,对漆膜耐磨性影响不大,漆料涂饰在樱桃木和水曲柳上的干燥速率最快,在樱桃木、沙比利和水曲柳上的硬度最佳,达2 H,在樱桃木、沙比利和红橡上的附着力最佳,达0级;涂饰工艺对漆料的表干、实干时间以及漆膜硬度、附着力、耐磨性的影响都不明显,3种工艺涂饰后漆膜硬度和附着力分别为2 H和0级,漆膜耐磨性差别不大。  相似文献   

基于活动苗盘的钵苗脱盘基质损失的试验研究     

冯世杰  颜波  全伟  吴明亮 《湖南农业大学学报(自然科学版)》2019,45(6)

针对穴盘苗脱盘过程中基质损失导致取苗成功率降低的问题,以活动苗盘所育油菜钵苗为研究对象,选取苗盘锥角角度(7°、9°、11°)、基质压实度(1.0、1.1、1.2)、基质含水率(55%、60%、66%)为试验因素,以苗钵基质损失率为试验指标值,进行正交试验,研究苗钵脱盘过程中各因素对苗钵基质损失率的影响。结果表明:穴盘苗脱盘过程中,苗钵基质平均损失率为3.09%;苗盘锥角角度和基质压实度对基质损失率的影响极显著,基质含水率对基质损失率的影响显著;各因素对苗钵基质损失率影响大小依次为基质压实度、苗盘锥角角度、基质含水率;当苗盘锥角角度为11°、基质压实度为1.2、基质含水率为66%时,苗钵基质损失率最小,为2.62%。  相似文献   

野猪粪便中乳酸菌的分离鉴定及特性研究     

王翌  李淼  李永锋  孙元  仇华吉 《中国农业科学》2020,53(14):2964-2973

【目的】筛选安全的、具有优良特性的乳酸菌菌株,进一步研发益生制剂,为饲料添加剂等动物相关产品提供资源。【方法】从我国黑龙江省大兴安岭地区采集野猪粪便样品13份,编号后置于4℃保温箱迅速运回实验室,利用MRS培养基分离纯化乳酸菌。使用细菌基因组DNA提取试剂盒提取分离菌株的基因组DNA,利用细菌16S rDNA通用引物进行PCR鉴定,将扩增得到的序列测序后在NCBI上使用BLAST与GenBank数据库中序列进行对比分析,确定各菌株的分类学地位。将鉴定后的乳酸菌菌株分别接种于酸性（pH 3.0）和含胆盐（0.3%）的MRS培养基,在不同条件下评价乳酸菌菌株的耐酸、耐胆盐特性。将过夜培养的乳酸菌于室温条件下静置,在不同时间测定其OD_600nm,进行自凝集能力评价;过夜培养的菌株分别与致病性埃希氏大肠杆菌、金黄色葡萄球菌和鼠伤寒沙门氏菌3种致病菌混合后于室温静置,进行共凝集能力检测。在体外,分别进行乳酸菌菌株对Caco-2细胞和IPEC-J2细胞的黏附能力测定,评价不同菌株的黏附能力。通过测定乳酸菌菌株对致病性埃希氏大肠杆菌、金黄色葡萄球菌和鼠伤寒沙门氏菌3种致病菌的抑菌环直径,评价分离菌株的抑菌活性。通过体内外试验评价乳酸菌菌株的安全性。在体外,分别以模式菌株嗜酸乳杆菌和金黄色葡萄球菌作为阴性对照和阳性对照,将3株乳酸菌菌株和对照菌株在血平板上划线,37℃厌氧孵育18—24 h,观察细菌菌落周围是否形成溶菌环,评价分离菌株的溶血特性。使用文献中已报道的毒力因子引物对分离的乳酸菌菌株进行PCR扩增,检测是否存在毒力因子的编码基因,评估分离菌株的安全性。在体内,将过夜培养的乳酸菌连续饲喂7周龄的BALB/c小鼠21 d,分别测定小鼠的初始体重和最终体重,观察计算体重变化情况;饲喂21 d后,解剖获取小鼠的脾脏、肝脏和肾脏计算器官指数,评价分离乳酸菌菌株的体内安全性。【结果】从野猪粪便中分离得到3株对酸和胆盐具备一定耐受力的乳酸菌,经鉴定分别为蒙氏肠球菌（Enterococcus mundtii）、耐久肠球菌（Enterococcus durans）和黏膜乳杆菌（Lactobacillus mucosae）。3株乳酸菌菌株均表现出较强的自凝集能力和对致病菌的共凝集能力,同时对Caco-2细胞和IPEC-J2细胞均表现出较强的黏附能力,抑菌试验结果显示黏膜乳杆菌对3种致病菌均具备较强的抑菌活性。经体内外安全性评价,3株乳酸菌菌株无溶血性,且均未检测到毒力基因,经其连续饲喂的小鼠行为表现正常、状态良好,其中,与对照组相比,黏膜乳杆菌饲喂后小鼠增重显著。【结论】从大兴安岭野猪粪便中分离的3株乳酸菌（特别是黏膜乳杆菌）具有良好的特性和安全性,具备进一步开发益生菌制剂的潜力。  相似文献   

Influence of taurine on adhesion molecule expression in acute lung injury following limb ischemia-reperfusion in rats     

MEN Xiu-li  XU Ya-ping  LI Hong-jie  ZHANG Lian-yuan  DONG Shu-yun 《园艺学报》2008,24(2):396-398

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Hull to caryopsis adhesion and grain skinning in malting barley: Identification of key growth stages in the adhesion process     

《Journal of Cereal Science》2016

Strong adhesion between the hull and the caryopsis is essential for barley to be of good malting quality. Poor hull adhesion, a condition known as grain skinning, is undesirable for malting and downstream processes. At present, the processes mediating hull adhesion during grain development are poorly understood. The barley cultivar Chariot was grown in greenhouse conditions and grain development was recorded at defined growth stages to examine the timing of hull adhesion. Initiation of adhesion was first observed when caryopsis fresh weight and volume were approaching their maximum at 19 days after anthesis, during early dough. Hull adhesion was complete by 27 days after anthesis, or soft dough. Sections of developing grains were observed using light and transmission electron microscopy to examine a lipid-rich cementing layer believed to be responsible for adhesion between the hull and the pericarp. Evidence for a lipid-rich cementing material was supported by the observation that neither pectinase nor cellulase effected hull loosening. Grain growth, the presence of globular material originating from the pericarp and an electron dense material in the cementing layer are discussed in relation to hull adhesion. Grain skinning could be caused by poor adherence of cuticular material or inadequate fusion between cuticles.  相似文献   

Role of sphingosine 1-phosphate on high glucose-induced vascular endothelial cell dysfunction     

LIU Wei-hua  LIN Shuang-feng  SHI Ji-xiang  PAN Ting  CHEN Qiu-mei  WANG Shuo-ting  SHANG Hui 《园艺学报》2016,32(2):245-250

AIM: To explore the role of sphingosine 1-phosphate (S1P) in the dysfunction of vascular endothelial cells exposed to high glucose. METHODS: In human aortic endothelial cells cultured under high-glucose (22 mmol/L glucose) medium, nitric oxide (NO) level, polymorphonuclear neutrophil-endothelial cell adhesion rate, protein level of intercellular adhesion molecule-1 (ICAM-1), migration of endothelial cells and Akt/endothelial nitric oxide synthase (eNOS) pathway activation were observed after S1P, sphingosine kinase-1 inhibitor and/or Akt inhibitor treatments. RESULTS: S1P decreased NO level, increased polymorphonuclear neutrophil adhesive rate, enhanced ICAM-1 protein level, and inhibited migration of endothelial cells and activation of Akt/eNOS pathway in endothelial cells cultured under high-glucose condition. Sphingosine kinase-1 inhibitor, which reduced S1P content, significantly improved the above endothelial cell function indexes and restored the activation of Akt/eNOS pathway. CONCLUSION: S1P promoted high glucose-induced dysfunction of endothelial cells probably by inhibiting the activation of Akt/eNOS signal pathway. Targeting S1P is expected to become one of potential treatment strategies to reduce endothelial cell dysfunction.  相似文献