FGF21及其受体FGFR1和FGFR2在小鼠毛囊第1生长周期的表达     

吴晋强  曹校瑞  闫瑞琴  陆娜  张娇娇  吴佳豪  赫晓燕 《畜牧兽医学报》2019,(3):534-543

旨在通过对FGF21、受体FGFR1和FGFR2在小鼠毛囊第1生长周期中的定位和表达情况的研究,以探索FGF21、受体FGFR1和FGFR2在毛囊发育过程中的作用。选取1、3、5、8、12、17、21和23日龄小鼠背部皮肤,采用免疫组织化学技术,实时荧光定量PCR和蛋白免疫印迹技术检测FGF21、FGFR1和FGFR2 mRNA及蛋白表达。结果显示:在小鼠毛囊第1生长周期中,FGF21主要表达于毛囊毛乳头、毛基质、内外根鞘以及毛囊周围的结缔组织中;FGFR1在毛囊各部均有表达,且在退化期(12～17日龄)和静止期(18～21日龄)主要表达于内外根鞘中;FGFR2广泛表达于毛囊各部。1、3、5、8、12、17日龄FGF 21 mRNA表达量极显著低于23日龄(P<0.01),21日龄的表达量低于23日龄,差异不显著(P>0.05);1～5日龄和23日龄FGF21蛋白表达量较高。1、3、5、8、12、21、23日龄FGFR1 mRNA表达量极显著低于17日龄(P<0.01);FGFR1蛋白表达量从12日龄开始上升,在17日龄时达到最高,之后又呈下降趋势。1、5、8、12、21和23日龄FGFR2 mRNA表达量极显著低于17日龄(P<0.01),在3日龄的表达量低于17日龄,差异不显著(P>0.05);FGFR2蛋白在1～17日龄都有较高表达。综上表明,在第1个毛囊生长周期中,FGF21对参与毛囊形成的成纤维细胞的增殖和分化具有重要作用,诱导毛囊由生长期进入退化期。FGFR1对内外根鞘细胞的增殖、分化有着明显作用,诱导毛囊由退化期进入静止期。FGFR2对毛囊内细胞增殖、分化具有重要作用,诱导毛囊由生长期和退化期进入静止期。  相似文献   

miR-18a通过靶向结合CTGF调控猪颗粒细胞凋亡   总被引：1，自引：0，他引：1  

郭天亚  黄龙  马梦楠  姚望  潘增祥 《畜牧与兽医》2019,(7):13-18

为探究miR-18a对猪卵巢颗粒细胞凋亡的调控作用,利用生物信息学分析、荧光素酶活性检测和体外培养颗粒细胞试验验证miR-18a对CTGF的靶向作用及其在猪颗粒细胞中对CTGF基因表达的影响,并通过流式细胞技术检测其对猪卵巢颗粒细胞凋亡的调控作用。生物信息学分析结果表明,CTGF是miR-18a的潜在靶基因,荧光素酶活性检测进一步验证了miR-18a与CTGF的结合。在培养的颗粒细胞中转染miR-18a模拟物后,qRT-PCR和Western blot结果显示CTGF的mRNA和蛋白水平均显著降低,流式细胞技术检测表明miR-18a显著促进颗粒细胞的凋亡。而转染miR-18a抑制剂后,猪颗粒细胞的凋亡率显著降低,共转染miR-18a抑制剂和CTGF的干扰RNA后,颗粒细胞的凋亡率呈现回升。试验结果表明miR-18a通过靶向结合CTGF基因调控猪颗粒细胞的凋亡。  相似文献   

Over-expression of P21 attenuates cisplatin-induced HK-2 cells injury     

CAO Lei  ZHENG Zhuo  LI Ning  ZHAO Xun  ZHANG Ying-xuan 《园艺学报》2018,34(7):1239-1244

AIM:To investigate the effect of P21 on cisplatin-induced renal tubular epithelial cells injury.METHODS:The expression of P21 at mRNA and protein levels in cisplatin treated human renal tubular epithelial cells (HK-2) cells was determined by RT-qPCR and Western blot. Over-expression of P21in the HK-2 cells was induced by the transfection of pcDNA3-P21. The cell viability and cell apoptosis were detected by CCK-8 assay and flow cytometry, respectively. Furthermore, the protein expression of kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), caspase-3, glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP), and phosphorylation level of eucaryotic translation initiation factor 2α (eIF2α) and protein kinase R-like endoplasmic reticulum kinase (PERK) were detected by Western blot.RESULTS:Cisplatin increased the mRNA and protein levels of P21 in a time-and concentration-dependent manner in the HK-2 cells. Over-expression of P21 inhibited cisplatin-induced cell apoptosis, and down-regulated the expression of KIM-1 and NGAL. Furthermore, Over-expression of P21 decreased the protein levels of GRP78, p-PERK, p-eIF2α, CHOP and cleaved caspase-3.CONCLUSION:Over-expression of P21 attenuates cisplatin-induced HK-2 cells injury, and the mechanism may be related to the modulation of endoplasmic reticulum stress pathway and inhibition of cell apoptosis.  相似文献   

植物根际促生菌普城沙雷菌A21-4对黄瓜生长及土壤微生态的影响     

丁方丽  李嫚  刘东平  张忠良  孙治强  朴凤植  申顺善 《中国蔬菜》2018,1(5):36-41

为探讨植物根际促生菌普城沙雷菌A21-4在黄瓜上的应用效果,将A21-4菌悬液灌注到黄瓜根际土壤,调查其对黄瓜生长发育和根际土壤微生态的影响。结果表明,根部灌注A21-4能显著促进苗期和田间生育期黄瓜生长发育,并显著提高根系活力、产量和品质,黄瓜产量比对照增加32.22%,且黄瓜果实中蛋白质、可溶性糖和VC含量均比对照显著增加;同时,A21-4对黄瓜根际土壤具有明显的调节作用,显著提高了黄瓜根际土壤脲酶、磷酸酶、蔗糖酶和过氧化氢酶活性,还提高了黄瓜根际土壤细菌和放线菌的数量及黄瓜根际土壤速效氮、磷、钾的含量。  相似文献   

MicroRNA‐21 and microRNA‐214 play important role in reproduction regulation during porcine estrous          下载免费PDF全文

Mi Tian  Xu Zhang  Pengfei Ye  Qiangqiang Tao  Liang Zhang  Yueyun Ding  Mingxing Chu  Xiaodong Zhang  Zongjun Yin 《Animal Science Journal》2018,89(10):1398-1405

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Molecular epidemiology of Shiga toxin‐producing O113:H21 isolates from cattle and meat          下载免费PDF全文

A. M. Sanso  A. V. Bustamante  A. Krüger  J. S. Cadona  R. Alfaro  M. E. Cáceres  D. Fernández  P. M. A. Lucchesi  N. L. Padola 《Zoonoses and public health》2018,65(5):569-577

The serotype O113:H21 is considered one of the relevant non‐O157 STEC serotypes associated with severe human infections. Due to the increased detection of O113 strains and their relationship with clinical cases, which emphasizes the importance of this serogroup as an emerging pathogen, our aim was to determine the characteristics of STEC O113:H21 strains circulating in bovine cattle and retail meat from Argentina. For this purpose, we determined the presence and combinations of various virulence genes (and their variants) related to adhesion and toxicity in a collection of 34 isolates. Their genetic relatedness using multiple‐locus variable‐number tandem repeat analysis (MLVA) was also studied. Subtyping of stx genes indicated that O113:H21 strains circulating in Argentina mainly present stx_2a alone or together with stx_2c or, less frequent, with stx_2d, all of which are subtypes associated with human disease. We found plasmid markers, such as saa, ehxA and subA, in a higher proportion than previous studies, and five variants of saa, two of which were novel ones. In relation to MLVA subtyping, we detected a limited diversity among the isolates considering that several loci were not discriminative and, that in some farms, the same clone seemed to remain circulating throughout the year. The O113:H21 strains studied harbour several toxin and adhesion genes (saa, espP, fimCD, ehaA, iha, hcpA, elfA, lpfO113, ecpA, subA, cdt‐V) and Stx subtypes associated with human disease. Results also highlighted that subtyping of stx and saa is useful to discriminate O113:H21 strains that share virulence genes. In conclusion, this study shows that a number of O113:H21 strains that occur in foods and bovines could be pathogenic for humans. This situation calls for further attention in the prevention and control of foodborne disease caused by these strains.  相似文献   

小麦新抗源——贵农21抗白粉病基因分析   总被引：3，自引：0，他引：3  

任明见  朱文华等 《种子》1999,(6):14-16

用4个具有不同毒力的小麦白粉菌生理小种,分别接种贵农21与5个抗感不同白粉菌小种的品种杂交的F1、F2和部分组合的BC1F1,群体的幼苗离体叶段,定单株编号和统计,初步鉴定出贵农21具有2对独立的显性抗病基因,其中1对只抗1和11号小种,与P38携带的抗1和11小种的基因相同;另1还对抗311和313号小种,与P38携带的另1对基因不同。  相似文献   

我国面向21世纪棉花纤维品质改良对策   总被引：28，自引：1，他引：27  

郭香墨  刘正德  罗云佳 《棉花学报》1999,(6)

棉花纤维品质与纺织品质量、市场竞争力和植棉业的发展密切相关。经过近２０ 年的改良, 我国２８～２９ｍ ｍ 长度的棉花品种综合纤维品质指标与国外相差不大, 但品种的纤维长度类型单一。随着纺织业设备更新和技术改造, 需要通过培育不同档次纤维的棉花品种、提高早熟性和抗病虫性多种途径进行改良, 也需要栽培技术的改进。纤维内在品质在流通领域的价值急需考虑。  相似文献   

谈迈向新世纪的中国园林设计     

刘晖 《江苏林业科技》1998,(Z1)

在简述中国传统园林辉煌成就的前提下,从服务对象、设计思想、构成园林的物质要素等方面比较古今园林设计的种种差异,提出在现代园林设计中应该更新设计观念,树立生态意识,改善物质技术手段,以超越辉煌的“过去”,谱写２１世纪园林设计的新篇章。  相似文献   

GS豫麦21号的丰产,稳产性及产量结构分析   总被引：6，自引：0，他引：6  

牛子敬  赵虹 《种子》1998,(2):24-26

利用黄淮南片水地小麦良种区试和河南省区试、生产示范资料,对GS豫麦21号的丰产性、稳产性和产量结构进行分析。结呆在明：GS豫麦21号具有丰产稳产性好、适应性广、产量构成三要素协调等优良特性,在产量构成三要素中,千粒重对产量的直接作用最大,穗粒数次之,有效穗数的直接作用最小,因此在高产栽培上应在兼顾667m2成穗（40万上下）的基础上,主攻千粒重和穗粒数的增加。  相似文献