排序方式： 共有217条查询结果，搜索用时 15 毫秒
81.
Jakub Kuczyński Tomasz Twardowski Jerzy Nawracała Joanna Gracz-Bernaciak Agata Tyczewska 《Journal of Agronomy and Crop Science》2020,206(6):759-772
Chilling stress is a major factor limiting the yield of soya bean [Glycine max (L.) Merr.] on a global scale. However, the regulatory network that controls the chilling response of soya bean remains unclear. In the present study, phenotyping and quantitative analyses of miRNAs in soya bean under chilling stress were carried out to determine the impact of environmental constraints on soya bean productivity. Measurements done during soya bean growth in chilling along with the results of field trials indicated that the cultivars Augusta and Fiskeby V responded differently to low temperatures. Although chilling affected the reproductive development of both cultivars, the final seed output remained unchanged. The differential expression of miR169, miR319, miR397 and miR398 under cold stress was detected using ddPCR. Upon chilling in the reproductive stage, we found that these miRNAs had contrasting expression profiles in Augusta and Fiskeby V. A set of candidate target genes was predicted based on degradome sequencing data. A negative correlation was found between the expression of miR169, miR319 and miR398 and their targets in the roots of both cultivars. Our work elucidates the impact of chilling stress on the productivity of two soya bean cultivars and reveals the importance of miRNA involvement in the low temperature response. 相似文献
82.
利用统计学方法分析了microRNA二级结构对Dicer酶剪切位点的影响,对于microRNA成熟体预测方法有一定参考价值。 相似文献
83.
microRNA对细胞的增殖和分化有着重要的调节作用,从大白猪(Susscrofa)基因组DNA上扩增microRNA-378-1前体序列,经XhoⅠ酶切后回收相应片段,连接到pEGP-miR载体中,构建重组载体pEGP-miR-378-1,转染猪胎儿成纤维细胞系(PEF),通过荧光观察转染效率及报告基因的表达效率,利用实时荧光定量PCR(QRT-PCR)测定细胞内microRNA-378-1的表达活性。结果表明,成功地构建了重组载体pEGP-miR-378-1;荧光观察转染后的细胞,显示有较高的转染效率,报告基因有较高的表达效率;QRT-PCR结果显示,实验组和对照组microRNA-378-1表达显著提高,实验组和对照组细胞间差异极显著(P<0.01);为制备转基因动物研究microRNA-378-1功能提供技术平台。 相似文献
84.
microRNA(miRNA)是一类内源性非编码小RNA,在植物整个生长发育过程中都起着重要作用,是基因表达的重要调控因子,而microRNA172(miR172)是众多miRNA家族中的重要的一员。为了更直观的认识miR172在植物中所发挥的作用,笔者归纳和总结了miR172在调控植物生长发育过程和响应逆境的研究进展,具体分析了miR172在植物营养生长阶段转变、开花、花器官发育、节间长度、植物商品器官发育以及逆境胁迫响应等诸多过程中发挥的作用。笔者认为miR172在植物中仍有很多未知的功能尚未发掘,因此未来深入挖掘和研究miR172在植物生长过程中更多的作用是一个重要的研究方向,在此基础上深入解析miR172在此过程中的功能、分子调控机制,为人为利用miR172调控植物生长发育奠定理论基础。 相似文献
85.
MicroRNAs(MiRNAs)是一种长度为20 nt左右的内源调控型非编码RNA,主要参与基因的转录后调控,在真核生物的生长发育、细胞分化和免疫防御等过程中发挥重要作用。海胆和海参属棘皮类动物,是高等的海洋无脊椎动物,它们不仅是研究无脊椎动物向脊椎动物进化的重要模式生物,其中的一些种类还是重要的渔业资源,具有较高的经济价值。近年来,探明各类miRNAs在海胆和海参生长发育及生理代谢过程中的调控功能及调控机制已逐渐成为海胆和海参研究领域的热点。本研究中综述了近年来海胆和海参中miRNAs的研究成果和相关进展,以期进一步丰富和完善海胆和海参中miRNAs的基础资料,为系统了解和掌握海胆和海参中miRNAs的序列特点、生物学功能及其参与调控重要生理过程的分子机制提供参考资料。 相似文献
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为了寻找线虫生殖相关基因,为开发线虫生殖抑制药剂提供理论基础,通过分析水稻干尖线虫转录组文库高通量测序数据,对Unigene进行KEGG路径分析,筛选出一个孕酮介导的卵母细胞形成代谢路径(progesterone-mediated oocyte maturation),并筛选出该路径的主要调控基因——锌指蛋白基因。通过同源序列比对发现水稻干尖线虫锌指蛋白氨基酸序列中含有核酸结合保守结构域NLS,进一步通过三维结构分析及分子对接验证该蛋白可与DNA分子结合。结果表明,水稻干尖线虫锌指蛋白可与其调控基因的启动子结合从而完成对孕酮介导的卵母细胞形成代谢路径的调控。通过本研究得出最终结论,水稻干尖线虫锌指蛋白具有生物防治价值,值得深入研究。 相似文献
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89.
Wangsheng Zhao Tajmal Hussain Solangi Yitao Wu Xiankang Yang Chuanfei Xu Hongmei Wang Xuxin Zheng Xin Cai Jiangjiang Zhu 《Reproduction in domestic animals》2021,56(4):555-576
The epididymis is the site of post-testicular sperm maturation, which constitutes the acquisition of sperm motility and the ability to recognize and fertilize oocytes. The role of miRNA in male reproductive system, including the control of different steps leading to proper fertilization such as gametogenesis, sperm maturation and maintenance of male fertility where the deletion of Dicer in mouse germ cells led to infertility, has been demonstrated. The identification of miRNA expression in a region-specific manner will therefore provide valuable insight into the functional differences between the regions of the epididymis. In this study, we employed RNA-seq technology to explore the expression pattern of miRNAs and establish some miRNAs of significant interest with regard to epididymal sperm maturation in the CY epididymis. We identified a total of 431 DE known miRNAs; 119, 185 and 127 DE miRNAs were detected for caput versus corpus, corpus versus cauda and caput versus cauda region pairs, respectively. Our results demonstrate region-specific miRNA expression in the CY epididymis. The GO and KEGG enrichment for the predicted target genes indicated the functional values of miRNAs. Furthermore, we observed that the expression of miR-200a was downregulated in the caput, compared with cauda. Since the family of miR-200 has previously been suggested to contribute to the distinct physiological function of sperm maturation in epididymis of adult rat, we speculate that the downregulation of miR-200a in CY caput epididymis may play an important role of sperm maturation in the epididymis of CY. Therefore, our findings may not only increase our understanding of the molecular mechanisms regulated by the miRNA functions in region-specific miRNA expression in the CY epididymis, it could provide a valuable information to understand the mechanism of male infertility of CY. 相似文献
90.
首先选用60日龄80只生长性能相近的SVF小鼠,随机分为2组,通过腹腔注射方式,对照组注射生理盐水,试验组注射胰岛素,试验期为0.5,1.0,2.0 h以及14 d;14 d后试验则是每48 h注射1次,测定肝脏中有关的microRNA的表达量。接着选用60日龄生长性能相近的10只miR-130a敲除鼠和10只野生型小鼠,随机分为2组,进行胰岛素耐受试验和葡萄糖耐受试验。结果显示,与对照组相比,在注射胰岛素0.5,1.0 h以及连续注射14 d时,miR-146a-5p、miR-143a-3p、miR-125b-2表达显著下调(P<0.05),而miR-130a-3p表达显著上调(P<0.05),在2 h时检测的microRNA均不显著,且在1.0 h时下调与上调的趋势更显著;同时在1.0 h时检测糖类代谢以及脂类代谢相关基因的表达,发现GSK、HK、CD36、FATP1基因表达显著下调,GS基因表达显著上调(P<0.05),但是到了14 d,CD36基因的表达则不存在差异;在敲除miR-130a-3p小鼠中出现胰岛素抵抗与胰岛素耐受的情况。结果表明,miR-146a-5p、miR-143a-3p、miR-125b-2、miR-130a-3p均参与了胰岛素调节的过程,且miR-130a敲除小鼠的胰岛素敏感性降低,为进一步了解胰岛素调节的作用提供有效的试验数据。 相似文献