Frost hardening spruce container stock for overwintering in Ontario     

Colombo  Stephen J. 《New Forests》1997,13(1-3):449-467

Difficulties overwintering container stock in northern Ontario led to the development of the extended greenhouse culture hardening regime for spruce seedlings. Laboratories to measure shoot frost hardiness and evaluate terminal bud development were established to monitor nursery crops being hardened using this regime. Information on frost hardiness and bud development provided by these laboratories has been used by nursery managers to determine readiness of container seedlings for overwintering. Since 1982, over 200 stock lots have been monitored by these operational laboratories. This database can be used to determine the importance of nursery cultural factors and seed source on frost hardening. The database shows large differences between nurseries in approach to hardening seedlings which were reflected in levels of freezing damage, winter desiccation and overwintering success. Rates of frost hardening (i.e., the interval between terminal bud initiation and attainment of a --15°C level of shoot frost hardiness) of crops produced in north central Ontario failed to show significant seed source effects. The rate of frost hardening was faster in crops producing fewer needle primordia in terminal buds.  相似文献   

松针内含物与马尾松毛虫生存发育关系的研究   总被引：10，自引：4，他引：10  

邹运鼎  程扶玖 《林业科学》1990,26(2):142-148

本文用不同松树松针饲养马尾松毛虫,其结果与松针内含物的关系,经计算得出累计死亡率、化蛹率、羽化率、每个雌虫平均产卵率、周限增长率以及硬脂酸、油酸等14种。同时得出:维生素B_6(x_(13))对马尾松毛虫的化蛹、羽化和周限增长率的影响是第一位的重要因素,低沸点物质(x_8)包括萜烯类物质是影响马尾松毛虫化蛹、羽化的第二因子,苏氨酸(x_(21))是第三个因子。异亮氨酸(x_(22))是影响马尾松毛虫产卵量的第一重要因子,蛋氨酸(x_(20))是第二重要因子,维生素B_6是第三个重要因子。  相似文献   

Theory Model of Viscoelasticity of Hepatocellullar Carcinoma Cells by Micropipette Aspiration Method     

SONG Guan bin  ZHAO Yan hu  LONG Mian  WU Ze zhi  WANG Yuan liang  WANG Bo chu  CAI Shao xi 《保鲜与加工》2003,(4):104-107111

The mechanical properties of cells are relation to their structure and function. Viscoelasticity is a kind of essence properties of cells and an important researching contents in the field of cellular mechanics. Micropipette aspiration technology is an advanced artifice on the level of single cell to study the mechanics of cells. In order to investigate the theory and method in researching mechanical properties of hepatocellullar carcinoma cells with micropipette aspiration, hepatocellullar carcinoma cells was regarded as an even linear solid and the standard linear solid viscoelastic model was chosen to describe viscoelasticity of hepatocellullar carcinoma cells, theory model of viscoelasticity of hepatocellullar carcinoma cells by micropipette aspiration was established according to the related papers, it will provide methodology reference for studies in this fields.  相似文献   

Correlation between fine-needle aspiration cytology and histopathology in the evaluation of cutaneous and subcutaneous masses from dogs and cats     

Ghisleni G  Roccabianca P  Ceruti R  Stefanello D  Bertazzolo W  Bonfanti U  Caniatti M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):24-30

BACKGROUND: Fine-needle aspiration cytology (FNAC) is commonly used as a diagnostic procedure to evaluate superficial and deep masses in animals. However, few studies have addressed the accuracy of FNAC in the evaluation of cutaneous and subcutaneous masses in a clinical setting. OBJECTIVE: The purpose of this study was to compare the accuracy of FNAC as compared with histopathology in the diagnosis of cutaneous and subcutaneous masses from dogs and cats. METHODS: Cytologic and histopathologic specimens obtained between 1999 and 2003 from 292 palpable cutaneous and subcutaneous masses obtained from 242 dogs and 50 cats were retrospectively evaluated. Cytologic samples were obtained by FNA and histopathologic samples were collected by surgical biopsy or at necropsy. Concordance was determined and the accuracy of FNAC for the diagnosis of neoplasia was determined using histopathology as the gold standard. RESULTS: Of 292 specimens, 49 (from 44 dogs and 5 cats) were excluded due to poor cellularity of the cytologic specimen (retrieval rate 83.2%, n = 243). A cytologic diagnosis of neoplasia was obtained in 176 cases (175 true positives and 1 false positive compared with histopathology). Sixty-seven cytology samples were classified as non-neoplastic (46 true negatives, 21 false negatives compared with histopathology). Overall, the cytologic diagnosis was in agreement with the histopathologic diagnosis in 90.9% (221/243) of cases. For diagnosing neoplasia, cytology had a sensitivity of 89.3%, a specificity of 97.9%, a positive predictive value of 99.4%, and a negative predictive value of 68.7%. CONCLUSIONS: The results of this study confirmed FNAC as a reliable and useful diagnostic procedure for the evaluation of palpable cutaneous and subcutaneous lesions in small animal practice.  相似文献   

Seedling shoot,needle and bud development in three provenances of Pinus sylvestris and Pinus contorta cultivated in northern Sweden     

《Scandinavian Journal of Forest Research》2012,27(1-4):356-363

The patterns of current‐year shoot, needle and terminal bud elongation in seedlings of three Scots pine (Pinus sylvestris L.) and three lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.) provenances were compared during the third and fourth growing seasons after planting. Lodgepole pine produced longer shoots and buds than did Scots pine, mainly because lodgepole pine formed more stem units and elongated at a faster rate. Stem unit length and the duration of shoot and bud elongation differed relatively little between species and provenances. Lammas or polycyclic growth occurred in some lodgepole pine provenances, but not in any Scots pine provenance, and was associated with enhanced shoot elongation. Needle elongation commenced earlier, proceeded at a faster rate, and was greater in lodgepole pine than in Scots pine, but ceased about the same time in all species and provenances. The heat sum required to attain 50% of final length was lower for shoots and needles in lodgepole pine than in Scots pine, and for shoots in northern provenances than in southern ones. Mitotic activity in the apical meristem of the terminal bud, which occurred less than one week after the seedlings were free from snow, started and ceased about the same time in each species, but was higher in lodgepole pine than in Scots pine early in the shoot elongation period.  相似文献   

显微注射针对猪ICSI胚胎的发育和EGFP表达效率的影响     

张静  赵栓  田祯  方园  谭禄彬  曾申明 《中国畜牧杂志》2012,38(3):26-28

本实验以猪体外成熟卵子以及冷冻解冻后失活的精子为材料,以无BSA且成分明确的TL-HEPES溶液为操作液,探讨显微注射过程中分别使用钝口针和磨口针进行注射,对猪卵子的激活、ICSI胚胎的发育及EGFP表达效率的影响。结果表明:成熟后的猪卵子在不进行精子注射和电激活的情况下,使用钝口针空注射后,卵裂率显著高于磨口针(P<0.05)。分别使用钝口针和磨口针对成熟后的猪卵子进行精子注射,不论进行或不进行电激活,使用钝口针注射,卵子的卵裂率、囊胚率和胚胎EGFP的表达效率均显著高于磨口针(P<0.05)。本实验研究发现,使用钝口针进行注射有利于猪ICSI卵子的激活,胚胎的发育以及胚胎中EGFP表达效率。  相似文献   

新疆耐盐小麦"78"、"101"耐盐性的研究     

任崴  罗廷彬  王宝军  苏风春 《干旱地区农业研究》2004,22(3):86-90

中国科学院新疆生态与地理研究所培育出的耐盐丰产小麦新品系“101”、“78”,在耐盐比较试验中具有较强的耐盐能力。在硫酸盐溶液浓度为1．5％时,发芽率高于轮抗6、德选1和对照新冬17;在以氯化物一硫酸盐为主的盆栽试验中,土壤盐分含量为1．5％时,“101”、“78”的三叶期死亡率、旗叶干尖长度低于轮抗6、德选1和对照新冬17,产量高于轮抗6、德选1和对照新冬17。在轻度-中度盐渍化地上,具有较高的生产效益;在中上等肥力的地上,和其他丰产性品种相比,具有较好的效益。“78”属弱冬性,冬前晚播不影响产量,是接棉花茬、玉米茬的优良小麦。  相似文献   

Computed tomography-guided fine-needle aspiration and tissue-core biopsy of bone lesions in small animals     

Massimo Vignoli  DR. MED. VET.  SRV    Stefanie Ohlerth  DR. MED. VET.    Federica Rossi  DR. MED. VET.  SRV    Luigi Pozzi  PROF. DR. MED. VET.    Rossella Terragni  DR. MED. VET.    Daniele Corlazzoli  DR. MED. VET.    Barbara Kaser-Hotz  PROF. DR. MED. Vet 《Veterinary radiology & ultrasound》2004,45(2):125-130

In humans, free-hand computed tomography (CT)-guided biopsy is an accurate method to obtain a tissue sample. There are only a few reports of this technique in veterinary medicine. In the present study, 21 dogs and two cats underwent a free-hand CT-guided tissue-core biopsy (17 animals) or fine-needle aspiration (six animals) of a bone lesion. Two out of 17 tissue-core samples were also cultured. All 17 tissue-core biopsy samples were diagnostic (accuracy of 100%). Five out of six aspirates were diagnostic (accuracy of 83.3%). The overall accuracy was 95.7%. In one aspirate, cytologic quality was insufficient containing only blood. No major complications were encountered. Fourteen neoplastic, two infectious and six benign lesions were diagnosed. CT examination after intravenous contrast medium added useful information to avoid large vessels and to biopsy-viable tissue. Free-hand CT-guided tissue-core biopsy and aspiration appears to be a safe and very accurate procedure for use in the diagnosis of bone-associated diseases in small animals.  相似文献   

松针粉-麦饭石复合添加剂对肉鸡法氏囊生长发育的影响     

王群  王珏  李春鹏 《水禽世界》2005,(5):15-17

试验取144只1日龄健康AA肉仔鸡,随机分为2组,每组72只,组内设2个重复,每个重复36只。对照组饲喂基础日粮,试验组饲喂添加1%松针粉、1%麦饭石的基础日粮,饲养期42d。分别于7、14、21、28、35、42日龄随机取鸡12只,每组6只,各重复3只。颈静脉放血致死,解剖取法氏囊称重,取材制作石蜡切片,光镜观察,显微摄影。结果表明:试验组与对照组鸡法氏囊指数在7、35日龄差异极显著(P<0.01),14、42日龄差异显著(P<0.05);法氏囊小结皮质均显著厚于对照组。本试验从组织学角度证明,在基础日粮中添加松针粉和麦饭石可显著促进肉鸡法氏囊的早期发育,延缓退化的时间。  相似文献   

松科植物在畜牧生产中的应用     

王占彬  白喜婷  董发明 《中国饲料》2005,(17):27-29

我国松科植物资源相当丰富,但目前畜牧业生产上的利用主要是部分松针,大量松针、松树皮、嫩枝被浪费,因此,开发利用这些资源对于发展我国畜牧业具有重要的现实意义。本文论述了松科植物资源的利用现状、存在问题及其提取物的作用机理。  相似文献