用单抗研究T细胞与牛流行性白血病发生的关系     

潘耀谦  夏志平  高丰  冈田幸助 《动物医学进展》2001,22(4):75-78

本试验对10头患流行性白血病病牛的不同器官的肿瘤组织，用8种单克隆抗体（McAb),TH14B、BAQ44A、PIg45A、BIg715A、PIg501E、cAct105、MM1A、AHCC125染色进行了观察。结果：病牛经临床病理学和免疫琼扩试验而诊断为EBL，通过免疫组织化学检测，在10个病例中发现有9个病例的肿瘤组织对B细胞属性的McAb有很强的染色反应，证明其来源于B细胞。仅有1个病例对B细胞属性的McAb着色很淡，而对T细胞属性的McAb着色很深，呈强阳性反应，说明其来源可能与T细胞有关。  相似文献   

Bovine Cells Infected in Vivo with Theileria Annulata Express CD11b,the C3bi Complement Receptor     

Forsyth  L.M.G.  Jackson  L.A.  Wilkie  G.  Sanderson  A.  Brown  C.G.D.  Preston  P.M. 《Veterinary research communications》1997,21(4):249-263

Forsyth, L.M.G., Jackson, L.A., Wilkie, G., Sanderson, A., Brown, C.G.D. and Preston, P.M., 1997. Bovine cells infected in vivo with Theileria annulata express CD11b, the C3bi complement receptor. Veterinary Research Communications, 21 (4), 249-263Bovine cells from cattle infected with Theileria annulata were phenotyped with monoclonal antibodies recognizing bovine leukocyte antigens. Macroschizont-infected, transformed cell lines prepared from peripheral blood mononuclear cells of cattle, infected with sporozoites, were assessed by flow cytometry; parasitized cells in tissues from infected cattle were examined by immunocytochemical techniques. Co-expression of markers for different cell lineages by the cell lines precluded a definite conclusion as to their phenotypic origins. For, while the pattern of leukocyte antigens expressed by these in vivo-derived schizont-infected cells, which included CD11b, was indicative of a myeloid origin, the possibility that they were NK cells could not be excluded. The monoclonal antibody (MAb) IL-A15, which recognizes CD11b, reacted with a high proportion of parasitized cells in sections of tissues from infected cattle at all stages of acute disease. Mononuclear cells infected with parasites at all stages of differentiation, from macroschizont to microschizont, expressed CD11b. Such parasitized cells occurred throughout the lymphoid tissues, being found in the thymus, spleen and lymph nodes, particularly the prescapular node draining the site of infection, the hepatic, mesenteric and precrural nodes, as well as in the reticulo-endothelial tissue of the liver, kidney, lung, abomasum, adrenal and pituitary glands. These observations provided the first evidence for a myeloid origin for the parasitized T. annulata cells found in infected bovine tissues and blood and suggested a mechanism whereby schizonts could transfer from cell to cell during mechanical infection with schizont-infected cells.  相似文献   

Arabinogalactan proteins may facilitate the movement of pollen tubes from the stigma to the ovules in Actinidia deliciosa and Amaranthus hypochondriacus     

Sílvia Coimbra  Cristina Duarte 《Euphytica》2003,133(2):171-178

Sexual plant reproduction is a complex process that involves a series of interactions between the male gametophyte and the different cell types of the pistil. These interactions are believed to direct the pollen tube growth until its final target, the embryo sac. Arabinogalactan proteins are complex proteoglycans that are believed to be involved in these processes. The pistil is enriched in these highly glycosylated proteins and we provide results that show the selective presence of different AGP epitopes at the surface of the cells or in the ECM of the tissues that correspond exactly to the pollen tube growth pathway in Amaranthus hypochondriacus and Actinidia deliciosa. We also show that in Actinidia deliciosa, which is a dioecious plant with the male flowers having rudimentary ovaries where fertilization does not occur, there is no presence at all of the epitopes recognised by the monoclonal antibodies utilized in this study. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Genetics of resistance to five strains of turnip mosaic virus in Chinese cabbage   总被引：4，自引：0，他引：4  

S. K. Suh  S. K. Green  H. G. Park 《Euphytica》1995,81(1):71-77

Summary Inheritance of resistance to turnip mosaic virus (TuMV) strains C1, C2, C3, C4, and C5 in Chinese cabbage (Brassica campestris subsp. pekinensis) was evaluated using monoclonal antibodies. Crosses were made between a resistant line, 0–2, and four susceptible line. Seoul (SE),SSD31 (SS), Cheongbang (CH), and Yaki 1 ho (YA), to determine the inheritance of resistance of 0–2 in different genetic backgrounds. Resistance to TuMV was controlled by a single dominant gene or double dominant genes depending on the strain and cross. The resistance genes of 0–2 were modified by susceptible parents such that a single dominant gene was involved in the SS×0–2 combination, but double dominant genes in the SE×0–2 against TuMV-C3 or TuMV-C5. ELISA tests using inoculated and noninoculated leaves in the same plant suggested that the dominant resistance genes inhibit virus movement rather than virus multiplication.  相似文献   

单克隆抗体在植物病毒学中的应用     

韩英任艳玲  廖咏梅 《广西农业生物科学》2007,26(B06):142-144,149

单克隆抗体是由杂交瘤细胞分泌的针对单一抗原决定簇的抗体。单克隆抗体在植物病毒学中的应用非常广泛，包括对植物病毒的检测、种下分类级别划分、抗原决定簇鉴定、非结构蛋白功能分析、体内病毒含量测定及病毒纯化等。  相似文献   

抗鸭副粘病毒单克隆抗体的制备及其特性鉴定          下载免费PDF全文

程晓霞  朱小丽  陈少莺 《西北农林科技大学学报(自然科学版)》2011,39(2):54-58

【目的】制备抗鸭副粘病毒(DPMV)的单克隆抗体,为鸭副粘病毒病免疫学诊断方法的建立奠定基础。【方法】采用差速和蔗糖不连续密度梯度离心法提纯DPMV,免疫BALB/c小鼠,取脾细胞与骨髓瘤细胞株SP2/0融合,采用间接ELISA、间接免疫荧光试验(IFA)和血凝抑制(HI)试验等方法筛选阳性杂交瘤细胞株,并测定其生物学特性。【结果】筛选到7株能稳定分泌抗DPMV单克隆抗体的杂交瘤细胞株,分别命名为J28、F4、B3、E39、E41、A12和A30。这7株单克隆抗体都具有ELISA和1FA特性,其中J28和F4还具有HI和中和特性。免疫球蛋白亚类测定结果显示,J28和F4均为IgG1,其他5株均为IgM。相加ELISA试验结果显示,单抗B3,J28与F4,A30与E39/E41/A12分别识别3个不同的抗原位点。特异性测定结果显示,7株单克隆抗体与雏番鸭细小病毒(MPV)、鹅细小病毒(GPV)、鸭肝炎病毒(DHV)、番鸭呼肠孤病毒(MDRV)及正常细胞均无交叉反应。【结论】筛选出了7株具有ELISA、1FA等特性且分泌抗DPMV单克隆抗体的杂交瘤细胞株,并明确了各株单克隆抗体的特性,为鸭副粘病毒病的免疫治疗和临床诊断奠定了基础。  相似文献   

提高细胞融合率和杂交瘤细胞过量生长的方法          下载免费PDF全文

李健强  David T.Shen  William C.Davis 《西北农林科技大学学报(自然科学版)》1988,(2):17-22

B淋巴细胞促分裂素—鼠伤寒沙门氏菌(Salmonella typhimurium,STM)细胞壁水溶性蛋白提取物的应用,以及延迟使用HAT的选择性培养基等条件的改进,使细胞融合率提高到90％以上。分泌特异性抗体的杂交瘤细胞系阳性率提高到50％以上。并得到了不同类和亚类的单克隆抗体。本文也对某些影响杂交瘤细胞生长的因素和条件作了讨论。  相似文献   

水稻细菌性条斑病病原菌(Xanthomonas campestris pv.oryzicola)单克隆抗体的研究     

秦文胜  张永平  李德葆  李洸 《浙江大学学报(农业与生命科学版)》1990,(4)

应用淋巴细胞杂交瘤技术,融合经水稻细菌性条斑病病原菌(Xanthomonas campestris pv.oryzicola)免疫的BALB/C小鼠脾细胞和Sp2/O骨髓瘤细胞,经细条病菌典型菌株R17,R25和GX-1单独及三者混合包被进行ELISA检测筛选,建立了能稳定分泌特异性单克隆抗体的杂交瘤细胞株18G 9,19D8,21F2,21G3,25E6和26F9细胞株。体外连续传代培养三个月,液氮冻存四个月后复苏培养,分泌抗体稳定。六株杂交瘤细胞的腹水单克隆抗体ELISA效价在1:2000左右。各株细胞的培养上清液ELISA效价为1:8左右。18G9,19D8和25E6细胞株所分泌的抗体为小鼠IgM类,21F2和21G3分泌的抗体为小鼠IgG_3亚类,26F9分泌的抗体为小鼠IgG_(2b)亚类。根据与植物病原细菌4个属6个种的20个不同菌株的特异性反应,将六株单克隆抗体分为A,B,C三组,20个菌株分为3个血清型和1个菌株群。  相似文献   

沙门氏菌快速检测试剂盒的研制及其应用     

文其乙  焦新安  刘秀梵  张如宽 《扬州大学学报(农业与生命科学版)》1996,(2)

在建立直接ＥＬＩＳＡ方法基础上，首次研制出检测沙门氏菌的单抗快速检测试剂盒。对现场分离菌株进行的双盲考核试验共做２８０个菌株，两种方法的总符合率为９４．６％。以２０％的脱脂乳为冻干保护剂，经冻干后，其效价和物理性状最佳，试剂盒批内和批间重复试验的变异系数小于１０％，试剂盒的稳定性较好。采用本试剂盒检测了３０５９份人的粪样，检测出１６６份阳性样品，比市站多检出７２份阳性样品。在对６１９份肉样、６８８份鱼粉、６００份奶样、６２０份蛋样检测中，比常规国标方法多检出１００株沙门氏菌。因此，以直接ＥＬＩＳＡ为基础构建的快速检测试剂盒在沙门氏菌检验中，具有重要实用价值。  相似文献   

斑点金标免疫渗滤法诊断家畜血吸虫病的敏感性和特异性   总被引：1，自引：0，他引：1  

张雪娟  卢福庄  刘恩勇  方兰勇  阳爱国  何永强  付媛  项美华 《浙江农业学报》2006,18(6):453-457

研制成家畜血吸虫病斑点金标免疫渗滤法（DIGFA）诊断试剂盒可测出人工感染血吸虫14—42d的病牛、猪和兔的血清及血纸抗体，比病原体检查法提早28d查出病畜，达到早期诊断的要求；同时亦能检出仅感染1对血吸虫病牛抗体，证实DIGFA具有较高的敏感性。血吸虫与肝片吸虫、锥虫、蛔虫、囊虫和旋毛虫病之间未见交叉反应现象，说明本法具有较强的特异性。  相似文献