Growth,feed utilization and liver histology of juvenile common sole (Solea solea L.) fed isoenergetic diets with increasing protein levels     

Pier Paolo Gatta  Luca Parma  Ilaria Guarniero  Luciana Mandrioli  Rubina Sirri  Ramon Fontanillas  Alessio Bonaldo 《Aquaculture Research》2011,42(3):313-321

This study was undertaken to determine the influence of dietary protein levels on growth, feed utilization and liver histology in common sole. Four isoenergetic diets were formulated to contain four different crude protein levels: 39 (P39), 45 (P45), 51 (P51) and 57 (P57) % dry weight. Fifty animals per tank (initial weight 10.2 ± 0.4 g) were randomly distributed into twelve 500 litre square tanks (bottom surface: 5600 cm²) connected to a closed recirculation system. The diets were tested in triplicate for 84 days. At the end of the experiment, the final weight ranged from 19.6 (P39) to 25.4 g (P57). The specific growth rate showed statistical differences between groups, with the best results in the group fed diet P57 (1.07% day^?1). Significant differences between groups were also recorded for the feed conversion ratio, with values of 1.31, 1.28, 1.12 and 0.94 in P39, P45, P51 and P57 respectively. Gross lipid efficiency was also significantly affected by the dietary treatment, with the highest value (42.07%) found in P57. Ammonia excretion, expressed as g 100 g^?1 protein intake, was significantly lower for group P39 (2.46) than groups P51 (4.70) and P57 (4.75). Increased incidence of lipid droplets in hepatocytes was observed when the dietary protein levels increased and/or dietary lipid decreased.  相似文献   

Fasting and Refeeding Affect the Goose Liver Transcriptome Mainly Through the PPAR Signaling Pathway     

Zhenzhen Chen  Ya Xing  Xue Fan  Tongjun Liu  Minmeng Zhao  Long Liu  Xuming Hu  Hengmi Cui  Tuoyu Geng  Daoqing Gong 《The Journal of Poultry Science》2021,58(4):245

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香菇多糖对力竭小鼠抗疲劳及保肝作用研究   总被引：10，自引：0，他引：10  

史亚丽  辛晓林  杨立红  蔡德华 《吉林农业大学学报》2004,26(3):301-304

为研究香菇多糖对力竭后小鼠肝组织的影响,通过建立灌服香菇多糖的小鼠力竭游泳实验模型,采用试剂盒法测定了小鼠安静时肝糖元及安静与力竭后谷胱甘肽过氧化物酶(GSH PX)、丙二醛(MDA)、血浆谷丙转氨酶(GPT)活性。结果显示:肝糖元,安静对照组(RC)(36 93±9 81)mg g,安静服药组(RM)(73 44±21 71)mg g,RM组明显升高(P<0 001);高强度游泳时间,运动对照组(EC)(42 52±7 82)min,运动服药组(EM)(49 90±5 31)min,EM组明显延长(P<0 05);GSH PX,RC组(60 25±5 31)U mg,RM组(68 37±7 92)U mg,RM组明显升高(P<0 05)。EC组(56 77±7 30)U mg,EM组(63 28±4 23)U mg,EM组明显升高(P<0 05);MDA水平,RC组(5 76±0 87)mmol L,RM组(4 59±0 57)mmol L,RM组明显降低(P<0 05)。EC组(14 1±1 28)mmol L,EM组(12 53±4 93)mmol L,EM组明显降低(P<0 01);GPT活性,RC组(6 14±1 21)U L,RM组(6 25±1 02)U L,差异不显著(P>0 05)。EC组(12 53±4 93)U L,EM组(7 42±2 90)U L,EM组明显降低(P<0 05)。表明香菇多糖具有明显的抗疲劳、抗氧化、抗损伤功效,可减轻大强度运动后自由基造成的损伤。  相似文献   

Influence of Pesticides on UDP-glucuronosyltransferase in Rat Liver Microsomes     

Zlatina Naydenova  Zoltan Urshev  Evgeny Golovinsky  Konstantin Grancharov 《Pest management science》1996,47(1):25-30

Fifteen pesticides, representatives of different chemical groups, were tested for their inhibitory effect on the glucuronidation of 4-nitrophenol (4-NP) and phenolphthalein (PPh) by rat liver microsomes. Three herbicides (simazine, chlorsulfuron, tribenuron-methyl), two insecticides (dioxacarb, carbaryl) and one fungicide (zineb) significantly decreased the UDP-glucuronosyltransferase (UDPGT) activity. The carbamate insecticide dioxacarb was found to be the most potent inhibitor, at 1 mM concentration suppressing 4-NP-UDPGT activity completely, and reducing by 55% the activity associated with the conjugation of PPh. One millimole simazine and carbaryl affected only 4-NP glucuronidation, while chlorsulfuron and zineb exerted a marked inhibition of both 4-NP and PPh conversion. Concentrations of 0·1 mM carbaryl, dioxacarb and zineb were still inhibitory against 4-NP-UDPGT, with zineb producing 40% inhibition of PPh glucuronidation. As a whole, UDPGT isoforms conjugating PPh were less sensitive to the agrochemicals tested. Kinetic studies with dioxacarb, chlorsulfuron and carbaryl revealed a mixed type of inhibition with respect to the acceptor substrate 4-NP, with apparentK_i values of 70 μM , 120 μM and 160 μM , respectively.  相似文献   

The effect of ultrafiltered fish protein hydrolysate levels on the liver and muscle metabolic profile of juvenile turbot (Scophthalmus maximus L.) by 1H NMR‐based metabolomics studies          下载免费PDF全文

Yuliang Wei  Mengqing Liang  Kangsen Mai  Keke Zheng  Houguo Xu 《Aquaculture Research》2017,48(7):3515-3527

A ¹H NMR‐based metabolomics approach was to explore the effect of ultrafiltered fish protein hydrolysate (UF) levels on the liver and muscle metabolic profile of juvenile turbot (Scophthalmus maximus L.). Fish protein hydrolysate (FPH) was produced from by‐products by enzymatic treatment, and UF was obtained by diluting FPH followed filtration. Fish were fed diets containing fish meal protein, which was, respectively, replaced by UF protein 0, 50, 100, 150 and 200 g kg^?1 of dietary total protein (UF‐0, UF‐5, UF‐10, UF‐15 and UF‐20) for 68 days. OPLS‐DA of liver and muscle showed that high levels of UF in diets may lead to corresponding tissue metabolites changes. In liver tissue, changing metabolites included dimethylamine, N,N‐dimethylglycine, valine, isoleucine, leucine, arginine and alanine and were mainly involved in choline metabolism and amino acid metabolism. For muscle tissue, changing metabolites included lactate, alanine, proline, fumarate, tyrosine, histidine, cystathionine and taurine. And the metabolites were mainly involved in glycolysis and gluconeogenesis, proline metabolism and taurine metabolism. ¹H NMR‐based metabolomics is a useful approach to investigate different levels of UF on metabolic profile in liver and muscle tissues combined with the growth.  相似文献   

Differential expression of lipid metabolism‐related genes and miRNAs in Ctenopharyngodon idella liver in relation to fatty liver induced by high non‐protein energy diets          下载免费PDF全文

Jun‐li Wang  Rong‐hua Lu  Jun‐jun Sun  Di‐zhi Xie  Feng Yang  Guo‐xing Nie 《Aquaculture Research》2017,48(8):4070-4085

To explore the mechanism of fatty liver formation induced by high non‐protein energy diets in grass carp (Ctenopharyngodon idella), basal diet and high‐energy diets were fed to juvenile grass carp for 9 weeks. The experimental groups fed on high‐energy diets which included a high‐lipid diet (H‐LIP), a high‐carbohydrate diet (H‐CHO) and a high‐lipid and carbohydrate diet (H‐CL). The control group fed on basal diet. Growth performance, liver fat accumulation, serum biochemical indexes and the expression levels of lipid metabolism‐related genes (SREBP‐1, PPARγ, FAS, ACC1, and LPL) and miRNAs (miR‐33, miR‐122, and miR‐370) were examined at the end of the feeding trial. There were no significant differences in growth rate and feed efficiency among the four groups. However, significant increase in mesenteric and liver fat contents, and lipid droplets in the liver was induced by high‐lipid and high‐carbohydrate diets. There were significant differences in serum biochemical indicators such as AST/ALT, GLB, TG and TP, and liver fatty acid composition between the control and experimental groups. The expression levels of SREBP‐1, PPARγ, FAS, ACC1 and LPL were upregulated, while CPT‐1 was downregulated with the high‐energy treatments. Additionally, the expression levels of miR‐33, miR‐122 and miR‐370 in the liver were higher in the three high‐energy treatments than those in the control (P < 0.05). The results suggest that modifications of lipid metabolism‐related genes and miRNAs may be involved in fatty liver formation induced by high non‐protein energy diets in grass carp.  相似文献   

哲罗鱼肝脏和胰脏发生和发育的组织学研究     

关海红  尹家胜 《鲑鳟渔业》2011,(3):16-20

采用形态观察与连续组织切片技术,观察和研究了哲罗鱼（Hucho tai men）肝脏和胰脏在胚胎期（水温7～8℃）和胚后期（水温3～14℃）的发育。结果表明,与大多数硬骨鱼类不同,哲罗鱼的肝脏是在破膜前就开始发生、发育,而胰脏是在破膜后出现,以后逐渐发育完善。受精18 d肝原基细胞出现,破膜8 d,胰细胞出现,破膜8 d肝后部开始贮存脂质,破膜18 d后肝前部开始积累脂质,肝脏发育与卵黄囊有密切的关系。肝脏和胰脏为相互分开的独立器官,具有丰富的肝、胰管。  相似文献   

败血症鲢肝与肾cDNA文库构建及MHC classⅠ的克隆与分析     

汪登强  罗晓松陈大庆 《中国水产科学》2011,18(6)

采用CloneMinerTM cDNA文库构建试剂盒构建了患败血症的鲢(Hypophthalmichthys molitrix)肝和肾的cDNA文库.经检验,文库的滴度为1.34×107 cfu/mL,总库容为2.68×107 cfu,阳性克隆率为97.5％,平均插入片段大于1.2 kb.对80个随机挑选的克隆进行两端测序,结果显示有74个测序成功.经在GenBank上BLAST比对,其中57个为已知功能基因,属于45个不同基因,另外17个没有明显的同源序列.在已知功能的57个克隆中,含有全长编码区序列的克隆共有49个,全长cDNA比率为61.25％.采用PCR法对文库进行筛选,获得一个含MHC classⅠ完整编码区的序列,该序列具有典型的脊椎动物MHC class Ⅰ的序列特征.本研究完成鲢肝和肾较高质量的cDNA文库的构建,旨在为今后开展鲢功能基因研究奠定基础.  相似文献   

不同毒物对猪肝线粒体COX和Ca2+-ATPase同工酶的影响   总被引：4，自引：0，他引：4  

梅启明  郭鹏  周培疆  刘义 《湖北农业科学》2004,(3):89-91

采用不同毒物联苯胺、六氯苯、甲基苯丙胺对猪肝线粒体进行体外染毒,分析细胞色素氧化酶(COX)和钙ATP酶(Ca2 -ATPase)同工酶。结果表明,这两种线粒体酶同工酶受联苯胺和甲基苯丙胺的影响是同步的;受六氯苯的影响表现为COX反应提前和Ca2 -ATPase反应滞后。不同毒物对线粒体COX和Ca2 -ATPase毒性作用可能为一种混合机制。  相似文献   

200例食用猪肝的病理学研究     

杨守凯  陈玉汉  朱雄 《中国动物检疫》1999,16(4):5-7

对取自当前广州市肉品市场的200例食用猪肝进行了病理学分析,68％猪肝存在着各种各样的病变,病变种类多达25种,不仅有肝细胞的萎缩和各种变性、水肿、囊肿、出血、坏死和钙化等,还发现恶性肿瘤以及见于癌前期或癌症的肝细胞病理性核有丝分裂像。  相似文献