青海省民和县土地利用景观生态格局动态分析     

冶晓铮  张金旭  候智国 《农学学报》2023,13(5):50-57

为研究青海省民和县近20年景观分布规律及其土地利用程度问题,在GIS技术的支持下,将青海省民和县作为研究对象,选用2000、2010、2020年的全球地表覆盖数据GlobeLand30,基于GlobeLand30分类系统,构建分类体系,并通过土地类型转移矩阵得到20年来研究区各景观类型面积及其变化情况,最后利用Fragstats4景观分析软件选取9个主要景观指标,对民和县的景观格局进行斑块类型和景观水平的对比分析。结果表明：（1）面积增加最多的是人造地表,耕地面积减少最多,耕地转化为人造地表的趋势尤为明显;（2）草地和耕地的破碎化程度最高,湿地破碎化程度较小;（3）土地利用程度指数整体上升,土地利用效果明显。进行景观生态格局动态分析对区域的土地利用规划有重要的作用,可为其生态环境保护提供参考。  相似文献   

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella     

Xingliang Wang  Yiming Ma  Falong Wang  Yihua Yang  Shuwen Wu  Yidong Wu 《Pest management science》2020,76(5):1618-1625

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Evaluation of cultural control and resistance‐breeding strategies for suppression of whitefly infestation of cassava at the landscape scale: a simulation modeling approach     

Hazel Parry  Andrew Kalyebi  Felix Bianchi  Peter Sseruwagi  John Colvin  Nancy Schellhorn  Sarina Macfadyen 《Pest management science》2020,76(8):2699-2710

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Embryo-mediated genome editing for accelerated genetic improvement of livestock     

Zachariah MCLEAN  Bj&#  rn OBACK  G&#  tz LAIBLE 《农业科学与工程前沿(英文版)》2020,7(2):148-160

Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.  相似文献   

Proboscipedia and Sex combs reduced are essential for embryonic labial palpus specification in Bombyx mori          下载免费PDF全文

ZHANG Ru  ZHANG Zhong-jie  YU Ye  HUANG Yong-ping  QIAN Ai-rong  TAN An-jiang 《农业科学学报》2020,19(6):1482-1491

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Identifying and Characterizing a Novel Peritrophic Matrix Protein (MdPM-17) Associated With Antibacterial Response From the Housefly,Musca domestica (Diptera: Muscidae)     

Yu Wang  Jinzhi Cheng  Man Luo  Jianwei Wu  Guo Guo 《Journal of insect science (Online)》2020,20(6)

Peritrophic matrix/membrane (PM) critically prevents the midgut of insects from external invasion by microbes. The proteins in the peritrophic membrane are its major structural components. Additionally, they determine the formation and function of this membrane. However, the role of PM proteins in immune regulation is unclear. Herein, we isolated a novel PM protein (MdPM-17) from Musca domestica larvae. Further, the function of MdPM-17 in regulating host innate immunity was identified. Results showed that the cDNA of MdPM-17 full is 635 bp in length. Moreover, it consists of a 477-bp open reading frame encoding 158 amino acid residues. These amino acid residues are composed of two Chitin-binding type-2 domain (ChtBD2) and 19 amino acids as a signal peptide. Moreover, tissue distribution analysis indicates that MdPM-17 was enriched expressed in midgut, and moderate levels in the fat body, foregut, and malpighian tubule. Notably, MdPM-17 recombinant protein showed high chitin-binding capacity, thus belongs to the Class III PM protein group. MdPM-17 protein silencing via RNA interference resulted in the expression of antimicrobial peptide (defensin, cecropins, and diptericin) genes, and this occurred after oral inoculation with exogenous microbes Escherichia coli (Enterobacteriales:Enterobacteriaceae), Staphylococcus aureus (Bacillales:Staphylococcaceae), and Candida albicans (Endomycetales:Saccharomycetaceae)). Therefore, all the antimicrobial peptide (AMP) gene expression levels are high in MdPM-17-depleted larvae during microbial infection compared to controls. Consequently, these findings indicate that MdPM-17 protein is associated with the antibacterial response from the housefly.  相似文献   

Down-regulation of miR-153-3p attenuates H₂O₂-induced H9C2 cell injury by promoting Nrf2 expression     

HUANG Zhao-qi  XU Wei  HUANG Jiong-hua  LU Xiong 《园艺学报》2020,36(7):1249-1254

AIM To study the effect of microRNA-153-3p (miR-153-3p) knock-down on oxidative injury of H9C2 cells induced by H₂O₂ and its specific mechanism. METHODS The oxidative stress injury of H9C2 cell model was induced by H₂O₂, and then the cell viability and the expression of miR-153-3p were detected by MTT assay and RT-qPCR, respectively. The effects of miR-153-3p knock-down on the H9C2 cell injury under oxidative stress were studied by RNA interference technology. The targets of miR-153-3p were identified by Western blot and dual-luciferase reporter assay. RESULTS MTT assay showed that the viability of H9C2 cells was decreased with the increase in H₂O₂ concentration (P<0.05). The results of RT-qPCR showed that the expression of miR-153-3p was increased with the increase in H₂O₂ concentration (P<0.05). Knock-down of miR-153-3p increased the viability of H9C2 cells under oxidative stress, decreased the cell apoptosis and the content of malondialdehyde (MDA), and increased the activity of superoxide dismutase (SOD). The expression of nuclear factor E2-related factor 2（Nrf2） and antioxidant response element（ARE） activity were increased with the increase in H₂O₂ concentration (P<0.01). TargetScan analysis and dual-luciferase reporter assay showed that Nrf2 was one of the potential target genes of miR-153-3p. The results of Western blot further showed that over-expression of miR-153-3p inhibited the expression of Nrf2 (P<0.01), while down-regulation of miR-153-3p increased the expression of Nrf2 (P<0.01). Dual interference with Nrf2 and miR-153-3p significantly reduced H9C2 cell viability, promoted the apoptosis, increased MDA content, and decreased SOD activity in the presence of H₂O₂ (P<0.01). CONCLUSION Inhibition of miR-153-3p expression attenuates the injury of H9C2 cells induced by H₂O₂ through up-regulating Nrf2/ARE signaling pathway.  相似文献   

利用CRISPR/Cas9系统敲除葡萄中VviEDR2提高对白粉病的抗性     

杨禄山  郭晔  胡洋  文颖强 《园艺学报》2020,47(4):623-634

利用CRISPR/Cas9系统定点编辑葡萄白粉病感病基因VviEDR2（Enhanced disease resistance 2）,在VviEDR2的DUF1336结构域设计靶位点VviEDR2-T1,构建CRISPR/Cas9敲除载体,通过农杆菌介导法转化‘无核白’葡萄胚性愈伤组织。对PCR阳性植株进行靶位点扩增测序,结果表明,共有8个转基因植株在靶位点处发生不同类型的双等位基因突变,编辑效率为32%;突变体植株生长势较弱,叶片较小,茎秆丛生、细弱。进一步对突变体植株进行抗病检测,结果表明,接种葡萄白粉菌（Erysiphe necator Schw.）5 d后,突变体植株叶片上白粉菌孢子仅能萌发出少量较短初级菌丝,表皮细胞产生大量明显的H2O2,而野生型叶片中白粉菌萌发出大量初级菌丝、次级菌丝和吸器,无明显H2O2产生。这些结果表明,可以利用CRISPR/Cas9技术编辑葡萄感病基因VviEDR2,提高葡萄白粉菌抗性。  相似文献   

太子参须提取物对鸭流感病毒体外试验初探   总被引：1，自引：0，他引：1  

张炎达  施少华  潘慧青  杨荣平  赵齐 《福建畜牧兽医》2021,(2):9-10

为尝试性探究太子参须提取物对鸭H9N2亚型流感病毒体外生长的抑制作用,本文基于TCID50试验检测鸭H9N2亚型流感病毒体外感染不同处理组的MDCK细胞.结果 表明,太子参须提取物高低剂量组(75％、25％)和黄芪多糖阳性对照组均对鸭H9N2亚型流感病毒体外生长产生抑制,且太子参须提取物高剂量组(75％)的抑制作用最优...  相似文献   

黄栌大棚轻基质育苗及嫩枝扦插育苗技术研究   总被引：1，自引：0，他引：1  

李贵芬  刘朝华  高鹏 《现代农业科技》2020,(5)

黄栌是我国重要的观赏红叶树种,也是优良的造林树种。黄栌大棚轻基质育苗,可节约时间、空间,操作方便快捷,有利于管理。嫩枝扦插育苗繁殖速度快,生产成本低,有利于大面积推广。本研究用不同激素浓度处理黄栌插穗,结果表明,用400 mg/L IBA溶液处理黄栌插穗,插穗的生根率最高(81.96%),生根效果最好。该文详细介绍了黄栌大棚轻基质育苗及嫩枝扦插育苗技术,以利于提高黄栌大棚轻基质育苗及嫩枝扦插育苗的成活率,为培育优质黄栌苗木提供参考。  相似文献