商陆根部多糖的分离、纯化及其体外抗氧化活性     

段静  张继千  王立宽  孟凡君 《广西农业生物科学》2010,(3):523-528

本研究从商陆根部提取分离出商陆多糖,并对该多糖含量进行测定及体外抗氧化活性研究。商陆根部经石油醚回流脱脂、热水抽提、脱色、乙醇沉淀等处理可从中提取出粗多糖。多糖经三氯乙酸脱蛋白纯化,再分别以考马斯亮蓝法和蒽酮-硫酸法测定其蛋白质含量和糖含量,并通过采用清除羟自由基和超氧自由基能力测定法、β-胡萝卜素-亚油酸法,以抗坏血酸（Vc）为对照,来分析粗多糖和纯多糖的体外抗氧化能力。采用水提法提取的粗多糖,含有10.69%的蛋白质和60.91%的多糖;纯化后的多糖糖含量达到91.04%。商陆粗多糖、纯多糖及Vc对羟自由基（·OH）清除率的IC50分别是1.26mg/mL、4.78mg/mL和0.224mg/mL;对超氧自由基（O2-·）清除率的IC50分别为1.91mg/mL、2.28mg/mL和0.123mg/mL;对β-胡萝卜素-亚油酸体系的抑制作用的IC50分别为0.471mg/mL、0.692mg/mL和0.379mg/mL。实验结果表明商陆多糖是一种较好的抗氧化剂,可作为潜在天然抗氧化剂应用于食品和医药工业中。  相似文献   

氮水平对水稻植株氮素损失的影响   总被引：7，自引：1，他引：6  

王巧兰  吴礼树  赵竹青  赵林萍 《植物营养与肥料学报》2010,16(1):14-19

利用15N差值法,在溶液培养条件下研究了不同氮肥水平对水稻植株氮损失的影响,并就影响水稻氮损失的因素进行了分析。结果表明,对前期正常供氮的水稻幼苗做为期10 d的不同氮(N 04、0、801、60 mg/L)处理,水稻植株生物量未受显著影响,表明前期吸收氮可维持水稻生长。但是,随着供氮水平的提高,叶片及根的含氮量显著增加,而15N的丰度却显著下降,叶片15N的丰度显著高于根。说明高氮处理增加了水稻植株吸氮量并稀释了前期吸收的15N,而且根系累积的氮向地上部转移。缺氮(N 0 mg/L)与过量供氮(N 160 mg/L)均显著增加植株氮的损失率,而适量供氮(N 80 mg/L)则氮肥利用率显著提高。水稻的生长期显著影响植物氮的损失率,在N 80 mg/L的条件下,随着水稻生长期的延长,植株氮损失从11.6%增加到22.3%。同时,随着供氮水平的增加,叶片中NH4+-N含量和谷草转氨酶(GOT)活性均显著增加,叶片组织pH也随之增加。表明植物体内铵浓度增加而引起的氨挥发是导致植物氮损失增加的原因之一。  相似文献   

4种花灌木乔木化育苗技术研究     

黎可华  李志东  陆尚智  梁锦钧 《广东园林》2011,33(1):61-65

为了摸索如何把花灌木育成树状小乔木的育苗技术,选择了园林常见多花的扶桑、红花夹竹桃、黄花夹竹桃、紫薇等4个树种,采用保留单一中轴主枝,并按主枝全苗、主枝中截和主枝截顶三个处理方法,通过头年密植育干,第二年疏植养干,第三年储冠的育苗技术,育成了树状小乔木,改进了育苗期整形修剪技术.进一步提高了园林观赏效果.  相似文献   

Antioxidant Activity of Pigment Extracted from Green-Wheat-Bran   总被引：1，自引：0，他引：1  

TANG Xiao-zhen  DONG Yu-xiu  WEI Si-qin  ZHANG Xian-sheng  YIN Yan-ping 《中国农业科学(英文版)》2010,9(6):825-832

The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.  相似文献   

军牧1号白猪、杜洛克猪和藏猪的氟烷基因和酸肉基因多态性分布     

印崇  孙博兴  赵志辉  杨润军  白春艳  邢沈阳 《黑龙江畜牧兽医》2012,(1):7-9

为了研究军牧1号白猪、杜洛克猪和藏猪氟烷基因和酸肉基因的多态性分布情况,试验采用PCR-RFLP方法对61头军牧1号白猪、51头杜洛克猪和51头藏猪的氟烷基因和酸肉基因多态性进行了检测。结果表明:军牧1号白猪的氟烷基因表现为单一的NN型;杜洛克猪的氟烷基因表现为多态性,等位基因N的频率为0.196 1,基因型分布符合哈代-温伯格平衡(χ2=3.033 9,P=0.081 5);藏猪的氟烷基因表现为单一的nn型。3个猪种的酸肉基因均表现为单一的rn/rn型。  相似文献   

新围海涂农田林网造林技术     

黄志刚  叶根法 《浙江林业科技》1999,19(4):58-61

9年来，上虞市海涂综合开发区的农田防护林建设实践表明，新围海涂防护林营造技术的关键是应根据不同树种的生物学特性，因地制宜，适地适树，进行正确合理的配置；采取排水洗盐工程措施，改善立地条件；严格整地，大穴移栽；采用农艺措施，改善苗木立地环境。从而使区内的森林覆盖率增加4％～10％，有效地改善了农田生态环境，提高了农业综合开发效益。  相似文献   

A laboratory investigation of the release of a conservative tracer and herbicide from topsoil aggregates under varying rainfall intensities     

C.M. Heppell  T.P. Burt  R.J. Williams  A.C. Johnson 《Soil Use and Management》2000,16(3):175-182

Abstract. This paper describes the results of laboratory-based research which investigated the losses of the herbicide, isoproturon, and a non-adsorbing solute, bromide, from topsoil aggregates under high (7 mm h⁻¹ and low 2.7 mm h⁻¹) intensity simulated rainfall. The structures of the micro- and macro-aggregates from a clay soil of the Denchworth Series (from Wytham, Oxfordshire, UK) were observed using a scanning electron microscope, and the slaking of the topsoil aggregates was also investigated in order to provide a context for interpreting the experimental results. The topsoil at Wytham was found to have a bimodal structure with small microaggregates of 0.71 to 1 mm diameter clustered together to form larger macroaggregates of up to several centimetres diameter. Selected aggregates were air dried and then repacked in Buchner funnels to several centimetres depth in order to remove variability in the experimental results due to the effects of surface microrelief. Under saturated surface conditions high intensity simulated rainfall was less efficient at removing both non-adsorbing and adsorbing solutes from the repacked topsoil aggregates than low intensity rainfall. The observed decrease in the concentration of isoproturon and bromide in the leachate with time could be explained by a transport non-equilibrium effect.  相似文献   

杜香油主要成分的分离和鉴定   总被引：5，自引：1，他引：4  

邱志国  马慧媛 《东北林业大学学报》1997,25(1):41-44

用减压蒸馏、气相色谱分离、红外和紫外光谱法、催化加氢和沸点测定等手段对杜香油中三种含量较高的成分进行了分离和鉴定。结果确定它们是月桂烯、对－伞花烃、桃金娘烯醛。并参考同条件下的ＧＣ与ＧＣ－ＭＳ结果鉴定出α－蒎烯、茨烯等化合物。测定了各成分在杜香油中的含量。  相似文献   

An analysis of age and body weight at first calving for Holsteins in the United States     

Willard C. Losinger  A. Jud Heinrichs 《Preventive veterinary medicine》1997,32(3-4):193-205

In a national survey of US dairy producers, only 2.7 ± 0.5% of Holstein dairy operations reported achieving recommended target ranges of age ≤ 24 months and body weight (BW) ≥ 550 kg at first calving. Allowing for wider target ranges, still only 14.6 ± 1.3% of Holstein dairy operations reported achieving age ≤ 25 months and BW ≥ 544.3 kg at first calving. Ages of individual first-calf heifers observed at calving were heavily skewed toward older individuals. Dairy producers reported an average age at first calving that was 1.3 months lower than the mean and 1.0 months lower than median age of first-calf heifers' observed calving on the operations. Stepwise logistic regression was used to identify the herd characteristics associated with producers reporting first calvings within the wider age and BW target ranges for Holsteins. Rolling herd average milk production ≥ 7711 kg/yr, using a computer for recordkeeping, and not tying preweaned heifers in a barn with cows, were associated with achieving the target BW and age at first calving.  相似文献   

催青环境及分段收蚁对原蚕性别调控研究          下载免费PDF全文

钟勇玉  薛三勋  张百忍  付先锋  王友玲  王晓文 《西北农业学报》2001,10(4):14-16

试验结果表明 ,通过改变催青环境和分段收蚁可以人工调控家蚕的性别比例。在原种催青期中高温干燥处理 ,可使原蚕品种 872和鲁玉 蚕比例分别提高 1 3 %和 2 4% ,达极显著和显著水平 ;对原蚕 795和871品种分段收蚁 ,第 1天可提高♀性比例 1 0 %～ 2 0 % ,最后一天 (第 3天 )提高 蚕比例 ,795品种最高为2 8%～ 3 6% ,达极显著水平。  相似文献