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101.
利用PCR法克隆了夏洛来肉牛抑肌素突变基因第二外显子,序列分析结果表明：突变基因与正常基因相比较,存在1个单碱基突变(C→A),从而使该基因表达产物——抑肌素的氨基酸序列发生变异,形成双肌性状。 相似文献
102.
Elangovan Prabu Nathan Felix Arumugam Uma Baboonsundaram Ahilan Cheryl Antony 《Aquaculture Nutrition》2020,26(5):1713-1723
A 60‐day indoor feeding trial was conducted to evaluate the effects of dietary tryptophan supplementation on growth performances, whole‐body chemical composition, expression of muscle growth‐related genes (MyoD, myogenin and myostatin), and haematological and biochemical responses of juvenile genetically improved farmed tilapia (GIFT). Five corn–soy‐based isonitrogenous and isoenergetic diets were formulated to contain graded levels of dietary tryptophan (2.6, 3.2, 3.7, 4.2 and 4.8 g/kg of diet). Each diet was randomly assigned to triplicate groups of 30 fish (5.3 ± 0.1 g) per experimental unit, which were fed thrice a day (9:00, 13:00 and 17:00 hr). Maximum growth performances and feed utilization were observed in fish fed tryptophan at 3.7 g/kg of diet. There was no significant (p > .05) effect on whole‐body composition and amino acid profile by dietary tryptophan supplementation. However, significant (p < .05) differences were observed in plasma metabolites and the mRNA expression of MyoD, myogenin and myostatin. Serum cortisol level was found significantly lowest in fish fed tryptophan at 3.7 g/kg of diet. Second‐order polynomial regression analysis of weight gain and nitrogen gain against dietary tryptophan levels indicated that the optimum dietary tryptophan requirement for maximum growth and feed utilization of juvenile GIFT tilapia was 3.8 g/kg of diet. 相似文献
103.
经过175 d养殖,对4个N-乙基-N-亚硝基脲(ENU)诱变草鱼家系进行生长对比,采用显著性比较,偏相关分析和因子分析统计方法对草鱼体质量、全长、体长、头长、体高、尾柄长、尾柄高、体厚性状进行分析,进而运用双向测序法对MSTN1、MSTN2基因在具有明显差异家系中进行SNP位点筛选。试验结果显示,家系4的生长速度明显大于家系3,家系4的8个性状明显大于其他3个家系;家系1中体长、尾柄长、体厚与体质量密切相关,家系2中体长、头长、体厚与体质量密切相关,家系3中全长、体长、尾柄高与体质量密切相关,家系4中全长、体长、体厚与体质量密切相关。由此可知,家系4和家系3具有明显的生长差异。对于MSTN1基因,家系3在465位点C/G、467位点G/A,家系4在465位点C/G均发生错义突变;对于MSTN2基因,家系3和4在912位点C/T均发生同义突变,家系3在1027位点G/A、家系4在366位点A/G均产生错义突变,位于非编码区1390位点A/T和1401位点G/A在两个家系均发现SNP位点。试验结果表明,MSTN1、MSTN2基因的不同SNP位点与ENU诱变草鱼的生长性状存在紧密联系。 相似文献
104.
鳡肌肉生长抑制素(MSTN)基因的克隆及其组织特异性表达分析 总被引：1，自引：1，他引：0
以鳡肌肉总RNA为模板,采用RTPCR和RACE的方法,获得了鳡肌肉生长抑制素(myostatin,MSTN)基因的3个重叠片段,测序后拼接得到2 177 bp全长cDNA序列,其包含了5′端非翻译区的89个核苷酸和3′端非翻译区1 052个核苷酸以及1 125个核苷酸的开放性阅读框,翻译编码375个氨基酸,其中前22个氨基酸为鳡MSTN的信号肽。鳡MSTN具有MSTN的共同特征,有蛋白酶水解位点RIRR和在C端生物活性区含9个保守的半胱氨酸残基。核苷酸和氨基酸同源性分析发现鳡MSTN与厚颌鲂、草鱼和鲤等鲤形目鱼类的同源性较高,与鲈形目的同源性较低,与哺乳动物和鸟类的同源性最低;系统发育分析表明鳡MSTN与厚颌鲂亲缘关系最近。 半定量RT-PCR分析表明,该基因在肌肉和脑中表达量最高,在心肌中也有较高表达,在肝脏、肠、鳃和肾等组织中未见明显表达,此结果表明鳡MSTN基因除对肌肉生长发育有调控作用以外,可能还有其他功能。 相似文献
105.
Kyoung Sun Kim Young‐Ji Kim Jeong Min Jeon Yang Soon Kang Young Shil Kang Chul Woong Oh Hyun‐Woo Kim 《Aquaculture Research》2010,41(11):e862-e871
Myostatin is one of the transforming growth factor (TGF)‐β family members and plays inhibitory roles in the development and growth of muscle in mammals. Mammalian myostatins have been studied intensively, considering its medical and industrial potential use. Still, limited information is available about myostatin homologues in crustaceans. In the present study, we isolated for the first time cDNA that encodes for myostatin‐like protein (Pj‐MSTN) from Morotoge shrimp, Pandalopsis japonica. The putative mature peptide of Pj‐MSTN was composed of 109 amino acids, which contains an additional amino acid residue compared with mammalian myostatins. Pj‐MSTN exhibited 32% amino acid sequence identity and 52% similarity to human myostatin. Multiple sequence alignment analysis indicated that Pj‐MSTN shared the conserved proteolytic cleavage site (RXXR) for its maturation and nine cysteine residues for disulphide bridges. These results suggest that Pj‐MSTN has conserved the three‐dimensional structure of TGF‐β family members in vertebrates. Phylogenetic analysis suggests that Pj‐MSTN is a primitive form of vertebrate myostatin and GDF11. The expression of Pj‐MSTN was not just identified in muscular tissues, suggesting that Pj‐MSTN functions differently from mammalian myostatin. Ablation of the X‐organ/sinus gland complex significantly reduced the expression of Pj‐MSTN in most tissues, suggesting its potential association with moulting. 相似文献
106.
107.
LIUDi YANGXiu-qin YANGJia-fang 《东北农业大学学报(英文版)》2004,11(2):148-150
Myostatin, with a highly conservative gene among breeds is a negative regulator of muscle. The 3‘coding regions of wild boar and crossbred pig myostatin were cloned by RT-PCR and sequenced respectively. Thehomology of the nucleotide sequence between wild boar and crossbred pig was 100% and there was no difference in this region compared with pig myostatin gene of Genbank. This indicated that there was not change of gene sequence in this region during the evolution processes. 相似文献
108.
猪肌生成抑制素C末端80氨基酸编码基因的合成及其原核表达 总被引：1，自引：0，他引：1
将猪肌生成抑制素编码序列下游883～1 126 bp按大肠杆菌嗜好密码子进行优化,将优化后序列双链分成12个单链片段(F1、F2、F3、R6、R5、R4、F4、F5、F6、R3、R2、R1),采用化学合成方法合成,每对相邻互补片段之间有20 bp序列交叉重叠.F1长38 bp,R1长36 bp,其他片段均40bp长,F1和R1片段两端分别加上限制性内切酶Nco I和Xho I的识别位点序列.经PCR扩增出254 bp片段,该片段与pMD18-T载体连接,转化JM109感受态细胞,所得阳性克隆进行测序分析,得到的克隆序列与设计的序列完全一致,表明成功地获得了猪肌生成抑制素下游编码序列克隆载体.从阳性细菌中提取质粒,经Nc0 I和Xho I酶切,回收254 bp目的片段,定向克隆到pET28a中,转化DH5.受体菌,提取质粒,再转化到BL21(DE3)中,成功筛选出阳性克隆.阳性菌经IPTG诱导,通过SDS PAGE,检测出猪肌生成抑制素的表达. 相似文献
109.
Nucleotide sequence of myostatin gene and its developmental expression in skeletal muscles of Japanese Black cattle 总被引：1，自引：0，他引：1
Masahiro SHIBATA Kazunaga OHSHIMA Takatoshi KOJIMA Takayuki MURAMOTO Kazunori MATSUMOTO Masanori KOMATSU Katsuhiro AIKAWA Shinobu FUJIMURA Motoni KADOWAKI 《Animal Science Journal》2003,74(5):383-390
Myostatin, a member of the transforming growth factor‐β superfamily, is a well known negative regulator of skeletal muscle growth. In the present study, the 6660 bp nucleotide sequence of the myostatin gene in Japanese Black cattle (JBC), including the entire coding region of 1128 bp, was determined. The amino acid sequence deduced from the nucleotide sequence of JBC was well conserved with its sequence of other cattle, although it was found that an Α→G transition at nucleotide position 641 results in the substitution of asparagine by serine at amino acid position 214. In order to examine the expression pattern of the myostatin gene in the skeletal muscles of JBC, its expression in three skeletal muscles, Semitendinosus (ST) muscle, Biceps femoris muscle and Longissimus lumborum muscle, of fetal and calf stages was analyzed by real time polymerase chain reaction. The highest level of the myostatin expression was observed in the fetal stage. In calf stages the highest expression was observed in ST muscle compared with the other two muscles. These results suggest that a higher expression of myostatin gene, especially in the fetal stage and in ST muscle during calf stages, is involved in the arrest in skeletal muscle growth and that its functional domains and genomic structure in JBC are well conserved with those in other mammals. 相似文献
110.
通过研究公、母鸽肉质性状候选基因肌肉抑制素(Myostatin,MSTN)在不同组织、不同发育阶段的表达变化规律,为研究鸽肉质性状的遗传调控机理提供依据。采用荧光探针RT-PCR,定量分析MSTN mRNA在公、母鸽的多个组织、多个发育时间点的表达量。RT-PCR结果表明,MSTN mRNA表达量在3个生理时期表现出先降低后升高的变化趋势,母鸽峰值出现较早。公鸽MSTN mRNA表达量最高的组织为肾脏,睾丸中表达丰度最低,青年期肝脏、心肌MSTN mRNA表达量极显著高于其它时期的表达量,但在睾丸中没有检测到基因的表达。对不同生理时期母鸽MSTN mRNA的表达量比较后发现,青年期MSTN mRNA表达最高,显著高于其它时期。MSTN mRNA表达量受到组织、发育阶段和品种影响,该基因的表达在物种之间存在差异。 相似文献