Effect of Lysine Supplement on the Digestion and Metabolism of the Rumen in Small-tail Han Sheep     

LIU Wen-jie  LUO Qiu-jiang  ZHONG Tao  CHEN Yong  PAN Rong 《中国畜牧兽医》2015,42(10):2658-2668

5 male Small-tail Han sheep with the body weight of 45 kg at 1.5 years old,fitted with permanent rumen fistula,to study of the effect of the supplement of the untreated commercial lysine on the rumen microbiocoenosis,rumen metabolism and whole body metabolism by 5×5 Latin square design (5 groups:control,control with low urea (equal nitrogen),low lysine,control with high urea (equal nitrogen),high lysine).The amounts of lysine-HCl supplement were 0,4.0 and 8.0 g/kg diet (as DM basis) in each group respectively,with two urea supplement control groups of equal amount of nitrogen.The results showed that by supplement of 4.0 and 8.0 g/kg of lysine-HCl diet (as DM basis) respectively,the voluntary intake of dry matter of sheep were increased by 5.5%(P>0.05) and 11.8%(P<0.05) respectively.The total amount of rumen bacteria were increased by 18.9%(P<0.01) and 23.9%(P<0.01) respectively,as the amount of rumen Coccus was increased by 21.2%(P<0.01) and 30.1%(P<0.01) respectively.The amount of rumen big Bacillus was decreased by 16.7%(P<0.05) and 33.3%(P<0.01) respectively.The total VFA of was increased by 9.6%(P<0.05) and 12.2%(P<0.01) respectively,as the butyric acid was increased by 12.8%(P>0.05) and 20.2%(P<0.01) respectively.The apparent digestibility of organic matter was increased by 6.4%(P>0.05) and 10.0%(P<0.05) respectively,the apparent digestibility of crude protein was increased by 7.0%(P>0.05) and 18.3%(P<0.01) respectively;And nitrogen retention was increased by 46.4%(P>0.05) and 110.7%(P<0.01) respectively.However,there was no effect of lysine supplement on the amounts of the rumen protozoa and fungi,and the concentration of rumen ammonia nitrogen.It was concluded that the supplement of untreated commercial lysine to adult sheep would increase the voluntary intake,digestibility,nitrogen retention the rumen microbiocoenosis and the total amount of rumen bacteria,especially the rumen Coccus of adult sheep.The effect of lysine supplement could not be replaced by urea supplement completely.  相似文献   

胚胎冻融技术及其在我国肉羊产业中的应用     

特日格勒  ;何小龙  ;王孟巍  ;祁云霞  ;刘永斌  ;荣威恒 《畜牧与饲料科学》2014,(7):53-56

胚胎冻融技术可使胚胎移植不受时间、地点的限制，从而达到提高胚胎移植效率的目的，研究和优化胚胎冻融技术对胚胎移植工程的发展具有重要的意义。主要对胚胎冻融技术的原理、特点及其在肉羊产业中的应用情况进行了综述，旨在阐明胚胎冻融技术对我国肉羊产业发展的重要作用。  相似文献   

Comparative Study on Carcass Traits and Meat Quality of Different Month Old of Xinjiang Brown Cattle Steers     

YAN Xiang-min  ZHANG Jin-shan  LI Hong-bo  LI Na  DU Wei  ZHOU Zhen-yong  ZHANG Yang 《中国畜牧兽医》2015,42(11):2954-2960

In this research,12 heads of 28 to 30 months (low months age group) and 8 heads of 32 to 34 months (high months age group) Xinjiang Brown cattle steers which fed in the same mode of nutrition were chose for the slaughter test,in which the carcass traits,nutrient composition and some edible quality index were measured and comparative analyzed,a research which through comparative analysis the carcass traits and meat quality of nutritional components of different months of Xinjiang Brown cattle steers was carried out.The results indicated that the marbling,carcass grade,fat,meat color of high months age group were better than that of low months age group,the traits of the low months age group on fat color,moisture,protein,cooking loss and shear force value were better than that of high months age group,and there were significant difference on the traits of fat and meat color L^* between the two groups (P<0.05),while the differences of other traits between them were not significant (P>0.05);As high-grade beef,in addition to the traits of meat color and pH value of the chunk roll,tenderloin and striploin,each site of the meat traits on the fat,protein,moisture,cooking loss,shear force value indicators were all better than other parts,and showed the best of nutritional value and edible quality.28 to 34 months of Xinjiang Brown cattle steers could produce high-grade beef,the meat could be used as the raw materials of steak and boiled beef.  相似文献   

绵羊FGF10基因克隆、序列分析及其在毛囊发育中的表达分析     

陈磊  袁枫  贺三刚  玛依拉  李文蓉 《中国畜牧兽医》2021,48(2):425-432

本试验旨在获得中国美利奴羊成纤维细胞生长因子10（fibroblast growth factor 10，FGF10）基因的编码区（CDS）全长序列并进行生物信息学分析，随后对FGF10基因在中国美利奴羊毛囊发育过程中的表达特征进行分析，明确其在中国美利奴羊毛囊发育过程中的表达模式，为进一步研究FGF10 mRNA表达水平与中国美利奴羊毛囊生长发育的表达调控机制奠定理论基础。采用PCR扩增获得中国美利奴羊FGF10基因CDS，并克隆到zero PCR@^TM-Blunt进行测序验证；利用实时荧光定量PCR技术检测FGF10在中国美利奴羊毛囊发育过程中的表达差异。结果表明，绵羊FGF10基因CDS长度为696 bp（序列上传GenBank，获得登录号：MT872422），编码231个氨基酸，与牛和山羊的氨基酸序列同源性达100%，存在1个信号肽和1个跨膜结构域，其为分泌通路信号蛋白；实时荧光定量PCR分析表明，FGF10基因在中国美利奴羊毛囊发育过程中均表达，在毛囊发育第85天表达最高，显著高于其他毛囊发育时期（P<0.05）。本研究获得中国美利奴羊FGF10基因完整的编码区序列和毛囊发育过程中的表达特征，生物信息学分析发现，FGF10基因编码区序列具有物种间的保守性，同时FGF10在绵羊毛囊不同发育阶段的皮肤组织中表达，由此表明，FGF10基因可能在绵羊毛囊的生长发育过程中发挥重要的生物学作用。  相似文献   

Bioinformatics Analysis of Bone Morphogenetic Protein 4 (BMP4) of Sheep     

FAN Zhen-hua  ZHANG Qiu-yue  FAN Rui-wen  REN Yu-hong 《中国畜牧兽医》2015,42(6):1370-1376

The assay was aimed to explore the biological characteristics of bone morphogenetic protein 4 (BMP4) of sheep,NCBI,DNAMAN DNAStar,TMHMM Server v.2.0,PsortⅡ,SignalP various bioinformatical softwares were used to speculate the physical and chemical properties,hydrophobic property,phosphorylation site,conservative structure domain,protein secondary structure of BMP4 protein.Also,the three-dimensional structure was forecasted with the SWISS-MODEL Workspace software.The results indicated that the BMP4 of sheep had high homologies with the BMP4 of various species.The encoded protein was a hydrophilic protein which was unstable.There was no transmembrane regions and it was likely to be located in the nucleus.What was more,there was signal peptide and eighteen phosphorylation sites.Through the forecast of functional domains,the protein had two functional domains,including the transforming growth factor-beta (TGF-beta) superfamily and TGF-beta propeptide superfamily.The result was consistent with the function of BMP4 gene family,it also demonstrated that BMP4 was a growth factor and it had the function of signal transduction.The amino acid homology between the predicted 3D structure of protein and template 3bmp.1.A was 88.29%.The bioinformatics analysis of BMP4 gene could provide reference for the further study in practice.  相似文献   

日粮钙水平对空怀期云南半细毛羊生长性能及血清生化指标和繁殖相关激素的影响     

《中国畜牧杂志》2019,(3)

试验旨在探讨日粮钙水平对空怀期云南半细毛羊生长性能、配种前血清生化指标和繁殖相关激素的影响,为开展空怀期云南半细毛羊营养需要量研究提供参考。选用50只体况良好、体重相近、38月龄的经产2胎空怀云南半细毛羊,随机均分为5组,分别饲喂0.40%(无外源钙)、0.45%、0.53%、0.66%、0.85%5个钙水平的日粮。预试期14d,正试期30d。结果表明:0.53%钙水平组云南半细毛羊的总增重、平均日增重、平均日采食量、耗料增重比等指标相对优于其他组;0.40%钙水平日粮组的血清钙最高,血清碱性磷酸酶、甲状旁腺素、钙、磷组间差异不显著(P>0.05);繁殖相关激素各组间表现不一,0.40%钙水平组雌二醇水平最高(P>0.05)。在日粮磷水平为0.20%水平条件下,0.53%钙水平组的空怀期云南半细毛羊表现出较好的生长性能,可为临配种前生理生化和激素水平提供较好准备;增加日粮钙水平,未见对以上指标有明显的正向增强作用,可能与日粮钙磷比失衡有关。  相似文献   

阿勒泰羊生长速度与主要血液生化指标含量及尾脂Leptin基因表达关系     

麦麦提依明&#  麦麦提力  叶梦珺  候萌  王俊丽  唐安琪  向微微  柳荦  王金泉  胡燕 《中国畜牧兽医》2021,48(7):2369-2377

试验通过研究不同生长速度阿勒泰羊主要血液生化指标含量和尾脂中瘦素（Leptin）基因的表达水平，探讨阿勒泰羊生长速度与脂肪沉积之间的关系。选取300只出生时间相近的阿勒泰羊公羔，正常饲喂至6月龄，记录日增重，取平均日增重排前10%和后10%的公羔各30只，分为生长快速组（HBW）和生长慢速组（LBW），从生长快速组随机选12只分为抑制生长组（饲喂低能量饲粮以抑制其生长，HBW-75%，n=6）和生长快速对照组（饲喂标准饲粮，HBW-100%，n=6），生长慢速组随机选12只分为促进生长组（饲喂高能量饲粮以促进生长，LBW-125%，n=6）和生长慢速对照组（饲喂标准饲粮，LBW-100%，n=6）；预饲期7 d，正饲期30 d，试验结束后称重并采集血液，屠宰后采集尾部脂肪。检测血液TG、CK、TSH、T3、T4、S.S、GH、INS、PG、Leptin含量或活性及尾部脂肪Leptin mRNA和蛋白表达量，并观察尾部脂肪细胞形态学变化。结果显示，HBW-100%和LBW-100%组初体重与末体重均差异显著（P<0.05），HBW-75%和HBW-100%组初体重与末体重均无显著差异（P>0.05）；LBW-100%和LBW-125%组初体重差异显著（P<0.05），末体重无显著差异（P>0.05）。LBW-100%组的T4、S.S、Leptin、TSH、T3含量显著低于HBW-100%组（P<0.05）；LBW-125%组T3含量显著高于LBW-100%组（P<0.05）；HBW-75%组GH、PG和Leptin含量显著低于HBW-100%组（P<0.05）。LBW-125%组尾部脂肪细胞面积大于LBW-100%组，HBW-75%组小于HBW-100%组（P<0.05）。Leptin mRNA和蛋白表达量检测结果表明，HBW-100%组的Leptin mRNA表达量与LBW-100%组无显著差异（P>0.05），Leptin蛋白表达量极显著高于LBW-100%组（P<0.01）。LBW-125%组Leptin mRNA和蛋白的表达量均显著高于LBW-100%组（P<0.05）。HBW-75%组Leptin mRNA的表达量极显著低于HBW-100%组（P<0.01），Leptin蛋白表达量显著低于HBW-100%组（P<0.05）。综上所述，生长快速组与生长慢速组中，Leptin的表达量与体重和体脂有一定关联；在生长慢速组促进生长后体重和体脂显著增加，Leptin的表达量呈上升趋势；在生长快速组抑制生长后体重和体脂减少，Leptin的表达呈下降趋势。改变生长速度对Leptin的表达量会产生影响，从而改变机体的脂肪沉积状况。  相似文献   

鲜活农产品在连云港市的物流与营销     

王世法  罗林花  于建芝 《绿色科技》2011,(10)

指出了人们对农产品的消费观念发生了深刻的变化，从消费者对农产品的需求及消费者购买行为两方面分析了连云港市的市场环境，并对鲜活农产品在连云港市的物流与营销进行了探讨。  相似文献   

Effect of Alfalfa Flavonoids on Growth Performance and Serum Indexes of Sheep     

WANG Meng-zhu  LIU Yan-feng  WANG Wen-qi  NIE Cun-xi  TANG Shu-zhen  ZHANG Wen-ju 《中国畜牧兽医》2015,42(9):2345-2351

This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%.  相似文献   

家畜养殖生产的环境效应研究——基于浙江省67个县市的农业经济截面数据     

姚文捷  王学渊 《湖北农业科学》2016,(20):5403-5407

利用浙江省67个县市的农业经济截面数据,以环境负荷量为因变量,以肉产量和耕地面积为自变量构建模型,对家畜养殖生产的环境效应进行研究。结果表明,2013年绍兴市区、衢州市区、龙游县和江山县的环境负荷量超过警戒值,金华市区、德清县和淳安县的环境负荷量超过适宜值;家畜养殖生产存在着环境负效应,并受到耕地资源的约束,其环境负荷量与肉产量正相关,与耕地面积负相关。  相似文献