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11.
YANGXiu-qin LIUDi 《东北农业大学学报(英文版)》2005,12(1):65-67
In order to further study functions of the porcine myostatin gene, we analyzed the polymorphisms of porcine myostatin gene in promoter region among different breeds including Yorkshire, Landrace, Duroc, Junmu, Min pig and Sanjiang white pig by PCR-RFLPs. The allele T dominated in the imported lean-type pig breeds such as Yorkshire, Landrace and Duroc. No allele A was detected in Junmu and Sanjiang white pig, and the frequencies of three genotypes were about equal in Min pig. The result using X^2 analysis showed that the distribution of three genotypes was related to pig breeds. 相似文献
12.
Jianxiang XU Dengke PAN Jie ZHAO Jianwu WANG Xiaohong HE Yuehui MA Ning LI 《农业科学与工程前沿(英文版)》2015,2(1):90
Myostatin is a transforming growth factor-βfamily member that normally acts to limit skeletal muscle growth. Myostatin gene(MSTN) knockout(KO) mice show possible effects for the prevention or treatment of metabolic disorders such as obesity and type 2 diabetes.We applied chromatography and mass spectrometry based metabonomics to assess system-wide metabolic response of heterozygous MSTN KO(MSTN+/–) swine. Most of the metabolic data for MSTN+/–swine were similar to the data for wild type(WT) control swine. There were, however,metabolic changes related to fatty acid metabolism,glucose utilization, lipid metabolism, as well as BCAA catabolism caused by monoallelic MSTN depletion.The statistical analyses suggested that:(1) most metabolic changes were not significant in MSTN+/–swine compared to WT swine;(2) only a few metabolic properties were significantly different between KO and WT swine,especially for lipid metabolism. Significantly, these minor changes were most evident in female KO swine and suggested differences in gender sensitivity to myostatin. 相似文献
13.
Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3' coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. The homology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes. 相似文献
14.
Y. X. Yang J. Guo Z. Jin S. Y. Yoon J. Y. Choi M. H. Wang X. S. Piao B. W. Kim B. J. Chae 《Journal of animal physiology and animal nutrition》2009,93(6):732-743
To investigate the effects of lysine restriction and subsequent realimentation on growth performance, blood profiles and gene expression of leptin and myostatin, 128 weaned pigs [initial body weight (BW) 6.96 ± 1.07 kg, 26 ± 2 days of age] were randomly allotted to four treatments. The starter diets during the first 2 weeks (P1) contained 100%, 80%, 70% or 60% of recommended lysine levels ( National Research Council, 1998 ). Then, common grower 1 and 2 diets were offered for 2 weeks (P2 and P3) each. During P1, average daily gain (ADG) was linearly reduced (p < 0.05) with the increasing levels of lysine restriction. Growth rate was greater in pigs previously fed lysine‐restricted diets than well‐fed pigs although it did not reach a significant level during realimentation. However, the final BW and overall ADG were the lowest (p < 0.05) and F/G was poor in pigs fed 60% lysine diet. Relative visceral organ weights and composition of skeletal muscle were similar (p > 0.05) among the treatment. Blood triglyceride and glucose levels were increased (p < 0.05) during P1, while blood urine nitrogen, total protein and albumin levels were decreased (p < 0.05) during P2 with the reduction in dietary lysine levels. The abundance of myostatin mRNA in skeletal muscle and leptin mRNA in subcutaneous adipose tissue were lower (p < 0.05) in lysine‐restricted pigs than in pigs fed non‐restricted diets. In conclusion, 80% and 70% lysine restriction of starter diets resulted in inferior growth and compensatory growth effect was noted during realimentation, while 60% lysine restriction had a negative influence on growth performance. Moreover, the changes in myostatin and leptin mRNA abundance caused by nutritional manipulations may be involved in the regulation of protein and fat deposition in young pigs. 相似文献
15.
肌肉生长抑制素(myostatin)为转化生长因子TGF-beta超家族成员之一。随着研究的深入,发现鸡与哺乳动物myostatin基因的功能特性存在一定差异。本文主要综述了鸡myostatin基因在鸡骨骼肌生长发育和胚胎发生中的表达和功能等方面的研究进展。同时,对鸡作为肌肉相关研究领域模式动物的优势,肉鸡、蛋鸡间骨骼肌巨大生长差异与myostatin基因的可能关系也进行了简单讨论。 相似文献
16.
以与东北马鹿物种最近的牛(Bos taurus cattle,序列号为AB076403)的肌肉生长抑制素(myostatin,MSTN)基因序列为模版,根据该基因保守序列进行引物设计,扩增产物连接入pMD18-T载体,克隆出3个外显子片段.根据5′-GU-AG-3′规则和同源基因比对拼接出带有部分5′、3′末端的cDNA,并采用生物信息学技术对编码氨基酸序列进行蛋白质同源性比较和二级、三级结构等分析.结果表明:所获序列全长为1 128 bp,编码375个氨基酸;具有较明显的螺旋、片层和无规卷曲等二级结构;有1个较明显的跨膜区和1个疏水区,并发现明显的信号肽和糖基化位点.三级结构同源建模预测结果显示与Human Activin A的相似性为42%.结果说明东北马鹿和其他哺乳类生物的肌肉生长抑制素基因之间关系密切,并同属于TGF-β家族. 相似文献
17.
牛肌肉生长抑制素基因单核苷酸多态性分析 总被引：2，自引：1，他引：1
应用PCR-SSCP分析方法,对94头肉牛(公牛45头:西门塔尔34头,夏洛来11头;母牛49头:西门塔尔24头,夏洛来25头)的肌肉生长抑制素(MSTN)3个外显子进行了多态性分析.结果显示,第1外显子存在2种基因型,分别为AA型和AB型.经测序发现,第1外显子4 bp处存在C→G的碱基突变,导致编码的氨基酸由谷氨酰胺(Gln)→谷氨酸(Glu).统计结果表明,等位基因B的含量低,而且只在西门塔尔品种内含0.026 6.利用SPSS软件作最小二乘分析,结果表明,B等位基因与西门塔尔牛的成年体质量和犊牛出生体质量呈显著正相关. 相似文献
18.
Takayuki MIURA Yasuhiro KISHIOKA Jun-ichi WAKAMATSU Akihito HATTORI Takanori NISHIMURA 《Animal Science Journal》2010,81(1):102-107
Myostatin, a member of the TGF-β superfamily, is a negative regulator of skeletal muscle mass. We have recently demonstrated that decorin binds to myostatin in vitro , and that immobilized decorin within the collagen matrix prevents myostatin-mediated inhibition of myoblast proliferation. However, little is known about other ECM molecules that bind to myostatin and modulate its activity. Thus, in the present study, we investigated the interaction of several other ECM molecules with myostatin. We here show that fibromodulin, fibronectin and laminin bind to myostatin in the presence of Zn2+ with a dissociation constant ( KD ) of 10−10 ∼10−8 mol/L. Fibromodulin shows the highest affinity for myostatin among them. These results suggest that these ECM molecules may modulate myostatin activity like decorin does. 相似文献
19.
Masato MIYAKE Shinichiro HAYASHI Yoshikazu TAKETA Shunsuke IWASAKI Kouichi WATANABE Shyuich OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2010,81(2):223-229
Myostatin (MSTN) is a negative regulator during muscle differentiation, whereas insulin‐like growth factors (IGFs) are essential for muscle development. MSTN and IGFs act oppositely during myogenesis, but there is little information on the mutual relationship of MSTN and IGFs. The present study was conducted to examine whether MSTN affects IGF expression during early myogenesis in cattle. IGF‐1 mRNA was similarly expressed in M. longissimus thoracis of double‐muscled (DM) and normal (NM) Japanese shorthorn cattle. IGF‐2 mRNA expression was consistently higher in the normal and regenerating muscle of DM cattle than those of NM cattle. When myoblasts were isolated from regenerating M. longissimus thoracis, IGF‐2 mRNA expression showed a significant increase in differentiating DM derived myoblasts (DM‐myoblasts) as compared with differentiating NM derived myoblasts (NM‐myoblasts). An addition of recombinant mouse myostatin (rMSTN) to myoblast cultures attenuated IGF‐2 mRNA expression and decreased myotube formation, but did not effect IGF‐1 mRNA expression. An activin‐like kinase (ALK) inhibitor, SB431542, mediates MSTN action, suppressed the translocation of Smad2/3 into the nucleus in DM‐myoblasts, and restored the attenuated IGF‐2 mRNA expression and the decreased myotube formation induced by rMSTN in myoblast cultures. The findings indicate that MSTN may negatively regulate myoblast differentiation by suppressing IGF‐2 expression via ALK‐Smad signaling. 相似文献
20.
肌肉生长抑制素（myostatin,MSTN）又称生长分化因子8,是TGF-β超家族成员,也是肌肉发育的负调控因子。MSTN在家畜出生前、出生后都起到关键作用,并且决定家畜的整体肌肉含量,由于MSTN基因突变,导致牛和羊等许多物种表现出“双肌”现象。因此,一直以来,MSTN多态性及其对肉类产量的遗传效应研究是动物育种领域的热点。因此,本文首先介绍MSTN调控肌肉发育的机制通路,然后再探讨MSTN突变及其与相关表型的关系,以及改变MSTN表达对家养动物（牛、马、山羊、绵羊、猪、鸡和兔）的生理影响,最后,综述了MSTN基因多态在国内外畜禽中的研究进展。 相似文献