转染技术在顶复门原虫研究中的应用     

闫文朝  王天奇  索勋  赵战勤  韩利方 《中国农业科学》2009,42(10):3693-3699

 自1993年转染技术首次在弓形虫中成功报道以来,顶复门原虫的转染研究得到了快速发展,不仅在弓形虫、疟原虫和住肉孢子虫等实现了稳定转染,而且在体外细胞上生长效率较低的柔嫩艾美耳球虫也相继实现了瞬时转染和稳定转染。顶复门原虫转染技术的日渐成熟及其在顶复门原虫研究中的应用,为顶复门原虫基因功能和免疫学等领域研究提供了新思路和新的有力工具。本文结合本课题组近几年来的研究成果,综述了转染技术及其在顶复门原虫基因表达调控、基因功能、耐药性分子机理、药物敏感性试验和免疫学等研究中应用的最新进展。  相似文献   

10kV配网工程三项带电作业工序的探讨     

黄伟鹏 《湖南农机》2012,(5):236-237

为了减少因故障停电影响客户用电,近几年在10kV配网施工中大力开展了带电作业。文从10KV施工及施工管理工作经验出发,对10KV带电施工作业的几项常见施工作业操作步骤进行整理和总结。  相似文献   

Population growth and productivity of the cyclopoid copepods Paracyclopina nana,Apocyclops royi and the harpacticoid copepod Tigriopus japonicus in mono and polyculture conditions: a laboratory study     

Kyun‐Woo Lee  Hans‐U. Dahms  Heum Gi Park  Jung‐Hoon Kang 《Aquaculture Research》2013,44(5):836-840

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大连地区玉米螟越冬期间气象条件越冬后活虫数的影响   总被引：1，自引：1，他引：0  

尹文昱尹远渊  徐春红 《中国农学通报》2012,28(15):185-188

为研究大连地区玉米螟越冬期间气象条件对越冬后活虫数的影响,以期为防治玉米螟提供科学依据。利用大连地区最近10年气象资料和玉米螟越冬后活虫数资料,对玉米螟越冬后活虫数与越冬期间气象要素进行相关性分析。结果表明,玉米螟冬后百杆活虫数受上年10月下旬-11月份温湿条件影响较大。此间温度低有利于其越冬成活;11月份降水多,冬后百杆活虫数多。建立大连地区玉米螟冬后百杆活虫数统计预报模式。  相似文献   

Current status of the giant freshwater prawn ( Macrobrachium rosenbergii) industry in China,with special reference to live transportation     

Guoliang Yang  Mike Frinsko  Xuefeng Chen  Junyi Wang  Guo Hu  Qiang Gao 《Aquaculture Research》2012,43(7):1049-1055

The giant freshwater prawn Macrobrachium rosenbergii was first introduced into mainland China by the Chinese Academy of Agricultural Sciences from Japan in 1976. It was cultured at the Guangdong Province Fishery Institute (now as Pearl River Fishery Institute, Chinese Academy of Fishery Sciences). Following these early efforts, giant freshwater prawn culture has gradually spread to northern and inland areas. At this time (2011), China has become the largest producer of giant freshwater prawn in the world. Due to a preference of cooking with fresh aquatic products, Chinese retail markets have developed which have special live‐holding counters. From small rural shops to urban supermarkets and specialty restaurants, live seafoods are commonly available whereby customers pick out the products they wish to purchase and consume. Giant freshwater prawns are produced in over 35 countries throughout the world. They are usually sold either fresh, on‐ice, or frozen. What makes the Chinese prawn industry unique is its focus on a live transportation and marketing model. As a result, numerous innovative live‐transport technologies have been developed which have been key to the success of this expanding domestic industry. In this article, we would like to give a general overview of the giant freshwater prawn industry, its status in China, and the special role live‐transportation has for expanding production of giant freshwater prawns.  相似文献   

Notes on Propagation of Butterfly Peacock Bass Cichla ocellaris (Bloch & Schneider, 1801) in a Tropical Fish Hatchery          下载免费PDF全文

J Wesley Neal  Karina M Olivieri‐Velázquez 《Aquaculture Research》2015,46(7):1785-1787

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A first step towards improving copepod cultivation using modelling: the effects of density,crowding, cannibalism,tank design and strain selection on copepod egg production yields          下载免费PDF全文

Guillaume Drillet  Fabien Lombard 《Aquaculture Research》2015,46(7):1638-1647

Copepods are the optimal live feed for hatcheries and improvement of cultivation techniques, to provide a constant food source, is crucial for the expansion of the industry. However, studies based on experimental work and real observations can be labour intensive and expensive. A simple model was developed based on the well‐known life history traits of Acartia tonsa to describe batch cultures and their productivity. Model results were compared to observations from real cultures. For maximizing egg production yields, the optimal stocking density of copepods should be adapted to the design (depth) of the culture tanks. At high densities, stress due to encountering conspecifics, as well as cannibalism of eggs by adults, limits egg production yields. Using this model, the potential selection efficacy of copepod strains was also evaluated in order to increase production yields. Selecting larger copepods increases the egg production per litre of culture, but decreases the optimal stocking density and the range of densities at which egg production yield is high, and vice‐versa. Selecting copepods that are less affected by stress due to conspecifics only affect production yields at very high adult densities. However, selecting copepods with a high Specific Growth Rate (SGR), or improving their SGR, was found to be an alternative which did not affect the optimal cultivation densities but improved egg production yields.  相似文献   

不同伪狂犬病活疫苗免疫效力比较   总被引：1，自引：0，他引：1  

陈娟 《中国兽药杂志》2017,51(1):15-18

为临床科学选用疫苗,对来自三个生物制品厂的伪狂犬病活疫苗的免疫效力进行了评价。试验选取35日龄伪狂犬阴性猪20头,试验根据疫苗接种情况分为5组,分别为:免疫疫苗稀释液的对照组(第一组)、免疫进口活疫苗组(第二组)、免疫国产活疫苗组(第三组)、免疫本公司ST细胞系增殖制备活疫苗组(第四组)、免疫本公司鸡胚成纤维细胞增殖制备活疫苗组(第五组),然后进行免疫监测和效力评价,观察不同疫苗组仔猪的抗体水平后攻毒保护情况。结果显示:一次免疫后各组抗伪狂犬病毒抗体水平增长缓慢,进行二次免疫后,抗体水平迅速增加并保持较高水平。攻毒后,从身体耐受性、体温变化、体重下降率以及g E抗体水平来看,第二组疫苗B和第四组疫苗D耐受性较强,较快恢复健康,体重下降率较小且g E抗体水平较高。  相似文献   

猪瘟脾淋毒耐热保护剂活疫苗内毒素检测方法的建立与应用     

郭广君  吕素芳  苗立中  毕妍丽  沈志强 《中国动物检疫》2010,27(2):35-37

依据《中华人民共和国兽药典》2005年版(一部)附录112-115和《中华人民共和国药典》2005年版(三部)ⅫE收载的细菌内毒素检测方法及指导原则进行实验。研究猪瘟脾淋毒(耐热保护剂)活疫苗对细菌内毒素检查试验的干扰情况,并建立其内毒素检测方法。结果猪瘟耐热保护剂活疫苗在10倍稀释后对灵敏度为0.25EU/ml的鲎试剂无干扰,细菌内毒素检测方法可用于猪瘟耐热保护剂活疫苗内毒素的检测。  相似文献   

生猪肉产量预测的非接触实时在线机器视觉系统   总被引：2，自引：2，他引：0  

田芳  彭彦昆 《农业工程学报》2016,32(2):230-235

为了准确估测生猪肉产量,该文提出了一种自动提取生猪活体特征的方法,建立了生猪肉产量预测算法。首先,基于自主研发的生猪肉产量预测的机器视觉系统装置实时采集54头活体大白猪的俯视图和侧视图,然后根据图像处理技术对所得的图像进行分析,提取生猪的体长、胸宽、臀宽、体高等体尺参数值;同时通过称量装置获得每头猪的活体质量。其次,将54头生猪样品按2∶1的比例分为校正集和验证集,基于体长、胸宽、臀宽、体高和体质量5个特征参数分别利用多元线性回归法(multiple linear regression,MLR)和偏最小二乘回归法(partial least-squares regression,PLSR)建立不同的肉产量预测模型,通过比较各预测模型的估测精度、相关系数等,确定最佳预测模型。分析结果表明,5个参数中体质量对肉产量预测的权重最大;偏最小二乘回归方法的预测结果较好,最佳预测模型的预测相关系数为0.95,预测误差为3.09 kg。试验结果证实,在检测系统中使用基于生猪的体尺参数和体质量建立的偏最小二乘回归预测模型可快速准确预测生猪肉产量,适于实际生产应用。  相似文献