鹿茸干细胞体外培养技术的研究     

孙红梅  邢秀梅  丛波  李春义  杨福合 《经济动物学报》2007,11(1):7-9,14

利用消化酶消化法,用DMEM培养基对鹿茸干细胞进行了体外培养,分别从原代培养、传代、冻存、以及复苏等几个环节进行了试验。摸索鹿茸干细胞合适的体外培养条件,为鹿茸的各项相关研究打基础。结果表明,用透明质酸酶和胶原酶对组织样进行消化,然后用10%DMEM培养基进行培养细胞生长效果较好。适宜培养条件是37℃、5%CO2最大饱和湿度。  相似文献   

Effects of Calcium Lactate on the Development of Chicken Embryos in a Shell-less Culture System up to Day Seventeen of Incubation     

Katsuya Obara  Chizuka Obara  Mitsuru Naito  Ikki Mitsui  Yumi Une  Atsushi Asano  Atsushi Tajima 《The Journal of Poultry Science》2022,59(2):168

This study examined the effects of calcium lactate on the development of chicken embryos in a shell-less culture system (cSLCS) up to the seventeenth day of incubation. In the presence of calcium lactate, a significant reduction in embryo viability was observed during the first week of incubation in cSLCS. On day 17 of embryo development, no significant difference was observed in the blood plasma calcium concentration or tibia bone density between cSLCS and intact control embryos, whereas the tibia length was significantly shorter in cSLCS embryos than in the intact control. These results suggest that calcium lactate supplementation in cSLCS supports bone formation in developing chicken embryos, but has adverse effects on the viability of embryos, particularly during the first week of embryo development.  相似文献   

奶牛乳腺上皮细胞三维培养的形态观察     

王秀美  考桂兰  高爱武  侯先志  高民 《畜牧与饲料科学》2011,(9)

利用原代培养后纯化的奶牛乳腺上皮细胞,以Matrigel为基质胶原,进行奶牛乳腺上皮细胞的三维培养,细胞接种浓度分别为1×104、1×105和1×106个细胞/mL。经过16d培养后,用DAPI对细胞染色,利用荧光显微镜观察细胞生长状况。结果表明,当细胞接种浓度为1×104～1×105个细胞/mL时,细胞形成了团块状结构,出现了类似腔状的腺泡形态。  相似文献   

中国传统木结构在继承和创新发展中的问题分析     

许建华  杨会峰  陆伟东 《木材工业》2011,25(5)

回顾总结我国传统木结构建筑的发展历史,并对我国传统木结构建筑及其文化的传承、推广进行深入分析;针对传统木结构发展瓶颈,提出我国传承和发展传统木结构的主要课题,指出现代科技在传统木结构发展中的重要作用,并从科研投入、人才培养、产学研合作及产业化等方面,对我国传统木结构的发展提出建议。  相似文献   

不同激素对碧云茶树丛生芽增殖培养的影响实验     

史俊  许建忠 《西部林业科学》2011,(4):70-72

为了获得碧云茶树的组培技术,加快其优良品种繁殖速度,2010～2011年进行了不同浓度的细胞分裂素6-BA和生长素NAA对碧云茶树丛生芽增殖培养的影响。结果表明,以MS为基本培养基,当6-BA为2.0 mg/L,NAA为0.2 mg/L时,对碧云茶树芽的诱导和增殖最有利,增殖率可达2.84;其中NAA差异不显著,6-BA差异显著。  相似文献   

老虎须组培实生苗快繁技术及其生根炼苗的移栽效果     

白艳荣  蒋亚莲 《贵州农业科学》2017,45(9)

为快速繁殖老虎须实生苗应用于实际,采用老虎须种子进行消毒,接种获得无菌实生苗,用叶片和叶柄诱导愈伤组织,进行增殖培养、生根培养和炼苗移栽。结果表明:2%次氯酸钠2 mL和0.1%氯化汞8mL组成的消毒液对老虎须种子消毒灭菌效果最好;温度对老虎须种子发芽率的影响不显著;最好的愈伤诱导培养基为MS+BA 0.2mg/L+NAA 0.1mg/L;MS+BA 3.0mg/L+NAA 0.1mg/L是最适宜的增殖培养基,MS+BA 0.5mg/L+IBA 0.5mg/L是最适宜的生根培养基。草炭∶珍珠岩为1∶1的基质移植练苗较好。  相似文献   

风箱树组织培养研究     

马妍  朱建峰  赵健如  王进茂 《河北林果研究》2012,27(2):147-151

以风箱树(Cephalanthus occidentalis L.)带腋芽的茎段为外植体,探讨不同植物激素(6-BA、IBA、NAA)对试管苗增殖以及植株再生的影响,建立了一套风箱树离体培养技术体系。试验结果表明:启动最适培养基为MS0;增殖最适培养基为MS+6-BA 1.5mg/L+IBA 0.2mg/L;生根最适培养基为1/4MS0;诱导率可达73.3%,增殖系数可达3.55,生根率89.47%,移栽成活率可达到100%。  相似文献   

“巨峰”葡萄热处理茎尖培养技术研究     

曹冬煦  周海峰  于生成  年玉欣 《北方园艺》2012,(9):116-118

以"巨峰"葡萄为试材,经热处理后取其茎尖进行离体培养,通过调整激素的种类和浓度,筛选出了适宜"巨峰"葡萄茎尖分化和生根的培养基;通过对不同移栽基质的研究,筛选出了移栽成活率较高的基质配方。结果表明:38℃热处理时葡萄苗发芽率最高,可达86.67%;以GS为基本培养基,附加BA 0.5mg/L与NAA 0.1mg/L,最适宜于"巨峰"葡萄茎尖分化成苗,分化率可达70.00%;生根培养基以GS+IBA 0.06mg/L的生根率最高。适宜"巨峰"葡萄茎尖组培苗移栽的基质为河沙1份+水苔1份。  相似文献   

TDZ对杏香兔耳风组培苗复壮的影响     

李峰卿  姚甲宝  曾满生  厉月桥  袁雅琪  周新华 《经济林研究》2015,(3)

为了了解TDZ对组培苗增殖的影响情况,探讨了TDZ在衰退组培苗复壮过程中的作用,以增殖衰退的杏香兔耳风组培苗为试验材料,就TDZ的不同添加方式(抽滤灭菌和高压灭菌)和不同浓度(0.0、0.2、0.4、0.6、0.8、1.0 mg/L)处理对组培苗增殖系数、有效芽及苗高生长的影响情况进行了试验。结果表明:当TDZ的浓度0.2 mg/L时,TDZ的添加方式对组培苗的增殖系数和有效芽数均有较大影响,抽滤灭菌后的增殖系数和有效芽数要明显高于高压灭菌的;不同浓度的TDZ对有效芽数和髙生长的影响均有极显著的差异性(P0.01),当TDZ浓度为0.8 mg/L时,苗质量总体达到最优,增殖系数为5.11,达到了预期的复壮效果;最优生根培养基配方为1/2MS+NAA 0.02 mg/L+IBA 0.1 mg/L。在杏香兔耳风组培苗复壮过程中,TDZ可作为一种有效的植物生长调节剂使用。  相似文献   

Intensive hatchery performance of Pacific white shrimp in the biofloc system under three different fertilization levels     

《Aquacultural Engineering》2016

Bacterial inorganic nitrogen control using carbon:nitrogen ratio (C:N) manipulation is a tool for aquaculture systems. The present study assessed the hatchery performance of Litopenaeus vannamei between the mysis 1 and postlarvae 5 stages in a zero-exchange biofloc system under different C:N fertilization levels (10:1, 12.5:1 and 15:1) with dextrose. Water quality, performance parameters and water microbiology were compared among treatments. The mean values of the evaluated water quality parameters were appropriate for this production stage. Fertilization with dextrose efficiently controlled ammonia levels, which did not reach the average concentrations considered toxic for the species. In the 10:1 C:N ratio treatment, the levels of ammonia started to increase early and showed significantly higher levels from the third to the last experimental day. There was no difference among groups in means of survival (>76%) and dry weight (0.26 mg) of L. vannamei production parameters and water quality were maintained without water exchange using a biofloc system supplemented with dextrose. Therefore, the use of biofloc systems without water exchange with dextrose as a carbon source in C:N ratios of 10:1, 12.5:1 and 15:1 results in both adequate production indexes and water quality during the misis 1 to post-larvae 5 hatchery phases of L. vannamei. However, the ratios of 12.5:1 and 15:1 keep lower levels of ammonia.  相似文献