乙酰胆碱酯酶试剂包配方的选择     

王小瑜  王相友  孙霞  孙冬霞 《保鲜与加工》2007,7(6):38-41

以商品化乙酰胆碱酯酶试剂包为研究对象,比较了不同用量的乙酰胆碱酯酶、底物和显色剂下的抑制率,并在此基础上进行了正交试验,在保证一定的检测精度前提下最终确定了酶用量为原来的3/4,底物用量为原来的3/4,显色剂用量为原来的1/2,降低了检测成本。通过F检验证明,调整后配方与原配方之间无显著性差异。在此条件下,氧乐果、敌百虫、敌敌畏的检出下限大约分别为0.37mg/L、0.09mg/L、0.035mg/L,与原配方一致。  相似文献   

鸡排泄物中金霉素残留的测定方法研究   总被引：1，自引：0，他引：1  

李维  魏瑞成  王恬  王冉 《华北农学报》2008,23(Z1)

为研究建立抗生素药物残留的环境生态风险性评估提供方法,建立了鸡排泄物中金霉素残留高效液相色谱分析方法。鸡排泄物经复合提取液均质提取后,调节溶液pH后,离心过滤,以ZORBAX SB-C18色谱柱作为固定相,用0.01 mol/L乙二酸、甲醇、乙腈按77∶11.5∶11.5体积比混合溶液为流动相,在375 nm波长状态下进行HPLC检测。金霉素浓度在0.1~10.0μg/mL之间,其标准曲线呈线性相关,相关系数r2=0.999 8,在0.5~10.0 mg/kg浓度添加范围内,回收率71.46%~98.01%,日内变异系数0.89%~4.72%,日间变异系数1.94%~7.94%,检出限0.05 mg/kg。  相似文献   

我国食品中农药残留研究进展     

刘乃新 《中国园艺文摘》2009,25(3):134-135

随着人们安全健康意识的提高,食品中农药残留问题更加受到重视。为此,对样品预处理研究进展及农药残留快速检测技术研究进展进行综述,并简单介绍各种方法的优缺点。  相似文献   

cDNA cloning and complete primary structures of myosin heavy chains from brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso fast skeletal muscles     

Mohammed Anwar Hossain  Daisuke Ikeda  Akira Nomura  Hideto Fukushima  Shugo Watabe 《Fisheries Science》2008,74(4):921-934

ABSTRACT:   The complete cDNA sequences encoding predominant types of myosin heavy chain (MYH) in the fast skeletal muscle were determined for brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso , which are used as materials for preparing high-quality surimi-based products. The cDNA consisted of 5973 and 5987 bp, respectively, and both encompassed an open reading frame encoding a polypeptide of 1936 amino acid residues. Brushtooth and wanieso lizardfish MYH showed the amino acid sequence identity of 92–93% to white croaker MYH, which was higher than that of 90% to walleye pollack MYH. The putative binding sites for ATP, actin, and regulatory and essential light chains in the subfragment-1 region of brushtooth lizardfish MYH exhibited a high identity with white croaker counterparts as well as the sequences of subfragment-2 and light meromyosin. In contrast, phylogenetic tree, constructed by the neighbor-joining method based on mitochondrial 16S rRNA gene, revealed that the two lizardfish species formed a cluster with walleye pollack, which was paraphyletic with white croaker. Therefore, a good reputation for lizardfish and white croaker to have a high thermal-gel forming ability seemed to be reflected by MYH rather than biological similarity as revealed by the mitochondrial 16S rRNA gene.  相似文献   

香蕉中噻唑膦残留分析方法研究     

田海  冯玉洁  张艳玲  谢德芳  王明月  李琼  罗金辉 《热带作物学报》2017,38(5):945-949

建立香蕉中噻唑膦的超高效液相色谱串联质谱法(UPLC-MS/MS)残留测定方法。香蕉样品采用乙腈提取和PSA+C18分散固相萃取,采用UPLC-MS/MS多反应模式(MRM)进行分析测定。噻唑膦在添加水平0.01~1.0 mg/kg范围内,平均回收率为94.3%~107.5%,相对标准偏差为7.9%~11.0%。采用外标法定量,方法的检出限为0.001μg/m L,定量限为0.005 mg/kg。该方法简单、准确、重复性较好且节省溶剂,适用于香蕉中噻唑膦的残留检测。  相似文献   

Effect of xeroderma pigmentosum group D gene on proliferation of human umbilical arterial smooth muscle cells induced by Ox-LDL     

XIA Zi-rong  LI Qing  XIA Zhen  LI Ju-xiang  HONG Kui  WU Yan-qing  WU Qin-hua  CHENG Xiao-shu 《园艺学报》2017,33(12):2238-2244

AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G₀/G₁-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis.  相似文献   

Model construction of rat coronary artery smooth muscle cell endoplasmic reticulum stress induced by thapsigargin     

CHEN Xiao-yan  DENG Chun-yu  KUANG Su-juan  YANG Hui  RAO Fang  SHAN Zhi-xin  LIN Qiu-xiong  JIANG Li 《园艺学报》2017,33(1):128-132

AIM: To investigate the primary culture method for coronary artery smooth muscle cells (CASMCs), and to establish the endoplasmic reticulum stress (ERS) model in CASMCs of SD rats. METHODS: CASMCs were cultured by tissue explant method. The morphological characteristics were observed under optical microscope. The marker proteins of CASMCs, including α-SMA and SM-MHC, were identified by immunofluorescence technique. The protein expression levels of BiP and CHOP, the marker molecules of ERS, were determined by Western blot. RESULTS: The spindle-shaped CASMCs climbed out from the edge of coronary artery tissues after 6 d, and formed the typical "hill and valley" growth pattern of CASMCs at 9~10 d. The result of immunofluorescence technique showed that α-SMA and SM-MHC were positively expressed. The results of Western blot showed that the protein expression of BiP and CHOP in TG (1 and 2 μmol/L) treatment groups was increased compared with control group. Compared with control group, the protein expression of BiP and CHOP was significantly increased after 1 μmol/L TG treatment for 24 and 48 h. CONCLUSION: CASMCs can be successfully cultured by tissue explant method. ERS model of CASMCs was established by 1 μmol/L TG treatment for 24 h.  相似文献   

金银花中戊唑醇、苯醚甲环唑的残留分析     

王晓琳  刘玉霞  高素霞  杨帆  王飞  刘红彦 《中国农学通报》2017,33(19):143-147

为解决金银花种植过程中农药的使用问题,以金银花为试验对象,选用广谱杀菌剂戊唑醇和苯醚甲环唑,采用常量、倍量、二次和三次灌根处理,对最终的残留含量进行分析。头茬花各处理中苯醚甲环唑均未检出,而二茬花中戊唑醇的残留量为0.14~0.22 mg/kg,存在残留超标风险。建议使用10%苯醚甲环唑4000倍液灌根处理,防治金银花土传病害,灌根2~3次,安全间隔期15天。  相似文献   

tau‐Fluvalinate and other pesticide residues in honey bees before overwintering     

Tomas Erban  Marta Vaclavikova  Daniela Tomesova  Tatana Halesova  Jan Hubert 《Pest management science》2019,75(12):3245-3251

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我国农作物病虫害化学防控技术的社会成本分析   总被引：1，自引：0，他引：1  

杨普云  李春广 《中国植保导刊》2019,(4):21-25,69

农作物病虫害化学防控技术的负外部效应产生了环境和社会成本。评价化学防控技术的社会成本,具有十分重要的理论和现实意义。本文分析了农作物病虫害化学防控技术社会成本产生的原因与类别,指出农药中毒、接触农药导致的慢性疾病、农产品农药残留超标、农药包装废弃物回收处理、农药及农药残留管理行政事业费用、农药市场检测和监控、农药残留检测和监控是主要的社会成本类别。初步分析了各类别社会成本的经济影响额度,测算出我国使用化学农药防控农作物病虫害的所产生的社会成本至少在11.72～87.74元/kg(折百)。  相似文献