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51.
This experiment was conducted to study the effects of dietary nonspecific IgY on growth performance, immune function and microbiota number of chicks, to provide the theory basis for practical application as feed additive in livestock and poultry. 240 one-day-old Roman rooster hens with same batch of hatching and similar body weight were randomly divided into 2 groups (control group and experimental group), with 6 replicates in each group and 20 chicks in each replicate. Chicks in the control group were fed with a basal diet, and the others in the experimental group were fed the basal diet supplemented with 0.1% nonspecific IgY. The experiment lasted for 6 weeks. At the age of 6 weeks, 2 chicks from each replicate were weighed and slaughtered, and the development of internal organs, immune index and microbiota number were determined. The results showed that:Comparing with the control group, the weight of chicks at the age of 1 and 5 weeks, the glandular stomach weight, spleen weight, serum IL-2 content, jejunal sIgA content and cecal Lactobacilli number in experimental group were significantly increased (P < 0.05). In conclusion, dietary supplementation of nonspecific IgY could enhance growth performance, digestive organ development, immune function, and intestinal bacteria number. 相似文献
52.
测定了海州湾、辽东湾、舟山沿海和莱州湾4个野生群体三疣梭子蟹(Porttmus tritubercalatus)的血淋巴、肌肉和肝胰腺组织中的抗氧化及其它与非特异性免疫相关的酶活指标,并对这些指标在不同群体间的差异进行了比较.结果发现:海州湾群体的三疣梭子蟹肝胰腺SOD酶活显著高于莱州湾群体(P<0.05).而与其它两个群体差异不显著;而血淋巴和肌肉组织SOD酶活各个群体间差异均不显著(P>0.05).海州湾群体血淋巴和肝胰腺组织GSH-px酶活显著高于莱州湾群体(P<0.05),而与其它群体差异不显著(P>0.05).不同地理群体三疣梭子蟹各体组织中MDA含量、LSZ和ALP酶活均没有显著性差异(P>0.05).笔者全面比较了4个地理群体三疣梭子蟹免疫相关酶活指标的差异,揭示了不同地理群体三疣梭子蟹免疫、抗病能力差异的内在因素. 相似文献
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The stimulatory effect of fermented vegetable product (FVP) upon the phagocytic and superoxide generation of leukocytes was
studied in the Japanese flounder Paralichthys olivaceus. The phagocytic activity of casein-induced, intraperitoneal leukocytes was investigated and quantified, that is the activity
significantly increased (P<0.05 or <0.01) by the addition of FVP beyond 3 mg/kg body weight. Further analysis investigated the effect of FVP on superoxide
generation in leukocytes. Established in vitro cytochrome c reduction assay was used to measure superoxide generation; reduced levels of FVP in assay samples had a profound
effect on superoxide generation. FVP was also incorporated in commercial diets and fed to Japanese flounder for 4 weeks. The
phagocytic activities and superoxide generation of peritoneal induced leukocytes were significantly higher (P<0.05, <0.01) in fish fed the FVP supplemented diet than fish fed the control diet. FVP feeding in fish had a significantly
higher (P<0.05) activity of lysozyme than in the control fish. 相似文献
55.
This study was carried out to compare growth, intestinal enzymes activity, antioxidation and nonspecific immunity of loach between farmed and imitative ecological farming loach, Misgurnus anguillicaudatus. Loach from farmed (average body weight 15.03 ± 0.36 g) and imitative ecological farming (average body weight 12.44 ± 0.62 g) population were used to analyse growth, whole-body composition, intestinal enzymes, antioxidant enzymes activity and resistance to bacteria. The results showed specific growth rate, average daily gain and protease, lipase, amylase and γ-GT activities in intestine and hepatopancreas of farmed loach were significantly higher (p < 0.05) than of imitative ecological farming loach. However, total protein, lysozyme and albumin contents and superoxide dismutase, catalase and glutathione peroxidase activities of imitative ecological farming loach were significantly higher (p < 0.05) than of farmed loach. Moreover, resistance to Aeromonas hydrophila of imitative ecological farming loach was higher than of farmed loach (p < 0.05). Taken together, ecological farming loach possesses higher antioxidant capacity and resistance to bacteria than farmed loach. 相似文献
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饲料中添加壳寡糖和/或霉菌毒素吸附剂对凡纳滨对虾生长性能、非特异性免疫力及抗病力的影响 总被引：1，自引：0，他引：1
本试验旨在研究在饲料中单独或联合添加壳寡糖(COS)和霉菌毒素吸附剂(MA)对凡纳滨对虾(Litopenaeus vannamei)生长性能、非特异性免疫力及抗病力的影响。首先配制基础饲料(C0组,作为对照组),然后在基础饲料中分别添加100 mg/kg COS(C0.1组)、250 mg/kg COS(C0.25组)、2 500 mg/kg M A(M 2.5组)、100 mg/kg COS+2 500 mg/kg M A(C0.1+M 2.5组)、250 mg/kg COS+2 500 mg/kg MA(C0.25+M2.5组),共制成6种等氮等脂的试验饲料,投喂初重为(0.23±0.02)g的凡纳滨对虾8周。每组设3个重复,每个重复放养40尾对虾。结果表明:1)C0.1+M2.5组的特定生长率、增重率显著高于M2.5组(P0.05),C0.1+M2.5组的饲料系数最优,显著低于其他各组(P0.05),C0.1+M2.5组的蛋白质效率最高并显著高于除C0.25+M 2.5组外的其他各组(P0.05);C0.1+M 2.5组的虾体粗蛋白质含量较C0.1组显著升高(P0.05),虾体粗脂肪含量较C0.1组显著降低(P0.05)。2)C0.1+M 2.5、C0.25+M 2.5组血清中碱性磷酸酶(AKP)活性均显著低于C0.1和C0.25组(P0.05),C0.1+M2.5组血清中超氧化物歧化酶(SOD)、酚氧化酶(PO)活性均显著高于M2.5和C0.1组(P0.05),C0.1+M2.5组血清中丙二醛(MDA)含量显著低于C0.1组(P0.05);C0.1+M2.5组肝胰腺中MDA含量显著低于C0.1组(P0.05),C0.25+M 2.5组肝胰腺中AKP、PO、SOD、溶菌酶(LZM)活性比C0.1+M 2.5组均有降低,相反肝胰腺中MDA含量则有所升高。3)以哈维氏弧菌攻毒7 d后,C0.1+M2.5及C0.25+M 2.5组的累积死亡率均显著低于C0和M 2.5组(P0.05)。由结果可知:本试验条件下,饲料中添加一定量的COS和/或MA均能促进凡纳滨对虾的生长,联合添加COS与MA的促生长、提高免疫力和抗病力的效果优于单独添加COS和MA,综合来看,以100 mg/kg COS和2 500 mg/kg M A联合添加时作用效果较佳。 相似文献
60.
Chenchen Jia Xiaozhen Zhu Xianyao Meng Yuetao Yi 《Journal of the World Aquaculture Society》2020,51(1):200-213
This study investigated how Jerusalem artichoke powder (JAP) affected the growth, immunity, and gut microbiota of the sea cucumber, Apostichopus japonicus Selenka. Animals were fed diets containing antibiotic (1 ppm) or five levels of JAP (0, 2.5, 5, 10, and 15 g/kg). After the feeding trial, three sea cucumbers were sampled per tank to assay for immunity and gut microbiota composition. Dietary supplementation of 2.5 g/kg JAP significantly improved the growth rate and phenoloxidase activity of sea cucumbers. The diet containing 5 g/kg of JAP increased the survival rate and phagocytic activity, and the diet containing 15 g/kg of JAP increased the growth rate and total coelomocyte count of sea cucumbers. Moreover, exposure to antibiotics significantly changed the microbiota of sea cucumbers, while the JAP supplementation stimulated the development of microbiota communities, which could be more homogeneous. Supplementing the diets with 15 g/kg of JAP increased the growth of Rhodobacterales, and this may underlie the higher growth rate of sea cucumbers in this treatment. Considered together, our results suggest that supplementing the diet of sea cucumber with JAP may be beneficial for nonantibiotic farming of sea cucumber. 相似文献