大兴安岭湖通河野生五味子药用资源保护区自然生态质量评价分析     

唐志强  孙希军 《林业科技情报》2012,(1):19-19,21

从植被类型多样性、物种多样性、生态系统的稳定性和脆弱性、面积适宜性几个方面对大兴安岭湖通河五味子药用资源保护区的自然生态质量进行了评价和分析。  相似文献   

Response of rice to sulphur application under upland conditions     

《Communications in Soil Science and Plant Analysis》2012,43(6):585-598

Abstract

A greenhouse experiment was carried out to determine the S response and the plant S content of rice (Oryza sativa L.) cultivars OS‐6 and IR‐20 grown in sandy Apomu soil series under upland conditions.

Sulphur application increased growth and dry matter yield. At low S rates OS‐6 gave higher grain yield than IR‐20. With high S rates, OS‐6 responded more in straw production. The leaf S content was highest during early growth and decreased with plant age. At flower emergence, the blade of the Y‐leaf appeared to be a suitable index for measuring the S status of the plant. The critical S level was estimated at 0.15%. The critical S levels in the grain and straw at harvest were estimated respectively at 0.12%, and 0.10%. The N/S ratios for the grain and straw at harvest appeared not to be a useful index for determining the critical S status of the plant.  相似文献   

谈中小桥采用后张法16m预应力空心板的施工问题     

李志宏  杨海军 《森林工程》1999,15(4):55-55,29

本文阐述了中小桥上部结构采用后张法１６ｍ预应力空心板的施工时应注意的问题，如预应力空心板的预制中底模的施工；侧模、蕊模；砼的要求；浇筑空心板的具体步骤。  相似文献   

水稻广亲和温敏不育系株1 S的选育及应用   总被引：48，自引：16，他引：32  

杨远柱  唐平徕  杨文才 《杂交水稻》2000,15(2):6-7,9

株１Ｓ是用遗传 远的不同生态类型材料杂交充 灿型广亲和温敏不育系。育性转换临界温度２３℃左右，在湖南稳定不育期９０ｄ以上，开花习性好，异交结实率４０％以上。海南冬敏少本地低温水深灌繁殖自交结实率在３０％以上，具有广亲和性，一般配合力和特殊配合力均较强，用其选配的早晚稻组合已有４个参加省区试。具有广阔的应用前景。  相似文献   

Accessions of Australian Nicotiana species suitable as indicator hosts in the diagnosis of plant virus diseases   总被引：2，自引：0，他引：2  

P. Van Dijk  F. A. Van Der Meer  P. G. M. Piron 《European journal of plant pathology / European Foundation for Plant Pathology》1987,93(2):73-85

When screening the genusNicotiana for sensitive and differential hosts for a group of mechanically transmissible plant viruses with narrow host ranges, development of systemic symptoms was alsmost exclusively observed in species of three closely related sections of the subgenusPetunioides. These species wereN. miersii (sectionAcuminatae),N. bigelovii andN. clevelandii (Bigelovianae) andN. benthamiana, N. cavicola, N. ingulba, N. occidentalis, N. rosulata andN. rotundifolia (Suaveolentes). Except forN. benthamiana andN. clevelandii, which are already known for their large virus ranges, they are new experimental hosts that appeared very useful for detection of viruses and for differentiation of viruses that closely resemble each other in host range. Accessions of the same species often varied largely in local and systemic viral response. EspeciallyN. benthamiana-9,N. miersii-33 andN. occidentalis-37B (code numbers given by Tobacco Research Laboratory, Oxford, N.C., USA) are recommended for routine inoculation tests. The sensitiveSuaveolentes species mentioned are native to the arid parts of Australia. Collections of these species deserve attention in studies on virus diseases of unknown etiology where experimental hosts are lacking.Samenvatting In het genusNicotiana werden vatbare, gevoelige en differentiële toetsplanten opgespoord door toetsing van vooral de collectie van het Tobacco Research Laboratory (Oxford, N.C., VS) met een aantal virussen waarvoor weinig of geen bruikbare toetsplanten bekend waren. Systemische reacties werden bijna uitsluitend in soorten van drie nauw verwante secties van het subgenusPetunioides waargenomen. Deze soorten warenN. miersii (sectieAcuminatae),N. bigelovii enN. clevelandii (Bigelovianae) enN. benthamiana, N. cavicola, N. ingulba, N. occidentalis, N. rosulata enN. rotundifolia (Suaveolentes). Behalve voor virusvermeerdering bleken deze soorten ook zeer geschikt te zijn voor differentiatie van virussen of virusstammen met een bijna gelijke waardplantenreeks.Collectienummers van één en dezelfde soort konden sterk in lokale en systemische reactie verschillen. VooralN. benthamiana-9,N. miersii-33 enN. occidentalis-37B kunnen voor routine-inoculaties worden aanbevolen. Met uitzondering vanN. benthamiana enN. clevelandii zijn de genoemde soorten of genotypen nieuwe experimentele waardplanten voor de virologie. De soorten die van de sectieSuaveolentes worden genoemd, zijn inheems in de woestijnachtige gebieden van Australië. Verzamelingen daarvan verdienen nadere aandacht bij de diagnostiek van virusziekten waarvoor nog geen experimentele waarplanten voorhanden zijn.  相似文献   

模拟降水量对毛乌素沙柳幼苗蒸发蒸腾的潜在影响   总被引：4，自引：0，他引：4  

肖春旺 《草地学报》2001,9(2):121-127

选择毛乌素沙地优势灌木沙柳为对象,研究157.5、315、472.5和630 mm 4种人控年降水量,对沙柳幼苗蒸发蒸腾作用的影响.结果表明,沙柳蒸发蒸腾量随着降水量的增加而逐渐增大,从157.5 mm增至630 mm,日蒸散量分别为1.63、3.11、4.37和5.66 mm,其中沙地蒸发量占主要部分.少量降水时,幼苗蒸腾速率明显呈现"午睡”现象,而充足降水则能消除"午睡”现象.相关分析结果表明,环境因子,如沙地含水率、太阳有效辐射、大气温度和饱和水汽压差,以及植物因素,如叶面积指数、叶片温度和气孔导度等,均能显著影响沙柳的蒸腾作用.  相似文献   

福建马铃薯S病毒的分子鉴定及发生情况   总被引：2，自引：0，他引：2       下载免费PDF全文

吴兴泉  陈士华  魏广彪  吴祖建  谢联辉 《植物保护学报》2005,32(2):133-137

为明确福建省马铃薯S病毒(PVS)的发生与分布情况,对福建省马铃薯主要种植区的PVS进行了鉴定和普查.在利用电镜技术和传统生物学方法鉴定的基础上,克隆了PVS外壳蛋白(cp)基因,依据PVS外壳蛋白氨基酸序列建立了PVS不同分离物的系统进化树.研究表明,利用PVS 外壳蛋白氨基酸序列分析可准确鉴定PVS,同时可分析不同分离物间的分子差异.利用病毒特异性引物和DIG标记的PVS cp基因为探针,分别利用RT-PCR技术和核酸斑点杂交技术(NASH)对PVS进行了检测,并对检测技术进行了改进.调查结果表明,PVS在福建省广泛分布,发病率最高可达80%以上,当地农家自留种可能是田间PVS的主要来源.  相似文献   

桃红叶植原体检测及鉴定   总被引：2，自引：0，他引：2  

张华明  蔡红  陈海如  葛晓琴 《植物检疫》2005,19(1):7-11

对表现红叶的桃植株进行植原体16SrRNA基因PCR扩增,得到1.2kb的特异片段.将此片段与pGEM T Easy载体连接并转化到大肠杆茵DH5α感受态细胞中.通过酶切、PCR鉴定,对筛选得到的重组阳性克隆进行序列测定及同源性比较分析,确定该株系属于翠菊黄化植原体组(Aster yellows group,16SrI).在国内首次报道了翠菊黄化组中的植原体侵染桃树.  相似文献   

中国禽(番鸭)呼肠孤病毒分离株S1基因全序列分析   总被引：14，自引：0，他引：14  

王劭  邓国华  吴异健  吴宝成  陈化兰 《中国预防兽医学报》2005,27(2):105-111

采用RT-PCR技术,分别以DRV-YH、DRV-YJL两株中国禽(番鸭)呼肠孤病毒RNA为模板,扩增了S1全基因的cDNA片段.将S1 cDNA克隆到T载体后进行序列测定,测序结果表明所扩增的cDNA片段长1643个核苷酸,包含了完整的S1基因的三个开放阅读框架(ORF1,ORF2和ORF3)和基因两端的非编码区.核苷酸序列比较分析结果表明:DRV-YH与ARV-S1133,176分别有6个,10个核苷酸的差异,DRV-YJL与ARV-S1133,176分别有8个,12个核苷酸的差异,DRV-YH与DRV-YJL有4个核苷酸的差异.  相似文献   

中国禽(番鸭)呼肠孤病毒YB株S4基因序列分析   总被引：5，自引：0，他引：5  

王劭  邓国华  吴异健  吴宝成  陈化兰 《中国预防兽医学报》2005,27(6):445-449

应用非免疫番鸭胚增殖中国禽(番鸭)呼肠孤病毒YB分离株,用LSTRIAZOL提取病毒RNA,反转录-聚合酶链反应(RT-PCR)扩增中国禽(番鸭)呼肠孤病毒YB分离株S4基因节段cDNA.将S4cDNA克隆到PMDl8-T载体上,并进行了鉴定和核苷酸序列测定.序列分析结果,克隆的S4cDNA共1 124bp,包括非编码区和完整的阅读框架.分子进化系统分析表明该毒株与禽呼肠孤病毒(鸡呼肠孤病毒)的亲缘关系较远,DRV-YB与DRV-89330同缘率为93.3%.  相似文献