Genome-Wide Selection of MAPKKK Gene Family in Gossypium raimondii and the Expression of Orthologs in Gossypium hirsutum     

Xu Haobiao  Li Libei  Zhang Chi  Feng Zhen  Yu Shuxun 《棉花学报》2019,31(6):459-473

[Objective] The MAPKKK gene family plays an important regulating role in response to multiple abiotic stresses and the development of plant. This study aims to identify MAPKKK genes of Gossypium raimondii and analyze their functions. [Method] In this study, based on G. raimondii genome database and bioinformatics method, G. raimondii MAPKKK family genes were identified and analyzed. Using the MEGA5, GSDS and Mapchart program, the phylogenetic tree, gene structure and chromosomes location analyses were accomplished. Based on the existing microarray data in cotton and comparative profiles of these MAPKKK genes, different expression of them in multiple abiotic stresses and the expression at different cotton fiber developmental stages were analyzed. [Result] A sum of 114 MAPKKK genes were identified systematically in G. raimondii and classified into 3 subfamilies (Raf, ZIK and MEKK) according to the gene stucture and phylogenetic tree analyses. They were distributed on all the 13 chromosomes of G. raimondii, and segmental duplication and tandem duplication events may have occurred. Compared with the recently released 78 genes of G. raimondii MAPKKK family genes, 47 sequences are exactly the same ones. [Conclusion] The results are helpful to understand the evolution and function of MAPKKK gene family. Our results provide a foundation for future functional characterizations of MAPKKK genes in cotton and probably other Gossypium plants.  相似文献   

金黄色葡萄球菌px-1 DNA芯片的制备及检测     

温尚昆 《山东农业大学学报(自然科学版)》2005,36(4):521-523

提取金黄色葡萄球菌px—1总DNA经酶切后,与载体质粒Puc18链接,并转化E.coli DH5α,从而构成金黄色葡萄球菌px—1的基因文库。用PCR方法扩增基因文库,所得PER产物点于芯片上,从而制各px—1DNA芯片。分别提取不同盐浓度培养的px—1的总RNA,把不同盐浓度培养的px—1的总RNA反转录为DNA,用Cy3,CY5分别标记反转录的DNA。通过杂交检测芯片,并获得杂交图像。  相似文献   

菲律宾蛤仔过敏原可视化抗体微阵列玻片的检测方法   总被引：3，自引：1，他引：2  

李小燕  李振兴  林洪  SAMEE Haider  张轶群 《水产学报》2010,34(3):422-427

采用辣根过氧化物酶(HRP)与3,3′,5,5′—四甲基联苯胺(TMB)—H2O2作为信号示踪系统,建立了一种可视化抗体微阵列检测菲律宾蛤仔过敏原的方法。对孵育时间、抗体浓度等免疫条件进行了优化。采用改良双抗体夹心法,将菲律宾蛤仔过敏原的兔多克隆抗体固定于琼脂糖三维芯片上,依次加入待检样品、菲律宾蛤仔鼠多克隆抗体和HRP-羊抗鼠IgG,孵育后加TMB显色,肉眼观察后,用平板扫描仪获取扫描图象,采用GenePixPro6.0软件分析灰度值。试验结果表明,该方法最低可检出10ng/mL的菲律宾蛤仔过敏原,片内平均变异系数(CV)为5.99%,片间为10.3%;香肠及蟹棒中3个不同浓度的加标回收率为73.54%～95.44%;4℃存放4个月内抗体微阵列玻片活性保持稳定。该方法不需要大型精密仪器,结果直观可见,可以发展为对多种过敏原进行同时检测,具有良好的实用和推广价值。  相似文献   

Development of a DNA microarray for species identification of quarantine aphids     

Won Sun Lee  Hwalran Choi  JinSeok Kang  Ji‐Hoon Kim  Si Hyeock Lee  Seunghwan Lee  Seung Yong Hwang 《Pest management science》2013,69(12):1399-1406

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薯蓣皂苷元对人胃低分化粘液腺癌MGC-803细胞基因表达的影响     

何忠梅  陈红岩  蔡恩博  李成恩  王月亮  郜玉钢 《人参研究》2014,(1):44-46

目的利用基因芯片研究薯蓣皂苷元对人胃低分化粘液腺癌MGC-803细胞基因表达的影响。方法将人胃低分化粘液腺癌MGC-803细胞培养12小时后,分为对照组和薯蓣皂苷元(30μg·mL-1)干预24小时组。干预结束后,提取mRNA,干预组和对照组逆转录分别用Cy5和Cy3标记,成为两组cDNA探针。cDNA探针与基因芯片杂交,经扫描仪扫描,用软件分析Cy5和Cy3两种荧光信号的强度和比值。结果芯片结果显示筛选出差异表达基因共158条,其中101条表达下降,57条表达增加。结论通过基因芯片技术,发现多个基因的表达水平可受薯蓣皂苷元用药的影响,通过对细胞基因表达的深入研究,对进一步理解薯蓣皂苷元抗肿瘤的分子机制可能有帮助。  相似文献   

Development of a novel equine whole transcript oligonucleotide GeneChip microarray and its use in gene expression profiling of normal articular‐epiphyseal cartilage     

K. E. GLÄSER  Q. SUN  M. T. WELLS  A. J. NIXON 《Equine veterinary journal》2009,41(7):663-670

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Toxicogenomic biomarkers for liver toxicity     

Kiyosawa N  Ando Y  Manabe S  Yamoto T 《Journal of toxicologic pathology》2009,22(1):35-52

Toxicogenomics (TGx) is a widely used technique in the preclinical stage of drug development to investigate the molecular mechanisms of toxicity. A number of candidate TGx biomarkers have now been identified and are utilized for both assessing and predicting toxicities. Further accumulation of novel TGx biomarkers will lead to more efficient, appropriate and cost effective drug risk assessment, reinforcing the paradigm of the conventional toxicology system with a more profound understanding of the molecular mechanisms of drug-induced toxicity. In this paper, we overview some practical strategies as well as obstacles for identifying and utilizing TGx biomarkers based on microarray analysis. Since clinical hepatotoxicity is one of the major causes of drug development attrition, the liver has been the best documented target organ for TGx studies to date, and we therefore focused on information from liver TGx studies. In this review, we summarize the current resources in the literature in regard to TGx studies of the liver, from which toxicologists could extract potential TGx biomarker gene sets for better hepatotoxicity risk assessment.  相似文献   

Difference in virulence between Staphylococcus aureus isolates causing gangrenous mastitis versus subclinical mastitis in a dairy sheep flock     

Eric Vautor  Joshua Cockfield  Caroline Le Marechal  Yves Le Loir  Marl��ne Chevalier  D. Ashley Robinson  Richard Thiery    Jodi Lindsay 《Veterinary research》2009,40(6)

Staphylococcus aureus mastitis in dairy sheep ranges from subclinical mastitis to lethal gangrenous mastitis. Neither the S. aureus virulence factors nor the host-factors or the epidemiological events contributing to the different outcomes are known. In a field study in a dairy sheep farm over 21 months, 16 natural isolates of S. aureus were collected from six subclinical mastitis cases, one lethal gangrenous mastitis case, nasal carriage from eight ewes and one isolate from ambient air in the milking room. A genomic comparison of two strains, one responsible for subclinical mastitis and one for lethal gangrenous mastitis, was performed using multi-strain DNA microarrays. Multiple typing techniques (pulsed-field-gel-electrophoresis, multiple-locus variable-number, single-nucleotide polymorphisms, randomly amplified polymorphic DNA, spa typing and sas typing) were used to characterise the remaining isolates and to follow the persistence of the gangrenous isolate in ewes’ nares. Our results showed that the two strains were genetically closely related and they shared 3 615 identical predicted open reading frames. However, the gangrenous mastitis isolate carried variant versions of several genes (sdrD, clfA-B, sasA, sasB, sasD, sasI and splE) and was missing fibrinogen binding protein B (fnbB) and a prophage. The typing results showed that this gangrenous strain emerged after the initial subclinical mastitis screening, but then persisted in the flock in the nares of four ewes. Although we cannot dismiss the role of host susceptibility in the clinical events in this flock, our data support the hypothesis that S. aureus populations had evolved in the sheep flock and that S. aureus genetic variations could have contributed to enhanced virulence.  相似文献   

Changes in hepatic gene expression in dogs with experimentally induced nutritional iron deficiency     

Michael M. Fry  Claudia A. Kirk  Jason L. Liggett  Gregory B. Daniel  Seung Joon Baek  Julia S. Gouffon  Pradeep M. Chimakurthy  Bhanu Rekapalli 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(1):13-19

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麝香草酚对酿酒酵母表达谱影响的研究   总被引：1，自引：0，他引：1  

梁晓英  郭娜  金晶  邓旭明  于录 《青岛农业大学学报(自然科学版)》2009,26(3)

麝香草酚是从百里香和陈皮中的提取的精油,具有抗病原真菌的活性。本实验应用基因芯片技术从全基因组水平考察了麝香草酚对酿酒酵母表达谱的影响,并用聚类软件T-profiler,分析了基因芯片数据。结果表明,酵母细胞中与磷脂合成、铁吸收等有关的基因的表达受到麝香草酚的影响。  相似文献